Labshake search
Citations for Thermo Fisher :
651 - 700 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... we used ExpiFectamieTM 293 Transfection Kit (ThermoFisher) and followed the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... using the ExpiFectamin 293 Transfection Kit (Gibco). The transfected cells were collected after 4 days and the supernatant clarified by centrifugation at 10,000g and supplemented with 150 mM NaCl and 20 mM imidazole (final concentration).
-
bioRxiv - Immunology 2023Quote: ... FreeStyle 293 F cells (Thermo Fisher Scientific) were cultured in SMM 293-TII Expression Medium (Sino Biological Inc. ...
-
bioRxiv - Biophysics 2023Quote: ... cultured in FreeStyle 293 medium (Life Technologies) with 2% (vol/vol ...
-
bioRxiv - Cell Biology 2022Quote: ... T-REx™ 293 cells (Invitrogen R71007) were transfected with the PX459 constructs individually in order of ANT3 ...
-
bioRxiv - Biochemistry 2023Quote: T-REx™ 293 cells (Invitrogen R71007) were grown on a plate coated by Cultrex Rat Collagen I (50 µg/ml ...
-
bioRxiv - Bioengineering 2023Quote: ... The ExpiFectamine 293 transfection kit (Life Technologies) was used for transfections as per manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... utilizing the ExpiFectamieTM 293 Transfection Kit (ThermoFisher) as previously reported (56 ...
-
bioRxiv - Immunology 2024Quote: FreeStyle 293-F cells (Thermo Fisher Scientific) were maintained in Life Technologies FreeStyle 293 Expression Medium with GlutaMAX (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2019Quote: ... human 36B4 RT 5′-CCCATTCTATCATCAACGGGTACAA-3′) using Superscript III (Thermo Fisher) at 55 °C for 1 h ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: human TASK-3 was overexpressed by transient transfection (Lipofectamine 2000 (Invitrogen)) into a HEK293 cell line stably expressing AMPK-β-1S108A mutant subunit ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 3 μg/ml of human α-CD3 (Thermo Fisher Scientific), 5 μg/ml of human α-CD28 (Thermo Fisher Scientific) ...
-
bioRxiv - Physiology 2023Quote: CHO-Kv7.2/7.3 and HEK-CaSR were cultured separately in Gibco™ Dulbecco’s Modified Eagle Medium (DMEM)/Nutrient Mixture F-12 (Life Technologies) supplemented with 10% foetal bovine serum (Life Technologies) ...
-
bioRxiv - Immunology 2022Quote: ... All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) and purified as previously described (4 ...
-
bioRxiv - Immunology 2024Quote: ... Human A549 (mCherrypos and Luciferasepos) and HEK-293T tumor cells (in-house stocks) have been cultured in DMEM medium (Thermo Fisher 11995) supplemented like RPMI medium for T cells ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and envelope plasmids into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 according to the manufacturer’s instructions (ThermoFisher Scientific, MA, USA). Cell supernatant containing lentivirus was collected at 24 and 48 hours ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... The resulting cyclic peptide-mRNA:cDNA library was counter-selected 3 times against His Pull-Down Dynabeads (Thermo Fisher), and affinity selected against 200 nM bead-immobilised Retromer for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... we followed the vendor supplied protocol of the His-Pur Ni-NTA Purification Kit (3 ml, Thermo Fisher). The flow through ...
-
bioRxiv - Microbiology 2021Quote: SARS-CoV-2 (438-516) S-RBD((HiS)6 and biotynilated human ACE2 have been purchased from Fisher Scientific (respective references 16534204 and 16545164) ...
-
bioRxiv - Immunology 2023Quote: ... for 3-4 days to OD 0.6 in tryptone soya broth (Oxoid) supplemented with 10% FCS (Gibco) and the antibiotics above ...
-
bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
-
bioRxiv - Biochemistry 2021Quote: ... labeled with 50 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher Scientific Invitrogen Catalog # 12-4998-82) diluted in 1.95 mL TBSF for 10 minutes covered on ice ...
