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Citations for Thermo Fisher :
651 - 700 of 1318 citations for Vertical Electrophoresis Comb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The proteins were separated by electrophoresis under denaturing conditions on NuPage Bis-Tris Pre-Cast gels (Invitrogen, Carlsbad, CA) for 60 min at 200 Volts and transferred onto nitrocellulose membranes (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... Fragment analysis was done by capillary electrophoresis using LIZ-500 as internal size standard (Gene Scan 500, Applied Biosystems) and the typing data was analyzed by using BioNumerics software version 7.1 (Applied Maths ...
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bioRxiv - Microbiology 2021Quote: ... and RNA integrity was assessed with gel-electrophoresis and quantified using a Nanodrop (Thermo Fisher Science, Waltham, MA, USA). Library were prepared and singleend 50 bp sequenced on the DNBseq platform at BGI (Hong Kong ...
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bioRxiv - Microbiology 2021Quote: ... After analysis through electrophoresis on a 1% agarose gel as well as a Qubit 2.0 (Thermo Scientific, Waltham, USA), a NEBNext Ultra™ ‖ DNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Protein quality and quantities were checked by SDS-PAGE electrophoresis (12% polyacrylamide gels) and UV spectrometry (Nanodrop, Thermo Scientific). A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed using precast 4-12% polyacrylamide gradient gels (ThermoFisher, NW04122BOX), submerged at 4°C in Bolt™ MOPS SDS Running Buffer (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Gel electrophoresis was performed at 130 V for 70 min in Bolt MOPS SDS Running Buffer (ThermoFisher Cat# B0001). Proteins were subsequently transferred to a 0.45 μm polyvinylidene difluoride membrane ...
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bioRxiv - Plant Biology 2021Quote: ... PCR fragments were isolated from 1 % agarose gel after electrophoresis and purified by GeneJET Gel Extraction Kit (Thermo Fisher). The fragment generated was inserted into the PJET1.2/blunt cloning vector (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The harvested DNA was detected by the agarose gel electrophoresis and quantified by Qubit 3.0 (Thermo Fisher Scientific, USA). Whole-genome sequence determinations of strain CS1 were carried out with the PacBio (Pacific Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Equal quantities of cellular DNA from different conditions of the experiments were subjected to gel electrophoresis in 1.4% agarose gel with SYBR safe dye (Invitrogen). Agarose gel electrophoresis was done at 50V (5V/cm ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of proteins obtained after TCA precipitation were separated by 4-12% polyacrylamide gradient gel electrophoresis (NuPAGE-Invitrogen) in 1X MES buffer (50 mM MES ...
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bioRxiv - Immunology 2022Quote: ... The quality and quantity of RNA were evaluated by agarose gel electrophoresis and Qubit RNA HS Assay Kit (Invitrogen).
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bioRxiv - Microbiology 2022Quote: ... RNA quality and quantity were assessed using gel electrophoresis and using a NanoDropTM 2000/2000c Spectrophotometer (Thermo Fisher Scientific). All RNA extractions were carried out in triplicate.
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bioRxiv - Genetics 2019Quote: ... The quality and concentration of the extracted DNA was verified using gel electrophoresis and Qubit 2.0 fluorometer (Life Technologies). Extracted genomic DNA was used for preparing Illumina RAD sequencing libraries based on previously described protocols (Baird et al ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of protein (30-50 µg) were fractionated on 4-12% gradient SDS-polyacrylamide gel electrophoresis gel (Invitrogen) and transferred to PVDF membrane (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... The integrity of DNA was checked on gel electrophoresis and quantified using a NanoDrop ND1000 Spectrophotometer (Thermo Scientific, USA).
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bioRxiv - Neuroscience 2020Quote: ... to be subjected to electrophoresis at 21 V for 30 min and stained in 2.5 μg/mL propidium iodide (Cat# P3566, Invitrogen) for 20 min ...
