Labshake search
Citations for Thermo Fisher :
8251 - 8300 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, 0.075 × 250 mm (Thermo Fisher Scientific)) with a linear gradient of 4-27.5% solvent B (0.1% FA in 98% ACN ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were imaged in a CM100 transmission electron microscope at 80 keV (Thermo Fisher Scientific, Eindhoven, The Netherlands).
-
bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
-
bioRxiv - Microbiology 2024Quote: ... IFNβ, IFNα and IFIT1) and 15x (for reference genes: ACTIN, RPL13A, YWHAZ and UBC) with Nuclease-free water (Ambion, Life Technologies). 5 μL of the diluted cDNA was then used for qPCR ...
-
bioRxiv - Microbiology 2024Quote: Cell membranes were stained with FM5-95 (1 µg/ml final concentration, Invitrogen) and DNA was stained with DAPI (5 µg/ml final concentration ...
-
bioRxiv - Microbiology 2024Quote: ... and stained with secondary antibody goat anti-mouse AlexaFuor594 (Thermo Fisher Scientific), unless specified ...
-
bioRxiv - Microbiology 2024Quote: ... stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer for 1h at RT and IEDDA-clicked as described above ...
-
bioRxiv - Microbiology 2024Quote: ... cell membranes were stained with Nile red (200 ng/ml, Invitrogen). To reduce background caused by excess membrane dye ...
-
bioRxiv - Microbiology 2024Quote: ... and 10% fetal calf serum (FCS; Gibco). During infection experiments ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA was subsequently diluted 3x (for target genes: IFNβ, IFNα and IFIT1) and 15x (for reference genes: ACTIN, RPL13A, YWHAZ and UBC) with Nuclease-free water (Ambion, Life Technologies). 5 μL of the diluted cDNA was then used for qPCR ...
-
bioRxiv - Microbiology 2024Quote: ... the coverslips were inverted onto a 30- µl droplet of freshly prepared CuAAC click reaction mix [10 µM N3-AlexaFluor647 (Thermo Fisher Scientific), 1 mM CuSO4 ...
-
bioRxiv - Microbiology 2024Quote: ... and genomic DNA libraries were generated according to the manufacturers’ protocols using the Ion Xpress™ Plus gDNA Fragment Library Preparations (Life Technologies). Bar-coded libraries were prepared using the Ion Plus fragment library kit (Life Technologies ...
-
bioRxiv - Microbiology 2024Quote: ... Sequencing was performed on the Ion Proton system using the Ion PI Chips (Life Technologies) according to the manufacturers’ protocols ...
-
bioRxiv - Microbiology 2024Quote: ... coli SM10 were grown in LB broth (Fisher Scientific BP97235) or LB+1.5% agar supplemented when needed with carbenicillin (100 µg/mL ...
-
bioRxiv - Microbiology 2024Quote: ... and HER911 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with non-essential amino acids (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... Quantification of isolated DNA was performed with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA was sent to SeqCenter (Pittsburgh ...
-
bioRxiv - Cell Biology 2024Quote: Raji cells were stained with 1.0 µM Celltracker Deep Red (Invitrogen, C34565) for 30 minutes in supplement-free RPMI1640 at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... pH 7.4) and pre-charged with 25% (v/v) Fluo-4 acetoxymethyl (Invitrogen, # F14201) for 30 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Sections were applied with Prolong Gold Antifade reagent with DAPI (P36935, Thermo Fisher Scientific, USA) and covered with cover glass before proceeding with imaging ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed 2X in PBS and stained with 10 µg/mL Wheat-Germ Agglutinin (WGA) AlexaFluor 647 conjugate (Invitrogen, W32466) for 10 minutes at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... Intracellular Ki67 staining was performed using the FoxP3 transcription factor staining kit per manufacturer protocol (ThermoFisher, 00-5523-00). Cells were fixed in 2% paraformaldehyde for 1 hour at 4°C ...
-
bioRxiv - Cell Biology 2024Quote: ... 10-30 µg of protein was loaded onto precast gel (NP0321, NuPAGE™ 4- 12%, Bis-Tris, Mini Protein Gels, ThermoFisher Scientific, USA) and resolved at 135 volts for 90 minutes ...
-
bioRxiv - Cell Biology 2024Quote: ... Cultured 5637 human bladder cancer cells were lysed in Pierce IP Lysis Buffer (ThermoFisher, 87787), and incubated for 30mins at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... The qRT-PCR reaction was run in QuantStudio™3 Real-Time PCR System (Applied Biosystems™, USA) using the following settings ...
