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Citations for Thermo Fisher :
9201 - 9250 of 10000+ citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... cDNA library molarity and quality were assessed using the Qubit 4.0 with the Qubit™ 1X dsDNA HS Assay Kit (ref. Q33231; Thermo Fisher Scientific) and the TapeStation with the Agilent D1000 ScreenTape (ref ...
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bioRxiv - Neuroscience 2024Quote: ... Aliquots (5□μl) of the homogenate were used for protein determination using a BCA assay kit (Pierce) (Lot# RG235624; Thermo Fisher Scientific). Equal amounts of striatal lysates (20□μg ...
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bioRxiv - Molecular Biology 2024Quote: ... We quantify mouse and human motor neuron cell viability with the LIVE/DEAD™ Viability/Cytotoxicity Assay Kit (Thermo Fisher Scientific, #L32250). The neuronal media was replaced by a fresh working solution containing 0.25 μM Deep Red Nucleic Acid Stain and 2 μM Calcein Amin in neuronal imaging media (Hibernate A Low Fluorescence ...
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bioRxiv - Pathology 2024Quote: RNA concentration was measured with the Qubit RNA HS Assay Kit on the Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Immunoprecipitated DNA was purified using the Qiagen MiniElute PCR purification kit before quantification using the Qubit dsDNA High Sensitivity assay (Thermo Fisher Scientific). Enrichment of DNA encoding known MLL1 and Menin binding sites were validated using ChIP-qPCR prior to library preparation using the Takara DNA-Thruplex Library Prep kit ...
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bioRxiv - Genomics 2024Quote: ... gDNA was quantified using a Qubit 2.0 fluorometer and dsDNA BR (double-stranded DNA, broad-range) assay kit (Life Technologies, Carlsbad, CA). Quality control of extracted gDNA samples prior to library preparation was performed using the TapeStation 2200 system (Agilent ...
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bioRxiv - Genomics 2024Quote: ... and the concentration of each sample determined via a Qubit fluorometer with the QubitTM RNA Broadrange (BR) assay kit (Thermo Fisher Scientific). Samples corresponding to five replicates of each of the parental lines and the VIR and AVR F1 pools were used to generate a total of 20 Illumina TruSeq stranded mRNA-Seq libraries which were sequenced in 150 bp paired-end mode on an Illumina HiSeq4000 at Edinburgh Genomics.
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bioRxiv - Genetics 2024Quote: ... Skin tissue was lysed using RIPA buffer and the obtained lysates were quantified for the protein concentration using BCA Protein Assay Kit (Thermo Scientific, USA). 30 mg of proteins were resolved on 10% SDS-PAGE and transferred onto the PVDF membrane ...
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bioRxiv - Synthetic Biology 2024Quote: ... The encapsulation efficiency (EE) of IVT mRNA within PoLixNano was assessed using a Quant-iTTM RiboGreen RNA Assay kit (R11490, Thermo Fisher Scientific). PoLixNano samples were dispersed in 1 ×Tris-EDTA (TE ...
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bioRxiv - Genomics 2024Quote: ... The biotinylated DNA was then purified once again with 1× AMPure XP beads and quantified using a Qubit dsDNA HS Assay kit (ThermoFisher, Cat.N: Q32851).
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bioRxiv - Cancer Biology 2024Quote: All generated libraries were evaluated for quantity and quality using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32851) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...
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bioRxiv - Systems Biology 2024Quote: ... Total RNA samples were quantitatively and qualitatively assessed using the fluorescence-based Broad Range Quant-iT RNA Assay Kit (Thermo Fisher Scientific) and the Standard Sensitivity RNA Analysis DNF-471 Kit on a 96-channel Fragment Analyzer (Agilent) ...
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bioRxiv - Genomics 2024Quote: ... The concentration of the extracted gDNA samples was examined using Qubit dsDNA BR kit fluorometric assay on Qubit 4.0 Fluorometer (Thermo Fisher Scientific, USA), and the fragment size was assessed using gDNA 165 Kb kit on a Femto Pulse System (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... The concentration of total RNA was measured using a Qubit RNA Assay Kit on a Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, California). RNA sequencing libraries with 250–350 bp insert sizes were prepared using Kapa RiboErase (Roche ...