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bioRxiv - Immunology 2021Quote: ... Samples were resolved by electrophoresis on 4-12% Bis-Tris gels with MOPS Running Buffer (Life Technologies NuPAGE system) and transferred to nitrocellulose membrane by electrophoresis at 100V for 1 hour in cold transfer buffer (Life Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... RNA quality was tested using agarose gel electrophoresis and concentration was quantified using a Nanodrop Lite spectrophotometer (Thermo Fisher). Complementary DNA was created by reverse transcription using an iScript cDNA kit (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... loaded on leaf area basis and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation ...
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bioRxiv - Genetics 2020Quote: ... Whole cell protein samples were resolved by SDS-PAGE electrophoresis and analyzed by Western blot using anti-V5 (1:40,000) (Invitrogen) with goat anti-mouse HRP (1:40,000) ...
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bioRxiv - Plant Biology 2021Quote: ... and separated by polyacrylamide gel electrophoresis (Nu-PAGE 4-12% Bis-Tris gel, Invitrogen, Life Technologies Corporation, Carlsbad, CA). Proteins were transferred to a nitrocellulose membrane and probed against photosynthetic proteins with antibodies according to manufacturer’s protocols (Agrisera ...
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bioRxiv - Genomics 2021Quote: The integrity and concentration of RNA and DNA was analyzed by agarose gel electrophoresis and Qubit 2.0 fluorometer (ThermoFisher), respectively ...
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bioRxiv - Biochemistry 2021Quote: ... Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) was performed on a NuPAGE Novex Bis-Tris gel following manufacturer’s instructions (Invitrogen). Protein/ACE2 or protein/mAb attached on magnetic beads or protein control in solution were mixed with 4X Sample Buffer and deionized water (1:3 v/v ...
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bioRxiv - Genomics 2021Quote: ... The quality and quantity of the DNA were measured via standard agarose gel electrophoresis and with a Qubit□4.0 Fluorometer (Invitrogen). Sequencing library was constructed and sequenced by Nanopore PromethION platform (Oxford Nanopore Technologies) ...
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bioRxiv - Genomics 2021Quote: ... The quality and quantity of the DNA were measured via standard agarose gel electrophoresis and with a Qubit□2.0 Fluorometer (Invitrogen). Sequencing library was constructed and sequenced by Illumina Novaseq platform (Illumina).
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bioRxiv - Genetics 2021Quote: ... were taken to reducing sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE; Gels: ThermoFisher NuPAGE 4 to 12 %, Bis-Tris). The separated proteins were transferred to nitrocellulose membranes (0.2 µm ...
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bioRxiv - Bioengineering 2022Quote: ... Proteins were separated by electrophoresis in NuPAGE 4-12% Bis-Tris gels run with 1x MOPS buffer (Invitrogen NP0323) and transferred to an iBlot nitrocellulose membrane (Invitrogen IB301002) ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Fluorescently labelled PCR products were resolved by electrophoresis on an ABI 3730xl capillary sequencer (Applied Biosystems, Waltham, MA, USA) and allele sizes were scored using GeneMarker v ...
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bioRxiv - Cell Biology 2021Quote: ... RNA integrity and concentration were confirmed by agarose gel electrophoresis and by using the Nanodrop 2000 (Thermo Fisher Scientific) respectively ...
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bioRxiv - Genetics 2021Quote: The products were then separated by capillary electrophoresis using an ABI Prism® 3730 Genetic analyzer (Applied Biosystems, USA) (Koumi et al. ...
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bioRxiv - Genomics 2020Quote: ... DNA quality was confirmed by agarose gel electrophoresis and quantification was performed on a NanoDrop 8000 spectrophotometer (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Products were separated by agarose gel electrophoresis and purified with the PureLink Quick Gel Extraction Kit (Thermo Fisher K210012). Samples were then diluted to 2–4 nM ...
