Labshake search
Citations for Thermo Fisher :
9051 - 9100 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... placenta tissue was homogenized in ice-cold RIPA buffer containing protease and phosphatase inhibitors and protein concentrations determined using Pierce™ Coomassie Plus Assay Kit (Thermo Scientific) following standard protocol ...
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bioRxiv - Microbiology 2022Quote: ... DNA quality was evaluated visually via gel electrophoresis and quantified using a Qubit 3.0 fluorometer (Thermo-Fisher, Waltham, MA, USA) and Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Waltham, MA, USA) for TBC and RUMC samples ...
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bioRxiv - Neuroscience 2022Quote: ... Plates were read using an MSD SECTOR Imager 2400 and resulting peptide concentrations were normalized to total protein in the cell lysate per well measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Data for each shRNA knockdown were additionally normalized to the average of control conditions for each parameter measured.
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bioRxiv - Molecular Biology 2024Quote: ... after which then genomic DNA was isolated for detection of gene editing efficiency using the GeneArt genomic cleavage assay kit (Invitrogen, catalog #A24372). Hep3Bcas9 cells had high cleavage efficiency and thus was chosen for generating knockout cell lines using CRISPR ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each lysate was measured using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). The 2′-3′-cGAMP content were measured by 2′-3′-cGAMP ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each sample was determined by the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). Equal amounts of proteins (up to 20 μg ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA quality and concentration were determined by MOPS-formaldehyde denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... All the OCR and ECAR values were normalized by DNA content measured for each well using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Dublin, Ireland) as previously described.[167]
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bioRxiv - Cancer Biology 2024Quote: Protein translation was detected with the “Click-iT™ Plus OPP Alexa Fluor™ 488 Proteinsynthese-Assay-Kit” (Thermo Fisher Scientific, C10456) according to manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The DCF quantification was normalized on the corresponding protein amount that was quantified using the BCAprotein assay kit (ThermoFisher Scientific, Stockholm, Sweden).
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bioRxiv - Microbiology 2024Quote: ... RNA concentrations were quantified using the Qubit 2.0 Fluorometer and the RNA High Sensitivity Assay Kit (RNA HS, Thermo Fisher, Berlin, Germany).
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bioRxiv - Microbiology 2024Quote: ... the concentration and integrity (RIN) of isolated RNA were determined using the Quant-iT™ RiboGreen™ RNA Assay Kit (Thermo Fisher) and an RNA Standard Sensitivity Kit (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The RT-qPCR assay were performed with a SuperScript III Platinum One-Step RT-qPCR Kit with ROX (Invitrogen - Thermo Fisher Scientific) on a QuantStudio 12K Flex thermocycler (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... The RT-qPCR assay were performed with a SuperScript III Platinum One-Step RT-qPCR Kit with ROX (Invitrogen - Thermo Fisher Scientific) on a QuantStudio 12K Flex thermocycler (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... The concentration of each sample was determined using a Qubit fluorometer and the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32851). To prepare the final libraries ...
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bioRxiv - Genomics 2024Quote: ... the DNA samples obtained were quantified using the Qubit dsDNA BR Assay kit and Qubit 4.0 fluorometer (Life Technologies Corporation, Eugene, OR). As described by Bari et al ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The pellet was resuspended in PBS/EDTA buffer and the protein concentration of the homogenate measured using the Pierce BCA protein assay kit (Thermo Scientific, USA). AChE activity was measured using the Ellman assay using acetylthiocholine as substrate and the indicator 5,5′-dithiobis-2-nitrobenzoic acid (DTNB ...
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bioRxiv - Bioengineering 2024Quote: Cell count was measured on days 1 and 7 using a using Quant-iT™PicoGreen™dsDNA Assay Kit (ThermoFisher Scientific). The scaffolds were washed for 5 minutes in PBS and then frozen at -80⍰C ...
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bioRxiv - Developmental Biology 2024Quote: Protein synthesis was assessed using the Click-iT® L-homopropargylglycine (HPG) Alexa Fluor 488 Protein Synthesis Assay Kit (Molecular Probes, USA), which measures newly synthesized proteins through quantification of the fluorescent HPG signal ...
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bioRxiv - Genomics 2024Quote: Extracted DNA was quantified using a dsDNA broad-range assay kit (Catalogue number: Q32850) on the Qubit 3 fluorimeter (ThermoFisher Scientific UK) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and processed to generate cDNA using 10X Genomics v2 chemistry according to the Chromium Single Cell 3’ Reagents kits v2 user guide.32 After undergoing quantification and quality control of cDNA libraries using the Qubit dsDNA HS Assay kit (Life Technologies Q32851), high-sensitivity DNA chips (Agilent 5067-4626) ...
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bioRxiv - Immunology 2024Quote: Lung homogenates were prepared as described above for TCID50 assays and cytokines were measured using a ProcartaPlex Luminex kit (Thermo Fisher Scientific) on a MAGPIX Instrument (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific). Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... All samples were quantified and normalized using a protein assay kit known as bicinchoninic acid (Thermo Fisher Scientific, Waltham, MA, United States). Following a 10-minute heat treatment at 95 °C ...
