Labshake search
Citations for Thermo Fisher :
8451 - 8500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... Barcoded wells from the 2nd-round NGS product were pooled by experiment and loaded onto E-gel EX 2% Agarose gels (Invitrogen, G401002) for visualization ...
-
bioRxiv - Microbiology 2024Quote: ... The reaction mixture contained 10 μL platinum green hot start PCR master mix (13001014, Invitrogen, USA), 1 μL of forward primer ...
-
bioRxiv - Molecular Biology 2024Quote: ... which were then washed with 100 μL of 1X PBS pH 7.4 (Gibco, 10010023). The PBS was then removed and 50 μL of a lysis buffer added ...
-
bioRxiv - Cell Biology 2024Quote: ... The resulting products were then recombined into pDONR221 (Thermo Fisher Scientific, cat # 12536017) through a Gateway BP reaction with Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... and MkPs were purified by flow cytometry and EdU incorporation analyses were performed according to the manufacturer’s protocols (Click-iT® EdU Flow Cytometry Assay Kits, Life Technologies).
-
bioRxiv - Cell Biology 2024Quote: ... through a Gateway BP reaction with Gateway BP Clonase II Enzyme Mix (Thermo Fisher Scientific, cat # 11789020) to produce pDONR221-p24 entry clones ...
-
bioRxiv - Cell Biology 2024Quote: ... p24 sequences were transferred into modified Gateway destination vector pTSGW (UASt-Signal peptide of Tango1-GFP-Gateway cassette) (Yang et al., 2021) through Gateway LR recombination using LR Clonase II Plus enzyme (Thermo Fisher Scientific, cat # 12538120) to obtain the desired plasmids.
-
bioRxiv - Microbiology 2024Quote: ... Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes, Life Technologies), and qualification was done using the Agilent D1000TS Kit and Tapestation 4200 ...
-
bioRxiv - Microbiology 2024Quote: ... 0.5 U of DreamTaq polymerase (Fisher Scientific, Waltham, MA) and 1 μl template DNA (McManamon et al. ...
-
bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA from all experiments was reverse-transcribed to cDNA using the RevertAid cDNA prep kit (K1621, Thermo Fisher Scientific). For qPCR assays and higher abundance genes ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 40 ng of Cas7-11 expressing plasmid were cotransfected using Lipofectamine 3000 (Thermo Fisher Scientific, L3000001) according to manufacturer standards.
-
bioRxiv - Molecular Biology 2024Quote: ... sodium pyruvate and GlutaMAX (Thermo Fisher Scientific, 35050079) and supplemented with 10% (vol/vol ...
-
bioRxiv - Molecular Biology 2024Quote: ... washed once with 1X PBS (Gibco, 10010023) and replaced with 100 μL (for 24 wells) ...
-
bioRxiv - Molecular Biology 2024Quote: ... whole cell or nuclear extracts were incubated with 2.5 µg of anti-FLAG antibody bound to 50 µl Dynabeads Protein G (Invitrogen #10003D) overnight at 4°C with gentle rotation ...
-
bioRxiv - Molecular Biology 2024Quote: ... using Lipofectamine 3000 (ThermoFisher # L3000001) or X-tremeGENE HP (Roche # 6366236001) ...
-
bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted with ProLong Gold Antifade (ThermoFisher #P36930). Images were acquired on a Zeiss AxioObserver Z1 confocal microscope with a 63x oil-immersion objective lens ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein lysate concentration was determined by Pierce BCA Protein Assay Kit (Life Technologies). Head lysate protein samples (40–60 μg ...
-
bioRxiv - Microbiology 2024Quote: ... Reactions were run using Applied Biosystems MicroAmp Fast 96-well plates (0.1 ml) (Life Technologies). Plates were run on QuantStudio 3 Real-Time PCR system (Thermo Fisher) ...
-
bioRxiv - Microbiology 2024Quote: ... and run in MOPS Running Buffer (ThermoFisher, NP0001) followed by transfer to PVDF membranes using a TransBlot Turbo Transfer System at 25 V ...
-
bioRxiv - Microbiology 2024Quote: TrueCut Cas9 protein-v2 (ThermoFisher, A36499), 10 pmol per target ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were loaded on a 4-12% Bis-Tris gel (ThermoFisher, NP0321BOX) and run in MOPS Running Buffer (ThermoFisher ...
-
bioRxiv - Microbiology 2024Quote: ... SYBR™ Green PCR Master Mix (Applied Biosystems, Carlsbad, CA) and 1 μM of each oligonucleotide ...
-
bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar: BD Difco, Fisher Scientific; defibrinated sheep’s blood ...
-
bioRxiv - Microbiology 2024Quote: ... supplemented with 0.5% glycerol and 0.1% Tween-80 (Fisher Scientific) or Middlebrook 7H11 Agar (Sigma Aldrich ...
-
bioRxiv - Microbiology 2024Quote: ... 1x phosphatase inhibitor) and eluted in 1x LDS Sample Buffer (ThermoFisher, NP0007) by boiling 10 minutes at 55°C.
