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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Intensity quantification was performed using ImageJ software and the obtained values were corrected by culture media background (without having been in contact with cells) and total protein content measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). Values were further normalized by internal negative and positive controls from the antibody assay ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined following centrifugation of the lysate at 16,000 x g at 4 °C for 20 minutes using the using a Pierce™ Coomassie (Bradford) Protein Assay Kit (23200, Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were centrifuged at 14000 rpm for 15 minutes at 4°C and protein concentration determined with a Pierce™ BCA Protein Assay Kit (Life Technologies). High capacity neutravidin beads (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Living cell numbers and metabolic activity were measured with an MTT assay or labeled for 1 h using the LIVE/DEAD® kit (ThermoFisher Scientific) containing 2 μM calcein and 4 μM EthD-1 (Ethidium homodimer-1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear membrane and debris were pelleted by centrifugation and protein concentration of the cleared lysate was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). 2μg of the anti-BAF155 antibody (ab172638 ...
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bioRxiv - Cancer Biology 2020Quote: The relative release of glutamate from cells into the extracellular medium was detected using an Amplex Red glutamate release assay kit (Thermo Fisher Scientific). Glutamate release was first normalized to the total cell number determined with the CCK8 kit at the end of the experiment ...
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bioRxiv - Biochemistry 2020Quote: ... The cell debris was removed by centrifugation and the protein concentration of the supernatants were determined using Pierce™ bicinchoninic acid (BCA) protein assay kit (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The 300 μl collected was aliquoted into an individual well in a 96-well microplate containing 100 μl each (3 wells total for a single well in the 24-well plate) and subjected to a CyQuant© Direct Proliferation Assay Kit (Thermo Fisher) per the manufacturers protocol ...
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bioRxiv - Microbiology 2021Quote: ... Concentrations of the amplified gene fragments were determined by a Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen, Waltham, MA, USA) and used for standard curve ...
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bioRxiv - Microbiology 2021Quote: ... whereas the DNA-removing efficacy was evaluated using the Qubit 1× dsDNA HS assay kit (Thermo Fisher Scientific, Waltham, MA, United States).
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with 10 µM EdU for 3 h and analyzed using Click-iTTM EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific). Cells were recorded with a BDTM LSRII (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... Libraries were quantified using the IS7 and IS8 primers65 in a quantification assay using a DyNAmo SYBP Green qPCR Kit (Thermo Fisher Scientific) on the LightCycler 480 (Roche) ...
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bioRxiv - Microbiology 2021Quote: The release of Pi by pyrophosphatase activity of VP1 proteins in isolated yeast vesicles was measured using the PiPer ™ Phosphatase Assay Kit (P22061, ThermoFisher Scientific), according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... followed by collection of supernatants for quantification of total protein concentration using Microplate BCA Protein Assay Kit (Pierce™, Thermo Fisher Scientific). Equal amounts of protein were separated by SDS-PAGE using 4-15% SDS polyacrylamide gels (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: The enumeration of apoptotic cells was performed using the CellEventTM Caspase-3/7 Green Flow Cytometry Assay Kit (catalogue #: C10427; Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... The quantity of the sequencing-ready library was measured using Qubit dsDNA HS assay kit (Cat. No. Q32854, Invitrogen, Carlsbad, California, USA) and quality was checked by Agilent DNA HS kit (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA yield was measured right after IVT using the Qubit RNA Broad Range Assay kit with the Qubit Fluorometer (Thermo Fisher, UK) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... DNA quantification in the spent medium of the donor was performed through fluorescent labelling with Quant-iT reagent (Invitrogen Qubit dsDNA HS Assay Kit, Thermo Fisher Scientific). Fluorescence was measured with Infinite M200 PRO (TECAN ...
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bioRxiv - Plant Biology 2021Quote: ... RNA concentration and quality were assessed by fluorometry and electrophoresis using a Qubit™ 4 and Qubit RNA HS Assay Kit (ThermoFisher Scientific) and a TapeStation (Agilent).
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bioRxiv - Plant Biology 2020Quote: ... RNA quality and concentration were assessed using a NanoDrop™ 1000 spectrophotometer and a Qubit 4 Fluorometer with an RNA HS Assay Kit (ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Purified DNA from each sample was first quantified using a Qubit 3.0 fluorometer with a Qubit dsDNA Assay kit (Life Technologies, Carlsbad, CA). For the inhibition test ...
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bioRxiv - Molecular Biology 2020Quote: ... The biotinylated DNA fragments were applied to the gel-shift assay by using the LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, US) and Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was then carried out using the corresponding TaqMan™ Gene Expression Assay Kit that include exonspanning probes for genes of interest (ThermoFisher Scientific) (product numbers listed in Supplemental Table 5 ...
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bioRxiv - Biochemistry 2021Quote: ... and an equal amount of protein was used for labeling by Alexa Fluor 488 using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (ThermoFisher). Labeled proteins were resolved on 12% SDS gel and visualized on a ChemiDoc Imager (BioRad) ...
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bioRxiv - Cancer Biology 2020Quote: Cell cycle analysis was performed using the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow cytometry assay kit (Thermofisher Scientific). To obtain the cell cycle profile ...
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bioRxiv - Cell Biology 2020Quote: ... was solubilized with 0.1 N NaOH and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA). Cellular cholesterol content data was expressed as mg cholesterol/mg of protein ...