-
bioRxiv - Immunology 2020Quote: ... and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104). Labeled cells were acquired using a Thermo-Fisher Attune NxT flow cytometer and analyzed using Flowjo Software.
-
bioRxiv - Cell Biology 2023Quote: CEM were counted and resuspended at a concentration of 2-3 x 106 cells/mL in ice cold FACS buffer (PBS, 10% FCS, 2mM EDTA) containing 1.25 µL anti-human CD71 (0.625 µg/mL, OKT9, Invitrogen) for 1 h at 4°C then moved to 37°C and incubated for 1 h with or without 100 µM cytochalasin D (Merck) ...
-
bioRxiv - Biochemistry 2022Quote: ... 20 μg of the plasmid was transfected into 20 mL of Expi293F cells at 3 × 106 cells mL-1 using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... Cells grown to a density of 3 million per mL were transfected using the respective plasmids with the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) and cultivated for 5 days ...
-
bioRxiv - Microbiology 2023Quote: ... Expi293F cells were diluted to a density of 3 million cells per mL and transfected using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cells were incubated shaking at 130 rpm at 37°C and 8% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... The pcDNA 3.1+ vectors were then transfected individually into human embryonic kidney (HEK293T) suspension cells using ExpiFectamine 293 transfection reagent (ThermoFisher Scientific, Waltham, MA, USA) following manufacturer’s specifications ...
-
bioRxiv - Immunology 2023Quote: ... post-dose 3 and 6-months post-dose 3 using mouse anti-human IgG1 biotin (Thermo Fisher Scientific) and mouse anti-human IgG4 biotin (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2020Quote: ... (gift from Drs. Steven Balk) and 293T (RRID:CVCL_0063) cells (gift from Dr. Tim Bender) were grown in DMEM (Invitrogen) with 10% heat-inactivated serum ...
-
bioRxiv - Molecular Biology 2021Quote: HEK 293F cells (Thermo Fisher Scientific 12338026) and mAb 2F2 and 2E10.E9 hybridoma cell lines (BEI Resources MRA-184 and -185 ...
-
bioRxiv - Immunology 2019Quote: HEK-293FT cells (ThermoFisher; cat. no. R70007) were cultured in a six-well plate at a concentration of 5 × 105 cells/ml ...
-
bioRxiv - Cell Biology 2021Quote: HEK 293FT cells (Thermo Fisher Scientific R70007) and B16-F1 mouse melanoma cells (ATCC CRL-6323 ...
-
bioRxiv - Cell Biology 2020Quote: HEK cells were cultured in DMEM (Gibco) with 10% heat-inactivated FBS (Gibco) ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK were cultured in EpiLife medium (Gibco), supplemented with 0.6 mM CaCl2 (Gibco) ...
-
bioRxiv - Neuroscience 2020Quote: ... suspension HEK-Expi293F™ cells (ThermoFisher Scientific) were cultured in Expi293™ expression media (Gibco ...
-
bioRxiv - Molecular Biology 2019Quote: 5.5 × 106 HEK 293T cells (Thermo Fisher) attached to 10 cm cell culture dishes (Sarstedt ...
-
bioRxiv - Immunology 2022Quote: HEK-293T was grown in DMEM (Gibco) supplemented with 10% FCS (Gibco) ...
-
bioRxiv - Genomics 2022Quote: ... HEK 293T was obtained from Life Technologies. K562 was authenticated by Short Tandem Repeat profiling (Genetica ...
-
bioRxiv - Genomics 2023Quote: ... HEK 293FT cells were obtained from ThermoFisher Scientific (R70007 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The HEK-293T cells (Thermo-Fisher Scientific) were maintained in Dulbecco’s modified Eagle media (DMEM ...
-
bioRxiv - Systems Biology 2021Quote: ... Tn5 underwent a His-tag (Dynabeads His-Tag Isolation & Pulldown, Invitrogen, 10103D) clean-up according to the manufacturer’s protocol to remove unbound oligos before using.
-
bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
-
bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
-
bioRxiv - Immunology 2020Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
-
bioRxiv - Microbiology 2022Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and His-tag (Invitrogen) were used ...