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation by tracking new DNA synthesis was performed with Alexa Fluor 647™ Click-iT® Assays kit (Thermo Scientific™) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The amount of protein in protein corona-coated LNP-168-Luc was determined using a BCA assay kit (Thermo Fisher Scientific, 23225). The obtained protein samples were further lyophilized and stored at -20°C for further experiment.
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bioRxiv - Cancer Biology 2024Quote: Cell proliferation in response to GNE-3511 treatment was assessed by 5-ethynyl-2′-deoxyuridine (EdU) incorporation using the Click-iT EdU Alexa Fluor 488 cell proliferation assay kit (Invitrogen, Carlsbad, CA). CAL33 cells were seeded in µ-Plate 24 Well plates (Ibidi GmbH ...
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bioRxiv - Cell Biology 2024Quote: ... The total protein concentration in each cell lysate was measured using the PierceTM bicinchoninic acid (BCA) Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: MOLM-13 cells were collected and lysed (Cell lysis buffer, Beyotime) as our previously described.7 Equal amounts of protein (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific) were separated by 10% SDS-PAGE gel ...
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bioRxiv - Cancer Biology 2024Quote: ... Dried sample extracts were resuspended in MeOH:H2O (4:1, v/v) according to the total protein content evaluated using BCA Protein Assay Kit (Thermo Scientific, Masschusetts, US) Cell extracts were then analyzed by Hydrophilic Interaction Liquid Chromatography coupled to tandem mass spectrometry (HILIC - MS/MS ...
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bioRxiv - Biochemistry 2024Quote: ... The pellet was resuspended in lysis buffer and total protein content was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of protein from each sample were mixed with 4X Laemmli sample buffer containing 10% β-mercaptoethanol and separated on a 12% SDS-PAGE gel ...
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bioRxiv - Cell Biology 2024Quote: ... Protein assays were performed to quantify purified proteins by the EZQ™ Protein Quantification Kit (Thermo Fisher Scientific, San Jose, CA, USA) with the SoftMax Pro Software v5.3 under the SpectraMax M5 (Molecular Devices ...
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bioRxiv - Cancer Biology 2024Quote: Cell cycle analysis was performed using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (cat. C10424; Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: TMB measurement was performed on 20 ng of genomic DNA with the Oncomine™ Tumor Mutation Load Assay kit (Thermo Fisher Scientific). Quantification of the libraries was carried out using Ion Library TaqMan™ Quantification kit in the QuantStudio 5 real-time quantitative PCR instrument ...
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bioRxiv - Cancer Biology 2024Quote: ... Lactate dehydrogenase (LDH) release in the organoid culture supernatant was measured after 48 hours using the CyQUANT™ LDH Cytotoxicity Assay Kit (Thermo Fisher).
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA concentration and DNA fragment length were assessed using the Qubit dsDNA BR Assay kit on the Qubit Fluorometer (Thermo Fisher Scientific) and the Genomic DNA Screen Tape on the Agilent 2200 (Agilent Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... The concentration of RNA-seq libraries was determined with a Qubit high sensitivity dsDNA HS assay kit (Thermo Fisher Scientific, Waltham, MA). Pooled indexed libraries were then sequenced on the Illumina NovaSeq 6000 platform with 150-bp paired-end reads.
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bioRxiv - Immunology 2021Quote: ... PD-L1 mRNA expression was quantified using a TaqMan Gene Expression assay (ThermoFisher Scientific, Assay Id: Hs01125301_m1) on a Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μl of the supernatant was then used for the assay with Amplex Red Assay (Life Technologies) according to the producer’s instruction ...
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bioRxiv - Physiology 2021Quote: ... IL-6 (assay ID: HS00985639_m1) and TNF-α: (assay ID: HS00174128_m1) following manufacture’s protocol (Thermo Fisher Scientific). 18s (assay ID ...
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bioRxiv - Genetics 2021Quote: ... and RNA levels were determined via TaqMan qPCR assays for mouse Pycard (ThermoFisher, 4441114, Assay ID: AJMSHN7), with Actb (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... 2.5 μL of cDNA and 2.5 μL of Assay Mix (TaqMan Gene Expression Assays from Applied Biosystems; GAPDH ...