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bioRxiv - Cell Biology 2020Quote: ... Here after we followed the instructions provided by the manufacturer (PrimeFlow™ RNA assay kit, Cat no 88-18005-210, eBioscience/Thermo Fisher). In brief the samples were incubated with the first fixation buffer (PrimeFlow fixation buffer 1 ...
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bioRxiv - Biophysics 2020Quote: ... For more accurate characterization protein concentration was determined by the bicinchoninic acid method31 with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). A method based on pyridine hemochrome spectra32 was used to determine the amount of heme ...
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bioRxiv - Biochemistry 2022Quote: ... The genomic DNA concentration was determined using the Qubit dsDNA BR Assay Kit and Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cell proliferation was measured using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Thermo Fisher, C10632). Cells were treated with 10 μM EdU (5-ethynyl-2’-deoxyuridine ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration of each lysate was measured using the BCA protein assay kit according to manufacturer’s directions (Thermo-Fisher Scientific, Waltham, MA). Proteins were separated on a 10% SDS-PAGE gel with equivalent amounts of protein loaded in each lane (Thermo-Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All quantification steps of gDNA and NGS libraries were done with Qubit dsDNA HS Assay Kit (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA).
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bioRxiv - Genetics 2022Quote: ... ddPCR amplification of the cDNA was performed on 1.33 μL of each cDNA dilution combined with 1 μL of miRNA-specific 20X TaqMan MicroRNA Reagent containing probes and primers for amplification from the TaqMan MicroRNA Assay Kit (Thermo Fisher Scientific), 10 μL of 2X ddPCR Supermix for probes (no dUTP ...
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bioRxiv - Molecular Biology 2022Quote: ... The genomic DNA concentration was measured by fluorimetry (Turner Biosystems) using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher). sgRNA sequences were amplified by PCR with the NEBNext High Fidelity PCR Master mix using barcoded primers as described previously (Yau and Rana ...
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bioRxiv - Microbiology 2022Quote: Peptide concentrations were measured for fraction two of each sample using the Quantitative Fluorometric Peptide Assay kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2022Quote: ... The quality and size of the precursor was checked on agarose gel and its concentration was measured using Qubit RNA BR (Broad Range) Assay Kit with the Qubit Fluorometer (Thermo Fisher Scientific). The concentration ranged from 1500 ng/μl to 2000 ng/μl.
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined using the Thermo Scientific™ Pierce™ BCA (bicinchoninic acid) Protein Assay Kit (Thermo Scientific™ Cat# 23225). Following concentration determination ...
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bioRxiv - Microbiology 2022Quote: ... then samples were stored at -80°C until measuring Gaussia luciferase activity using the Gaussia Luciferase Flash Assay Kit (ThermoFisher Scientific, 16158) as per the manufacturer’s directions on a FLUOstar Omega microplate reader (BMG Labtech) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The quality of the RNAs used to prepare RNA-seq libraries was checked with the Qubit RNA BR Assay Kit (Thermo Fisher, USA). SMAT libraries ...
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bioRxiv - Immunology 2022Quote: ... The final antibody concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Purified antibodies were stored at -80°C prior to use.
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bioRxiv - Neuroscience 2020Quote: ... Lysates were passed through a 0.22 μm Spin-X filter column after which total protein amount was determine using the Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Equal protein amounts were denatured in SB and subjected to SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations in the tissue lysates were measured with the aid of a bicinchoninic acid protein assay kit (Thermo Scientific, Rockford, IL). TNF-α ...
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bioRxiv - Bioengineering 2021Quote: ... Total protein quantification was performed in samples using Pierce™ BCA (Bicinchoninic Acid) Protein Assay Kit (Thermo Fisher Scientific Inc. Waltham, MA) following manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... sonicated 3 three times for 10 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... Library concentration was assessed with a BioTek Gen5 Wellplate reader with the Quant-iT PicoGreen dsDNA Assay kit (Thermo Fisher; CAT # P11496), and analyzed on the Bioanalyzer to ensure appropriate size distributions and rule out adaptor contamination.
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bioRxiv - Neuroscience 2021Quote: ... The library quality was checked using an Agilent 2100 Bioanalyzer and concentration was measured by a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and adjusted to 2nM before sequencing (single end ...
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bioRxiv - Plant Biology 2021Quote: Total proteins were extracted from 100 mg of foliar tissues with the IP buffer and quantified with a Pierce BCA protein assay kit (Thermo Fisher Scientific). Afterward ...
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bioRxiv - Neuroscience 2021Quote: ... the cleared supernatant was collected and the protein content in the soluble fraction was determined using the Pierce® BCA Protein Assay Kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The barcode sequencing libraries were quantified using a Qubit 2.0 with a Qubit™ dsDNA HS Assay Kit (Invitrogen P/N Q32854) and the quality of the libraries were performed on a 2100 Bioanalyzer from Agilent using an Agilent High Sensitivity DNA kit (Agilent P/N 5067-4626) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA concentration was assessed using the Qubit ds DNA HS Assay Kit in a Qubit Fluorometer 3.0 (Thermo Fisher Scientific, USA). Whole genome sequencing of clones was performed via 2×250 bp paired end sequencing (Nextera XT sample ...