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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the RNA preparation samples were tested by performing standard spectroscopic measurements (Nanodrop One) and using the Qubit 1X dsDNA HS assay kit (Thermo Fisher Scientific). RNA was quantified using the Qubit RNA HS assay kit.
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bioRxiv - Cell Biology 2020Quote: ... The resultant mixture was centrifuged at 14,000 g and the supernatant was used to estimate the total protein by using Pierce™ BCA Protein Assay Kit (Thermo scientific). Equal quantity of protein (300 μg ...
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bioRxiv - Cell Biology 2020Quote: A total of 50 μg of protein from whole-cell or isolated mitochondria were estimated using a Pierce BCA Assay Kit (Thermo Fisher Scientific). Pellets were solubilised in solubilisation buffer (1 % [w/v] SDC ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
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bioRxiv - Cell Biology 2020Quote: ... Membrane pellets were gently resuspended in 100 μl SEM and protein content was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) to normalize total protein concentration across samples ...
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bioRxiv - Cell Biology 2020Quote: ... The protein content of preovulatory follicles from eCG-injected mice is ~6 μg (Pierce BCA protein assay kit, 23225, Thermo Fisher Scientific). The protein content of follicles (~320-400 μm diameter ...
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bioRxiv - Cell Biology 2020Quote: S phase status was monitored by measuring 5-ethynyl-2’-deoxyuridine (EdU) incorporation with the Click-iT™ Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (ThermoFisher, C10632) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... TAG amounts were normalized to the total protein amounts in each sample using a Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific). The data were analyzed using a Student’s t test.
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bioRxiv - Genetics 2021Quote: ... The gDNA sample integrity was controlled on 1% agarose gel and quantified on Nanodrop and Qubit using the Qubit™ dsDNA BR Assay Kit (Invitrogen™). 2 × 2 µg of gDNA were digested in parallel with 50 units of DpnII (NEB #R0543L ...
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bioRxiv - Microbiology 2020Quote: The proteins from cell lysates and isolated mitochondria were analysed by separating 10-40 μg of protein (concentrations determined using Pierce BCA Protein Assay kit, Thermo Fisher Scientific), by SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of the MDA amplified and purified DNA was measured by Qubit dsDNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Random shotgun libraries were constructed using the Nextera XT kit (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The quantity and purity of all RNA samples was checked using a nanodrop spectrophotometer and a Qubit™ RNA HS assay kit (Invitrogen™).
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bioRxiv - Evolutionary Biology 2020Quote: ... purity and integrity of all RNA samples was checked using a nanodrop spectrophotometer and Qubit™ RNA HS assay kit (Invitrogen™), as well an Experion™ bioanalyser with a prokaryotic RNA standard sensitivity analysis kit (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The quantity and quality of the extracted RNA was assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Q10211) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer RNA Kit (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... The quantity and quality of the generated NGS libraries were evaluated using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... cell proliferation assays were carried out using Click-It EdU Cell Proliferation Kit for Imaging Alexa Flour™ 647 (Thermo Fisher Scientific). Larvae were treated with EdU at a final concentration of 10 μM in FSW and let to grow for 2 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 10 µM EdU using a Click-iT EdU Alexa Fluor 488 flow Cytometry Assay Kit (Thermo Fisher, C10425) and processed as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: We quantified the obtained DNA using the Quant-iT™ dsDNA BR Assay Kit (product nr. Q33130; Thermo Fisher Scientific, Waltham, USA) in the Spark® 10M multimode microplate reader (Tecan Group Ltd ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA concentrations were estimated using a fluorimeter and fluorescent DNA-binding dye (Qubit™ dsDNA BR Assay Kit; Thermo Fisher Scientific, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were stored at - 80°C and protein concentrations were further determined using the Pierce™ BCA Protein Assay Kit (23225, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... The concentration of QuantSeq libraries mRNA-seq library was quantified using the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific cat. no. Q32851) on a Qubit 2.0 Fluorometer ...
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bioRxiv - Molecular Biology 2021Quote: ... Removal of residual DNA was confirmed by DNA-specific quantification using a Quant-IT dsDNA Broad Range Assay Kit (Thermo Fisher Scientific). For cDNA synthesis ...
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bioRxiv - Neuroscience 2021Quote: ... The flow-through for each sample was collected and protein was quantified using the Pierce detergent compatible Bradford assay kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: The concentration of RNA in the lysates were measured using the Quant-iT RNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and Infinite M1000 PRO (TECAN ...
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bioRxiv - Molecular Biology 2021Quote: ... the P2 fraction was brought up in 400uL of PBS and protein concentrations were measured using the QuBit Protein Assay Kit (Thermo Fisher, Q33211). Samples were precleared with 15mg of DEPC treated (to inactivate RNAse ...
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bioRxiv - Immunology 2022Quote: ... The quantity of amplicons was measured with the Qubit 4.0 fluorometer using the 1X dsDNA HS Assay Kit (Thermo Fisher Scientific, USA) and the sequencing libraries were prepared using the Nextera DNA Flex Prep kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... The DNA was stored at 4°C overnight to facilitate DNA homogenization and then quantified using a Qubit dsDNA HS Assay Kit (Molecular Probes/Life Technologies). The labeled DNA was stained with an intercalating dye and left to stand at room temperature for at least 2 hrs before loading onto a Bionano Chip.
