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Citations for Thermo Fisher :
8751 - 8800 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... 2 µL per sample were desalted using a ProSwift™ RP-4H 1 ×50mm column (Thermo Fisher Scientific) followed by injection into a maXis II ETD ESI LCMS (Bruker Daltonics) ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... Undeveloped photoresist was washed off with SU-8 developer (1-methoxy-2-propanol acetate, Fisher Scientific, Waltham, MA).
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bioRxiv - Cancer Biology 2024Quote: ... and 1:400 Alexa Fluor 488 F (ab′)2 goat anti−mouse IgG (Thermo Fisher Scientific, A-11017). These slides were washed with 1X PBS and mounted with Vectashield plus antifade mounting medium (Biolynx ...
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bioRxiv - Cell Biology 2024Quote: ... 2% SDS lysis buffer using tip-probe sonication (10 X 0.5s pulses; Level 1) (Fisher Scientific, Waltham, MA). Protein was quantified by 660 nm spectrometry using detergent-compatible reagents ...
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bioRxiv - Cancer Biology 2024Quote: ... cell nuclei were stained with 1 µg 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, Thermo Fisher Scientific, D1306) for 15 min in a humidified incubator at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... and 4 dpf were injected with 1–2 nL of dextran tetramethyl rhodamine (molecular weight 2000 kDa, Invitrogen) at a concentration of 10 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with 1 μg of plasmid DNA mixed with 2 μL of lipofectamine 2000 (Invitrogen #11668019) on 37 °C for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... cell nuclei were stained with 1 µg 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, Thermo Fisher Scientific, D1306) for 15 min in a humidified incubator at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated for 2 h with Alexa Fluor 488 donkey anti-goat IgG (Invitrogen, 1:400, #A11055), Alexa Fluor 568 donkey anti-mouse IgG (Invitrogen ...
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bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml or Dynabeads Mouse T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Cell Biology 2020Quote: ... guide RNA plasmid and a plasmid expressing SpCas9 at a ratio of 2:1:1 (total 12ug) using Neon electroporator (Life Technologies) and a 30ms ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were transiently transfected with HA-Flag-GHRHR-Rluc8 or HA-Flag-SV1-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using Lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Microbiology 2021Quote: ... fixed and permeabilized specimens were stained for 1 hour at room temperature by adding PermQuench buffer containing 1 μg/mL Hoechst 33342 and 2 μg mL-1 CellMask™ Deep Red plasma membrane stain (H32721, Invitrogen). After staining ...
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bioRxiv - Neuroscience 2020Quote: ... with 5% DMSO for at least 2 nights at room temperature with the following reagents: (1) 1:20 AlexaFluor 594 phalloidin (ThermoFisher #A12381) (Figure 3F ...
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bioRxiv - Neuroscience 2021Quote: ... Striatum slices were further stained for 1-2 h at RT with NeuroTrace 435/455 (1:100 diluted in PBS, Invitrogen, #N21479), a fluorescent Nissl staining ...
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bioRxiv - Cell Biology 2021Quote: ... Cardiac cells were subsequently thawed in RPMI+B27 media (RPMI 1640 supplemented with 2% B-27 Supplement minus vitamin A, 1% GlutaMAX and 1% Penicillin/Streptomycin- All from ThermoFisher Scientific) with Rock inhibitor (Selleck Chemicals ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transiently transfected with HA-GLP-1R-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Immunology 2020Quote: ... monocytes were plated in low-serum media (1% FBS) for 1-2 hours in 12-well Nunc-coated plates (ThermoFisher Scientific) to promote adherence ...
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bioRxiv - Biochemistry 2020Quote: The cDNA coding for SARS CoV-2 Nsp1 1-180 Nsp2 1-19 8His was synthesised by GeneArt (Thermo Fisher Scientific) adding NcoI and NotI sites at the 5’ and 3’ ends ...
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bioRxiv - Plant Biology 2022Quote: ... To each sample 0.7 µL of reverse transcription mix (8:2:1:1 of Superscript IV 5X Buffer, 100 mM DTT, RNase Inhibitor, Superscript IV; ThermoFisher Scientific) was added ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Developmental Biology 2022Quote: ... tissues were incubated with 1:200 anti-rat IgG 647 for 2 h at room temperature with 1:500 phalloidin TRITC (Fisher Scientific). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were washed in block six times for 1 hour and incubated in secondary antibodies conjugated to fluorescent dyes diluted 1:250 in block with added 4’,6-diamidino-2-phenylindole (DAPI; 1:500 dilution of 1 mg/ml stock, Thermo Fisher) for 2 nights at 4°C.
