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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: RNA concentration was measured using Qubit™ RNA HS Assay Kit (ThermoFisher Scientific, #Q32852). Libraries from oocyte cytoplasm RNA fractions were prepared with a starting amount of 100 ng and amplified with 15 PCR cycles ...
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bioRxiv - Cell Biology 2024Quote: ... organoids were removed from Matrigel and broken into smaller cell clusters with TrypLE Express (Gibco). Cell clumps were electroporated using Mouse/Rat Hepatocyte NucleofectorTM Kit (Lonza ...
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bioRxiv - Cell Biology 2024Quote: ... The culture medium for maturation consisted of ɑ-MEM (Gibco, USA) supplemented with 1% ITS-G (Macklin ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with cycloheximide (CHX) (Thermo Fisher Scientific, USA), harvested and lysed in Ribosome Lysis Buffer as described above ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then treated with 100 µg/ml of cycloheximide (CHX) (Thermo Fisher Scientific, USA) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Fractions of 650µl were collected and proteins were precipitated using trichloroacetic acid (TCA) (Thermo Fisher Scientific, USA) precipitation ...
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bioRxiv - Cell Biology 2024Quote: ... tetracycline induced GFP-RasV12 cells were transfected with 100ng siRNA oligos targeting Myc using Lipofectamine 3000 (Invitrogen) (siRNA siMyc1 ...
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bioRxiv - Cell Biology 2024Quote: ... Desalting was performed using C-18 tips (Thermo Fisher Scientific, USA), equilibrated with three washes in 100% CH3CN and 0.1% TFA in LC/MS grade H2O ...
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bioRxiv - Cell Biology 2024Quote: ... to block non-specific RNA binding in 0.15M KCl Wash Buffer supplemented with 0.5mM DTT and 40U/ml RiboLock RNase inhibitor (Thermo Fisher Scientific, USA). Final washes were performed three times in 0.35M KCl Wash Buffer (10mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... and DAPI (ThermoFisher Scientific). DAPI negative ...
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bioRxiv - Cell Biology 2024Quote: ... and penicillin–streptomycin solution (P4333 - Sigma-Aldrich; #15140-122- Gibco). All cells were grown at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by Alexa fluor secondary antibody incubation for 60 minutes at room temperature and Hoechst (ThermoFisher) (table 1 ...
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bioRxiv - Cell Biology 2024Quote: HeLa cells were transfected with HA-RPS27A-Myc or HARPS27AP5-Myc constructs using Lipofectamine LTX reagent (Thermo Fisher Scientific, USA), and were incubated for 48 h ...
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bioRxiv - Cell Biology 2024Quote: ... They are then transferred to the DMEM/F12 buffer (Gibco, USA) and placed in a thermostatic shaker (Leopard ...
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Complementary Cytoskeletal Feedback Loops Control Signal Transduction Excitability and Cell PolaritybioRxiv - Cell Biology 2024Quote: ... Cells were cultured in RPMI medium 1640 with L-glutamine and 25 mM HEPES (Gibco; 22400-089) supplemented with 15% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cultures were then allowed to egress for 30 min at 37 °C in media supplemented with 1 μM cytochalasin D (Invitrogen). After incubation ...
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bioRxiv - Cell Biology 2024Quote: Primary antibodies used in the IFAs included rabbit-anti-HA (SG77; ThermoFisher Scientific; 1:100), mouse-anti-RAP1 (2.29 ...
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bioRxiv - Cell Biology 2024Quote: ... and mounted using ProLong Gold Antifade Reagent (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... as determined with a TetraSpeck Fluorescent Microspheres Size Kit (Invitrogen). This compares to 0.6 and 2.9 microns for a 40x 0.95 NA air lens employed on a standard spinning disc microscope (Nikon CSU-W1 spinning disk with Hamamatsu Orca Fusion camera ...
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Complementary Cytoskeletal Feedback Loops Control Signal Transduction Excitability and Cell PolaritybioRxiv - Cell Biology 2024Quote: ... and 1% penicillin-streptomycin (Thermo Fisher Scientific; 15140122). Cells were split at a density of 0.15 million cells/ml and every 3 days ...
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bioRxiv - Cell Biology 2024Quote: ... Transfection of T-REx293 cells with siRNA was performed using Lipofectamine RNAiMAX (Thermo Fisher Scientific, Waltham, MA) according to the manufacturer’s instructions ...
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Complementary Cytoskeletal Feedback Loops Control Signal Transduction Excitability and Cell PolaritybioRxiv - Cell Biology 2024Quote: ... supplemented with 15% heat-inactivated fetal bovine serum (FBS; Thermo Fisher Scientific; 16140071) and 1% penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged at 13000 RPM at 4C and supernatant (lysate) was incubated overnight with either antibody-conjugated Dynabeeds (Life Technologies XXXX) at 4C rotating ...
