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Citations for Thermo Fisher :
8551 - 8600 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 1% w/v BSA) were precomplexed with anti-human Fc specific antibodies-HRP (A18817, ThermoFisher Scientific, 2:1 ratio lectin:antibody) and pre-incubated with 20 mM EDTA or 40 mM glucose ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were dissociated to single cells with Accutase supplemented with 10 µM Y-27632 and replated in neural maintenance medium (Neurobasal Plus, supplemented with 2% B27+ supplement, 1% Pen/Strep, 1% GlutaMax (all ThermoFisher) supplemented with 10 ng/mL BDNF and 5 ng/mL GDNF (both R&D Systems) ...
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bioRxiv - Biochemistry 2024Quote: ... pH 7.4, 10 mM KCl, 2 mM MgCl2, 1 mM EDTA, 1 mM EGTA with protease and phosphatase inhibitors Thermo Scientific, Waltham ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were injected into a C-18 trap column (Acclaim PepMap100, 75 μm i. d. x 2 cm, C18, 3 μm, 100 Å, Thermo Fisher Scientific) and further separated on a C-18 analytical column (Acclaim PepMap100 ...
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bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Developmental Biology 2021Quote: Embryos and larvae from 9 hpf to 4 wpf were incubated in 3 µM 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Scientific, cat#: C10639) in FSW for 15-30 min ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with 100 ng/ml LPS for 3 hr followed by incubation with 2 µM Mitosox red (Thermo Fisher Scientific, M36008) for 30min ...
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bioRxiv - Molecular Biology 2021Quote: ... They were maintained on irradiated mouse embryonic fibroblast feeder cells (p2 DR4 expanded MEFs from WT-MRC Cambridge Stem Cell Institute) and were passaged every 2-3 days using TrypLE (Thermo Fisher Scientific, 12605028). ROCK inhibitor (10μM ...
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bioRxiv - Cancer Biology 2020Quote: ... an aliquot of each enriched sample was injected onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, 3 μm, 100 Å, Thermo Scientific) coupled to an analytical column (EASY-Spray PepMap column ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from 2×106 cells (3 biological replicates for wild-type and CNOT3-DM cells) using Trizol reagent (Thermo Fisher Scientific, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... and loaded onto a reverse phase trap column (Acclaim PepMap 100, 75µm x 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, U.S.A.) using an Ultimate 3000 for 50 µL at a flow rate of 10 µL min-1 ...
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bioRxiv - Immunology 2021Quote: ... Peptides were initially trapped on a 2 cm long trap column (Acclaim PepMap100, 3 μm C18 particle size, 100 Å pore size, 75 μm inner diameter, Thermo Fisher Scientific) for 10 min at a flow rate of 6 μl/min with 0.1 % (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid ...
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bioRxiv - Plant Biology 2021Quote: ... Five microliters of peptide mixtures were loaded onto an Acclaim PepMap™ precolumn (75 μm × 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm × 50 cm ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Biophysics 2021Quote: ... cells were incubated in blocking buffer with 8 ng/μL of custom labeled secondary antibodies or of commercial IgG-gam-F(ab’)2-Alexa Fluor 647 (DOL ~ 3) (Thermo Fisher, A-21237) for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were harvested and fixed with 3 % PFA for 15 minutes and then stained with 2 μM of CellTracker™ fluorescent probes (ThermoFisher Scientific #C34552). Z-stacks were taken using a Leica TCS SP5 Matrix confocal microscope with the 20X objective ...
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bioRxiv - Cell Biology 2020Quote: Fibroblasts were plated in 96-well plates (10,000 fibroblasts/well) for 3 days in 2% FBS in DMEM supplemented with ascorbic acid (50 μg/mL) (Fisher Scientific, Waltham, MA, USA). Fibroblasts and matrix were then fixed and immunostained as detailed above ...
