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Citations for Thermo Fisher :
8201 - 8250 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The genomic DNA concentration was determined using the Qubit dsDNA BR Assay Kit and Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cell proliferation was measured using the Click-iT™ Plus EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit (Thermo Fisher, C10632). Cells were treated with 10 μM EdU (5-ethynyl-2’-deoxyuridine ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration of each lysate was measured using the BCA protein assay kit according to manufacturer’s directions (Thermo-Fisher Scientific, Waltham, MA). Proteins were separated on a 10% SDS-PAGE gel with equivalent amounts of protein loaded in each lane (Thermo-Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All quantification steps of gDNA and NGS libraries were done with Qubit dsDNA HS Assay Kit (Invitrogen, ThermoFisher Scientific, Carlsbad, CA, USA).
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bioRxiv - Genetics 2022Quote: ... ddPCR amplification of the cDNA was performed on 1.33 μL of each cDNA dilution combined with 1 μL of miRNA-specific 20X TaqMan MicroRNA Reagent containing probes and primers for amplification from the TaqMan MicroRNA Assay Kit (Thermo Fisher Scientific), 10 μL of 2X ddPCR Supermix for probes (no dUTP ...
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bioRxiv - Molecular Biology 2022Quote: ... The genomic DNA concentration was measured by fluorimetry (Turner Biosystems) using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher). sgRNA sequences were amplified by PCR with the NEBNext High Fidelity PCR Master mix using barcoded primers as described previously (Yau and Rana ...
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bioRxiv - Microbiology 2022Quote: Peptide concentrations were measured for fraction two of each sample using the Quantitative Fluorometric Peptide Assay kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2022Quote: ... The quality and size of the precursor was checked on agarose gel and its concentration was measured using Qubit RNA BR (Broad Range) Assay Kit with the Qubit Fluorometer (Thermo Fisher Scientific). The concentration ranged from 1500 ng/μl to 2000 ng/μl.
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined using the Thermo Scientific™ Pierce™ BCA (bicinchoninic acid) Protein Assay Kit (Thermo Scientific™ Cat# 23225). Following concentration determination ...
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bioRxiv - Microbiology 2022Quote: ... then samples were stored at -80°C until measuring Gaussia luciferase activity using the Gaussia Luciferase Flash Assay Kit (ThermoFisher Scientific, 16158) as per the manufacturer’s directions on a FLUOstar Omega microplate reader (BMG Labtech) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The quality of the RNAs used to prepare RNA-seq libraries was checked with the Qubit RNA BR Assay Kit (Thermo Fisher, USA). SMAT libraries ...
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bioRxiv - Immunology 2022Quote: ... The final antibody concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Purified antibodies were stored at -80°C prior to use.
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bioRxiv - Neuroscience 2020Quote: ... Lysates were passed through a 0.22 μm Spin-X filter column after which total protein amount was determine using the Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Equal protein amounts were denatured in SB and subjected to SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations in the tissue lysates were measured with the aid of a bicinchoninic acid protein assay kit (Thermo Scientific, Rockford, IL). TNF-α ...
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bioRxiv - Genomics 2019Quote: ... Libraries were quantified using a Qubit® 2.0 Fluorometer and the Qubit® dsDNA BR Assay kit (Invitrogen™, Thermo Fisher, Massachusetts) and profiled using a 2100 BioAnalyzer system with the DNA High Sensitivity kit (Agilent Technologies ...
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bioRxiv - Genomics 2019Quote: ... Libraries were quantified using a Qubit® 2.0 Fluorometer and the Qubit® dsDNA BR Assay kit (Invitrogen™, Thermo Fisher, Massachusetts) and profiled using a 2100 BioAnalyzer system with the DNA High Sensitivity kit (Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... Total protein quantification was performed in samples using Pierce™ BCA (Bicinchoninic Acid) Protein Assay Kit (Thermo Fisher Scientific Inc. Waltham, MA) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: Luciferase activity assay in the supernatants of transfected C6/36 cells with chimeras and wild type constructions were determined with Pierce™ Gaussia Luciferase Glow Assay Kit (Thermo Scientific). Percentage of luciferase activity was normalized with DMSO treatment.
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bioRxiv - Immunology 2020Quote: ... sonicated 3 three times for 10 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 µg/µl of cell lysate proteins per lane were resolved on SDS-PAGE (10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Immunology 2020Quote: ... Library concentration was assessed with a BioTek Gen5 Wellplate reader with the Quant-iT PicoGreen dsDNA Assay kit (Thermo Fisher; CAT # P11496), and analyzed on the Bioanalyzer to ensure appropriate size distributions and rule out adaptor contamination.
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bioRxiv - Neuroscience 2021Quote: ... The library quality was checked using an Agilent 2100 Bioanalyzer and concentration was measured by a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and adjusted to 2nM before sequencing (single end ...
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bioRxiv - Plant Biology 2021Quote: Total proteins were extracted from 100 mg of foliar tissues with the IP buffer and quantified with a Pierce BCA protein assay kit (Thermo Fisher Scientific). Afterward ...
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bioRxiv - Neuroscience 2021Quote: ... the cleared supernatant was collected and the protein content in the soluble fraction was determined using the Pierce® BCA Protein Assay Kit (Thermo Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The barcode sequencing libraries were quantified using a Qubit 2.0 with a Qubit™ dsDNA HS Assay Kit (Invitrogen P/N Q32854) and the quality of the libraries were performed on a 2100 Bioanalyzer from Agilent using an Agilent High Sensitivity DNA kit (Agilent P/N 5067-4626) ...
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bioRxiv - Microbiology 2020Quote: ... The DNA concentration was assessed using the Qubit ds DNA HS Assay Kit in a Qubit Fluorometer 3.0 (Thermo Fisher Scientific, USA). Whole genome sequencing of clones was performed via 2×250 bp paired end sequencing (Nextera XT sample ...