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bioRxiv - Microbiology 2021Quote: ... The protein concentration in the cell free extracts was determined with a BCA assay kit (Pierce, Thermo Scientific, Rockford, IL, United States). Enzyme assays were prepared as follows ...
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bioRxiv - Genomics 2020Quote: ... RNA specific fluorescent dye is used to quantify extracted RNA using the Qubit RNA XR assay kit and the Qubit Flourometer system (ThermoFisher, MA, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... permeabilised in ice-cold 100% methanol for 10 minutes at -20°C and labelled using an Alexa Fluor™ 647 HCS assay kit (ThermoFisher Scientific). Cells were blocked in 5% normal goat serum and 2% BSA for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... gRNAs were visualized in a 2% agarose stained by ethidium bromide to check for RNA integrity and quantify using Qubit™ RNA BR Assay Kit (ThermoFisher, Q10210). gRNAs targeting tbxta and tyr CDS in zebrafish and rx3 in medaka were in vitro transcribed from PCR containing a pool of different gRNA primers at equal concentration ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We quantified the protein content in the clarified lysates of the damselflies using Pierce™ BCA protein assay kit (Thermo Fisher Scientific). We used the Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was isolated using RNA-Solv® Reagent and reverse-transcribed with TaqMan MicroRNA Reverse Transcription Kit and TaqMan MicroRNA Assays for each miRNA (Applied Biosystems). Real-time PCR was performed in a total volume of 20 µL on a Step One Plus real-time PCR system (Applied Biosystems ...
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bioRxiv - Physiology 2020Quote: ... Protein pellets from each aliquot per sample were pooled and the total protein content were measured using BCA protein Assay kit (ThermoFisher Scientific, Australia). The protein disulphide bonds were reduced with 10 mM dithiothreitol for 30 min at 60 °C and the cysteine residues were alkylated with 20 mM iodoacetamide for 30 min at 37 °C in the dark ...
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bioRxiv - Genomics 2020Quote: ... Treated cells were lysed with CLB1 buffer (Zeptosens, Bayer) and quantified using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific 23227). Samples were diluted (100% ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA quantity measurement of isolated DNA samples took place using a Qubit fluorometer with double stranded DNA (dsDNA) High Sensitivity Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... permeabilization and Click-IT reaction were performed using the Click-iT Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (Fisher Scientific, C10458), according to the manufactureŕs protocol ...
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bioRxiv - Genetics 2020Quote: Total peroxidase activity (POX) was measured with the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit according to manufacturer’s instructions (Invitrogen, Carlsbad, CA). Pulverized leaves were homogenized in 20mM sodium phosphate buffer (pH 6.5 ...
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bioRxiv - Genomics 2021Quote: ... Elution was performed in nuclease-free water and 1 μL was used for measuring the concentration with the Qubit™ dsDNA High Sensitivity Assay Kit (ThermoFisher Scientific), while the size was assessed with a High Sensitivity DNA chip on an 2100 Bioanalyzer.
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bioRxiv - Genetics 2020Quote: ... The concentrations of DNA from blood and saliva samples were determined using the fluorescence-based Quant iT dsDNA Board-Range assay kit (cat # Q-33130, Life Technologies, CA). Fluorescence was measured with a Tecan Infinite M 200 Pro multimode microplate reader (Tecan ...
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bioRxiv - Developmental Biology 2020Quote: ... 1% Sodium Citrate pH6.0 (30 minutes at room temperature): Proteinase K (1/25 of stock solution from the Click-iT™ Plus TUNEL assay kit - Molecular Probes #C10617 for 30 minutes at room temperature) ...
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bioRxiv - Developmental Biology 2020Quote: ... These cells were fixed with 1% PFA overnight and OPP signal was detected using Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen) as the manufacturer′s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and assessed the quality and quantity using Tecan Infinite® 200 NanoQuant and Quant-iT PicoGreen® dsDNA assay kit (ThermoFisher Scientific). We determined the sex of fish following Peichel et al ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quality control of the RNA was performed on agarose gels and the Qubit RNA HS or BR Assay kit (Invitrogen, Carlsbad, CA) for quantification ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein extracts were diluted in PBS and quantified in duplicate using a Micro BCA Protein Assay Kit (ThermoFisher Scientific, cat no: 23235). Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: Annexin V apoptosis and dead cell detection assay was performed with the Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen, cat# V13241) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The concentration of the final pool was measured with the Qubit™ dsDNA HS Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). The final pool was processed to be sequenced on the Miseq and NextSeq platforms (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted using a modified phenol-chloroform method (11, 75) and quantified with the dsDNA High Sensitivity Assay kit (Thermo Fisher Scientific) on a Qubit 3.0 Fluorometer ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% SDS and protease and phosphatase inhibitors) and stored at −20°C before sonication and quantification by a Pierce BCA Protein Assay Kit (Thermofisher Scientific, 23225). Protein lysates were heated for 5 min at 95°C in loading buffer (1% SDS ...