-
bioRxiv - Microbiology 2024Quote: ... R2A (BD Difco, Fisher Scientific), LB (BD Difco ...
-
bioRxiv - Microbiology 2024Quote: ... LB (BD Difco, Fisher Scientific), tryptic soy agar with sheep’s blood (pre-made plates ...
-
bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar: BD Difco, Fisher Scientific; defibrinated sheep’s blood: Thermo Scientific, Fisher Scientific), and M9 minimal media (BD Difco ...
-
bioRxiv - Microbiology 2024Quote: ... Lysates were pre-cleared with Dynabeads Protein G (ThermoFisher, 10004D) conjugated to Normal Mouse IgG (Table 1) ...
-
bioRxiv - Developmental Biology 2024Quote: ... with the first wash including 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole; ThermoFisher Scientific) to stain nuclei ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were maintained in tissue culture dishes (Fisher Scientific Corning Costar) coated with 0.5 mg/cm2 Vitronectin (VTN-N ...
-
bioRxiv - Developmental Biology 2024Quote: ... each cage received a separate micro-plate (60 mm x 15 mm, cat. No. FB0875713A, Fisher Scientific, USA) containing moist cotton wool as an additional source for drinking.
-
bioRxiv - Developmental Biology 2024Quote: ... and nuclei were stained with DAPI (Invitrogen). Slides were mounted using ProLong Antifade Diamond (Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2024Quote: ... coated with 0.5 mg/cm2 Vitronectin (VTN-N) (Thermo Fisher Scientific) in E8 medium (StemCell Technologies ...
-
bioRxiv - Developmental Biology 2024Quote: ... Slides were mounted using ProLong Antifade Diamond (Thermo Fisher Scientific). Secondary antibodies used were donkey Alexa Fluor 488 ...
-
bioRxiv - Developmental Biology 2024Quote: ... sections were washed 3 times with PBS 1X for 10 min and incubated with secondary antibody goat anti-rabbit Alexa Fluor 568 (Invitrogen A-11011) in blocking solution for 3 hours at RT ...
-
bioRxiv - Developmental Biology 2024Quote: ... in dPBS (Gibco) (PBST ...
-
bioRxiv - Microbiology 2024Quote: ... Protein concentrations of the resulting fractions were measured Pierce Micro BCA Protein Assay Kit (Thermo Scientific).
-
bioRxiv - Microbiology 2024Quote: ... Dorsal skins were removed and placed in Trizol (Invitrogen) and immediately homogenized in BeadBug tubes for 6 cycles of 30s at speed 5 with a 1 min delay between cycles ...
-
bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar: BD Difco, Fisher Scientific; defibrinated sheep’s blood: Thermo Scientific, Fisher Scientific), and M9 minimal media (BD Difco ...
-
bioRxiv - Microbiology 2024Quote: ... tryptic soy agar with sheep’s blood (pre-made plates: Thermo Scientific; tryptic soy agar ...
-
bioRxiv - Microbiology 2024Quote: ... and 4 μl was spotted onto LB agar or on LB agar with 1% (w/v) sodium deoxycholate (DOC, Thermo Fisher Scientific), as has been previously described (56) ...
-
bioRxiv - Microbiology 2024Quote: ... Culture supernatants were filtered through 0.2 μm Nalgene Rapid-Flow Bottle Top Filters with PES membranes (Thermo Scientific) and concentrated using 15 mL ...
-
bioRxiv - Developmental Biology 2024Quote: ... The brains were kept in 4°C cold Hank’s Balanced Salt Solution 1X (HBSS) and subsequently cut in 300 µm thick slices with a vibratome (Microtome HM 650 V, Thermo Scientific). The SCN was cut out of the brain slices with a scalpel under a microscope (MZ6 ...
-
bioRxiv - Developmental Biology 2024Quote: ... qRT-PCR was performed on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) using QuantiNova SYBR Green PCR Kit (208054 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The gonad-mesonephros complex was carefully dissected out from E11.5 Swiss mouse embryos and cultured in hanging droplets of 30 µL DMEM medium (Gibco) supplemented with 10% FBS (Bovogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... Aliquots containing 10 μg of peptide from each sample were labeled with tandem mass tag (TMT) 16-plex reagents (TMTpro, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: The peptides from each High pH elution were resolved by reversed phase chromatography on a 300 x 0.075 mm column packed with ReproSil Pur C18 AQ 1.9 mm resin (Dr Maisch, Germany) using an Ultimate 3000 RSLC nano system (Thermo Fisher Scientific). The column was heated to 50 °C using a column oven (PRSO-V1 ...
-
bioRxiv - Developmental Biology 2024Quote: Peptides were detected by tandem mass spectrometry using a Q Exactive Plus hybrid quadrupole-orbitrap mass spectrometer (Thermo Fisher Scientific). The Nanospray Flex ion source (Thermo Fisher Scientific ...