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Citations for Thermo Fisher :
8901 - 8950 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... falciparum (3D7, Dd2, and HB3) parasites were cultured in 3-5% human O+ RBCs in RPMI-1640 (Gibco, 110875093) complete medium containing 0.5% Albumax-II (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... Colon was further cut into 5-10 mm pieces and put into a 15 mL falcon tube with 5 mL prewarmed 2 mM EDTA in Hank’s balanced salt solution (HBSS, Gibco) and shaken at 250 rpm for 15 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were trapped on a C18 Acclaim PepMap 100 (5 µm, 300 µm x 5mm) trap column (ThermoFisher Scientific) and eluted onto a C18 Acclaim PepMap100 3 µm ...
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bioRxiv - Immunology 2024Quote: ... comprising 2 μl sample DNA and 48 μl reaction mixture containing per reaction 5 μl PCR buffer 10x (Invitrogen), 2 μl MgCl2 (50 mM) ...
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bioRxiv - Genomics 2024Quote: ... St Jude Children’s Research Hospital) cells were cultured in culture medium F12 media with 2.5% or 5% heat inactivated fetal bovine serum (GIBCO) and Penicillin-Streptomycin (GIBCO) ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed with PBS-0.1%Tríton-X three times (5 minutes each) and incubated with the secondary antibodies (1:2000; Alexa-Fluor conjugated; Invitrogen), at RT for 1h ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA of tooth germs (E15.5 cultured for 5 days) were extracted by using TRIzol™ Reagent (Invitrogen, USA) according to instruction of manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were cultured in 6-well plates at 37 °C in a 5% CO2 atmosphere and split every 72 hours by trypsinization (0.25%, Gibco, ThermoFisher). Stable HEK293 lines were generated by lipofection (Lipofectamine 2000 ...
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bioRxiv - Molecular Biology 2023Quote: The cDNAs encoding MpRBOHB and its variants with 3 × FLAG tag at their 5’-end were cloned into the pcDNA3.1(-) vector (Invitrogen) for the quantitative measurement of ROS in HEK293T cells.
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with secondary goat anti-rabbit antibodies (dilution 1:1000 in TBST/5% milk; Invitrogen, 32460) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in keratinocyte serum-free medium supplemented with 0.05 mg/ml bovine pituitary extract and 5 ng/ml human recombinant epidermal growth factor (Gibco). T24 human bladder cancer cells were cultured in HAM’s F12 medium supplemented with 10% heat-inactivated FBS and 1% penicillin/streptomycin/glutamine (PSG) ...
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bioRxiv - Cell Biology 2023Quote: MDCK strain II cells were cultured at 37°C with 5% CO2 in high-glucose Dulbecco’s Modified Eagle Medium (DMEM, Invitrogen). The medium was supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: ... the media was replaced with Opti-MEM containing Ambion Silencer-Select ERK3 siRNA (5 pmol, catalog number: 4390771 ID: S78451) and Lipofectamine 2000 (2µl, Invitrogen) and cells incubated for 19 h ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from 5×107 promastigotes with the help of the PureLink RNA mini kit (Invitrogen, USA). The RNA was treated with DNaseI (1U DNase I per 2 μg RNA at 37°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were washed briefly with PBS and mounted in 5 µL ProLong Gold Antifade mounting medium (Thermo Fisher Scientific). Cells were imaged at room temperature using a confocal laser scanning microscope (Zeiss LSM800 ...
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bioRxiv - Genetics 2023Quote: ... mice were overdosed with inhaled isoflurane (>5%) and transcardially perfused with 4°C Ca2+/Mg2+-free 1X HBSS (Invitrogen). Bilateral T11-L2 DRGs ...
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bioRxiv - Developmental Biology 2023Quote: Lateral wall explants were immersed for the indicated times at 37°C with 5% CO2 in DMEM-Glutamax (Invitrogen) supplemented with 10% FCS ...
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Immunology 2023Quote: ... IL-5 and IL-13 concentration were assessed following manufacturer’s instructions using mouse uncoated IL-5 and IL-13 ELISA kits (Invitrogen).
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bioRxiv - Immunology 2023Quote: ... cells were suspended in 200 μl of FACS buffer and acquired on Bigfoot 5-laser Spectral Cell Sorter (Invitrogen). Acquired samples were unmixed using Bigfoot Sasquatch software and analyzed with FlowJo software v10.9 (Tree Star Inc.).
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human Embryonic Kidney (HEK)293T cells (ATCC) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (Bodinco and Gibco) ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were maintained in high glucose Dulbecco’s modified Eagle’s medium 5 g/L glucose (DMEM; Gibco, UK, 11965084). Cell culture media were supplemented with 10% (v/v ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cells were kept in culture at 37°C and 5% CO2 and split twice per week by trypsinization using 0.05% trypsin EDTA (Gibco) followed by passage at 1:5 or 1:10 into a new tissue culture (TC)–treated T75 flask (Thermo Scientific ref 156499) ...
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bioRxiv - Microbiology 2023Quote: HEK 293T/17 cells and Vero-E6 cells were cultured at 37 °C and 5% CO2 in growth medium containing high glucose Dulbecco’s modified eagle medium (Gibco) supplemented with 10% calf serum (Hyclone) ...
