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Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% heat-inactivated foetal bovine serum (FBS, Gibco), and 100 U/mL of penicillin-streptomycin (P/S ...
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bioRxiv - Molecular Biology 2024Quote: ... using MASCOT through Proteome Discoverer v.1.4 from Thermo Scientific. The abundance value for each protein is calculated as the average of the 3 most abundant peptides belonging to each protein (78) ...
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bioRxiv - Molecular Biology 2024Quote: ... Liver and peripancreatic fat tissues for histology were stored in 4% paraformaldehyde (PFA; Fisher Scientific, Cat # AAJ19943K2) at 4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... used for the duplex qPCR for canola blackleg detection (Fu et al. 2023) were analyzed for potential primer dimers using Multiple Primer Analyzer hosted by Thermo Fisher (https://www.thermofisher.com). One set without any predicted primer dimer was selected to be used in this study and named P-VL (Table 1) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from flash-frozen liver and peripancreatic fat tissues using TRIzolTM (Invitrogen, #15596018; Carlsbad, CA, USA). cDNA was made using the iScript gDNA Clear cDNA Synthesis Kit with DNase treatment (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... and 100 U/mL of penicillin-streptomycin (P/S, Gibco).
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bioRxiv - Molecular Biology 2024Quote: Plaques were digested with an enzymatic cocktail containing Advanced RPMI 1640 (12633-012, Gibco Life Technologies) supplemented with collagenase XI from Clostridium histolyticum at 1.25 mg/ml (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... the Anti-beta actin antibody was reprobed after stripping the membranes using Restore™ stripping buffer (Thermo Fisher Scientific, Cat# 21059).
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bioRxiv - Molecular Biology 2024Quote: ... and Mock plasmid were transiently transfected according to the manufacturer’s protocol into HEK293T cells in full growth medium using Lipofectamine 3000 (Thermo Fisher Scientific, Cat# L3000-015). To assess the transfection efficiency ...
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bioRxiv - Molecular Biology 2024Quote: ... the ligated DNA was transformed into One Shot Stabl3 competent cells (Thermo Fisher Scientific, Cat# C7373-03) and selected on LB agar plates with 100 µg/mL Ampicillin sodium (Fujifilm ...
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bioRxiv - Molecular Biology 2024Quote: ... Midi Protein Gels (Invitrogen™, WG1402BOX) (for BRCA1 detection ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were homogenized in T-PER™ tissue protein extraction reagent (Thermo Fisher Scientific, Cat# 78510) containing Triton(R ...
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bioRxiv - Molecular Biology 2024Quote: ... the proteins were isolated from the supernatant and measured using the Brachidonic-acid assay kit (BCA) (Thermo Fisher Scientific, Cat# 23227). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 0.25% Trypsin-EDTA (Gibco™, Cat# 25200-072). As for counting ...
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bioRxiv - Molecular Biology 2024Quote: ... or Alexa Fluor 488 antibody (Invitrogen, Cat# A11029) for 2 hours at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... and cultured in a humidified 95% air / 5% CO2 incubator at 37°C in a standard Dulbecco’s modified Eagle’s medium (DMEM) (Gibco™, Cat# 10566-016) supplemented with 4.5 g/L D-Glucose ...
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bioRxiv - Molecular Biology 2024Quote: ... using Pierce ECL substrate reagent (Thermo Fisher Scientific, Cat# 32106), the signal was detected on a ChemiDoc machine (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... the synthesized phosphorylated oligos were annealed in a thermocycler (Thermo Fisher Scientific). Next ...
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bioRxiv - Molecular Biology 2024Quote: ... Assessment of RNA purity and concentration was examined using Nanodrop (Thermo Fisher Scientific, Cat# ND-ONE-W), while the RNA integrity number (RIN ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were mounted with ProLong™ Diamond Antifade Mountant (Thermo Fisher Scientific, Cat# P36961). Live cell imaging was conducted with the MitoTracker™ Green FM (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Cells after treatments were detached by trypsin (Life Technologies, 12604013) and resuspended with 1x Laemmli buffer [62.5 mM Tris pH6.8 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then treated with 0.2 × SSC buffer (Ambion, AM9763) with gentle shaking at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and then mounted with ProLong™ Gold Antifade Mountant with DAPI (Invitrogen, P36931). For each experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were incubated with Alexa-488 and Alexa-546-conjugated secondary antibodies (Invitrogen, A32731 and A11126), mounted with ProLong™ Gold Antifade Mountant with DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... and Dulbecco’s modified Eagle medium (Gibco, Cat. #12100046) respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... Epithelial organoids were further digested by 0.05% pre-warmed Trypsin (Life Technologies, 25300), washed in ice-cold Hanks Balanced Salt Solution (StemCell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Collagenase IV (NC9836075, Thermo Fisher Scientific), DNase I ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by magnetic bead clean-up (Dynabeads MyOne Silane, 37002D, Thermo Fisher Scientific). 10X-barcoded full-length cDNA was then amplified by PCR and analysed using Bioanalyzer High Sensitivity DNA kit (5067-4626 ...
