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Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and predesigned TaqMan probes (Applied Biosystems, UK) for reference gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cell Biology 2024Quote: ... permeabilized for 30 min with 0.5% Triton X-100 in PBS and incubated with Alexa Fluor 594 Phalloidin (1:100; Molecular Probes, UK; A12381) and DAPI for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... The crude mucus preparation was diluted to OD280 0.1 in PBS and added to each well of a Nunc Maxisorb plate (Nalgene-Nunc, Thermo Fisher Scientific, Rochester, NY, USA) which was then incubated at 4°C overnight under slow rotation ...
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bioRxiv - Microbiology 2024Quote: ... in 0.5M NaOAc (Invitrogen AM9740) was used to stain nucleic acids for loading control ...
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bioRxiv - Microbiology 2024Quote: ... All qPCR reactions were run on a QuantStudio 3 qPCR machine (Applied Biosystems).
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bioRxiv - Microbiology 2024Quote: ... and the final concentration was determined by fluorometric analysis using the Quant-iT dsDNA HS Assay Kit (Invitrogen) on a Fluoroscan plate reader (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: The protein concentrations of MV preparations were quantified using Qubit protein kit (Invitrogen Carlsbad, CA, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... 1 μl of RNase inhibitor (ThermoFisher #EO0381), and 1 μl of SSIV enzyme ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μlof GlycoBlue co-precipitant (ThermoFisher #AM9516). Three volumes of 100% ethanol were added to the RNA-containing buffer for overnight precipitation ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 10% fetal bovine serum (Gibco) and penicillin and streptomycin (Sigma) ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... HeLa cells were transfected with the resultant plasmid using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA). Single colonies were picked after selection with 0.3 μg/ml puromycin for 3 days ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... with Neon Transfection System (Thermo Fisher Scientific), cells were incubated in DMEM supplemented with 10% FBS for 24 h ...
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Bilateral regulation of EGFR activity and local PI dynamics observed with superresolution microscopybioRxiv - Cell Biology 2024Quote: ... EGFR KO cells were transfected with DEST40/EGFR-rsKame by Lipofectamine 3000 (Thermo Fisher Scientific) and were cultured in the presence of 1 mg/ml G418 (Nacalai Tesque Inc. ...
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bioRxiv - Microbiology 2024Quote: ... SuperScript III (ThermoFisher #18080044), SuperScript IV (ThermoFisher #18090050 ...
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bioRxiv - Cell Biology 2024Quote: ... the eyes were washed five times in Hanks’ Balanced Salt Solution (HBSS Cat #14025092 Gibco by Life Technologies, Grant Island, NY) and stored for up to 24 hours before dissection at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and covered with a 22X40mm coverslip (Thermo Fisher #152250).
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bioRxiv - Cell Biology 2024Quote: ... to a SuperFrost Plus microscope glass slide (Fisher Scientific #12-550-15). The flat mount was then rinsed with HBSS and incubated in blocking buffer made with HBSS with 5% bovine serum albumin (Catalog #BP9703-100 ...
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bioRxiv - Cell Biology 2024Quote: ... the eyes were washed five times in Hanks’ Balanced Salt Solution (HBSS Cat #14025092 Gibco by Life Technologies, Grant Island, NY) and stored for up to 24 hours before dissection at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... diluted in 1X PBS (Fisher Scientific #50-980-487 and Corning 46-013-CM) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA isolation was performed using ChargeSwitch™ gDNA Plant Kit (Invitrogen) according to the manufacturer’s instructions and the quality and concentration were checked using agarose gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% Fetal bovine serum (Gibco, USA), 1% antibiotic mixture (penicillin-10,000 U/ml and streptomycin-10 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... and 10 mg/L gentamicin (Invitrogen 15750060). Parasites were grown at 2% hematocrit in human red blood cells obtained from the University of Utah Hospital blood bank ...
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bioRxiv - Microbiology 2024Quote: ... or 50 nM mirVanna inhibitor negative control#1 (#4464077, ThermoFisher Scientific: confirmed by ThermoFisher to have no binding sites in P ...
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bioRxiv - Microbiology 2024Quote: ... aeruginosa exposed to vehicle or tobramycin (1 μg/ml) according to the manufactures protocol (ThermoFisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... and 20 µL of the supernatant was loaded and run on a NativePAGE 4-16% Bis-Tris gel (Invitrogen BN1002BOX) and transferred to PVDF membrane (pre-wetted in 70% methanol) ...