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bioRxiv - Immunology 2023Quote: ... 100mL cultures of Expi293F cells at a density of 2.5×106 cells/mL were transiently transfected with 50μg each heavy and light chain encoding plasmids and Expifectamine (Invitrogen) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: Total RNA from fly heads was extracted from 5-day-old female flies using the Trizol reagent (Invitrogen, USA), mRNA was purified from total RNA using oligo(dT)-attached magnetic beads ...
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bioRxiv - Neuroscience 2022Quote: Human SH-SY5Y neural cells and HEK-293T cells were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... animals were injected intraperitoneally with 5-ethynyl-2’-deoxyuridine in sterile saline (EdU, Thermo Fisher Scientific, USA, catalog #A10044), at a dose of 50mg/kg mouse on post-MCAO day 3-7 in single daily injections ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were loaded with 5 μM Fura2-AM and 400 ng/mL Pluronic F-127 (Thermo Fisher Scientific, USA) in control external solution for 45 min at 37 °C in 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 5% CO2 and then fixed and permeabilized using the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen, 00-5523). Cells were stained with primary and secondary antibodies as described previously for immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... K18 tau and αSyn fibrils were diluted to 2.5-10 µM in 5 mM acetate buffer (Fisher Scientific, Switzerland) at pH 4.5 and coupled for 750 s at a flow rate of 5 µL/min on all surfaces except for ZD150D ...
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bioRxiv - Neuroscience 2022Quote: ... Background staining was blocked in 5% serum and incubated with either an anti-MHCII (1:500, M5/114.15.2; Thermo Fisher) or a pSer129 (1:5000 ...
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bioRxiv - Neuroscience 2022Quote: Forty micrograms of protein was heated in the loading buffer and loaded on 5–12% gradient NuPage gels (Invitrogen). After the transfer on nitrocellulose membranes ...
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bioRxiv - Genomics 2022Quote: ... The flow-through was centrifuged and resuspended in 5 ml of red blood cell lysis buffer (Invitrogen, 00-4300) for 10 min.
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bioRxiv - Genomics 2022Quote: ... The flow-through was centrifuged and resuspended in 5 ml of red blood cell lysis buffer (Invitrogen, 00-4300) for 10 min ...
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bioRxiv - Genomics 2022Quote: ... The fixed cells were centrifuged at 2,500g for 5 min to discard the supernatant and washed by 0.5 mL PBS (Gibco, #20012027) once.
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bioRxiv - Molecular Biology 2022Quote: ... 5 days after electroporation cells were collected and subjected to flow cytometry with an Attune Flow Cytometer (ThermoFisher Scientific). Downstream analysis was performed using FlowJo Software v10.8.2 (FlowJo ...
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bioRxiv - Immunology 2023Quote: ... T-cells were maintained at 37°C in an atmosphere of 5% CO2 in 1640 RPMI media (Life technologies) supplemented with 100 µg ml-1 penicillin (Life technologies) ...
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bioRxiv - Immunology 2023Quote: ... Slides were then washed in 1X PBS three times for 5 minutes and incubated with a secondary antibody (Invitrogen Goat-anti-rabbit 594 ...
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bioRxiv - Biophysics 2023Quote: ... their chest was cut and intra-cardiac injection was performed with 5 ml solution of Dil stain (ThermoFisher D282) to label the luminal side of blood vessels ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were online desalted on a trapping cartridge (Acclaim PepMap300 C18, 5 μm, 300Å wide pore, Thermo Fisher Scientific) for three minutes using 30 uL/min flow of 0.05% TFA in water ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 5 × 105 KP or LLC cells suspended in 450 µl Dulbecco’s phosphate-buffered saline (PBS, Gibco) were injected subcutaneously into the flanks of MPO-KO and WT mice aged 6–14 weeks ...
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bioRxiv - Bioengineering 2022Quote: ... organoids were washed 3 times for 5 minutes each using PBS and mounted on glass microscope slides (Fisher Scientific). 90 μm Polybead Microspheres (Polyscience ...
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bioRxiv - Microbiology 2022Quote: ... the sample was drawn off and the grids were rinsed with 5 μL of nuclease-free water (Invitrogen AM9938). The water was then drawn off and the rinse was repeated ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with Alexa488-labelled goat anti-mouse IgG (A11001, Thermo Fisher Scientific, 5 μg/mL, 45 min, RT), and titres were calculated by the modified Kärber method ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplification reactions were monitored for 5 hours at 30 °C in MicroAmp fast reaction tubes with cap (Applied Biosystems) and using real-time PCR machine (Applied Biosystems).
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bioRxiv - Molecular Biology 2023Quote: ... Human dispersed islets were seeded at a density of 5×104 cells/cm2 on ultra-low attachment plates (ThermoFIsher) and allowed to form spontaneous pseudoislets ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting single cell suspension was washed three time with 5 ml of warm Neurobasal Plus medium (Gibco, A3582901) containing B27 Plus supplement (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... were maintained at 37°C in 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Thermo Fisher), 2 mM Glutamax ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated for 5 hrs before the media was changed to media + 1% Calcein AM stain (Thermo Fisher) and incubated for a further hr ...