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Citations for Thermo Fisher :
9001 - 9050 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Relative RNA values were normalised against the concentration of the purified cDNA as measured by Qubit dsDNA High Sensitivity Assay kit (Thermo Fisher Scientific). This method of normalization was treated as an additional step because the DAC treatments can lead to decrease of RNA yield and changes in housekeeping gene expression ...
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bioRxiv - Immunology 2021Quote: ... was performed using the Click-iT Plus EdU A488 or the Click-iT Plus Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific) following manufacturer’s instructions with minor modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of the DNA fragments was quantified by using a fluorescence dye and a fluorometer (Qubit dsDNA BR assay kit; Thermo Fisher Scientific). The amplified DNA fragments (100 ng ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentrations were quantified using the QubitTM 1.0 Fluorometer and the Qubit RNA BR Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). RNA purity and integrity were evaluated using a Bioanalyzer RNA 6000 Nano chip (Agilent Technologies ...
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bioRxiv - Immunology 2020Quote: ... One μL of the diluted libraries were used to quantify as recommended in the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) with standard range from 0 to 14 ng/ well ...
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bioRxiv - Immunology 2020Quote: ... quality control was performed in order to evaluate 1) the concentration of each sample using the Qubit dsDNA high sensitivity assay kit (Life Technologies, Q32854), and 2 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell debris were removed by centrifugation at 1000 × g for 5 min at 4°C and the protein concentration of the resulting supernatant was estimated using the BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as the standard ...
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bioRxiv - Immunology 2021Quote: ... This was done following the “Click-iT EdU Flow Cytometry Assay Kit protocol” (Molecular probes by Life technologies, catalog numbers C10419, C10420). Cells were then analyzed by flow cytometry using the BD LSR FORTESSA and data analysis was done using the
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bioRxiv - Genomics 2021Quote: ... Munich/Germany) and DNA quantity using a Qubit dsDNA BR assay kit in combination with a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA/USA). The DNA of all samples was pooled for the sequencing library ...
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bioRxiv - Neuroscience 2021Quote: ... Levels of murine Aβ (1–42) were measured by enzyme-linked immunosorbent assay (ELISA) using commercial kits (Thermo Fisher Scientific, cat# KMB3441) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... RNA concentration was measured using Qubit High Sensitivity RNA Assay Kit and Qubit 3.0 Fluorometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). The integrity of total RNA was assessed on the Agilent Fragment Analyzer 5200 system (Agilent Technologies ...
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bioRxiv - Genomics 2021Quote: ... The quality and quantity of the gDNA was estimated using a Nanodrop spectrophotometer and a Qubit fluorometer using the Qubit™ dsDNA BR assay kit (#Q32850) from Life Technologies, respectively.
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bioRxiv - Genomics 2021Quote: ... The concentrations of the libraries were measured on a Qubit 4 fluorometer using the ds DNA HS Assay Kit (Thermo Fisher Scientific). The shape and the mean fragment size of the libraries were determined on a 5200 Fragment Analyzer using the HS NGS Fragment Kit 1-6000 bp (both Agilent) ...
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bioRxiv - Cell Biology 2021Quote: To assess the amounts of H2O2 excreted by ESCs into the extracellular space Amplex™ Red Hydrogen Peroxide/Peroxidase Assay Kit (Invitrogen, USA) was used according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Mitochondria enriched fractions were processed similar to whole cell samples except they were normalized via protein concentration as determined by the Pierce™ BCA Protein Assay kit (Thermo Scientific). In Brief ...
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bioRxiv - Biochemistry 2022Quote: ... and supernatants were sampled and diluted 30-fold for calculation of the protein concentration using the Coomassie Plus Staining Reagent (Bradford) Assay Kit (Thermo Fisher Scientific). Cell lysates were normalised for total protein concentration and processed for immunoblotting ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting cDNA was evaluated for quantity and quality using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32851) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: ... EdU staining was conducted according to the manufacturer’s instructions using the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Thermo Fisher Scientific, C10425). Attune NxT flow cytometry was used for analysis of cells (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... and supernatants were sampled and diluted 30-fold to calculate protein concentration using the Coomassie Plus Staining Reagent (Bradford) Assay Kit (Thermo Fisher Scientific). Cell lysates were then normalised and processed for immunoblotting.
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bioRxiv - Bioengineering 2022Quote: ... samples were centrifuged at 3000g for 10 minutes and the DNA concentration was measured by Qubit dsDNA HS assay kit (Life Technologies, Q32851). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... The total protein levels in the samples were assayed using the Pierce™ BCA Protein Assay kit (Thermo Fisher Scientific; Cat. # 23227) and diluted with 500 mM TEAB so that the total protein level of each sample would be approximately the same ...
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bioRxiv - Developmental Biology 2022Quote: ... the protein concentration in the supernatant was measured using the Pierce BCA Protein Assay Kit (23225, Thermo Fisher Scientific, Waltham, MA, USA). After diluting with loading buffer and boiling for 10 min at 95°C ...
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bioRxiv - Cell Biology 2022Quote: ... supernatant was used for analysis with GLP-1, Active form Assay Kit (Immuno-Biological Laboratories, Gunma, Japan) and microplate reader (Varioskan LUX, Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... The total protein concentration for each culture was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Using the same amount of protein from each sample ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified from yeast supernatants by Ni-NTA resin affinity chromatography and protein concentration was determined using Coomassie (Bradford) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... were washed according to the PacBio protocol “Guidelines for using AMPure XP Beads for HiFi sequencing.” These washed beads were used to clean and concentrate sheared DNA with a 0.5X v/v ratio followed by a concentration measurement using the Qubit Fluorometer and the Qubit dsDNA High Sensitivity Assay kit (Invitrogen, Carlsbad, CA). The fragment size distribution was verified on the Femto Pulse System using the Genomic DNA 165 kb Kit.
