Labshake search
Citations for Thermo Fisher :
8801 - 8850 of 10000+ citations for 7 Chloromethyl 2 2 dimethyl 2 3 dihydro 1 benzofuran since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... washed 2 x 2 min with 1X PBS, and incubated with anti-rat 546 (1:500, Thermo Fischer) and DAPI (1:10000, Life Technologies) for 30 min at RT ...
-
bioRxiv - Bioengineering 2024Quote: ... A total of 4 μg of protein were extracted from each lysate and reduced with 1:1 dilution of 2-mercaptoethanol (Thermo Scientific) and 2× Laemilli Buffer (Biorad) ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 1 mL of Krebs solution with calcium containing 1 μM of the fluorescent mitochondrial indicator Rhod-2 AM (Invitrogen, Switzerland) for 1 h at RT ...
-
bioRxiv - Developmental Biology 2024Quote: ... The culture media for CD34+ cell support following isolation contained DMEM and F12 (2:1 ratio) supplemented with 1% antibiotic/antimycotic (ThermoFisher Scientific), 20 mg/mL ascorbic acid ...
-
bioRxiv - Neuroscience 2022Quote: ... and their CA1 region bolus-stained with the Na+ sensitive dye SBFI-AM (sodium-binding benzofuran isophthalate-acetoxymethyl ester; Invitrogen, Karlsruhe, Germany). SBFI was alternatively excited at 340/380 nm at an imaging frequency of 0.5 Hz and emission was collected >440 nm from defined regions of interest (ROIs ...
-
bioRxiv - Genetics 2023Quote: ... neuronal rosetes were passaged as small clusters in either a 1:1 or 1:2 ratio using the StemPro™ EZPassage™ Disposable Stem Cell Passaging Tool (Gibco, #23181010) or via hand-picking ...
-
bioRxiv - Bioengineering 2023Quote: ... then incubated at room temperature in the dark for 2 h with secondary antibodies (AlexaFluor 488 goat anti-mouse IgG 1:400 or 1:600) or rhodamine phalloidin (Invitrogen, R415, 1:600) to visualize F-actin ...
-
bioRxiv - Cell Biology 2020Quote: ... Grids were blotted for 2-3 seconds before plunge freezing in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA, USA) at 4 °C and 100% humidity.
-
bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were seeded onto polyethylenimine-coated 3-cm dishes (2 × 105 cells/dish) and cultured in neurobasal medium (Gibco; Thermo Fisher, Waltham, MA, USA) supplemented with B-27 and 2 mM Glutamax (Gibco ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2021Quote: ... proteins were digested with both trypsin and chymotrypsin and the resultant peptides were loaded onto a reverse phase C18 trap column (Acclaim PepMap 100, 75 μm x 2 cm, nano viper, C18, 3 μm, 100 Å, ThermoFisher, Waltham, MA, U.S.A) using an Ultimate 3000 and washed with 50 μL of solvent A at 10 μl/min ...
-
bioRxiv - Neuroscience 2020Quote: ... CO slices were perfused at a rate of 2-3 mL/min in oxygenated MitoTracker staining solution containing: ACSF and MitoTracker™ Red CMXRos (Invitrogen™, M7512, 100nM) for 30 minutes at 29–32°C in the dark ...
-
bioRxiv - Cell Biology 2021Quote: ... We isolated neonatal cardiomyocyte (postnataTl 2 – 3 days) from Gja1M213L/M213L and Wildtype (WT) mice using PierceTM Primary Cardiomyocyte Isolation Kit (Thermo Fisher Scientific, Walthman, MA) following manufacturer protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... and IVH-PHH (Supplementary Figure 2) P7 rats underwent intraventricular injection of 20 µl of 3 kDa Red Dextran Tetramethyl rhodamine (RD/TMR) (D3307, Thermo Fisher Scientific, Waltham, MA), 1.9-nm (1102 ...
