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Citations for Thermo Fisher :
8601 - 8650 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... 7-Aminoactinomycin D (7-AAD) (1:200 dilution, ThermoFisher Scientific #A1310 ...
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bioRxiv - Neuroscience 2023Quote: ... was dissolved in 1 mL dimethyl sulfoxide (DMSO, Fisher Scientific) and adjusted to a final concentration of 0.5 mg/ml CNO in 7.5% v/v DMSO in sterile saline ...
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bioRxiv - Immunology 2022Quote: ... 5% Blotting Grade Blocker, Bio-Rad cat. # 1706404 in PBS-T or F: 2% Blotting Grade Blocker and 2% goat serum, Gibco, cat. #16210-072 in PBS-T). Blocking buffer was removed by washing plates three times with PBS-T on an EL406 plate washer ...
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bioRxiv - Developmental Biology 2021Quote: ... Single-cell suspensions <40μm were cryogenically stored in 5μM StemMACS™ Y27632 90% heat-inactivated fetal bovine serum (Gibco)/10% dimethyl sulfoxide (Invitrogen). For each placenta ...
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bioRxiv - Neuroscience 2023Quote: ... DMSO (dimethyl sulfoxide; Invitrogen) was added to dissolve the formazan formed and the absorbance of this solution was measured ...
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bioRxiv - Neuroscience 2023Quote: ... dimethyl pimelimidate (DMP, ThermoFisher), bis-(Sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2023Quote: ... Dimethyl dithiobispropionimidate (DTBP) (ThermoFisher) at 37°C for 15 min ...
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bioRxiv - Immunology 2022Quote: ... in the presence of T cells at a 1:1 effector:target cell ratio in the presence of the caspase-3/7 Green Detection Reagent (Invitrogen). Images were taken every 2 h at 10× magnification ...
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bioRxiv - Neuroscience 2022Quote: ... and their CA1 region bolus-stained with the Na+ sensitive dye SBFI-AM (sodium-binding benzofuran isophthalate-acetoxymethyl ester; Invitrogen, Karlsruhe, Germany). SBFI was alternatively excited at 340/380 nm at an imaging frequency of 0.5 Hz and emission was collected >440 nm from defined regions of interest (ROIs ...
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bioRxiv - Cancer Biology 2021Quote: ... in medium containing active caspase-3/7 detection reagent (ThermoFisher). Chamber slides were mounted on a heated stage within a temperature-controlled chamber maintained at 37°C ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with CellEvent Caspase-3/7 Green (Invitrogen). The cytotoxicity was assessed by flow cytometry as the percentage of Caspase 3/7+ cells in the target cell population ...
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bioRxiv - Immunology 2022Quote: ... stained for apoptosis with CellEvent Caspase-3/7 (Thermo Fisher), incubated for additional 30 min ...
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bioRxiv - Pathology 2022Quote: ... cells were stained for activated caspase 3/7 kit (Invitrogen); 7-amino-actinomycin D (7AAD ...
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bioRxiv - Cancer Biology 2024Quote: ... CellEvent Caspase-3/7 Green ReadyProbes™ Reagent (Invitrogen, R37111) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... a Caspase-3/7 assay kit was used (Invitrogen, C10427). Each tube was brought to a volume of 1 ml staining buffer and 1 μl of CellEvent Caspase-3/7 reagent was added ...
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bioRxiv - Cancer Biology 2022Quote: ... CellEvent Caspase-3/7 Green Flow Cytometry Kit (Thermo Fisher) was used to measure fraction of apoptotic cells by flow cytometry (BD LSR Fortessa with HTS sampler) ...
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bioRxiv - Biophysics 2023Quote: ... 7-Diethylamino-3-(4’-Maleimidylphenyl)-4-Methylcoumarin (CPM) (Invitrogen. USA), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC ...
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bioRxiv - Cancer Biology 2023Quote: ... Invitrogen CellEvent Caspase-3/7 green detection reagent (C10423, ThermoFisher) was used as stated in manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... 60 μM caspase 3/7 red detection reagent (Thermo Fisher), and the pan-caspase inhibitor Z-VAD-fmk (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON).
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bioRxiv - Biochemistry 2024Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher) was added and apoptotic cells were detected with Leica DMi8 fluorescence microscope (Leica Microsystems ...
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bioRxiv - Cell Biology 2024Quote: ... we changed the medium to a 1:1 mixture of KSR and N2B medium (DMEM F12, Thermo Fisher 11320033, with 1% GlutaMAX, 3% dextrose, N2-Supplement B, StemCell Technologies 07156, 5 μg/mL puromycin, Life Technologies A11138-03, and 2 μg/mL doxycycline). On day three ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were spiked-in with the ERCC spike-in controls (2 μL/sample, diluted 1:100, Invitrogen #4456740), treated with on-column DNase digestion according to manufacturer’s specifications (Qiagen) ...
