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Citations for Thermo Fisher :
9101 - 9150 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: RNA libraries were prepared from reverse-transcribed cDNA samples on the Ion Chef System using the Ion AmpliSeq Kit for Chef DL8 and Oncomine Immune Response Research Assay (Thermo Fisher Scientific). RNA libraries were immediately used for sequencing ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with O-propargyl-puromycin (OPP) using Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit (Invitrogen) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of SAGs were transferred to replica plates for DNA yield quantification using Quant-iT dsDNA Broad-Range (BR) Assay Kit (Thermo Fisher Scientific) prior to library preparation ...
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bioRxiv - Microbiology 2023Quote: ... Then each sample was diluted in a 1:10 ratio and measured by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Mitochondria enriched fractions were processed similar to whole cell samples except they were normalized via protein concentration as determined by the Pierce™ BCA Protein Assay kit (Thermo Scientific). In Brief ...
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bioRxiv - Biochemistry 2022Quote: ... and supernatants were sampled and diluted 30-fold for calculation of the protein concentration using the Coomassie Plus Staining Reagent (Bradford) Assay Kit (Thermo Fisher Scientific). Cell lysates were normalised for total protein concentration and processed for immunoblotting ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting cDNA was evaluated for quantity and quality using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32851) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: ... EdU staining was conducted according to the manufacturer’s instructions using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10425). Attune NxT flow cytometry was used for analysis of cells (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... and supernatants were sampled and diluted 30-fold to calculate protein concentration using the Coomassie Plus Staining Reagent (Bradford) Assay Kit (Thermo Fisher Scientific). Cell lysates were then normalised and processed for immunoblotting.
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bioRxiv - Bioengineering 2022Quote: ... samples were centrifuged at 3000g for 10 minutes and the DNA concentration was measured by Qubit dsDNA HS assay kit (Life Technologies, Q32851). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein levels in the samples were assayed using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific; Cat. # 23227) and diluted with 500 mM TEAB so that the total protein level of each sample would be approximately the same ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein concentration in the supernatant was measured using the Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Waltham, MA, USA). After diluting with loading buffer and boiling for 10 min at 95°C ...
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bioRxiv - Cell Biology 2022Quote: ... supernatant was used for analysis with GLP-1, Active form Assay Kit (Immuno-Biological Laboratories, Gunma, Japan) and microplate reader (Varioskan LUX, Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... The total protein concentration for each culture was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Using the same amount of protein from each sample ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified from yeast supernatants by Ni-NTA resin affinity chromatography and protein concentration was determined using Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... were washed according to the PacBio protocol “Guidelines for using AMPure XP Beads for HiFi sequencing.” These washed beads were used to clean and concentrate sheared DNA with a 0.5X v/v ratio followed by a concentration measurement using the Qubit Fluorometer and the Qubit dsDNA High Sensitivity Assay kit (Invitrogen, Carlsbad, CA). The fragment size distribution was verified on the Femto Pulse System using the Genomic DNA 165 kb Kit.
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bioRxiv - Genetics 2022Quote: ... genomic DNA (gDNA) used in this study was assessed using the Qubit Fluorometer and the Qubit dsDNA Broad Range Assay kit (Invitrogen, Carlsbad, CA). The integrity of the HMW gDNA was verified on the Femto Pulse system using the Genomic DNA 165 kb Kit (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and final RNA concentrations were determined with an Invitrogen™ Qubit™ 2.0 Fluorometer and RNA HS assay kit (Thermo Fisher Scientific). RNA samples were submitted to the University of California San Diego Institute for Genomic Medicine Genomics Center for preparation of mRNA stranded libraries for 100 base pair paired-end RNA-seq ...
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bioRxiv - Genomics 2022Quote: RNA-seq libraries were quantified using a Qubit® fluorometer and Qubit® dsDNA HS Assay Kit (Invitrogen™/Thermo Fisher Scientific), while library quality checks were performed using an Agilent 2100 Bioanalyzer and High Sensitivity DNA Kit (Agilent Technologies Ltd.) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using the PierceTM BCA Protein Assay Kit (Cat# 23225, Pierce Biotechnology, Thermo Fisher Scientific Inc., Waltham, MA, USA). Proteins ...
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bioRxiv - Microbiology 2022Quote: ... The DNA concentration was determined using NanoDrop Spectrophotometer ND-1000 or QubitTM dsDNA Assay kit (Invitrogen Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Pathology 2022Quote: ... The resultant DNA samples were quantified using the Qubit dsDNA BR Assay kit and Qubit 4.0 fluorometer (Life Technologies Corporation, Eugene, OR).