-
bioRxiv - Physiology 2022Quote: ... Brains were quickly transferred to 10 μL of the same saline in a 200 μL microcentrifuge tube kept at 2-3°C in a Boekel Scientific TropiCooler Benchtop Incubator (model 260014, Fisher Scientific, Ottawa, ON, Canada), and once 30 brains were collected (2-2.5 min per brain ...
-
bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
-
bioRxiv - Immunology 2024Quote: Vero-CCL81 [for MuV and VSV_RUBV] or Vero-hSLAM [for MeV-IC323] target cells which were seeded at a density of 2 × 104 cells per well in flat-bottom 96-well plates (Fisher Scientific, #08-772-3). The cells were incubated at 37 °C/5% CO2 overnight (∼20 hours) ...
-
bioRxiv - Physiology 2023Quote: ... HBMECs were washed by 2% BSA (3×, 5min) followed by secondary antibody incubation with either Alexa Fluor 488 goat anti-mouse (ThermoFisher Scientific; Catalogue number: A11001) or Alexa Fluor 555 goat anti-rabbit (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... and ∼5-10 µL of peptide was loaded onto a reverse-phase trap column (Acclaim PepMap 100, 75 µm × 2 cm, NanoViper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA, USA) using an Ultimate 3000 autosampler and washed with 40 µL of buffer A at a flow rate of 20 µL min−1 ...
-
bioRxiv - Systems Biology 2024Quote: ... 2-3 butanediol, and erythritol), and organic acids (acetate, succinate, tartrate, citrate, and malate) were determined using an HPLC (Thermo Fisher Scientific, Waltham, MA) equipped with a refraction index and UV/VIS (210nm ...
-
bioRxiv - Immunology 2024Quote: ... differentiated Th2 cells were restimulated with anti-mouse CD3 (4 µg/mL) for 3 hours followed by 2 hours of incubation with monensin (Thermo Fisher Scientific, MA, USA) at 37 °C ...
-
bioRxiv - Pathology 2024Quote: ... and predesigned TaqMan-based qPCR assays for target and housekeeping genes (Table 2) and run on the 7500 Fast Real-Time PCR System and QuantStudio 3 thermocyclers (Applied Biosystems; Waltham, CA, USA). Relative expression values were normalized by the expression of housekeeping genes using the 2-ΔΔCt method ...
-
bioRxiv - Plant Biology 2024Quote: ... Peptides were pre-concentrated on an Acclaim PepMap 100 pre-column (75 µM x 2 cm, C18, 3 µM, 100 Å; Thermo Fisher Scientific, USA) using the loading pump and buffer A (water ...
-
bioRxiv - Bioengineering 2024Quote: ... consisting of a trap column (C18, 75 µm ID x 2 cm, particle size 3 µm pore size 100 Å; Thermo Fisher Scientific, 164946) and an analytical column (75 µm ID x 50 cm ...
-
bioRxiv - Plant Biology 2020Quote: ... The limit and efficiency of each primer pair of both target and reference gene was allowed to measure accurate comparison of gene expression using the 2-ΔΔCT method for relative quantification (the Applied Biosystems User Bulletin No. 2 (P/N 4303859)) (Livak Schmittgen ...
-
bioRxiv - Molecular Biology 2021Quote: ... the input aliquot (100 µl) was mixed with equal volume of 2× protein sample buffer (2× NuPAGE LDS buffer [Life Technologies], 100 mM DTT, 4% [w/v] SDS) and the IP with 100μl 1 x protein sample buffer followed by incubation for 10 min at 75 °C ...
-
bioRxiv - Plant Biology 2020Quote: ... 2 mM DTT before RNA extraction with TRIzol™ Reagent (Thermo Scientific)) ...
-
bioRxiv - Biophysics 2021Quote: ... Cell nucleus were stained with DAPI (4’,6-diamidino-2-phenylindole, Invitrogen) for 10 min at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... U-2 OS cells were detached using TrypLE Express enzyme (Gibco, 12604013) and plated on gelatin (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins were blotted onto nitrocellulose using the iBlot™2 (Thermo Fisher) and developed using LiCor Odyssey CLx Infrared Imaging System.