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bioRxiv - Neuroscience 2024Quote: ... To visualize Gi-DREADD-mCherry expression in rCA2 of injected mice sections were stained with 1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... In order to visualize AAV-retro positive cells sections were stained for mCherry (1⁰ rat anti-mCherry (Life Technology #M11217) 1:500 + 2⁰ anti-rat 568 (Invitrogen #A11077) at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... A subset of sections was stained for calbindin+ cells (1⁰ rabbit anti-calbindin (Swant #CB38) 1:500 + 2⁰ anti-rabbit-633 (Invitrogen #A21071) 1:500) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... slides were incubated in the dark at room temperature for 2 hours with 1:1000 DAPI and 1:500 AlexaFluor secondary antibodies (Thermo Fisher) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2-hour incubation with secondary antibody (1:500 in 1% BSA in PBST; AF488 anti-rat, Invitrogen, A-11006) at room temperature ...
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bioRxiv - Biochemistry 2023Quote: The datasets of ULK1C (2:1:1) core and the ULK1C:PI3KC3-C1 mixture were recorded at a 300 kV Titan Krios microscope (Thermo Fisher Scientific) equipped with X-FEG and energy filter set to a width of 20 eV ...
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bioRxiv - Biochemistry 2023Quote: ... fresh P3 virus from γ-TuRC-GFP and γ-TuSC were mixed in a 1:1 ratio and used to infect 1.8-2 L of High five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 30 mL of the γ-TuRC-GFP and γ-TuSC virus mixture as above ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µL of a 1:100 dilution of the ERCC RNA Spike-In Mix 1 (Ambion by Life Technologies, cat# 4456740) was added to the total RNA ...
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bioRxiv - Neuroscience 2023Quote: ... were selected and processed as described above with primary antibody Rabbit anti-Cox-2 (1:1k, Cayman Chem, 160123) and secondary antibody AF555 donkey anti-rabbit (1:200, ThermoFisher, A31572).
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 ml of 56°C warm gel solution (1 volume RPMI/20% FBS, 1 volume 2x HBSS, 2 volumes 2.5% agarose in water (ultrapure agarose, Invitrogen, #16500-100)) were cast per 35 mm dish and allowed to solidify ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was then incubated for 1 hr at RT with 2° anti-rabbit HRP antibody (1:10,000, ThermoFisher, Cat #31458). The membrane was washed 5 times 5 min in TBS-T prior to visualization with SuperSignal West Pico Chemiluminescent Substrate (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: A 1 × 1 cm of mouse skin encompassing the wound site was excised into 2 mL of RNAlater stabilization solution (Invitrogen, USA) and incubated overnight at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Neuroscience 2024Quote: Rhod2-AM (Fig. 2 and Fig. S2) or Oregon Green 488 BAPTA-1-AM (OGB-1; other figures; all ThermoFisher Scientific) were bolus-loaded as described 55 with minor modifications ...
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bioRxiv - Cell Biology 2024Quote: ... were added at a dilution of 1:500 in blocking solution (Aligent Antibody Diluent #S202230-2) containing 1:100 Phalloidin 594 (ThermoFisher #A12381) to cells and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed with PBS and starved with smooth muscle cell growth medium 2 containing 1% FBS + 1% insulin-transferrin-selenium (Gibco, 41400045) + 1% P/S overnight ...
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bioRxiv - Immunology 2024Quote: ... or AG1478 (500 nM) for 72 hours in smooth muscle cell growth medium 2 containing 1% FBS + 1% insulin-transferrin-selenium (Gibco, 41400045) + 1% P/S ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were incubated with fluorophore- conjugated secondary antibody in 2% milk in PBS with 0.3% triton X-100 for 1 hour (1:200, Alexa 555, Invitrogen A-21424) then mounted with DAPI-containing Fluoromount medium (Southern Biotech 0100-20) ...
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bioRxiv - Neuroscience 2024Quote: ... in 0.1 M PB and stained with Alexa Fluor (AF) 647-conjugated streptavidin (1:300 diluted from 2 mg ml−1 stock, Invitrogen S32357) overnight in blocking/permeabilising solution ...