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Citations for Thermo Fisher :
751 - 800 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... HEK-GCaMP6s cells were grown in DMEM:F12 (Invitrogen) containing 5% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: HEK-293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK 293FT cells were purchased from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: HEK-Blue hNOD1 cells (Invitrogen, hkb-hnod1, USA) were grown in DMEM containing 4.5 g/l glucose ...
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bioRxiv - Biophysics 2022Quote: ... into HEK 293T cells with Lipofectamine 2000 (Gibco). Media was harvested after 48 hours ...
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bioRxiv - Biochemistry 2022Quote: HEK-293A cells (R70507, Life Technologies, Carlsbad, CA) and HEK-293T cells (ATCC ...
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bioRxiv - Genomics 2022Quote: ... HEK 293T was cultured in DMEM (Life Technologies) with 10% FBS (Peak Serum) ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... HEK 293FT cells were obtained from Thermo Scientific. K562 cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) ...
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bioRxiv - Cell Biology 2021Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Cancer Biology 2022Quote: HEK-293T (ATCC) was grown in DMEM (Gibco) supplemented with 10% FCS (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T (ATCC) were cultured in DMEM (Invitrogen) with 10% FBS and 1% penicillin/streptomycin antibiotics ...
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bioRxiv - Genetics 2023Quote: ... HEK-293T cells were grown in DMEM (GIBCO) containing 10% FBS (VWR Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293Ts were maintained in DMEM medium (Gibco) supplemented with 100 U/mL penicillin ...
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Long-term precision editing of neural circuits in mammals using engineered gap junction hemichannelsbioRxiv - Neuroscience 2023Quote: HEK 293FT cells were purchased from Thermo Fisher Scientific (cat# R70007 ...
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bioRxiv - Genetics 2023Quote: The HEK-293FT cells (Thermo Fisher Scientific, R70007) were cultured to be 70-90% confluent at the point of transfection to produce lentiviruses ...
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bioRxiv - Biochemistry 2024Quote: ... HEK-293F cells were purchased from Thermo Fisher Scientific company ...
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bioRxiv - Microbiology 2023Quote: ... HEK-293T cells were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum and 1% penicillin- streptomycin (Gibco).
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bioRxiv - Cell Biology 2023Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 1% PS ...
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bioRxiv - Immunology 2024Quote: ... HEK 293T cells were cultured in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... we transfected HEK cells using Lipofectamine 2000 (ThermoFisher) with an EGFP cell fill to normalize for transfection as well as the respective KDR LRRTM2 constructs with 1xALFA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293F cells were purchased from Thermo Fisher and grown in FreeStyle™ 293 expression medium (12338026 ...
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bioRxiv - Neuroscience 2020Quote: ... for 48 h then transfected for 24 h with 1 ug His-G3BP1 3’UTR constructs using Lipofectamine LTX and Plus reagent (Invitrogen). To determine mRNA stability ...
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bioRxiv - Developmental Biology 2022Quote: ... F 5’-GAACTGTCCAGATGCCCTTCCAGTT-3’ and R 5’-GCATCTGGACAGTTCTGGGAAGCCCG-3’) was cloned by PCR and ligated into the pEF6/V5-His TOPO plasmid vector (Invitrogen). FBLN7-V5-His vector was transfected into CHO cells (FBLN7-CHO ...
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bioRxiv - Biophysics 2020Quote: The DNA sequences of the human CE GTase and each sequence variant were synthesised and subcloned into the PGEX6p1-C-His plasmid vector by Thermo Fisher GeneArt ...
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bioRxiv - Neuroscience 2021Quote: His-tagged human dynamin 1 was expressed using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific, Waltham, MA, USA) and purified as described previously (66) ...
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bioRxiv - Immunology 2021Quote: ... were cloned into pCEP4 mammalian expression vector containing N-terminal human Ig kappa leader sequence and C-terminal Avi-tag and His-tag (Invitrogen, USA). Expi293-Freestyle cells cultured at 37°C and 8% CO2 in growth medium containing Expi293 Expression Medium at 3×106/mL in 50 mL media were transfected overnight at 37 °C with 50μg of plasmid in 160μL of ExpiFectamine plus 6mL of OptiMEM-I (all from Invitrogen) ...
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bioRxiv - Physiology 2021Quote: ... The human full-length BACE1 or BACE2 cDNAs were expressed from the pcDNA3.1/myc-His expression vector (Invitrogen, Waltham, MA, USA).