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bioRxiv - Microbiology 2024Quote: ... Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) before being used for in vitro acetylation.
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified proteins was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, United States) as described previously (Choi et al. ...
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bioRxiv - Cell Biology 2023Quote: ... was added as a supplement to the lysis buffer and the protein concentration was measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... protein concentrations were measured with the Pierce BCA protein assay kit coupled with the bovine serum albumin standards (23225, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... EdU labeling was performed using the manufacturer’s protocol for the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, catalog no. C10420). EdU incorporation was measured by flow cytometry using the Attune flow analyzer ...
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bioRxiv - Genomics 2023Quote: ... The concentration of DNA for each of the three pools was measured via the Qubit dsDNA HS Assay kit (ThermoFisher, Cat: Q33230). Libraries were prepared for each pool ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after centrifugation (12,000 x g, 20 min) and total protein levels were measured using Pierce™ BCA Protein Assay Kits (Thermo Scientific #23227). Proteins were denatured by boiling with Laemmli buffer (Bio-Rad #1610747 ...
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bioRxiv - Cell Biology 2023Quote: ... were treated with 10 µM EdU for 6 hours before dissociation and staining using the Click-It EdU Alexa Fluor 488 Flow Cytometry Assay kit (ThermoFisher, Cat# C10425).
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bioRxiv - Developmental Biology 2023Quote: ... Extracted DNA and RNA were quantified using a Qubit dsDNA BR (Broad Range) Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA, #Q32853) and a High Sensitivity RNA assay kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... ratios using the Dual Luciferase Reporter Gene Assay Kit (RG027, Beyotime, Shanghai, China) with a Multimode Reader (Varioskan LUX, Thermo Fisher, Finland). Five biological replications were measured for each sample.
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bioRxiv - Neuroscience 2024Quote: ... The protein concentrations in the cytoplasmic and nuclear fractions were determined using a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Cat. 23228). The isolated cytoplasmic and nuclear proteins were aliquoted and stored at -80°C for subsequent analysis.
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bioRxiv - Physiology 2024Quote: RNA-seq was performed at the DNA Core at the CCHMC facility with 10 ng – 150 ng of total RNA used after quantification by Qubit RNA HS assay kit (Cat #Q32852; Invitrogen, Waltham, MA). Based RNA integrity value above 7 determined by the spectrofluorometric measurement RNA samples was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595 ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with O-propargyl-puromycin (OPP) using Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit (Invitrogen) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... then protein concentration was determined using the Pierce™ BCA Protein Assay Kit as per manufacturer’s instructions (cat:23225, Thermo Scientific™). Samples were then processed using the S-trap protocol as per the manufacturer’s instructions54 ...
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bioRxiv - Biochemistry 2023Quote: Protein concentration in venom and antivenom samples was measured using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fraction was removed by 4°C centrifugation at 12000xg for 10 minutes and protein concentration in the soluble fraction was measured by PierceTM BCA protein assay kit (Thermo Fisher Scientific). Protein concentration was adjusted to 1-2 mg/mL for LDBIA treatment.
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bioRxiv - Biophysics 2023Quote: In vitro cytotoxicity of the complexes was evaluated using U-2 OS cells and an LDH assay kit.42 Cells were counted with a Countess II Automated Cell Counter (ThermoFisher Scientific, USA) and seeded in 96-well plates at a density of 10,000 cells per well in 100 μL of full DMEM ...
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bioRxiv - Biochemistry 2023Quote: ... Luciferase assays were performed on aliquots of cleared media harvested from transfected cells using the Pierce Gaussia and Cypridina luciferase flash assay kits (catalog numbers 16159 and 16169 respectively; Thermo Fisher Scientific) and a GloMax 20/20 luminometer (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... The binding affinity of mRNA at two pH values was measured using the Quant-it Ribogreen Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Salts were removed from the supernatant and collected in new reaction microtubes using self-packed C18 STAGE-tips.Peptide concentrations were finally determined with the Micro BCA™ Protein Assay Kit (Thermo Fisher Scientific). 200ng of each sample were analyzed by a LC-MS/MS system consisting in a nanoElute system (Bruker Daltonics GmbH ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations from PBS-soluble (soluble) and sarkosyl-soluble (insoluble) fractions were both assessed using the BCA method (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific). Total protein lysates (50μg ...
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bioRxiv - Immunology 2023Quote: ... a Luminex assay using the ProcartaPlex™ Mouse Cytokine & Chemokine Panel 1 36-plex kit (#EPXR360-26092-901, ThermoFisher Scientific, Waltham, MA) was performed according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The final concentration of the solution was confirmed to be 125 µM using the Pierce™ BCA Protein Assay Kit (23227, Thermo Fisher).
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bioRxiv - Genomics 2023Quote: ... SYBR Green assay: PCR reactions were prepared using the Power SYBR Green RNA-to-CT 1 step kit (Applied Biosystems, Cat# 4391112). Reactions contained a 2× Power SYBR Green RT-PCR Mix ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C) and the protein content of the supernatant was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Reduction ...
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bioRxiv - Developmental Biology 2024Quote: ... Generated cDNA and both types of libraries were evaluated for quantity and quality using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32851) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...