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bioRxiv - Biophysics 2020Quote: ... supernatant was removed and cells were incubated with 1 μg of Fura-2 AM dye (Thermo Scientific, USA) at 1mg/ml DMSO in a total volume of 100µl for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... and probed for 2 h with an FITC-conjugated goat anti-rat antibody (1:300, Thermo Fisher Scientific). VE-cadherin in the superficial vascular plexus and deep capillary plexus was visualized in the retinas by confocal microscopy as illustrated in Fig ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were expressed in 1-2 liters of SF900 III SFM media (Thermo Fisher Scientific, Waltham MA) set at 7 × 105 cells/ml in 2.8L or 5L Optimum Growth Flasks (Thomson Instrument Company Oceanside ...
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... 2% vol/vol FBS and 1% vol/vol antibiotic-antimycotic (#15240062, Gibco, Thermo Fisher Scientific, Waltham, MA, USA) referred to hereafter as HBSS+ ...
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bioRxiv - Bioengineering 2020Quote: Panc-1 and MiaPaCa-2 cells were homogenized in a radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher Scientific, #89901) containing protease and phosphatase inhibitors (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Parasite nuclei were visualized by incubating samples with 1-2 µg/ml Hoechst 33342 (Thermo Scientific Pierce 62249) for 10-20 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... or Alexa 647-labeled species-specific secondary antibodies for 2 hr at a dilution of 1:200 (Invitrogen; Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C). Also ...
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bioRxiv - Neuroscience 2021Quote: ... buffered with 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... with SARS-CoV-2 N-specific primers (EV Table 1) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was performed in parallel using purified SARS-CoV-2 viral RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... at 37°C for 1 minute and further dissociated in digestion medium containing 2 mg/ml Dispase (Invitrogen) and 0.1 mg/ml DNase I (Worthington ...
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bioRxiv - Cell Biology 2021Quote: Total RNAs were extracted from N2 and daf-2(e1370) worms on adult day 1 using TRIzol (INVITROGEN), followed by using DNase I to remove contaminant DNA ...
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bioRxiv - Cell Biology 2021Quote: ... for 1–2 h at 37°C followed by a 10 min incubation with Accutase (Thermo Fisher Scientific) at 37°C to obtain a single cell suspension ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h with donkey anti-rabbit Alexa Fluor488 (1:200, ThermoFisher Scientific, A-21206) in 1% FBS PBS-Tx ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated for 2 hours at RT with streptavidin Alexa488 conjugate (S11223, Molecular Probes, dilution 1:500). Washings ...
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bioRxiv - Microbiology 2021Quote: ... As secondary Ab were used: goat anti-mouse F(ab)’2 AlexaFluor 488 (Molecular Probes, A11017, 1:300) and goat anti-rabbit Cy3 (Jackson Laboratories 111-166-003 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The electroporated cells were immediately transferred to 2 mL microfuge tubes with 1 mL of SOC media (Invitrogen), grown at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% Triton X-100) containing fresh protease inhibitors (0.1% 2-mercaptoethanol, 1 mM phenylmethylsulfonyl fluoride (PMSF; ThermoFisher Scientific), 1 mM benzamidine hydrochloride ...
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bioRxiv - Cell Biology 2021Quote: ... the tissues were mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA, Sigma R1753, 2× SSC, 0.02% BSA, Ambion AM2616 ...
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bioRxiv - Cell Biology 2020Quote: ... for 5 min and mounted with the hybridization buffer (10% Dextran sulfate Sigma D8906, 20% Formamide, 1 mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded with 2×105 density in a 1 mL medium in 12-well plates (ThermoFisher Scientific). After 24-hour incubation ...
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bioRxiv - Immunology 2022Quote: ... Total cDNA was generated from 1-2 µg of RNA using random hexamer primers and Revertaid RT (Thermofisher). Taqman qPCR was used to quantify LT mRNA levels with normalization to TATA-Box Binding protein and compared to a standard curve to determine copy number (Maru et al. ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml of the seed virus was treated with 2 μl TURBO DNase (Thermo Fisher Scientific, Cat# AM2238) and incubated at 37°C for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Hybridization mix contained hybridization buffer (10% Dextran sulfate Sigma D8906, 30% Formamide, 1□mg/ml E.coli tRNA Sigma R1753, 2× SSC, 0.02% BSA Ambion AM2616 ...
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bioRxiv - Microbiology 2022Quote: The Spike gene sequence from the severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1 (NC_045512.2) was codon optimized (GeneArt, ThermoFisher) and gene blocks with overlapping sequences were synthesized by Bio Basic Inc (Markham ...
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bioRxiv - Microbiology 2022Quote: ... Fifty μL of 1:3000 diluted mouse anti-SARS-CoV-2 Spike Protein S1 monoclonal antibody (GT623, Invitrogen) in 1% BSA-PBS blocking buffer was added and incubated at room temperature for 30 min ...