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDs was harvested 96 h after infection ...
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bioRxiv - Immunology 2021Quote: ... and were then transfected into Expi293F cells at a density of 3×106 cells/ml using ExpiFectatmine 293 transfection kit (Gibco, ThermoFisher Scientific, MA, USA). The supernatant of cell culture containing the secreted RBDwt or RBDmut was harvested 96 h after infection ...
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bioRxiv - Bioengineering 2024Quote: ... and 3 µg of BirA-expressing plasmids were transfected into Expi293 cells using the Expifectamine 293 transfection kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... MiniBAR-GFP sta-ble cell line was transfected with 25 nM of siRNAs targeting luciferase (5’-CGUACGCGGAAUACUUCGA-3’) or human Rab35 (5’-GCUCACGAAGAACAGUAAA-3’) using Lipo-fectamine RNAiMAX (Invitrogen), following the manufac-turer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... His-Tag purification was performed using Dynabeads His-Tag Isolation and Pulldown (Invitrogen, 10103D). Beads were allowed to bind to antibody-pA-Tn5 complex for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
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bioRxiv - Bioengineering 2023Quote: ... The EGFP T-REx 293 cell line used for the mammalian screening was obtained modifying the Flp-In™ T-REx™ 293 Cell Line (Invitrogen) for the expression of inducible EGFP according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and the ELISPOT plate was incubated for 4 hours at 4°C with biotinylated goat anti-human IgG Fc (Invitrogen), followed by incubation for 1 hour at room temperature with horseradish peroxidase (HRP)–conjugated avidin (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2020Quote: Mammalian expression plasmids encoding SARS VHH72 and MERS VHH-55 (73) with a C-terminal a monomeric human Fc tag were transfected into ExpiCHO cells (Thermofisher) using ExpiFectamine as manufacterer’s recommendation ...
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bioRxiv - Developmental Biology 2022Quote: ... These zona-free eggs were incubated with 200 nM Fc-fusion proteins in Biggers-Whitten-Whittingham (BWW) medium for 1 h and then stained with goat anti-human IgG Fc antibody DyLight 488 (Invitrogen) at a dilution of 1:50 for 1 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using goat anti-Human IgG Fc Secondary Antibody conjugated to horse radish peroxidase (Thermo Fisher Scientific), diluted 1:5000 in blocking solution and incubated for 1 hr at RT ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated with a 1:10000 dilution (in PBST with 1% BSA) of goat anti-human Fc HRP-conjugated antibody (Invitrogen) at room temperature for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... and S309 and VHH72-Fc or variants thereof was detected with an AF633 conjugated goat anti-human IgG antibody (Invitrogen). Binding of monomeric VHHs to SARS-CoV-1 or SARS-CoV-2 S was detected with a mouse anti-HisTag antibody (AbD Serotec ...
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bioRxiv - Neuroscience 2020Quote: ... β-neurexin-1 extracellular domain was cloned into the pCAGGS vector with a C-terminal human Fc tag and the ATD regions of GluA1–4 were cloned into a modified pDisplay vector (Invitrogen), as described previously (9) ...
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bioRxiv - Biochemistry 2021Quote: ... The slide was washed thrice with PBS-T for 1 minute and further incubated with goat anti-human IgG (Fc)-Cy5 (Invitrogen) antibodies at 1:2500 dilution ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were then washed and incubated with PE-conjugated goat anti-human Fc antibodies (Thermo Fisher 12-4998-82) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and PToV Hemagglutinin Esterase non-binding (F271A) (1176bp) fused to human IgG-Fc followed by a 6xHis tag in the Insect expression vector pFastBac1 (ThermoFisher) was kindly provided by Colin Parrish (PToV-HE_S46A-Fc_Hisx6/pFastBac1 ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Immunology 2023Quote: ASCs (FVS780-CD20-CD3-CD14-CD16-CD19+CD27hiCD38hi) were sort-purified from human peripheral blood mononuclear cells into culture media (10% FCS, 2 mM L-glutamine (Gibco), 100 units/mL Penicillin and 100 ng/mL Streptomycin (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were then incubated with a 1:10,000 dilution of goat anti-human Fc HRP secondary antibody (Invitrogen, A18817) in PBST for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... Plates were slapped dry and washed 4X in TBST followed by addition of 50 μL 1:5000 Goat anti-Human IgG Fc Secondary Antibody with HRP (Invitrogen) for one hour at 37°C ...