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bioRxiv - Genetics 2022Quote: ... genomic DNA (gDNA) used in this study was assessed using the Qubit Fluorometer and the Qubit dsDNA Broad Range Assay kit (Invitrogen, Carlsbad, CA). The integrity of the HMW gDNA was verified on the Femto Pulse system using the Genomic DNA 165 kb Kit (Agilent Technologies ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and final RNA concentrations were determined with an Invitrogen™ Qubit™ 2.0 Fluorometer and RNA HS assay kit (Thermo Fisher Scientific). RNA samples were submitted to the University of California San Diego Institute for Genomic Medicine Genomics Center for preparation of mRNA stranded libraries for 100 base pair paired-end RNA-seq ...
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bioRxiv - Genomics 2022Quote: RNA-seq libraries were quantified using a Qubit® fluorometer and Qubit® dsDNA HS Assay Kit (Invitrogen™/Thermo Fisher Scientific), while library quality checks were performed using an Agilent 2100 Bioanalyzer and High Sensitivity DNA Kit (Agilent Technologies Ltd.) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were estimated using the PierceTM BCA Protein Assay Kit (Cat# 23225, Pierce Biotechnology, Thermo Fisher Scientific Inc., Waltham, MA, USA). Proteins ...
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bioRxiv - Microbiology 2022Quote: ... The DNA concentration was determined using NanoDrop Spectrophotometer ND-1000 or QubitTM dsDNA Assay kit (Invitrogen Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Pathology 2022Quote: ... The resultant DNA samples were quantified using the Qubit dsDNA BR Assay kit and Qubit 4.0 fluorometer (Life Technologies Corporation, Eugene, OR).
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bioRxiv - Neuroscience 2022Quote: ... RNA concentration was estimated using either Nanodrop (73% of samples) or Qubit RNA HS assay kit (27% of samples) on a Qubit Fluorometer (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... The above procedure yielded between 0.13 and 550 ng/µL purified DNA from the vaginal swabs as measured by a Qubit 3.0 fluorimeter and Qubit dsDNA assay kit (Life Technologies, Carlsbad, CA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The homogenates were centrifuged at 4 °C for 30 min at 9300 × g and total protein concentration in the supernatants was determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Microbiology 2022Quote: ... The two overlapping amplicon pools for each specimen were combined and quantified using the Qubit dsDNA HS Assay Kit (Invitrogen; Carlsbad, CA). A total of 75 – 150 ng of PCR product per specimen was barcoded using the Nanopore Rapid Barcoding Kits SQK-RBK004 or SQK-RBK110.96 (Oxford Nanopore Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... and 1X concentration OPP reaction buffer (the last two reagents derived from the Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen) and at concentrations relative to those recommended in the manufacturer’s instructions) ...
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bioRxiv - Physiology 2022Quote: ... The protein concentration of the samples was adjusted to equal amounts using Pierce BCA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) for subsequent immunoprecipitation ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined by bicinchoninic acid method (Smith et al., 1985) using the Pierce™ BCA Protein Assay kit (Thermo-Fisher Scientific). Total protein lysates (30 µg ...
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bioRxiv - Molecular Biology 2022Quote: ... The isolated DNA was quantified by fluorometry using the Quant-iT high sensitivity dsDNA assay kit on the Qubit fluorometer (Invitrogen; Thermo Fisher Scientific). The DNA samples were stored at -20 °C until further molecular analyses.
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bioRxiv - Microbiology 2022Quote: ... The quantity and quality of the extracted DNA were assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854) and an Agilent Femto Pulse system with an Ultra Sensitivity NGS kit (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The quantity and quality of the cleaned amplicons were assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854) and an Agilent Fragment Analyzer (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: Pooled samples were quantified with a Qubit 1X dsDNA HS Assay Kit on a Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). Single-read sequencing was performed on a NovaSeq 6000 device using an S2 Reagent kit (100 cycles ...
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bioRxiv - Immunology 2022Quote: mRNA expression of type 2 cytokines in ILC2s was measured by using PrimeFlow™ RNA Assay Kit (ThermoFisher Scientific # 88-18005-210) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Sub-placenta/decidua tissue was homogenized in ice-cold RIPA buffer containing protease and phosphatase inhibitors and protein concentrations determined using the Pierce™ Coomassie Blue Assay kit (Thermo Scientific) following standard protocol ...
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bioRxiv - Physiology 2022Quote: ... placenta tissue was homogenized in ice-cold RIPA buffer containing protease and phosphatase inhibitors and protein concentrations determined using Pierce™ Coomassie Plus Assay Kit (Thermo Scientific) following standard protocol ...
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bioRxiv - Microbiology 2022Quote: ... DNA quality was evaluated visually via gel electrophoresis and quantified using a Qubit 3.0 fluorometer (Thermo-Fisher, Waltham, MA, USA) and Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Waltham, MA, USA) for TBC and RUMC samples ...
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bioRxiv - Neuroscience 2022Quote: ... Plates were read using an MSD SECTOR Imager 2400 and resulting peptide concentrations were normalized to total protein in the cell lysate per well measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Data for each shRNA knockdown were additionally normalized to the average of control conditions for each parameter measured.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The pellet was resuspended in PBS/EDTA buffer and the protein concentration of the homogenate measured using the Pierce BCA protein assay kit (Thermo Scientific, USA). AChE activity was measured using the Ellman assay using acetylthiocholine as substrate and the indicator 5,5′-dithiobis-2-nitrobenzoic acid (DTNB ...