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bioRxiv - Physiology 2021Quote: The extent of the apoptotic damage was monitored using a deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay kit (Thermo Scientific; C10618). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... samples were quantified with Qubit 1X dsDNA HS Assay Kit using an Invitrogen Qubit 4.0 Fluorometer (Thermo Fisher Scientific, Foster City, CA) and homogeneously pooled (1 pmol/ pool of PCR products ...
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bioRxiv - Microbiology 2020Quote: ... The RNA transcripts were purified with Ampure XP beads (Belckman Counter, USA) and quantified with a Qubit fluorometer by using a Qubit RNA HS Assay Kit (Thermo Fisher Scientific). All commercial reagents were used following manufacturer instructions.
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bioRxiv - Microbiology 2021Quote: ... aliquots of SAGs were transferred to replica plates for DNA yield quantification using a Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific). For sequencing analysis ...
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bioRxiv - Microbiology 2021Quote: ... Wells were pooled based on the measured concentration using the Eppendorf epMotion 5075vtc robot and the final pool quantified using the Qubit Broad Range Assay Kit (Thermo Fisher Q32853). Pools were run on a gel to confirm clean DNA before sequencing with MiSeq 2×250 V2 chemistry.
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bioRxiv - Cancer Biology 2021Quote: ... at 0.6X SPRI (solid-phase reversible immobilization) followed by a 1X SPRI and quantified using a Qubit dsDNA High Sensitivity assay kit (Thermo Fisher Q32854). Library preparation was performed using Nextera XT DNA tagmentation (Illumina FC-131-1096 ...
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bioRxiv - Microbiology 2021Quote: ... and DNA quantity for long read sequencing was measured on a Qubit™ 2.0 Fluorimeter using the dsDNA BR Assay Kit (ThermoFisher Scientific, USA). DNA quantity for short read sequencing was measured using Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: Translation activity in oocytes was detected by the Click-iT® HPG Alexa Fluor® 594 Protein Synthesis Assay Kit (ThermoFisher C10429). Oocytes were cultured in M2 medium containing milrinone (2.5 μM ...
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bioRxiv - Developmental Biology 2022Quote: The quantity and quality of the extracted RNA was assessed using a Thermo Fisher Scientific fluorometer (Qubit 4.0) with the Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Q10211) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer RNA Kit (Agilent ...
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bioRxiv - Microbiology 2022Quote: The level of hydrogen peroxide formation under antibiotic treatment was measured with the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Invitrogen, ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: RNA-seq libraries were quantified using a Qubit® fluorometer and Qubit® dsDNA HS Assay Kit (Invitrogen™/Thermo Fisher Scientific), while library quality checks were performed using an Agilent 2100 Bioanalyzer and High Sensitivity DNA Kit (Agilent Technologies Ltd.) ...
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bioRxiv - Immunology 2022Quote: ... The final Spike protein concentration was determined by measuring the absorbance at 562 nm using Pierce™ BCA Protein Assay Kit (Thermo Scientific). Final proteins were dispensed in aliquots of 0.5 ml each and stored at -80°C prior to use.
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bioRxiv - Bioengineering 2022Quote: The cell proliferation was analyzed by quantifying the total DNA content of samples using the Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher). Lamda DNA standard was used for generating the calibration curve ...
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bioRxiv - Immunology 2022Quote: ... The resulting cDNA was evaluated for quantity and quality using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: ... or CellEvent Senescence Green Flow Cytometry Assay Kit was used to detect cellular senescence via β-galactosidase hydrolysis (ThermoFisher, Cat. No. C10840) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: Cell proliferation was analyzed by quantifying the total DNA content of samples using Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher). Lambda DNA standard was used for generating a calibration curve ...
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bioRxiv - Pathology 2019Quote: ... Total nucleic acids from spiked or unspiked specimens were extracted and analyzed by qPCR assays for various pathogens as described previously(29) and DNA concentration was measured in a Qubit 2.0 fluorometer using the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, Inc.). To serve as an internal control for extraction ...
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bioRxiv - Microbiology 2019Quote: ... Protein levels were measured in triplicate for each sample using 10 µl from the pellet treatment and the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Intracellular concentrations measured by mass spectrometry were then normalized to protein levels.
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bioRxiv - Genomics 2019Quote: ... The library fragment size was assessed using the Agilent TapeStation and library concentration was determined using Qubit dsDNA HS Assay Kit (Thermo Scientific, USA). The library was then sequenced using the Illumina MiSeq platform with a paired-end 300 bp MiSeq reagent kit v3 (Illumina Inc ...
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bioRxiv - Zoology 2020Quote: ... The RNA concentration was measured using the Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies ...
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bioRxiv - Cancer Biology 2019Quote: The protein concentration of each sample was measured by using a BCA assay using the micro bicinchoninic acid (BCA) kit (Thermofisher scientific, India) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Protein pellets were dissolved in 200 uL 5% SDS solution and protein concentrations were determined using Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific).
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bioRxiv - Immunology 2019Quote: ... NETs were quantified in the culture supernatant via the Quant-iT™ PicoGreen® dsDNA Assay kit (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... EdU staining was performed following manufactures instructions (Click-iT™ Plus EdU Alexa Fluor™ 350 Flow Cytometry Assay Kit, C10645, Invitrogen), followed by intracellular staining in 1x saponin (Click-iT™ Plus EdU Alexa Fluor™ 350 Flow Cytometry Assay Kit ...
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bioRxiv - Cell Biology 2019Quote: ... followed by intracellular staining in 1x saponin (Click-iT™ Plus EdU Alexa Fluor™ 350 Flow Cytometry Assay Kit, C10645, Invitrogen).