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Citations for Thermo Fisher :
8351 - 8400 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 2 μg of each vector were used to transfect 5×105 MC38 cell line with Lipofectamine 2000 (ThermoFisher Scientific) in Opti-MEM medium (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were removed by treatment with 3-5 mL of ACK lysis buffer (Gibco, Cat. No. A1049201) and a subsequent washing step in RPMI 1640 with 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were washed in lysis buffer 5 times and elutes in loading buffer (NuPAGE LDS sample buffer, Thermo Fisher, Cat ...
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bioRxiv - Biochemistry 2022Quote: Cells were dissociated for 5 minutes with either trypsin (0.5% v/v) or an enzyme-free cell dissociation buffer (CDB) (Thermofisher). Although the exact composition is not disclosed by Thermofisher ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from 5 adult flies collected at different time points after Toll activation with TRIzol (Invitrogen). cDNA was synthesized from 1000 ng total RNA using the SuperScript II Reverse Transcriptase kit (Transgene) ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF7 and T47D cells were cultured at 37°C with 5% CO2 in DMEM (Thermo Fisher Scientific, Gibco 41966029) supplemented with 9% FBS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... MCF7 and T47D cells were cultured at 37°C with 5% CO2 in DMEM (Thermo Fisher Scientific, Gibco 41966029) supplemented with 9% FBS (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... and maintained at 37°C and 5% CO2 in 175 cm2 culture flasks using high glucose DMEM (Gibco, ThermoFisher), supplemented with 10% v/v fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... and maintained at 37°C and 5% CO2 in 175 cm2 culture flasks using high glucose DMEM (Gibco, ThermoFisher), supplemented with 10% v/v fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... 5 μl red, green and orange fluorescent beads (of indicated diameter, 20 nm, 100 nm, 200 nm) (Fluospheres, Invitrogen) were added to the mix (from a 1:1000 dilution in water) ...
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Biochemistry 2019Quote: HAP1 cells were grown at 37°C with 5% CO2 in Iscove’s Modified Dulbecco’s medium (IMDM) (Gibco, cat.# sh30228) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2019Quote: ... Detachment of confluent cells was achieved by a 5 minutes incubation in 0,05% Trypsin-EDTA (Trypsin-EDTA 0.25%, 25200-072, Gibco®). Cells were re-suspended and counted using 0.4% Trypan Blue (T8154-20ML ...
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bioRxiv - Neuroscience 2019Quote: ... Cell cultures were maintained in an incubator with 5% CO2 (Forma Series II Water Jacket CO2 Incubator, Thermo Scientific) at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... were maintained in monolayer culture at 37°C and 5% CO2 in one of the following medium: Medium A contained DMEM (4.5 g/L glucose; Gibco) supplemented 100 U/ml penicillin and 100 µg/ml streptomycin sulfate ...
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bioRxiv - Cancer Biology 2019Quote: ... T47D:C4217 cells were grown in 5% charcoal stripped calf serum in phenol red free modified RPMI media (Life Technologies). These cells represent acquired estrogen independence and endocrine therapy resistance in estrogen receptor positive breast cancer ...
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bioRxiv - Cell Biology 2019Quote: ... were grown for 24h on coverslips before being incubated for 30 min with 100µM of 5-ethynyl uridine (EU from ThermoFisher). After washes with PBS ...
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bioRxiv - Microbiology 2019Quote: ... and glycerine (STGG) medium and cultured on Columbia blood agar supplemented with 5% horse blood (PB0122A, Oxoid/Thermo Scientific) and 80μl gentamycin 1mg/mL (G1264-250mg ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were passaged two times a week 1:5 in culture-treated flasks using TrypLE dissociation media (ThermoFisher 12604039).
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bioRxiv - Cell Biology 2019Quote: ... 5 mg protein was incubated with 125 μg α-GFP antibody covalently coupled to M270 magnetic resin (ThermoFisher Scientific) for 1 h with rotation ...
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bioRxiv - Developmental Biology 2019Quote: ... 1:200 dilution for 5 minutes prior to plating and BA2 tissue was soaked in DiI (V22889, Thermofisher Scientific), 1:30 dilution for 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... Enzymes were inactivated by adding 5 ml DMEM/F-12 + 10% HyClone fetal bovine serum (Catalog #SH3091003, Fisher Scientific). Dissociated primary cells were spun down at 1000 rpm for 5 minutes ...
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bioRxiv - Physiology 2019Quote: ... 10 μm thickness muscle cryosections were incubated with 5 μM of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH; Molecular Probes, Eugene) and allowed to dry overnight at room temperature in dark ...
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bioRxiv - Cancer Biology 2020Quote: ... denaturated 5 min at 95°C and finally loaded on NuPAGE 4-12% Bis-Tris protein gels (Life Technologies) and electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Cell Biology 2019Quote: ... Each sample was labeled with 5 µl of a TMT 11-plex reagent (20 µg/µL; A37725, Thermo Scientific) for 90 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Liver-Chips were stained in the upper channel with 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA) (Thermo Fisher) to visualize bile canaliculi and MRP2 activity ...