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bioRxiv - Cell Biology 2022Quote: ... siRNA oligonucleotides were obtained as pre-designed siRNAs as follows: MFF-sense strand: 5’-CGCUGACCUGGAACAAGGAdTdT-3’ for exon 2 30 (Ambion, Austin, TX, USA); DLP1-sense strand ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were loaded onto an Acclaim Pepmap 100 C18 trap column (75 μm x 2 cm nanoViper, 3 μm, 100 Å) (Thermo Fisher Scientific) at 2 μL/min ...
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bioRxiv - Neuroscience 2022Quote: ... free floating NAc sections were first washed (3 × 5 min) in 1x PBS containing 2% Triton X-100 (PBST) (Thermo Fisher, Waltham, MA). Sections were then blocked in 5% normal goat serum (NGS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... through PCR amplification using pair of primers 2 and 3 (Table S1) with a 2X Phusion Flash PCR Master Mix (Thermo Scientific, Cat. # F548S). Gibson Assembly (New England Biolabs ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried peptides were resuspended with 0.1%FA and 500ng peptides were loaded on a HPLC-column (75-μm inner diameter, 2-cm length, 3-μm particle size, 100-Å pore size, Thermo Fisher Scientific) at a flow rate of 2 μL/min ...
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bioRxiv - Microbiology 2020Quote: ... The peptide samples were loaded (2 µl) onto a C18-reversed phase Acclaim PepMap 100 trap column (2 cm x 75 µm, 3 µm particles; Thermo Fisher Scientific, USA) with 30 µL of buffer A (2% v/v acetonitrile ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptide mixtures were loaded on a C18 Acclaim PepMap100 trap column (75 μm inner diameter × 2 cm, 3 μm, 100 Å; Thermo Fisher Scientific) for 3.5 min at 5 μL/min with 2% acetonitrile (ACN) ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated at 4°C for 3 hours on a rotator before being placed in a DynaMag™-2 magnetic rack (Thermo Scientific, 12321D) and washed twice with TBS+NP40 wash buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm x 2 cm, C18, 3 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL min−1 for 3 min ...
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E-cadherin regulates the stability and transcriptional activity of β-catenin in embryonic stem cellsbioRxiv - Cell Biology 2021Quote: ... Cells were replated at a density of 2×103 cells/cm2 every 3 days after dissociation with 0.25% trypsin–EDTA (1X) (Gibco, cat no. 25200-056). Cells were grown in Knockout DMEM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... An adenylated adaptor was ligated to the 3’-end of the captured transcripts (Supplementary Table 3) by using a mixture of T4 RNA ligase and truncated T4 RNA ligase 2 (ThermoFisher Scientific and NEB), which served as a template for the reverse transcription primer (Supplementary Table 3) ...
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bioRxiv - Genetics 2021Quote: ... Five microliters of peptide mixture were loaded onto an Acclaim PepMap™ precolumn (75 μm x 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm x 50 cm ...
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bioRxiv - Molecular Biology 2022Quote: ... TFA as mobile phase on a trap column (Acclaim PepMap100, 2 cm length, 3 μm C18 particle size, 100 Å pore size, 75 μm inner diameter, Thermo Fisher Scientific) and finally separated at 60 °C using a 2 h gradient from 4 to 40 % (v/v ...
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bioRxiv - Immunology 2023Quote: ... The larvae were washed 2 additional times with 40 mls of sterile water followed by 3 washes with 40 mls of sterile PBS (Gibco™ PBS [10X]). Larvae were counted under a dissecting microscope using a McMaster slide and stored in vented U-shaped 275 ml tissue culture flasks (Polystyrene tissue culture flasks with vented caps ...
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bioRxiv - Cell Biology 2023Quote: Aliquots of each sample were loaded onto a trap column (Acclaim® PepMap 100 pre-column, 75 μm × 2 cm, C18, 3 μm, 100 Å, Thermo Scientific) connected to an analytical column (EASY-Spray column ...
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bioRxiv - Neuroscience 2023Quote: ... A full media change was performed every 2-3 days and cells were split by dissociation with TrypLE Express Enzyme (Gibco, cat no: 12605010).
