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Citations for Thermo Fisher :
8851 - 8900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... F-actin was stained with Phalloidin (Molecular Probes, A12380 or A22287). Nuclei were stained with DAPI (Molecular Probes ...
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bioRxiv - Cell Biology 2024Quote: ... 1× nonessential amino acids (Gibco, 11140-035), 100 U·mL−1 penicillin/100 μg·mL−1 streptomycin ...
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bioRxiv - Cell Biology 2024Quote: ... transfected cells were cultured with 10% FBS EV-depleted [46] in DMEM (Gibco) and maintained at 37°C in a humidified incubator equilibrated with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Gut overview images were acquired on a Leica M165FC microscope with Leica DFC3000G camera or EVOS M7000 (ThermoFisher Scientific). All images were processed and analysed with FiJi (Schindelin et al. ...
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bioRxiv - Cell Biology 2024Quote: ... the remaining cells were then plated in in 96-well plates in B cell Medium (RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA, 21875034) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: ... and 647-conjugated secondary goat antibodies (Molecular Probes) were used ...
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bioRxiv - Cell Biology 2024Quote: ... on a 40X air objective using the EVOS M7000 (ThermoFisher Scientific). Image files were processed in FiJi ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained with DAPI (Molecular Probes, D1306) or Hoechst (Molecular Probes ...
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bioRxiv - Cell Biology 2024Quote: ... Before acquisition cells were labelled with Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific, 65-0865-14) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 µg/mL Gentamycin (Gibco 15750-037). Peritoneal lavage was performed post-mortem by injecting 10 mL staining buffer in the peritoneal cavity using a 27g needle ...
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bioRxiv - Cell Biology 2024Quote: ... or Hoechst (Molecular Probes, H1399).
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bioRxiv - Cell Biology 2024Quote: ... The qRT-PCR was run on a StepOnePlus Real-time PCR system (Applied Biosystems, Foster City, CA, USA). Gene expression of individual mRNAs was normalized to HPRT using the ΔC(t ...
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bioRxiv - Cell Biology 2024Quote: ... mixed in a 1:1 ratio with ImjectTM Alum Adjuvant (Thermo Fisher 77161) in a volume of 200µL per mouse ...
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bioRxiv - Cell Biology 2024Quote: ... 1 x NEAA (Gibco, 11140-035), 100 units/mL penicillin ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mm sodium pyruvate (Gibco, 13360-039), 1× nonessential amino acids (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... goat α-mouse IgG AF568 (Thermo Fisher, A11031, 1:1000), goat α-rabbit IgG AF488 (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... 1 mmol/L sodium pyruvate (Gibco, 11360-039), 1 x NEAA (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... and F-12 (Gibco) at 1:1 ratio ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 5 μg/mL Hoechst 33342 (Invitrogen) in cell culture medium for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... or MCUB were expanded into two expression plates (245×245 mm, NUNC, 9407400) to obtain roughly 250 million cells ...
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bioRxiv - Cell Biology 2024Quote: ... We performed cDNA synthesis using the High Capacity RNA-to-cDNA kit (Applied Biosystems), according to manufacturer instructions.
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bioRxiv - Cell Biology 2024Quote: ... Constructs were then cloned either into pcDNA-DEST40 Vector (Thermo Fisher Scientific, 12274015) or pLX304 for expression in mammalian cells in frame with a C-terminal V5-His6 and V5 tag ...
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bioRxiv - Cell Biology 2024Quote: ... The qPCR run and data analysis were performed using QuantStudio (Applied Biosystems). The following primers were used for quantifying the mRNA levels of GAPDH ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmids for Cas9 and homology repair were transfected to cells with Lipofectamine 2000 (Invitrogen). Successful C-terminal knock-in cells were identified by fluorescence activated cell sorting (FACS ...