-
bioRxiv - Cell Biology 2024Quote: ... ATF6 (Thermo Fisher, PA5-20216), XBP1 (CST ...
-
bioRxiv - Cell Biology 2024Quote: Bladders of young WT and Ifrd1−/− mice were collected and homogenized in TRIzol™ Reagent (15596026, Thermo Fisher Scientific, USA) to extract total RNA followed by DNase 1 treatment (18068-015 ...
-
bioRxiv - Cell Biology 2024Quote: ... p-PERK (Thermo Fisher, PA5-102853), eIF2α (CST ...
-
bioRxiv - Cell Biology 2024Quote: ... to extract total RNA followed by DNase 1 treatment (18068-015, Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Total protein was quantitated using a BCA assay (Pierce™ BCA Protein Assay Kit, 23225, Thermo Fisher Scientific, USA). 10-30 µg of protein was loaded onto precast gel (NP0321 ...
-
bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was utilized to perform cDNA synthesis using SuperScript™ II Reverse Transcriptase (18064-014, Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... Coverslips were mounted onto slides in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific), let dry over two nights and imaged in a Leica SP8 FALCON cLSM as described.
-
bioRxiv - Microbiology 2024Quote: ... and the Ion Xpress DNA bar coding kit (Life Technologies) according to the 200-base-read Ion Proton libraries instructions of the manufacturer ...
-
bioRxiv - Microbiology 2024Quote: ... Media was replaced with mock or 2.5 µM DFT supplemented FluoroBrite (Thermo Fisher Scientific) at 24 hpi ...
-
bioRxiv - Microbiology 2024Quote: ... medium was removed and samples were clicked with AO-6MT in FluoroBrite (Thermo Fisher Scientific) containing Hoechst 33342 as indicated ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was quantified using a NanoDrop spectrophotometer (Thermo Scientific) and diluted to 5 ng/µL ...
-
bioRxiv - Microbiology 2024Quote: ... supplemented with non-essential amino acids (Thermo Fisher Scientific) and 10% fetal calf serum (FCS ...
-
bioRxiv - Microbiology 2024Quote: ... and stained with secondary antibody goat anti-mouse AlexaFuor594 (Thermo Fisher Scientific), unless specified ...
-
bioRxiv - Microbiology 2024Quote: ... BMDM were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM) GlutaMAX (Gibco #10569-010) supplemented with 1 mM sodium pyruvate ...
-
bioRxiv - Microbiology 2024Quote: ... Bar-coded libraries were prepared using the Ion Plus fragment library kit (Life Technologies) and the Ion Xpress DNA bar coding kit (Life Technologies ...
-
bioRxiv - Microbiology 2024Quote: ... cells were stained with secondary antibody (goat anti-rabbit AlexaFluor488, Thermo Fisher Scientific) diluted in blocking buffer containing 4′,6-diamidino-2-phenylindole (DAPI ...
-
bioRxiv - Microbiology 2024Quote: Membrane potential was measured using the potentiometric fluorescent probe DiSC3(5) (Invitrogen). Mid-log phase cells were diluted to an OD600 of 0.4 ...
-
bioRxiv - Microbiology 2024Quote: ... and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX). Fragments from ∼190-250bp in length were excised ...
-
bioRxiv - Microbiology 2024Quote: ... The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher). Images were captured using an inverted fluorescence microscope (Olympus-IX73).
-
bioRxiv - Microbiology 2024Quote: ... The fluorochrome-labeled antibodies used were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... AlexaFluor 594 anti-human IgG (Thermo Fisher). The slides were mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher) ...
-
bioRxiv - Microbiology 2024Quote: ... coli strain BL21(DE3) pLysS (Invitrogen) using lysogeny broth (LB ...
-
bioRxiv - Microbiology 2024Quote: ... and 1% Tryptose phosphate browth (Trp; Life Technologies).
-
bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the PureLink Genomic DNA Mini Kit (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Amplified fragments were analyzed by capillary electrophoresis using a 3730 XL DNA Analyzer (Applied Biosystems). Chromatograms were analyzed using the Osiris software (ncbi.nlm.nih.gov/osiris) ...
-
bioRxiv - Microbiology 2024Quote: ... followed by sequencing using the S5 Ion torrent technology (Thermo Fisher), following manufacturer’s instructions ...