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bioRxiv - Microbiology 2019Quote: ... 150uL of LB was added in each well with 3uM (final concentration) of DiSC3(5) (Thermo Fisher Scientific, UK) in six U-bottom 96-well plates ...
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bioRxiv - Cell Biology 2019Quote: HeLa cells were grown at 37°C and 5% CO2 in Dulbecco’s Modified Eagle medium (DMEM-Glutamax-I; Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2019Quote: ... Five µL of each sample were loaded on a C-18 precolumn (300 µm ID × 5 mm, Thermo Fisher) in a solvent made of 5% acetonitrile and 0.05% TFA and at a flow rate of 20 µL/min ...
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bioRxiv - Neuroscience 2019Quote: Total RNA from fly heads was extracted from 5-day-old female flies using the Trizol reagent (Invitrogen, USA), subjected to poly(A ...
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bioRxiv - Molecular Biology 2019Quote: ... (5) Streptavidin AF 488 and IdU secondary antibody (goat anti-mouse AF 546, ThermoFisher A-11030; Lot# 1736960, 1904466) diluted 1:50 for 30 min - 1 h ...
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bioRxiv - Biophysics 2019Quote: ... After rinsing with a solution containing NPE-caged ATP (adenosine 5′-triphosphate, P3-(1-(2-nitrophenyl) ethyl) ester) (Invitrogen) (0 to 1 µM ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... EdU (5-ethynyl-2’-deoxyuridine) labelling was performed for 15 min as per the manufacturer’s instructions (C10340, Life Technologies) in order to stain S phase nuclei ...
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bioRxiv - Physiology 2020Quote: ... Membranes were blocked with 5% BSA for 1 hour and western blotted with anti-Myc antibody (Invitrogen; MA1-21316) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 10 × 106 cells were transfected with 20μg of DNA per condition using the Program #5 of the Neon transfection system (Cat. number MPK5000, Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... at 300g for 5 minutes and resuspended in 250μL SP-TALP supplemented with 100μg*mL−1 heparin-fluorescein conjugate H7482 (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... U251 glioma cells were incubated at 37 °C with 5% CO2 and cultured in Dulbecco’s Modified Eagle Medium (GIBCO), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed in digestion buffer by addition of 5 µg Pierce MS grade Trypsin (Thermo Fisher Scientific) at 37°C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... RNAs were ethanol-precipitated and ligated to 5pmol 5’adapters (Table 1) with 10U T4 RNA ligase (Ambion AM2140) supplemented with 12.5% PEG8000 and 2U µl-1 RNasin Plus for 16hr at 16°C before being purified with Oligo Clean & Concentrator ...
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bioRxiv - Bioengineering 2019Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid;D3823, Thermo Fisher Scientific), was added to the culture medium for a duration of 60 min and pumped through the media systems at 80 µl/h via positive pressure provided by a syringe pump ...
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bioRxiv - Cell Biology 2020Quote: ... These duplex reactions were run in triplicate (3 wells) on a QuantStudio 5 Real-Time PCR System (Thermo Fisher). One of two duplex assay pairings were used for each experiment ...
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bioRxiv - Neuroscience 2019Quote: ... Differentiation was induced after 5 days in vitro (DIV) by introducing culture medium containing Neurobasal medium (Invitrogen, MA, USA) supplemented with 1% horse serum (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... 5 µl of total DNA was amplified in a 25µL qPCR reaction containing 10x PCR buffer (Invitrogen®, Germany), 200µM dNTPs ...
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bioRxiv - Microbiology 2020Quote: ... The nuclei were stained with DAPI (1:10,000) diluted in PBS for 5 min and mounted with an antifade reagent (ThermoFisher). Images were acquired with a Leica TCS SP5 confocal microscope system.
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bioRxiv - Cell Biology 2019Quote: ... were cultured at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Plant Biology 2019Quote: ... Rh (10 µg L-1) and Ir (5 µg L-1) in 2% trace analysis grade (Fisher Scientific, UK) HNO3 ...
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bioRxiv - Cancer Biology 2019Quote: ... a using a RP C18 Hypersil Gold (100mm I.D. x 4.6mm, 5 mm 175Å pore size; Thermo Fisher Scientific) in-line with an LTQXL mass spectrometer equipped with a heated electrospray ionization source (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2020Quote: Total RNA was extracted from groups of 5 adults using TRIzol reagent according to manufacturer’s instructions (Invitrogen; 15596–018). The RNA samples were treated with DNase (Ambion ...
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bioRxiv - Developmental Biology 2019Quote: Stock solutions of 5-ethynyl-2’-deoxyuridine (EdU) dissolved in dimethyl sulfoxide were prepared per manufacturer’s instructions (Thermo Fisher). Axolotls (3-6cm tail to snout ...