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Citations for Thermo Fisher :
1001 - 1050 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: HEK 293T cells were cultured in DEME (Gibco, 11995115) with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... HEK-293T cells were transfected using Lipofectamine 2000 (Invitrogen) for lentiviral expression vector pLL5.0 and third generation packaging constructs pMDLg/pRRE ...
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bioRxiv - Microbiology 2021Quote: ... HEK-293FT cells (R70007) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... HEK-293A cells (R705-07) were purchased from Invitrogen. Murine 10.1 fibroblasts have been described [61] ...
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bioRxiv - Bioengineering 2022Quote: HEK 293T cells were cultured in DMEM (11885, Gibco) supplemented with 10% FBS (Gibco 10082147 ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma):DNA ratio into 70% confluent HEK 293A cells (ThermoFisher) in a T-25 flask.
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bioRxiv - Synthetic Biology 2020Quote: ... HEK-293T cells were obtained from Invitrogen (Carlsbad, CA) as Lenti-X 293 cells for lentivirus production ...
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bioRxiv - Cell Biology 2019Quote: ... HEK-293t cells grown in 6-well plates (Nunc) were transfected with biosensor DNA plus regulator if required ...
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bioRxiv - Microbiology 2020Quote: ... Plasmids were transfected into HEK 293FT packaging cells (Invitrogen) using Lenti-X high-titer lentiviral packaging systems (Clontech) ...
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bioRxiv - Developmental Biology 2022Quote: ... were co-transfected into HEK-293FT cells (Invitrogen R70007), and after 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... HEK-293T cells were transfected using Lipofectamine 3000 (Invitrogen) with 500ng of the psPAX2 ...
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bioRxiv - Cell Biology 2023Quote: ... 1x105 HEK cells were resuspended in media (DMEM, Gibco) with penicillin/streptomycin (0.5× ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293FT (humane embryonic kidney, Invitrogen no. R700-07) cells were cultured the same way ...
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bioRxiv - Immunology 2024Quote: ... The HEK-293FT cell line was purchased from Invitrogen and cultured in DMEM supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2024Quote: Expi293FTM HEK cells (“293F” for short) (Thermo Fisher Scientific) and HEK293 (“AC1P” for short ...
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bioRxiv - Microbiology 2020Quote: ... human lung adenocarcinoma Calu-3 cells (ATCC; HTB-55) in MEM (Gibco; #11095-080) containing 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... primary human foreskin fibroblasts (HFFs) in 3 mL of DMEM (Thermo Fisher Scientific, 11965118) +10% FBS ...
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bioRxiv - Biochemistry 2020Quote: ... Detection and relative quantification of the prevalence of individual glycans was accomplished using the total ion mapping (TIM) and neutral loss scan (NL scan) functionality of the Xcalibur software package version 2.0 (Thermo Fisher Scientific) as previously described (38,39) ...
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bioRxiv - Immunology 2021Quote: ... Equal amounts of heavy and light chain expression plasmids and a 1:3 ratio of PEI was used to transfect 293-6E cells at a density of 1×10^6 cells/mL in Freestyle 293 media (Thermo Fisher Scientific, Cat#: 12338018). Supernatants were collected 6 days post transfection by centrifugation at 4,000g followed by filtration through a 0.22 μM filter (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged ChElp2 was pulled-down with Dynabeads™ His-Tag (Thermo Fisher Scientific, Massachusetts, USA) and the subsequent western blot analyses were performed with anti-His (Sigma ...
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bioRxiv - Cell Biology 2019Quote: Recombinant His-tagged EB1-GFP and His-tagged SEPT5 were transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 0.6-0.8 and induced with 0.2 mM IPTG for 16 h at 18°C for His-tagged-EB1-GFP or cultures were grown to OD600 of 0.4-0.6 and induced with 1 mM IPTG for 5 h at 23°C for His-tagged SEPT5 ...
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bioRxiv - Immunology 2024Quote: ... the lysate supernatant containing His-tagged proteins was affinity-purified with His-Tag magnetic beads (Invitrogen) and used for pull-down assays ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: Flp-In™ T-REx™ 293 cell line (Invitrogen) containing a single stably integrated FRT site and expressing Tet repressor were used for generation of inducible cell lines ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In™ T-REx™ 293 cells (ThermoFisher, R78007) were cultured at 37°C and 5% CO2 in DMEM media with 10% FBS and 1% penicillin-streptomycin and split every 2-3 days before reaching full confluence ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected into 293 package cells with Lipofectamine 2000 (Invitrogen) under DMEM (10% FCS ...
