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Citations for Thermo Fisher :
8201 - 8250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... detached with trypsin (Gibco), complete culture medium was added ...
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bioRxiv - Microbiology 2024Quote: ... The Roswell Park Memorial Institute (RPMI) 1640 GlutaMAXTM medium was purchased from Gibco. The Nitric Oxide (NO ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... or anti-rabbit (dilution 1:50,000, Invitrogen, G-21234) secondary antibody ...
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bioRxiv - Microbiology 2024Quote: Cryo-electron microscopy data was collected at liquid nitrogen temperature using a Titan Krios electron microscope (Thermo Fisher Scientific), equipped with a K3 Summit direct electron detector (Gatan ...
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bioRxiv - Microbiology 2024Quote: ... 1% L-Glutamine (Gln; 200 mmol.l−1; Life Technologies) and 1% Tryptose phosphate browth (Trp ...
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bioRxiv - Microbiology 2024Quote: ... a real time PCR quantification step was performed using Ion Library TaqMan™ Quantitation Kit (Thermo Fisher). Next steps included an emulsion PCR of the pools and loading on 520 chips performed using the automated Ion Chef instrument (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... the fresly isolated primary CD4+ T-cells were cultured in 96-well plates at 300,000 cells/well in 0.1 mL RPMI medium (RPMI 1640, Life Technologies, Carlsbad, CA) supplemented with 2 mM L-glutamine (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... and used for synthesis of cDNA with Superscript III reverse transcriptase and random primers (Life Technologies). Depending on the gene to be measured ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 2 mM L-glutamine (Life Technologies), 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... 0.1% TFA and peptide content was estimated with a Pierce™ Quantitative Colorimetric Peptide Assay (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... NuPAGE LDS loading dye (ThermoFisher) was added ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from the RNA using the High-Capacity Reverse Transcriptase kit (Applied Biosystems, CA, USA). The SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then incubated at 95°C during 5 minutes and separated by SDS-PAGE using 4-12% Bis-Tris gels (Invitrogen), stained for 1 hour using BioSafe-Coomassie stain (BioRad) ...
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bioRxiv - Microbiology 2024Quote: ... or a QuantStudio 3 (Thermo Fisher Scientific, Inc., Waltham, MA, U.S.A.) with two primers and probes consisting of the forward primer (5’-TGC TCA TGG TAT CAA TCT TAT CG −3’) ...
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bioRxiv - Microbiology 2024Quote: ... BS-C-1 cells (ATCC CCL-26) were grown in Dulbecco’s Modified Eagle Medium (DMEM) + GlutaMAX-I (Gibco) supplemented with 0.1 mg/mL penicillin ...
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bioRxiv - Microbiology 2024Quote: ... Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, 0.075 × 250 mm (Thermo Fisher Scientific)) with a linear gradient of 4-27.5% solvent B (0.1% FA in 98% ACN ...
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bioRxiv - Microbiology 2024Quote: ... Samples were imaged in a CM100 transmission electron microscope at 80 keV (Thermo Fisher Scientific, Eindhoven, The Netherlands).
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bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
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bioRxiv - Cell Biology 2024Quote: ... 1× N2 (Gibco, 17502048), 1× B27 (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... 1× B27 (Gibco, 17504044), 10 µM BDNF (PeproTech ...
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bioRxiv - Microbiology 2024Quote: ... IFNβ, IFNα and IFIT1) and 15x (for reference genes: ACTIN, RPL13A, YWHAZ and UBC) with Nuclease-free water (Ambion, Life Technologies). 5 μL of the diluted cDNA was then used for qPCR ...
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bioRxiv - Microbiology 2024Quote: Cell membranes were stained with FM5-95 (1 µg/ml final concentration, Invitrogen) and DNA was stained with DAPI (5 µg/ml final concentration ...
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bioRxiv - Microbiology 2024Quote: ... and stained with secondary antibody goat anti-mouse AlexaFuor594 (Thermo Fisher Scientific), unless specified ...
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bioRxiv - Microbiology 2024Quote: ... stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer for 1h at RT and IEDDA-clicked as described above ...
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bioRxiv - Microbiology 2024Quote: ... cell membranes were stained with Nile red (200 ng/ml, Invitrogen). To reduce background caused by excess membrane dye ...
