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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: HEK 293T (HEK) cells were cultured in DMEM (Thermo 11995065) with 10% FBS (Thermo Fisher 10082147) and 1x Pen/Strep/Glutamine (Thermo Fisher 10378016) ...
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293A cells (Thermo Fisher Scientific) were cultured in DMEM with 4.5 g/L D-glucose and GlutaMAX supplemented with 10% (vol/vol) ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293A cells (Thermo Fisher Scientific) were seeded in a 6-well culture plate (Greiner Bio-One ...
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bioRxiv - Bioengineering 2021Quote: HEK 293T cells (Thermo Life Technologies) were cultured in DMEM medium with 10% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... Approximately 200,000 HEK 293FT (Thermo Fisher) cells were seeded on poly-D-lysine and laminin coated co-verslips (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: HEK-293A cells (Invitrogen, Cat#R70507) were maintained at 37°C with 5% CO2 in DMEM (4.5 g/L glucose ...
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bioRxiv - Neuroscience 2022Quote: ... HEK 293T cells (Invitrogen, R700-07) were grown in 15-cm plates (Falcon ...
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bioRxiv - Genomics 2024Quote: HEK-293FT (Thermo Fisher Scientific R70007) and doxycycline-inducible-Cas9 A54957 (a gift from J.T ...
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bioRxiv - Cell Biology 2022Quote: HEK 293FT cells (Thermo Fisher Scientific) were used for all the pulldown experiments unless indicated otherwise ...
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bioRxiv - Cancer Biology 2020Quote: ... envelope and one of the transfer plasmids were co-transfected into human embryonic kidney (HEK 293T) cells using Lipofectamine 3000 (ThermoFisher Scientific) according to the manufacturer’s lentiviral production protocol ...
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bioRxiv - Physiology 2022Quote: ... ATCC) and human HEK-293T cells (catalog no. CRL-3216, ATCC) were grown in DMEM (catalog no. 31966-021, ThermoFisher Scientific) supplemented with 10% Fetal Bovine Serum (catalog no ...
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bioRxiv - Microbiology 2022Quote: ... The human anti-TNFα IgG1 monoclonal antibody was expressed in HEK-293F FreeStyle cell line expression system (Life Technologies, Carlsbad, CA) with co-transfection of vectors encoding p21 ...
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bioRxiv - Molecular Biology 2020Quote: Human embryonic kidney (HEK) 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Thermo Fisher, Waltham, MA, USA; Cat#10566016) supplemented with 10% fetal calf serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Lenti-X Human Embryonic Kidney (HEK) cells were transfected with the sgRNA plasmids using Lipofectamine 3000 (Life Technologies, Cat. No. L3000150), then cultured for 2-3 days in the following media ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding paired Ig heavy and light chains were co-transfected into human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells stably expressing the human D1R (D1-HEK) (up to pass 35) were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Gibco/Fisher Scientific, Hampton, NH) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Cell Biology 2024Quote: Isolated human pancreatic islets and HEK cells were washed and then lysed in Dulbecco’s phosphate-buffered saline (DPBS) (Thermo Fisher Scientific) containing 1% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Human HEK-293T were commercially acquired (cat. no. CRL-11268, ATCC) and grown in Advanced DMEM (cat. no. 12491015, ThermoFisher Scientific) medium supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... supplemented with 10% FCS (FCS, Thermo Fisher Scientific), 100 U ml−1 penicillin ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Biophysics 2024Quote: ... while the N-terminal 6×His-tagged wild-type human Gβ1 and untagged Gγ2 subunits were cloned into a pFastBac-Dual vector (Invitrogen). Recombinant baculoviruses were generated using the Bac-to-Bac® Baculovirus Expression System (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Mouse bone marrow cells were isolated by crushing entire long bones into DMEM/3% FCS (GIBCO). Bone fragments were removed by filtration through a 70 μm filter mesh ...
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bioRxiv - Immunology 2024Quote: ... followed by incubation with 1:5000-diluted anti-human IgG Fc secondary antibody-HRP (Invitrogen, 05-4220) for 1 hr ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Microbiology 2022Quote: ... Cells grown to a density of 3 million cells per milliliter were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and grown for 4 days ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for 3-5 days ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days ...