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bioRxiv - Developmental Biology 2019Quote: HEK293 (Flp-In™-293 Cell Line, Thermo Fisher Scientific) were grown in DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... GripTite™ MSR 293 cells (Invitrogen cat no. R795-07) were cultured as per manufacturer’s instructions and were seeded at 3×104 cells per well in a 96 well plate the day prior to starting the assay (24hrs +/- 2 hrs) ...
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bioRxiv - Biochemistry 2021Quote: ... Pure DNA was transfected using ExpiFectamine 293 Transfection Kit (ThermoFisher) using the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... Transfection was performed using ExpiFectamine 293 reagent (Thermo Fisher Scientific). Cell culture supernatant was collected seven days after transfection ...
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bioRxiv - Microbiology 2020Quote: ... Transfection was performed using ExpiFectamine 293 reagent (Thermo Fisher Scientific). Cell culture supernatant was collected three days after transfection and supplemented with 10x PBS to a final concentration of 2.5x PBS (342.5 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... was transiently expressed in FreeStyle 293 expression system (Thermo Fisher). SIRPα-hIg was then purified with Sepharose Protein A/G beads ...
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bioRxiv - Molecular Biology 2021Quote: ... 293 cells were transfected using Lipofectamine 2000 (11668030, ThermoFisher Scientific) with plasmid YTHDC1-FLAG and cell lysates were generated as previously described(Meredith et al. ...
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bioRxiv - Microbiology 2020Quote: ... and maintained in FreeStyle 293 serum-free medium (Thermo Fisher). Two days after transfection approximately 5 × 105 cells were transferred to 96-well plates ...
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bioRxiv - Immunology 2022Quote: ... were transiently transfected in FreeStyle 293-F cells (Thermo Fisher) using Turbo293 (SpeedBiosystems ...
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bioRxiv - Immunology 2020Quote: ... using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific Inc.). Seven days post transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... 80 μg DNA and 216 μl Expifectamine 293 (Thermo Fisher) were prepared in 4 ml OptiMEM ...
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bioRxiv - Systems Biology 2022Quote: ... ExpiFectamine 293 Transfection Enhancer 1 and Enhancer 2 (Thermo Fisher) were added to the cells ...
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bioRxiv - Physiology 2022Quote: ... ExpiFectamine 293 Transfection Enhancer 1 and Enhancer 2 (Thermo Fisher) were added ...
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bioRxiv - Immunology 2022Quote: ... were transfected into GP2-293 cells using Lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... were cultured in Freestyle 293 media (Thermo Fisher, cat#12338026). Vero cells (ATCC CCL-81) ...
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bioRxiv - Microbiology 2020Quote: ... Maxipreped plasmids were transiently transfected into 293-F cells (Thermofisher) and the cells were further cultured in suspension for 6 days before harvesting antibody-containing supernatant ...
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bioRxiv - Molecular Biology 2021Quote: ... in one tube and 53 ul of ExpiFectamine 293 (Gibco) was added to 1 ml of Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... T-REx™-293 cells were purchased from Thermo Fisher Scientific and cultured in MEM GlutaMAX™ supplemented with 10% Tet system approved FBS (Clontech ...
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bioRxiv - Biochemistry 2021Quote: FreeStyle 293-F cells were obtained from Thermo Fisher (R79007), and HeLa (CCL-2 ...
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bioRxiv - Immunology 2020Quote: ... using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific Inc.). Seven days post transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Flp-In T-REx 293 cells were purchased from Invitrogen. HEK293T and Flp-In T-REx 293 cells were cultured in DMEM (Gibco ...
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bioRxiv - Immunology 2021Quote: ... cultured in Freestyle 293 Expression Medium (Thermo Fisher Scientific, USA) were maintained at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Expi293F cultures were transfected with Expifectamine 293 Transfection kits (Invitrogen), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cells were cultured in Freestyle 293 media (Invitrogen, USA) supplemented with Geneticin (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... The media was exchanged with FreeStyle 293 media (Thermo Fisher) prior to the transfection ...