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bioRxiv - Cell Biology 2022Quote: ... pDEST-AD-CYH2 (assay version 1) and pDEST-AD-AR68 (assay version 3) using Gateway Technology (Invitrogen). Attributes of these plasmids are summarized in Suppl ...
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bioRxiv - Neuroscience 2023Quote: ... ε4) and rs7412 (ε2 vs. ε3/ε4) were genotyped using assay C_3084793_20 and assay C_904973_10 (Thermo Fisher), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... The concentrations of actin and other proteins in the assay were determined by Bradford protein assay (ThermoFisher) using bovine serum albumin (BSA ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... targeting human SLC22A10 (Assay ID: Hs01397962_m1) and beta actin (Assay ID: Hs99999903_m1) (Applied Biosystems, Foster City, CA). The qRT-PCR reactions were conducted in a 96-well plate ...
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bioRxiv - Immunology 2023Quote: ... inner salt] cell proliferation and viability assays (CellTiter 96® AQueous One Solution Cell Proliferation Assay, Invitrogen) were performed according to the manufacturer’s instructions with 50 000 cells per well and read at 492nm on a microplate absorbance reader (Multiskan FC microplate photometer ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was assessed either with Bradford’s assay or with EZQ protein quantification assay (Thermo Fisher Scientific) and then kept at −80°C until further processing ...
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bioRxiv - Developmental Biology 2023Quote: ... or snoRNA202 (assay ID: 001232) and snoRNA234 (assay ID: 001234) for mECs (all from Thermo Fisher Scientific) (Wong et al. ...
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bioRxiv - Neuroscience 2021Quote: Cell health in microfluidic chambers was evaluated using NucRed Dead 647 reagent to identify cells with compromised membranes along with membrane-permeant NucBlue™ Live ReadyProbes™ Reagent (both ThermoFisher) to evaluate the total number of cells ...
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bioRxiv - Biophysics 2022Quote: ... cell membranes were labelled with CellMask membrane stain for 10 min prior to collagen digestion following the manufacturer protocol (Thermo Fisher Scientific). To visualise the boundaries of the suspended monolayer ...
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bioRxiv - Biophysics 2021Quote: ... Cells were gently removed from either the top or bottom of the membrane with a cotton swab and the membrane was then mounted on glass coverslips using Prolong Diamond (Thermo Fisher Scientific). The membranes were imaged with a 20x (air ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were separated by dodecyl-sulfate polyacrylamide gel electrophoresis and transferred to Amersham™ Protran® nitrocellulose membranes or PVDF membranes (Invitrogen). The membranes were blocked with 5% non-fat dry milk-TBST (10 mM Tris-HCL [pH 8.0] ...
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bioRxiv - Bioengineering 2021Quote: ... before seeding cells was split in two halves and stained independently using cell membrane fluorescent markers from the Vybrant™ Multicolor Cell-Labeling Kit (V22889, Thermo Fisher Scientific Inc., USA). One half was incubated with 5μM DiO Labeling Solution (Green) ...
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bioRxiv - Microbiology 2024Quote: Membrane and cytosolic proteins were isolated from frozen mouse liver or small intestine (30mg) using the Mem-PER Plus Membrane Protein Extraction Kit (Thermo Fisher Scientific, Catalog no. 89842). Membrane and cytosolic fractions were separated by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were blotted on nitrocellulose membrane (Fisher scientific). The membrane was blocked in 5% milk/TBS-T for 1 h followed by overnight incubation with primary antibody at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was transferred to a nitrocellulose membrane (ThermoFisher, LC2000) and then blocked with Odyssey Blocking Buffer (Li-Cor Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... and transferred to nitrocellulose membranes using iBlot (Life Technologies). For 10K and 100K fractions ...
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bioRxiv - Cell Biology 2020Quote: ... followed by transfer unto Invitrolon™ PVDF membranes (Invitrogen). All blots were run on the XCell SureLock mini-cell system (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... Proteins were transferred to polyvinylidene difluoride membranes (iBlot; Invitrogen). Membranes were blocked with TBST containing 3% BSA and incubated with antibodies against IRF1 (H-8 ...