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bioRxiv - Biochemistry 2023Quote: ... Cells grown to a density of 3 million cells per mL were transfected with the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Immunology 2022Quote: ... Cells grown to a density of 3 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days prior to supernatent harvest ...
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bioRxiv - Biochemistry 2024Quote: ... Expi293F cells were grown to a density of 3 x 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and expression carried out for 3 to 5 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... cells were diluted to 3 × 106 cells/mL in antibiotic-free Expi293 Expression Medium and transfected using an ExpiFectamine 293 Transfection Kit (Thermo Scientific). ExpiFectamine 293 Transfection Enhancers 1 and 2 were added 20 h post transfection and cultures were incubated for 5 days post-transfection ...
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bioRxiv - Immunology 2023Quote: ... cells were grown to a density of 3 × 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific). Three to 5 days post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... Expi293F cells were grown to a density of 3 × 106 cells/mL and transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and expression was carried out for 4 days post-transfection at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... Cells grown to a density of 3 million cells per mL were transfected with the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Developmental Biology 2023Quote: ... Separated tubules were resuspended in TIM with 200 μl of 0.25% trypsin (25200-056, Gibco Chemicals-ThermoFisher) and DNase I (4 μg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... Separated tubules were resuspended in TIM with 200 μl of 0.25% trypsin (25200-056, Gibco Chemicals-ThermoFisher) and DNase I (4 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Both the parent HEK and stable α4β2R HEK cell lines were maintained in DMEM (Gibco, Life technologies) with 10% calf serum (Hyclone ...
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bioRxiv - Immunology 2020Quote: HEK 293T cells and ACE2-transfected HEK 293T cells (ACE2-293T) were cultured in DMEM (Gibco, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Both the parent HEK and stable α4β2R HEK cell lines were maintained in DMEM (Gibco, Life technologies) with 10% calf serum (Hyclone ...
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bioRxiv - Neuroscience 2020Quote: ... Both the parent HEK and stable α4β2 HEK cell lines were maintained in DMEM (Gibco, Life technologies) with 10% calf serum (Hyclone ...
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bioRxiv - Neuroscience 2020Quote: ... Both the parent HEK and stable α4β2 HEK cell lines were maintained in DMEM (Gibco, Life technologies) with 10% calf serum (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293Trex ERGIC53 KO or HEK 293Trex SURF4 KO cells were incubated in Opti-MEM (Invitrogen, NY) containing 10% FBS at 15°C for 2.5 hrs to accumulate cargo proteins at the ER.
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bioRxiv - Microbiology 2022Quote: His-tagged soluble human ACE2 was produced in FreeStyle™ 293-F cells by transfecting soluble human ACE2 (1-741 aa) expression vector plasmid DNA using 293fectin (Thermo Fisher) and purified using HiTrap Chelating column charged with nickel (GE healthcare ...
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bioRxiv - Immunology 2020Quote: FreeStyle 293-F suspension cells were grown in shaker flasks (125 rpm) in Freestyle 293 expression medium (ThermoFisher) in a humidified 37 °C incubator filled with 3% (v/v ...
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bioRxiv - Immunology 2024Quote: ... The 293-F cells stably expressing sCD177 were cultured in the FreeStyle™ 293 Expression Medium (ThermoFisher Scientific) and cell culture supernatant was harvested when cell density reached 2.5×106/ml ...
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bioRxiv - Immunology 2024Quote: ... the blots were probed for the presence of heavy and light chain bands using either horseradish peroxidase (HRP)-conjugated polyclonal goat anti-human IgG Fc antibody or polyclonal goat anti-human kappa chain antibody (Thermo Fisher Scientific, Waltham, MA, USA). Antibody-coupled HRP activity was detected with Supersignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: human TASK-3 was overexpressed by transient transfection (Lipofectamine 2000 (Invitrogen)) into a HEK293 cell line stably expressing AMPK-β-1S108A mutant subunit ...