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Citations for Thermo Fisher :
801 - 850 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... charcoal stripped FCS was used (CS-FCS, Gibco® Life Technologies). The human PCa cell line LAPC4 was kindly provided by Zoran Culig (Innsbruck Medical University ...
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bioRxiv - Cancer Biology 2019Quote: ... charcoal stripped FCS was used (CS-FCS, Gibco® Life Technologies). The human PCa cell line LAPC4 was kindly provided by Zoran Culig (Innsbruck Medical University ...
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bioRxiv - Biochemistry 2022Quote: All recombinant human huntingtin and PRKACA cDNA plasmids were transfected into HEK 293T cells or HTT null HEK 293T cells with Lipofectamine 3000 (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Adherent PB2-expressing HEK-293T (HEK-293T-PB2) cells (41) were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, #41966029) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK-Lucia RIGI and HEK-Dual TLR3 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies, Waltham, MA) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Bioengineering 2023Quote: ... pRSV-Rev = 5: 3: 2) were co-transfected into HEK-293T cells at a ratio of 1:1 using lipofectamine 3000 (Invitrogen, USA, Cat: #L3000015). After 48 h ...
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bioRxiv - Biophysics 2021Quote: ... of Plasmodium falciparum plasmepsin X (XP_001349441) (Fig. 5a) was engineered for expression in a mammalian host (Human Embryonic Kidney cells, HEK cells) by GeneArt (Thermo Fisher, Waltham, MA, USA) and cloned into a variant of pTT5 with the mouse Ig secretion signal peptide on the N terminus and a TEV-cleavable 8x Histidine tag on the C terminus ...
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bioRxiv - Neuroscience 2023Quote: Plasma membrane (PM) fraction was prepared from mouse or human DRG tissues and HEK cells using Mem-PER Plus Kit (Thermo Scientific, Cat No. 89842) and manufacture recommend protocol after biotinylation/streptavidin-magnetic bead (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: HEK-293T cells were cultured in DMEM (Invitrogen) supplemented with 10% heat inactivated FBS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... HEK-GCaMP6s cells were grown in DMEM:F12 (Invitrogen) containing 5% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK 293FT cells were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: HEK-Blue hNOD1 cells (Invitrogen, hkb-hnod1, USA) were grown in DMEM containing 4.5 g/l glucose ...
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bioRxiv - Biophysics 2022Quote: ... into HEK 293T cells with Lipofectamine 2000 (Gibco). Media was harvested after 48 hours ...
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bioRxiv - Biochemistry 2022Quote: HEK-293A cells (R70507, Life Technologies, Carlsbad, CA) and HEK-293T cells (ATCC ...
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bioRxiv - Genomics 2022Quote: ... HEK 293T was cultured in DMEM (Life Technologies) with 10% FBS (Peak Serum) ...
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bioRxiv - Neuroscience 2019Quote: ... HEK cells were grown in DMEM (GIBCO 11995) containing 10% FBS (Thermo Fisher Scientific A3840101) ...
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bioRxiv - Microbiology 2019Quote: HEK cells were grown in a DMEM (Gibco) containing 10% (vol/vol ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293FT cells were obtained from Thermo Scientific. K562 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) ...
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bioRxiv - Cell Biology 2021Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T (ATCC) was grown in DMEM (Gibco) supplemented with 10% FCS (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T (ATCC) were cultured in DMEM (Invitrogen) with 10% FBS and 1% penicillin/streptomycin antibiotics ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293F cells were purchased from Thermo Fisher Scientific company ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293Ts were maintained in DMEM medium (Gibco) supplemented with 100 U/mL penicillin ...
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bioRxiv - Genetics 2023Quote: ... HEK-293T cells were grown in DMEM (GIBCO) containing 10% FBS (VWR Scientific) ...
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Long-term precision editing of neural circuits in mammals using engineered gap junction hemichannelsbioRxiv - Neuroscience 2023Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Genetics 2023Quote: The HEK-293FT cells (Thermo Fisher Scientific, R70007) were cultured to be 70-90% confluent at the point of transfection to produce lentiviruses ...
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bioRxiv - Cell Biology 2023Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% PS ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum and 1% penicillin- streptomycin (Gibco).
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bioRxiv - Immunology 2024Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... we transfected HEK cells using Lipofectamine 2000 (ThermoFisher) with an EGFP cell fill to normalize for transfection as well as the respective KDR LRRTM2 constructs with 1xALFA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293F cells were purchased from Thermo Fisher and grown in FreeStyle™ 293 expression medium (12338026 ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was cloned between 5’ KpnI and 3’ EcoRI sites of the pMT/V5-His vector (Invitrogen). In-frame Tag-RFP-T gene was then introduced at the 3’ end of γ-tubulin gene between 5’ EcoRI and 3’ NotI sites ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting PCR product was then inserted into the 5’ SpeI and 3’ EcoRI sites of the pMT/V5 His-B vector (Invitrogen) containing in-frame mTurquoise2 gene at the 5’ end ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Developmental Biology 2022Quote: ... F 5’-GAACTGTCCAGATGCCCTTCCAGTT-3’ and R 5’-GCATCTGGACAGTTCTGGGAAGCCCG-3’) was cloned by PCR and ligated into the pEF6/V5-His TOPO plasmid vector (Invitrogen). FBLN7-V5-His vector was transfected into CHO cells (FBLN7-CHO ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDs was harvested 96 h after infection ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDwt or RBDmut was harvested 96 h after infection ...
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bioRxiv - Bioengineering 2024Quote: ... and 3 µg of BirA-expressing plasmids were transfected into Expi293 cells using the Expifectamine 293 transfection kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... MiniBAR-GFP sta-ble cell line was transfected with 25 nM of siRNAs targeting luciferase (5’-CGUACGCGGAAUACUUCGA-3’) or human Rab35 (5’-GCUCACGAAGAACAGUAAA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...