-
bioRxiv - Cancer Biology 2021Quote: ... resuspended in 2% FBS in HBSS containing 5 g/ml DAPI (Invitrogen), and sorted into DMEM containing 10% FBS ...
-
bioRxiv - Bioengineering 2021Quote: ... Freshly-thawed SARS-CoV-2 was diluted in infection media (DMEM (Gibco) supplemented with 0.1% bovine serum albumin (Sigma) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 2 μl of 10X FastAP buffer (cf = 1X) (Thermo Fisher, #B64) and incubating it at 37°C for 10-15 min.
-
bioRxiv - Cancer Biology 2021Quote: ... After 2 hr incubation with RNase A (Thermo Fisher Scientific, Cat #EN0531) decrosslinked material was purified with 2 x AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Microbiology 2020Quote: ... RBD from SARS-CoV and SARS-CoV-2 were biotinylated (Thermo Fisher) and immobilized on Streptavidin (SA ...
-
bioRxiv - Molecular Biology 2020Quote: Hela CCL-2 and RPE1 (ATCC) cells were maintained in DMEM (Gibco) supplemented with 10% fetal calf serum (Gibco) ...
-
bioRxiv - Cell Biology 2020Quote: ... Nuclei were stained with 5µg/mL 4,6-diamidino-2-phenylindone (DAPI, Invitrogen) and mounted with ProLong Gold Antifade Reagent (Life Technologies) ...
-
bioRxiv - Cell Biology 2020Quote: ... and 10 mM tris(2-carboxyethylphosphine hydrochloride (TCEP; BondBreaker, Thermo Fisher Scientific) for 5 minutes at 99 °C with 1500 rpm shaking followed by 15 minutes sonication in a water bath sonicator ...
-
bioRxiv - Cell Biology 2020Quote: ... and control siRNA (Silencer negative control 2; AM4637) were purchased from Ambion. Approximately 35,000 U2OS cells were added to prewarmed media in 24-well plates or 8-well imaging chambers (ibidi) ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 mM glutamine and penicillin-streptomycin (Life Technologies Corp., Carlsbad, CA, USA). All cells were cultured in humidified incubator with 5% CO2 at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.3% NP-40 in water) with 2% Proteinase K (Invitrogen Cat # 25530049). Lysis reactions were incubated at 55°C for 4 hours ...
-
bioRxiv - Immunology 2021Quote: Human interleukin two (IL-2) Ready-SET Go! ELISA kit (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... Nuclei were labelled with DAPI (4,6-diamidino-2-phenylindole; Life Technologies, D1306) for 3 minutes ...
-
bioRxiv - Cell Biology 2020Quote: ... Cytochrome c oxidase subunit 2 (COX2, MTCO2; A-6404) from Life Technologies and the ND1 (55410-1-AP ...
-
bioRxiv - Cell Biology 2020Quote: HeLa and U-2 OS cells were grown in MEM (Life Technologies) and McCoy’s 5A Medium (ATCC ...
-
bioRxiv - Cell Biology 2020Quote: ... pH7.4) and then incubated for 2-3min in Trypsin/EDTA (#15400054, Gibco) at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... P140508)+ 1 mM sodium pyruvate (Thermo Fisher Scientific)+ 2 mM Glutamax +25mM HEPES + 100Units/ml Penicillin + 100µg/ml Streptomycin (Gibco)+ 57µM β-Mercaptoethanol (Sigma ...
-
bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 spike ORF was synthesised (GeneArt; Thermo Fisher Scientific) with a poxvirus early transcriptional stop sequence (T5NT ...
-
bioRxiv - Developmental Biology 2021Quote: ... CUBIC Reagent 2 was prepared using sucrose (Fisher Scientific, 44% w/w), urea (Sigma ...
-
bioRxiv - Neuroscience 2020Quote: ... Excess primers were removed by adding 2 μl of Exonuclease I (ThermoFisher) mix to each well (1.875 U of ExoI in water ...