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bioRxiv - Cell Biology 2024Quote: ... or CellTrace Far Red (Thermo Scientific) staining was performed according to manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Translation sites in fixed cells were labeled anti-Flag labeling (FUJIFILM, Wako) followed by Cy3 or Alexa 594 conjugated anti-mouse secondary antibodies (Jackson Immunoresearch and Invitrogen). In live cells ...
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bioRxiv - Cell Biology 2024Quote: ... penicillin and streptomycin (Thermo Scientific) at 100 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... We cultured the cells in DMEM (Gibco) and F-12 (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... L-glutamine (Thermo Scientific) at 0.5 μg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-rat Alexa Fluor 647 (Molecular Probes). Nanobody (Atto 542 FluoTag-X2 anti-Mouse Kappa light chain ...
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bioRxiv - Cell Biology 2024Quote: ... si-PRELID1: 5’-CCCGAAUCCCUAUAGCAAA-3’) were transfected using Lipofectamine RNAiMAX (Thermo Fisher Scientific, 13778075). Assays were normally carried out 48 h after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... The 2D well of the pre-cast NuPAGE 4%-12% Bis-tris 2D well gel (Thermo Fisher Scientific, NP0326BOX) was washed and filled with 1x MOPS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... one well was loaded with 5 µg of input mitochondria previously diluted in 5 µL of 2x LDS dye (Thermo Fisher Scientific, 84788) with 2.75 mM β-mercaptoethanol (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... The TN-C solution was prepared by diluting TN-C to 300 ng/mL in PBS (Gibco, city, state, USA) for the experimental group (n=3) ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.4) in the presence of 0.003% Pluronic F-127 (Thermo Fisher Scientific, P6867) and 150 μM sulfinpyrazone (Sigma Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... qRT-PCR was performed using a 7500 Fast Real-Time PCR System (Applied Biosystems). GAPDH mRNA expression levels were used as a baseline and target gene expression levels were corrected for relative quantitative analysis.
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bioRxiv - Cell Biology 2024Quote: ... The CyQUANT® Cell Proliferation Assay Kit (Thermo Fisher Scientific, C7026) was used to normalize differences in cell density between wells ...
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bioRxiv - Cell Biology 2024Quote: ... with 2.75 mM β-mercaptoethanol (Thermo Fisher Scientific, 21985-023) and RIPA buffer (total volume 10 µL ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... An equal amount of RNA from each sample was used to generate complementary DNA (cDNA) with the SuperScript™ III First-Strand Synthesis SuperMix kit (Thermo Fisher Scientific, 18080400). The resulting cDNA was diluted 1:8 in nuclease-free water and analyzed by real-time PCR using the following TaqMan assays ...
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bioRxiv - Cancer Biology 2024Quote: ... DynaBeads Protein G magnetic beads (Life Technologies 10004D) were prepared 24 h in advance by washing 2x in NT-2 buffer (5x NT-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% bovine calf serum (Gibco, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 10% bovine calf serum (Gibco, ThermoFisher Scientific, Grand Island, NY, USA), 1% penicillin/streptomycin (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were trypsinized (0.05% Trypsin-EDTA, Gibco, ice-cold), scraped with a cell scraper ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were cultured in Dulbecco’s Modified Eagle Medium (Gibco, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were then cultured with the indicated compound for a total of 6-days before cell lysis and quantification of proliferation using the Quant-iT™ PicoGreen dsDNA Assay Kit (ThermoFisher Scientific, Grand Island, NY, USA). For long-term proliferation (12-days) ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPA Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 µg/mL streptomycin (Gibco). Flasks and 96 well plates that were used with the non-cancerous line HFL1 were pre-treated with 0.01 mg / ml Collagen Type 1 (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was then synthesized using the SuperScript™ III Reverse Transcriptase kit (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Flasks and 96 well plates that were used with the non-cancerous line HFL1 were pre-treated with 0.01 mg / ml Collagen Type 1 (Gibco) and rinsed with PBS (Fisher).