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bioRxiv - Microbiology 2024Quote: ... aeruginosa (strain PA14) and all CF clinical isolates were grown in luria broth (LB, ThermoFisher Scientific, Waltham, MA) liquid cultures at 37°C with shaking at 225 rpm for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... incubated with 8 µM of FITC-phalloidin (Invitrogen), washed with PBS and Saline-Sodium Citrate buffer (SSC ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplification and detection of 5′ tRNA-fMet halves were performed using the TaqMan Universal PCR Master Mix (#4304437, ThermoFisher Scientific) as well as custom primers and probe design to target both 5′ tRNA-fMet halves specifically.
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bioRxiv - Microbiology 2024Quote: ... DNA was removed using DNAse (Invitrogen, USA), and the reverse transcription was performed using Superscript FirstStrand Synthesis for RT-PCR (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... or 50 nM mirVanna inhibitor negative control#1 (#4464077, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... and 1% non-essential amino acids mixture (Life Technologies, USA), in an atmosphere of 5% CO2 at 37°C for up to 10 days ...
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bioRxiv - Microbiology 2024Quote: ... One µg of RNA was DNAse-treated and reverse-transcribed using Superscript IV kit (Invitrogen) with the addition of 1 µM gene-specific reverse primers HCCSqR1 ...
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bioRxiv - Microbiology 2024Quote: ... then quantified using Qubit™ 4 fluorometer and dsDNA HS Assay Kit (Thermofisher Scientific, Waltham, MA, USA). Isolated DNA was stored at -20°C until library preparation ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Microbiology 2024Quote: ... Mucin 5AC for goblet cells (1:1000, Invitrogen, Catalog number: 45M1), goat polyclonal antibody specific for RSV (1:2000 ...
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bioRxiv - Cell Biology 2024Quote: ... and D-(+)-glucose were purchased from Sigma and L-glutamine from ThermoFisher.
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... GDH and ALDH18A1 using RNAiMax lipofectamine (Invitrogen) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: GSDMD – GGA ACU CGC UAU CCC UGU Utt (Thermo Fisher; siRNA ID s36339)
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bioRxiv - Microbiology 2024Quote: ... Parasitemia was monitored daily by flow cytometry by diluting 10 µL of each resuspended parasite culture well from each replicate into 200 µL of 1.0 µg/mL acridine orange (Invitrogen A3568) in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... incubated with secondary antibodies (donkey anti-mouse 488 Invitrogen, A-21202, donkey anti-rabbit 568, Invitrogen, A-10042 ...
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bioRxiv - Microbiology 2024Quote: ... incubated with secondary antibodies (donkey anti-mouse 488 Invitrogen, A-21202 ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was isolated from frozen pellets and washed using a modified Trizol (Invitrogen) extraction protocol ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM EDTA (Invitrogen AM9261] at 37°C for 2.5 hrs (600 rpm shake) ...
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bioRxiv - Microbiology 2024Quote: Cells were then rinsed once with prehybridization buffer (50 % deionized formamide [Invitrogen] ...
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bioRxiv - Microbiology 2024Quote: ... The crude mucus preparation was diluted to OD280 0.1 in PBS and added to each well of a Nunc Maxisorb plate (Nalgene-Nunc, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 1 μl of 1U/μl bacterial alkaline phosphatase (ThermoFisher #18011015) was added to the reaction and the reaction continued for 1 hr at 37° C ...
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bioRxiv - Microbiology 2024Quote: ... on a Fluoroscan plate reader (Thermo Scientific, Rochester, NY, USA), according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2024Quote: ... 10 to 20 μg of RNA containing 2x RNA loading dye (ThermoFisher #R0641) were heated for 1 min at 95° C before loading onto a pre-run 10% UREA-PAGE gel ...
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bioRxiv - Microbiology 2024Quote: ... Standards for viral copy number were created by cloning PCR product using custom BdDV-1 primers into the pCR 2.1-TOPO vector using the TOPO TA cloning kit (Invitrogen). Transformed Escherichia coli colonies with successful insert were plasmid extracted using the Zyppy Plasmid Miniprep kit (Zymo Research) ...