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bioRxiv - Biochemistry 2024Quote: ... The samples were pre-concentrated and desalted online using a PepMap C18 nano-trap column (length - 2 cm; inner diameter - 75 μm; particle size - 3 μm; pore size - 100 Å; Thermo Fisher Scientific) with a flow rate of 3 μL/min for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... The growth medium was changed every 2 – 3 days and consisted of RPMI-1640 (for the RIN14B cells) (Fisher Scientific, 11-875-093) or otherwise DMEM/F12 (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were first trapped for 3 min on a C18-reverse phase trapping column (Acclaim PepMap® 100, 75 μm x 2 cm, particle size 3 μM, nanoViper, Thermo Fisher Scientific), followed by separation on a C18-reverse phase analytical column (Acclaim PepMap® 100 ...
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bioRxiv - Bioengineering 2023Quote: ... The plates were blocked by 3% BSA/PBS for 2 h and then incubated with serial dilutions of an EPO standard (Thermo Fisher Scientific, USA)) or culture supernatant samples for 4 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from the LV myocardium of 2-month-old male WT and Rbm20ΔRS mice (n = 3 per group) using TRIzol Reagent (Life Technologies, Waltham, MA, USA) and treated with DNase I (cat# D9905K ...
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bioRxiv - Molecular Biology 2023Quote: ... concentrated and washed for 3 min on a C18 reverse phase precolumn (3 μm particle size, 100 Å pore size, 75 μm inner diameter, 2 cm length, from Thermo Fisher Scientific). Peptides were separated on an Aurora C18 reverse phase resin (1.6 μm particle size ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 % SDS) for 30 minutes at 50 °C prior to reprobing using mouse anti-3-phosphoglycerate kinase anti-serum (Invitrogen-Molecular probes, A6457).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Females were then primed with subcutaneous administration of estradiol benzoate (17-β-Estradiol-3-Benzoate, Fisher Scientific, 2 μg dissolved in sesame oil) for the 3 days preceding any days on which they were recorded.
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bioRxiv - Evolutionary Biology 2024Quote: ... or 150 heads or tails 3 hours post amputation (hpa) were transferred to a 2 mL tissue grinder (Fisher Scientific, 50-212-708) and washed with artificial seawater ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were loaded onto a pre-column (Acclaim PepMap 100, 75 µm x 2 cm, C18, 3 µm and 100 Å particles, Thermo Fisher Scientific) before being separated over a 25-cm IonOpticks Aurora Series column (IonOpticks) ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were washed with PBST 3×2 minutes then counterstained with a fluorophore conjugated variation of wheat germ agglutin (Invitrogen; W11261; 10ug/mL), phalloidin (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: Samples were loaded onto Acclaim PepMap 100 C18 column (75 μm × 2 cm, 3 μm particle size and 100 Å pore size; #164946, Thermo Fisher Scientific) and separated using nano-capillary C18 column ...
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bioRxiv - Biophysics 2021Quote: ... and HA-mUNC79 plasmids or their various mutants (in a modified BacMam expression vector 27) at a ratio of 2:1:1:1 using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 24 hours before recording ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for overnight with primary antibody at 4 degrees and further incubated with secondary antibodies (1:500) for 1 hour followed by 4′,6-diamidino-2-phenylindole (DAPI) or phalloidin 488 (1:400, Thermo Fisher, A12379) staining ...
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bioRxiv - Cell Biology 2022Quote: HUVECs expressing PIK3CA p.H1047R or PIK3CA wt were transfected with a Silencer Select siRNA targeting to TGFA (ID: s14053) or negative control siRNAs (#1 and #2 mixed in ratio 1:1; all siRNA oligonucleotides from Thermo Fisher Scientific) as previously described (58) ...
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bioRxiv - Physiology 2023Quote: ... Cells were grown on DMEM/Hams F12 with 2% fetal bovine serum and standard cell culture hormones (Dexamethasone 1 1 mM; mM, T3 1 μM, ITS 100X Gibco cat # 41400045) on 6-well ...