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bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation was performed by adding 2 mg of P-CREB antibody to protein A/G Dynabeads (Life Technologies, USA) and incubating the mixture overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:10 in H20 and was used for quantitative PCR using Power SYBR Green PCR Master Mix (ThermoFisher #4368708) following manufacturer protocol and run on Applied Biosystem QuantStudio Real-Time PCR System using targeted primer and reference primer sets (See Primer Table).
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bioRxiv - Cell Biology 2024Quote: ... The membrane was incubated overnight at 4C with primary antibodies in TBST in 5% Bovine Serum Albumin (BSA) and probed with horseradish peroxidase-conjugated secondary antibodies and developed with SuperSignal West Pico Plus chemiluminescent substrate (ThermoFIsher #34580) and imaged using an Amersham Imager 600 ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were separated using an UltiMate3000 (Dionex) HPLC system (Thermo Fisher Scientific, San Jose, CA, USA) using a 75 μm ID fused capillary pulled in-house and packed with 2.4 μm ReproSil-Pur C18 beads to 20 cm ...
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bioRxiv - Cell Biology 2024Quote: The QExactive HF (Thermo Fisher Scientific, San Jose, CA, USA) mass spectrometer was configured following a data-independent acquisition (DIA ...
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bioRxiv - Cell Biology 2024Quote: ... 4 mM 2-Deoxy-D-glucose (2DG; ACROS Organics), 0.5 μM Thapsigargin (Tg ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems, Thermo Fisher Scientific, Catalog number A25742) in CFX384 machine (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was performed using PowerUp SYBR Green Master Mix (Applied Biosystems, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 300μl of Pierce Glutathione Agarose (Thermo Scientific, #16101) that had been pre-treated with cold PBS was introduced to the lysate supernatant ...
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bioRxiv - Cell Biology 2024Quote: Isolated RNA was reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (ThermoFisher #4368813). cDNA was diluted 1:10 in H20 and was used for quantitative PCR using Power SYBR Green PCR Master Mix (ThermoFisher #4368708 ...
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bioRxiv - Cell Biology 2024Quote: ... IMR90 and HEK293T cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 100 units ml−1 penicillin and 100 µg ml−1 streptomycin (Invitrogen). IMR90 cells were cultured under physiological oxygen (3%) ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa fluor-647 labeled secondary antibody (1:300, Invitrogen, catalog number A21244) was used to detect the primary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... Following dialysis in Slide-A-Lyzer Dialysis Cassettes (Thermo Scientific, #66380) using a solution containing 50mM Tris-HCl (pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... The QExactive HF (Thermo Fisher Scientific, San Jose, CA, USA) mass spectrometer was configured following a data-dependent acquisition (DDA ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were separated using an UltiMate3000 (Dionex) HPLC system (Thermo Fisher Scientific, San Jose, CA, USA) using a 75 μm ID fused capillary pulled in-house and packed with 2.4 μm ReproSil-Pur C18 beads to 20 cm ...
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bioRxiv - Cell Biology 2024Quote: ... IP samples were run on a 4-12% Bis-Tris gel and washed three times with water before staining with Gelcode Blue Stain Reagent (Thermo Fisher 24590) overnight rocking ...
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bioRxiv - Cell Biology 2024Quote: ... Cleared lysate was flowed over pre-equilibrated HisPur™ Ni-NTA Resin (Thermo Fisher #88223) in a gravity column ...
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bioRxiv - Cell Biology 2024Quote: ... 100 nM of Mitotracker Red CMXRos (ThermoFisher), previously established for MMP measurements (Pendergrass et al. ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of RNA was treated with DNAse I (Amplification grade) (ThermoFisher), and cDNA was synthesized using iScript™ cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 2 µg of RNA was initially heated up at 70°C with RT buffer (Thermo Scientific; Cat #EP0441), 0.5 mM dNTP and 0.05 mM Oligo dT18 for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then washed with 1× Click-iT Perm/Wash reagent and stained with 1 μg/mL FxCycle Violet (Thermo Fisher Scientific) for 30 min at room temperature ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... 4-12% NuPAGE Bis-Tris gels (Invitrogen) were loaded with lysate equal to 5-10 adult worms ...
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bioRxiv - Cell Biology 2024Quote: ... 0.75-1 µg of sgRNA/Cas9 plasmids was transfected into XC45-6S cells using Lipofectamine LTX (Thermofisher) in a 6-well plate format ...
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bioRxiv - Cell Biology 2024Quote: ... and XBP1s (229bp) were separated in a 3 % agarose gel by electrophoresis and stained with SYBR Safe nucleic acid gel stain (Invitrogen). Additionally ...
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bioRxiv - Cell Biology 2024Quote: ... qPCR was carried out with TaqMan® probe/primers (Applied Biosystems; see Table S3 for Unique assay IDs). Beta-actin (ACTB) ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... HRP (Invitrogen, 31430). Immune complexes were detected with Immobilon Forte Western HRP Substrate (Millipore ...
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bioRxiv - Cell Biology 2024Quote: 10% Fetal Bovine Serum (FBS) (Gibco; Grand Island, NY, USA).