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bioRxiv - Microbiology 2024Quote: ... and 10% fetal calf serum (FCS; Gibco). During infection experiments ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was subsequently diluted 3x (for target genes: IFNβ, IFNα and IFIT1) and 15x (for reference genes: ACTIN, RPL13A, YWHAZ and UBC) with Nuclease-free water (Ambion, Life Technologies). 5 μL of the diluted cDNA was then used for qPCR ...
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bioRxiv - Microbiology 2024Quote: ... the coverslips were inverted onto a 30- µl droplet of freshly prepared CuAAC click reaction mix [10 µM N3-AlexaFluor647 (Thermo Fisher Scientific), 1 mM CuSO4 ...
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bioRxiv - Microbiology 2024Quote: ... and genomic DNA libraries were generated according to the manufacturers’ protocols using the Ion Xpress™ Plus gDNA Fragment Library Preparations (Life Technologies). Bar-coded libraries were prepared using the Ion Plus fragment library kit (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing was performed on the Ion Proton system using the Ion PI Chips (Life Technologies) according to the manufacturers’ protocols ...
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bioRxiv - Microbiology 2024Quote: ... coli SM10 were grown in LB broth (Fisher Scientific BP97235) or LB+1.5% agar supplemented when needed with carbenicillin (100 µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... and HER911 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; Gibco) supplemented with non-essential amino acids (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of isolated DNA was performed with the Qubit 4 fluorometer and the dsDNA HS Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). DNA was sent to SeqCenter (Pittsburgh ...
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bioRxiv - Cell Biology 2024Quote: Raji cells were stained with 1.0 µM Celltracker Deep Red (Invitrogen, C34565) for 30 minutes in supplement-free RPMI1640 at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... pH 7.4) and pre-charged with 25% (v/v) Fluo-4 acetoxymethyl (Invitrogen, # F14201) for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were applied with Prolong Gold Antifade reagent with DAPI (P36935, Thermo Fisher Scientific, USA) and covered with cover glass before proceeding with imaging ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed 2X in PBS and stained with 10 µg/mL Wheat-Germ Agglutinin (WGA) AlexaFluor 647 conjugate (Invitrogen, W32466) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular Ki67 staining was performed using the FoxP3 transcription factor staining kit per manufacturer protocol (ThermoFisher, 00-5523-00). Cells were fixed in 2% paraformaldehyde for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 10-30 µg of protein was loaded onto precast gel (NP0321, NuPAGE™ 4- 12%, Bis-Tris, Mini Protein Gels, ThermoFisher Scientific, USA) and resolved at 135 volts for 90 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Cultured 5637 human bladder cancer cells were lysed in Pierce IP Lysis Buffer (ThermoFisher, 87787), and incubated for 30mins at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The qRT-PCR reaction was run in QuantStudio™3 Real-Time PCR System (Applied Biosystems™, USA) using the following settings ...
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bioRxiv - Cell Biology 2024Quote: ... ATF6 (Thermo Fisher, PA5-20216), XBP1 (CST ...
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bioRxiv - Cell Biology 2024Quote: Bladders of young WT and Ifrd1−/− mice were collected and homogenized in TRIzol™ Reagent (15596026, Thermo Fisher Scientific, USA) to extract total RNA followed by DNase 1 treatment (18068-015 ...
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bioRxiv - Cell Biology 2024Quote: ... p-PERK (Thermo Fisher, PA5-102853), eIF2α (CST ...
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bioRxiv - Cell Biology 2024Quote: ... to extract total RNA followed by DNase 1 treatment (18068-015, Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Total protein was quantitated using a BCA assay (Pierce™ BCA Protein Assay Kit, 23225, Thermo Fisher Scientific, USA). 10-30 µg of protein was loaded onto precast gel (NP0321 ...
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bioRxiv - Cell Biology 2024Quote: ... One microgram of total RNA was utilized to perform cDNA synthesis using SuperScript™ II Reverse Transcriptase (18064-014, Thermo Fisher Scientific, USA) following manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Coverslips were mounted onto slides in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific), let dry over two nights and imaged in a Leica SP8 FALCON cLSM as described.
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bioRxiv - Microbiology 2024Quote: ... and the Ion Xpress DNA bar coding kit (Life Technologies) according to the 200-base-read Ion Proton libraries instructions of the manufacturer ...