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bioRxiv - Neuroscience 2022Quote: ... RNA concentration was estimated using either Nanodrop (73% of samples) or Qubit RNA HS assay kit (27% of samples) on a Qubit Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The above procedure yielded between 0.13 and 550 ng/µL purified DNA from the vaginal swabs as measured by a Qubit 3.0 fluorimeter and Qubit dsDNA assay kit (Life Technologies, Carlsbad, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenates were centrifuged at 4 °C for 30 min at 9300 × g and total protein concentration in the supernatants was determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... The two overlapping amplicon pools for each specimen were combined and quantified using the Qubit dsDNA HS Assay Kit (Invitrogen; Carlsbad, CA). A total of 75 – 150 ng of PCR product per specimen was barcoded using the Nanopore Rapid Barcoding Kits SQK-RBK004 or SQK-RBK110.96 (Oxford Nanopore Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... HM and IF pooled EV fractions were assayed for total protein according to manufacturer’s instructions (Micro BCA protein assay kit, Thermofisher Scientific, Brisbane, Australia) and a volume equivalent to 20 µg total protein was aliquoted for western blot (WB ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were performed on a 100-μl scale in a 96-well plate according to the “Amplex™ Red Cholesterol Assay Kit” protocol (ThermoFisher Scientific). No cholesterol esterase was added ...
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bioRxiv - Neuroscience 2022Quote: Total protein content in the PBS-soluble (clarified 10% brain homogenate) and detergent-soluble fractions was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) following the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... samples were centrifuged at 3000g for 10 minutes and the DNA concentration was measured by Qubit dsDNA HS assay kit (Life Technologies, Q32851). Briefly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products were purified with AMPure XP beads and then were validated using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) for DNA concentration ...
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bioRxiv - Cell Biology 2022Quote: ... MSC-EVs were not lysed in RIPA buffer and protein content was determined via the Micro BCA™ Protein Assay Kit (ThermoFIsher, 23235).
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bioRxiv - Cancer Biology 2023Quote: ... spun at 13,000 rpm for 30 minutes at 4°C and supernatant protein concentration determined using the BCA assay kit (#23225; Thermo Fisher Scientific). 30-50 μg aliquots of lysate proteins were resolved on sodium dodecyl sulfate-7.5% polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Genetics 2022Quote: ... A protein concentration of the tissue lysates was determined with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and 3-10 µg of the total protein lysate/homogenate used per assay well in duplicates and repeated in three independent runs ...
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bioRxiv - Developmental Biology 2023Quote: OPP staining was performed using a Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit (ThermoFisher Scientific; Cat. No. C10456). Mouse embryos were first cultured in vitro from E1.5 to E2.5+7h in KSOM+AA as described above ...
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bioRxiv - Genomics 2023Quote: ... at a 1.6:1 volumetric bead-to-DNA ratio and quantification using the dsDNA High Sensitivity Assay kit on a Qubit® fluorometer 3.0 (Thermo Fisher Scientific), samples were diluted to 0.5 nM in a total of 24 μl and used as input for library preparation following the PCR barcoding (96 ...
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bioRxiv - Genomics 2023Quote: All PCR products were assessed by gel electrophoresis and quantified using the Qubit™ dsDNA HS Assay kit (Cat. #Q32854; Thermo-Fisher Scientific). Primers were synthesized by Integrated DNA Technologies® and diluted to 10uM in 0.1x TE buffer ...
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bioRxiv - Genomics 2023Quote: ... The DNA purity of samples was evaluated with a NanoDrop spectrophotometer and the concentrations were quantified using Qubit dsDNA HS Assay Kit with Qubit™ 4.0 (Invitrogen, CA, USA).
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bioRxiv - Genomics 2023Quote: ... Library quality control was performed by measuring concentration with Qubit® dsDNA HS Assay Kit (Thermo Fisher Scientific Inc., Cat. No. Q32854) and fragment size distribution with the Agilent® High Sensitivity DNA Kit (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... DNA concentration (minimum of 80 ng/µL) was measured using Qubit DNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA). For PacBio sequencing ...
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bioRxiv - Plant Biology 2023Quote: High molecular weight genomic DNA was extracted from young leaves of greenhouse-grown plants using the CTAB method,34 and concentration determined by Qubit dsDNA HS assay kit (Invitrogen, Waltham, MA). For each of the samples ...
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bioRxiv - Immunology 2023Quote: ... Click Chemistry labelling was performed according to Click-iT™ Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (ThermoFisher, cat: C10458). The percentages of mitochondrial dependence ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... protein concentration was standardised to 20 μg/mL according to the protein quantification results tested by the Micro BCA protein assay kit (Thermo Fisher Scientific). Reduction was carried out using 10 μL TCEP was added followed by incubation at 37 °C for 45 min followed by alkylation with 55 mM iodoacetamide ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenates were cleared by centrifugation at 13,000 x g at 4°C for 25 min and the protein concentration in the collected supernatant was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Protein extracts (20 µg of protein for each sample) ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations from PBS-soluble (soluble) and sarkosyl-soluble (insoluble) fractions were assessed using the BCA method (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific). Total protein lysates from PBS-soluble fractions and sarkosyl-soluble fractions (50µg ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were then centrifuged at 10,000 rpm for 10’ at 4°C and the supernatant was collected to determine protein concentration using Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Before loading ...
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bioRxiv - Plant Biology 2023Quote: ... The quality and quantity of the purified cDNA were determined using a Qubit 3 Fluorometer with a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and an Agilent 2100 BioAnalyzer with a high-sensitivity DNA chip (Agilent Technologies) ...
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bioRxiv - Genomics 2023Quote: ... and DNA concentration was measured using a Qubit® DNA Assay Kit in a Qubit® 4.0 Fluorometer (Life Technologies, CA, USA). DNA integrity was checked by pulsed field gel electrophoresis (PFGE) ...