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Citations for Thermo Fisher :
8701 - 8750 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... were blocked for 2 hr at 4 °C in lysis buffer containing 1 mg/ml yeast tRNA (Ambion) and washed twice with 1 ml lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... From D4 on N2/B27 (Neurobasal medium/GLUTAMAX/2% B27 w/o vitamin A [Gibco]/1% N2 [Gibco] was added to the KSR medium every second day in increments of 25% ...
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bioRxiv - Neuroscience 2022Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch ...
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bioRxiv - Immunology 2022Quote: ... incubating for 2 hrs at ambient temperature with Alexa Fluor donkey anti-Rabbit 555 1:200 (A31572, Invitrogen), DAPI for 5 minutes and then cover slipping the tissue sections with Aqua-Poly mount (Polysciences) ...
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bioRxiv - Microbiology 2023Quote: ... parasite nuclei were visualized by incubating samples with 1-2 μg/mL Hoechst 33342 (Thermo Scientific Pierce 62249) for 10-20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained by incubation with 0.5 – 1 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, D1306, Invitrogen) in PBS for 10 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 1 h in anti-HA 2-2.2.14 IgG1 mouse mAb (ThermoFisher Catalog# 26183; RRID:AB_10978021) diluted 1:2000 and following 3 x 10 min washes in 0.1 M PB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... sheared into pieces 1–2 kb in size and cloned using the TOPO Shotgun Subcloning Kit from Invitrogen. Colonies containing inserts were collected ...
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bioRxiv - Physiology 2023Quote: ... containing 1% Triton X-100 and 2% Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Scientific, Waltham, MA, USA). Lysates were sonicated and centrifuged ...
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bioRxiv - Physiology 2023Quote: ... sections were incubated 2 h at RT with A568 donkey anti-rabbit secondary antibody (1:500; Invitrogen; A10042) in [PB 0.2% Tx + 1% donkey serum] ...
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bioRxiv - Developmental Biology 2023Quote: ... Bones were flushed using a 26-gauge needle of a 1-ml syringe with HBSS+2% FCS (Gibco). BM clumps were re-suspended by gentling aspirating with a 19-gauge needle ...
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bioRxiv - Microbiology 2023Quote: ... with 100 µL cell suspension at the density of 2×105 cells mL-1 in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 expression vectors (pcDNA3.1_ spike_del19) using Expifectamine Enhancer according to the manufacturer’s protocol (Thermo Fisher). Two days later ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Odour dilutions (10-2 for butanol and 10-1 linalool) in mineral oil (Acros Organics, product code: 124020010) were chosen due to their overall similar strength of response in the cockroach antennal lobe (Paoli et al ...
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bioRxiv - Cancer Biology 2023Quote: ... and sub cultured by incubation for 2 minutes in 1 ml of 0.25% Trypsin-0.53mM EDTA (Gibco, 25200072) at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was synthesized from 1~2 μg of RNA using Superscript IV VILO master mix (Thermo Fisher #11756050). The cDNA was diluted 3-fold and 1 μl of diluted cDNA was used as template ...
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bioRxiv - Microbiology 2023Quote: ... Complexes were precipitated by centrifugation and washed three times in PBS supplemented 0.02% Tween 20 and resuspended in 100 uL PBS prior to elution with 40 uL of 2 parts 0.1M glycine pH 2.8 and 1 part NuPage sample buffer and reducing agent (Invitrogen). Samples were heated at 70°C for 10 min prior to magnetic removal of Dynabeads and storage at -20°C.
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bioRxiv - Genetics 2023Quote: ... The membrane was boiled for 30 min in water and incubated for 1 hour in 5% skim milk in TBS pH 7.4 then for 2 hours in primary antibody (1:2,000 ubiquitin monoclonal antibody 13-1600 Invitrogen) and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP ...
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bioRxiv - Immunology 2023Quote: ... This step was followed by 2 hours incubation in Alexa Fluor anti-rabbit 488 (1:350, Life Technologies) and horse anti-mouse biotinylated (1:350 Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Microbiology 2023Quote: ... 0.05mM 2-mercaptoethanol and 1% Pen/Strep) and enumerated using the automated cell counter Countess II (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Fura-2 fluorescence was calibrated with defined Ca2+ buffers generated with a Calcium Calibration Buffer Kit #1 (Invitrogen).
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bioRxiv - Neuroscience 2023Quote: Batches 1 and 2 were labeled using the TMT 11-plex kit (ThermoFisher 90406 – Lot# TG273545 and TG273555) while batch 3 was labeled with TMTPro (ThermoFisher A44520 – Lot# VH311511 ...
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bioRxiv - Physiology 2023Quote: ... slides were stained with 1:10,000 DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific; Cat. No.: D3571) for 10 min at room temperature before coverslips were applied with PBS and glycerol (1:1 ...
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bioRxiv - Cell Biology 2023Quote: ... 1:2 diluted whole cell extract in buffer C was added to Dynabeads Protein G (Thermo Fisher Scientific) pre-incubated with HEXIM1 antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNAs for ALKBH5 (1; s29686 and #2; s29688) and non-targeting control (4390844) were obtained from Thermo Fisher Scientific and transfected using DharmaFECT 4 Transfection Reagent (Horizon Discovery ...