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bioRxiv - Genomics 2019Quote: ... and the resulting PCR product was subjected to gel electrophoresis (E-Gel™ EX Agarose Gels, 2% [ThermoFisher G402002]), and gel extraction (Wizard™ SV Gel and PCR Cleanup System [Promega A9282] ...
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bioRxiv - Neuroscience 2020Quote: ... Reducing and non-reducing protein samples were separated by gel electrophoresis and transferred to 0.2μm nitrocellulose membranes using the Bolt SDS-PAGE system (Life Technologies).
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bioRxiv - Molecular Biology 2022Quote: ... The digested products were analysed by electrophoresis on a 1.5% agarose gel stained with SYBR Safe DNA Gel Stain (InvitroGen). The experiment was run for 1 h at 145 V and the gel was visualized under UV light using a BioSpectrum Imaging System (Ultra-Violet Products Ltd.) ...
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bioRxiv - Neuroscience 2022Quote: ... RNA integrity and yield were assessed by 1.5% agarose gel electrophoresis and UV-visible spectroscopy (NanoDrop, Thermo Fisher Scientific), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... Sample buffer was added and the proteins resolved by electrophoresis on a NuPAGE 10% BisTris mini gel (Thermo Fisher). The gel was stained using SimplyBlue Safestain (Thermo Fisher ...
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bioRxiv - Genomics 2022Quote: ... The amplified products were checked on 2% agarose electrophoresis and purified using Purelink PCR purification kit (Invitrogen, United States). The purified amplicons were quantified on Qubit 2.0 using qubit dsDNA BR assay kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... at 100 V and room temperature for 45 min in XCell SureLock Mini-Cell Electrophoresis System (Thermo Fisher Scientific). Gels were visualized using Odyssey CLx (Li-COR Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... Product sizes were evaluated using gel electrophoresis (1.5% agarose) with SYBR Safe DNA Gel Stain (Fisher Scientific, Chicago, IL) and purified using a Qiagen PCR Purification Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The 70kd Ssa1,2 proteins were isolated by electrophoresis on an SDS polyacrylamide gel followed by staining with colloidal coomassie blue (Invitrogen). The 70kd band was excised from the gel and subjected to reduction ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR fragments were analyzed by gel electrophoresis with 1.8% agarose gels in TBE buffer containing 20000x SYBR Safe (S33102, Invitrogen). 5 μl of PCR products were loaded in each well ...
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bioRxiv - Genomics 2022Quote: RNA concentration and quality were determined by gel electrophoresis in 1% UltraPure™ agarose (Invitrogen Corp., Carlsbad, California, USA) in 1X TBE running buffer at 100 V for 30 min ...
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bioRxiv - Genomics 2022Quote: ... Quality and quantity of the DNA were measured via standard agarose-gel electrophoresis and with a Qubit 3.0 Fluorometer (Invitrogen). Nanopore sequencing libraries of S ...
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bioRxiv - Molecular Biology 2022Quote: ... Gels were run at 100V until completion of electrophoresis and visualised using colloidal coomassie blue (Thermofisher Scientific, Paisley, UK) according to manufacturer’s guidelines.
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bioRxiv - Plant Biology 2024Quote: ... The quality and quantity of the RNA samples were analysed by agarose gel electrophoresis and Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... after agarose gel electrophoresis and blunt end cloning was performed into pCR™-Blunt II-TOPO™ Vector (Invitrogen). Site-directed mutagenesis was performed on plasmid that carried E gene to introduce the desired mutation using primer pairs (Table S2) ...
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bioRxiv - Plant Biology 2023Quote: ... DNA degradation and quantity were assessed by resolving sample and standard DNA by agarose gel electrophoresis (Thermo Fisher Scientific). The isolated DNA was subjected to next-generation short read sequencing (NGS ...
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bioRxiv - Immunology 2024Quote: ... and uterine tissue lysates (75 μg per well) were subjected to electrophoresis in sulfate-polyacrylamide gels (Cat# NP0336BOX; Invitrogen). The proteins were transferred onto nitrocellulose membranes (Cat# 1620145 ...