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bioRxiv - Microbiology 2024Quote: ... Subsequent cDNA was analyzed in technical triplicate through quantitative PCR using SYBR Green fluorescent probe with ROX (Invitrogen) in a QuantStudio 7 qPCR instrument (Applied Biosystems) ...
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bioRxiv - Microbiology 2024Quote: Primary CF-HBECs from 4 donors were polarized on 24 mm Transwell filters and washed with DPBS (ThermoFisher Scientific) before treatment ...
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bioRxiv - Microbiology 2024Quote: ... The cells were cultivated in 24-well plates using Dulbecco’s Modified Eagle Medium (DMEM, Gibco, USA) supplemented with 10% Fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... incubated with secondary antibodies (donkey anti-mouse 488 Invitrogen, A-21202, donkey anti-rabbit 568, Invitrogen, A-10042, donkey anti-goat 647, Invitrogen, A-21447) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 1% antibiotic mixture (penicillin-10,000 U/ml and streptomycin-10 mg/ml, ThermoFisher, USA), and 1% non-essential amino acids mixture (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... in a QuantStudio 7 qPCR instrument (Applied Biosystems). Cp values for each target gene (primers HCCS_qF1 ...
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bioRxiv - Microbiology 2024Quote: ... 50 µg/ml of hygromycin B (ThermoFisher, USA), and kept in a 5% CO2 atmosphere at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... and total RNA was extracted using the RNeasy Plus Mini kit (Ambion, Lithuania). The RNA was quantified using a Biophotometer (Eppendorf ...
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bioRxiv - Microbiology 2024Quote: ... developed using the SuperSignal West Pico PLUS enhanced chemiluminescence reagent (Thermo Fisher 34577), and imaged on an Invitrogen/Thermo Fisher iBright 1500 imager.
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bioRxiv - Microbiology 2024Quote: ... 20 µg of mitochondrial protein was mixed with 1X Native-PAGE sample buffer (Invitrogen BN2003) supplemented with 1% digitonin and incubated on ice for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... and the reverse transcription was performed using Superscript FirstStrand Synthesis for RT-PCR (Invitrogen, CA, USA). cDNA was amplified using the Master Mix kit (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... adapters were ligated to the amplicons in a PCR reaction using the P5 and P7-bearing adapter primers and the Phusion High-Fidelity DNA Polymerase kit (Thermo Scientific). Amplicons were sized and purified using an AmpurXP magnetic bead capture (Agencourt) ...
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bioRxiv - Microbiology 2024Quote: ... (Gibco BRL, ThermoFisher Scientific Inc., Waltham, MA, USA) followed by the addition of two-fold serially diluted RDE-treated ferret sera at 50 µL per well starting with 1:20 dilution in virus growth medium containing 1 µg/mL TPCK-treated trypsin ...
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bioRxiv - Microbiology 2024Quote: ... Plates were washed five times with PBS and then twice with distilled water (dH2O) before addition of 5-bromo-4-chloro-3-indolyl-phosphate/NBT (BCIP/NBT) one-step solution (Thermo Fisher Scientific) and incubation at 37°C for approximately 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... The samples were stored in RNA Later solution (Invitrogen) at -70 °C until use ...
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bioRxiv - Microbiology 2024Quote: Vero E6 cells (ATCC C1008) were maintained in Eagle’s minimum essential medium (Gibco; Thermo Fisher Scientific, MA, USA) supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... and 20 U/L of turbo DNase (Ambion). Then ...
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bioRxiv - Microbiology 2024Quote: Vero E6 cells (ATCC C1008) were maintained in Eagle’s minimum essential medium (Gibco; Thermo Fisher Scientific, MA, USA) supplemented with 5% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... (Gibco BRL ...
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bioRxiv - Microbiology 2024Quote: ... The non-capsid-protected DNA was eliminated with 2.5 units per mL of DNase I following the manufacturer’s procedure (Cat 18047-019, Invitrogen, MA, USA). The treated VLPs were then concentrated with AMICON and recuperated in 200 μL SM buffer ...
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bioRxiv - Microbiology 2024Quote: Hela cells were cultured in DMEM supplemented with 10% foetal bovine serum (Invitrogen). Plasmids pEGFPC1 and pEGFPN1 ...
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bioRxiv - Microbiology 2024Quote: ... The quality of RNA was assessed using a NanoDrop (ND-1000; Thermo Scientific, USA). cDNA was synthesized using 1 µg of RNA ...