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bioRxiv - Physiology 2023Quote: ... cells were resuspended in FACS buffer (1% of BSA in PBS) and 2 μg/ml DAPI (D1306; Invitrogen) was added immediately before sorting using FACS Aria Cell Sorter (BD Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was made from 1-2 µg of total RNA using the first strand cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... hippocampi were dissected from 1-to 2-d-old mouse neonates in cold Hank’s buffer solution (HBSS) (Gibco) supplemented with 0.08% D-glucose (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... hippocampi were dissected from 1 to 2-d-old mouse neonates in cold Hank’s buffer solution (HBSS) (Gibco), supplemented with 0.08% D-glucose (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Cell Biology 2024Quote: ... and then incubated for 1 hour in secondary antibodies with 0.5 μg/ml 4,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Antibodies used are listed in Table S6 ...
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of sample were mixed with 2 µL of 1 U/µL DNase I (ThermoFisher Scientific, USA) and 13 µL of 10X DNase I reaction buffer with MgCl2 (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... 100 nM) were loaded on 2 % agarose gel containing 1 × fluorescent dye SYBR Safe DNA Gel Stain (Invitrogen), run at 150 V for 45 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were rinsed and incubated with 4,6-diamino-2-phenyindole dilactate (DAPI, 1:1000, Molecular Probes, R415) for 15 minutes ...
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bioRxiv - Genetics 2024Quote: ... tube 2 (well specific tube, 2A, 2B, etc.) contained 1 µl of P3000 enhancer (Thermo Fisher, REF-L3000008), 1 µg/µl of plasmid DNA ...
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bioRxiv - Physiology 2024Quote: ... cells were rinsed with Tyrode’s buffer and incubated for 13 – 15 minutes in Tyrode’s buffer with 1 μM Fura-2 AM (ThermoFisher) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... the cell nucleus was stained with 1:1000 DAPI (4’,6’-diamidino-2-phenylindole; Invitrogen; Catalog No: D1306) nuclear stain for 10 minutes at RT ...
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bioRxiv - Systems Biology 2024Quote: ... organoids were cultured in Maintenance Media 2: Maintenance Media 1 supplemented with B27 with vitamin A (Gibco #17504001), instead of B27 without vitamin A ...
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bioRxiv - Biophysics 2024Quote: ... ∼ 1-2 million per mL cells with 0.2 mg/mL Alexa Fluor 488 Dextran (MW 2,000 kDa; ThermoFisher) dissolved in isotonic media were injected into the devices using syringe ...
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bioRxiv - Neuroscience 2024Quote: ... 10% v:v Knockout Serum Replacement [Gibco], 1% v:v 100x Non-essential Amino Acids [Gibco], 2% v:v 50X B27 Supplement [Gibco] ...
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bioRxiv - Molecular Biology 2024Quote: ... as published.62 Nuclei was visualized by 1 μg/ml 4′,6-diamidino-2-phenylindole (DAPI, from ThermoFisher) as published.23
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bioRxiv - Immunology 2024Quote: ... Tconv cells (2 × 104 cells/well) were stained with the fluorescent dye CFSE at 1 μg/ml (Invitrogen). Flow cytometry analyzing CFSE dilution was performed by gating on CFSE+ cells stimulated for 72 hours in round- bottomed 96-well plates (Corning Falcon ...
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bioRxiv - Immunology 2024Quote: ... genes encoding MD39 HIV Env gp140 were cloned into pHLsec by Genscript and co-transfected with human furin in a pcDNA3.1 plasmid using a 2:1 trimer:furin DNA ratio with polyethylenimine into FreeStyle 293-F cells (ThermoFisher) and incubated for 6 days ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted 1:4 and 2 μL was used in a 20 μL qPCR Taqman assay (Invitrogen). Assay probes were used to detect cDNA from Nkx2.9 (Mm00435145_m1) ...
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bioRxiv - Physiology 2024Quote: ... to identify cytoplasmic F-actin fragments and 4’,6-diamidino-2-phenylidole (DAPI) (Invitrogen Cat. #D1306; 1:1,000) to label nuclei ...
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bioRxiv - Neuroscience 2024Quote: ... A488 rabbit anti-GFP (1:1000, for 2 hours at room temperature, Molecular Probes Cat# A-21311, RRID:AB_221477). Tissues were washed thrice in 1X PBS and incubated at room temperature for 2 hours in the appropriate secondary antibodies (1:1000 in 5% NGS in 0.25% PBST) ...
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Placentas from SARS-CoV-2 infection during pregnancy exhibit foci of oxidative stress and DNA damagebioRxiv - Microbiology 2024Quote: ... Sections were incubated overnight at 4℃ with primary antibodies specific for SARS-CoV-2 Nucleocapsid (1:300, Invitrogen), Spike (1:500 ...
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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activitybioRxiv - Bioengineering 2024Quote: ... Undeveloped photoresist was washed off with SU-8 developer (1-methoxy-2-propanol acetate, Fisher Scientific, Waltham, MA).
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bioRxiv - Immunology 2024Quote: ... or Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco, 1 bead:2 Tresp), in RPMI 1640 medium (Nacalai Tesque ...