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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... the TaqMan Scorecard Assay (Applied Biosystems) was used according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... and Qubit dsDNA HS Assay (ThermoFisher), respectively ...
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bioRxiv - Bioengineering 2024Quote: Quant-iT PicoGreen dsDNA Assay (ThermoFisher) was employed to quantify cell numbers (N = 4 ...
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bioRxiv - Neuroscience 2024Quote: ... AKAP12 (assay ID: s18435, ThermoFisher Scientific), MAFF (assay ID ...
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bioRxiv - Microbiology 2024Quote: ... High Sensitivity Assay (Thermo Fisher Scientific). The concentration values were calculated using the Thermo Fisher DNA Copy Number and Dilution Calculator with default settings for molar mass per base pair ...
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bioRxiv - Cancer Biology 2021Quote: The concentration of H2O2 in APAM was measured using the Amplex Red Hydrogen Peroxide/Peroxidase Assay Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocols as previously described (17) ...
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bioRxiv - Cancer Biology 2021Quote: Cell lysates were extracted using Triton X-100 lysing buffer and lysate was quantified using Pierce BCA Protein Assay Kit (ThermoFisher Scientific – 23225). 20 μg of protein for each cell lysate was run on a 4-15% SDS gel (Bio-Rad – 4561083DC ...
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bioRxiv - Microbiology 2020Quote: ... the RNA preparation samples were tested by performing standard spectroscopic measurements (Nanodrop One) and using the Qubit 1X dsDNA HS assay kit (Thermo Fisher Scientific). RNA was quantified using the Qubit RNA HS assay kit.
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bioRxiv - Cell Biology 2020Quote: ... The resultant mixture was centrifuged at 14,000 g and the supernatant was used to estimate the total protein by using Pierce™ BCA Protein Assay Kit (Thermo scientific). Equal quantity of protein (300 μg ...
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bioRxiv - Cell Biology 2020Quote: A total of 50 μg of protein from whole-cell or isolated mitochondria were estimated using a Pierce BCA Assay Kit (Thermo Fisher Scientific). Pellets were solubilised in solubilisation buffer (1 % [w/v] SDC ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
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bioRxiv - Cell Biology 2020Quote: ... Membrane pellets were gently resuspended in 100 μl SEM and protein content was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) to normalize total protein concentration across samples ...
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bioRxiv - Cell Biology 2020Quote: ... The protein content of preovulatory follicles from eCG-injected mice is ~6 μg (Pierce BCA protein assay kit, 23225, Thermo Fisher Scientific). The protein content of follicles (~320-400 μm diameter ...
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bioRxiv - Cell Biology 2020Quote: S phase status was monitored by measuring 5-ethynyl-2’-deoxyuridine (EdU) incorporation with the Click-iT™ Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (ThermoFisher, C10632) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... TAG amounts were normalized to the total protein amounts in each sample using a Pierce TM BCA Protein Assay Kit (Thermo Fisher Scientific). The data were analyzed using a Student’s t test.
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bioRxiv - Genetics 2021Quote: ... The gDNA sample integrity was controlled on 1% agarose gel and quantified on Nanodrop and Qubit using the Qubit™ dsDNA BR Assay Kit (Invitrogen™). 2 × 2 µg of gDNA were digested in parallel with 50 units of DpnII (NEB #R0543L ...
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bioRxiv - Microbiology 2020Quote: The proteins from cell lysates and isolated mitochondria were analysed by separating 10-40 μg of protein (concentrations determined using Pierce BCA Protein Assay kit, Thermo Fisher Scientific), by SDS-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of the MDA amplified and purified DNA was measured by Qubit dsDNA HS Assay Kit (ThermoFisher Scientific, Waltham, MA, USA). Random shotgun libraries were constructed using the Nextera XT kit (Illumina ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The quantity and purity of all RNA samples was checked using a nanodrop spectrophotometer and a Qubit™ RNA HS assay kit (Invitrogen™).
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bioRxiv - Evolutionary Biology 2020Quote: ... purity and integrity of all RNA samples was checked using a nanodrop spectrophotometer and Qubit™ RNA HS assay kit (Invitrogen™), as well an Experion™ bioanalyser with a prokaryotic RNA standard sensitivity analysis kit (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... The quantity and quality of the extracted RNA was assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit RNA BR Assay Kit (Thermo Fisher Scientific, Q10211) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer RNA Kit (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... The quantity and quality of the generated NGS libraries were evaluated using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...
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bioRxiv - Developmental Biology 2021Quote: ... cell proliferation assays were carried out using Click-It EdU Cell Proliferation Kit for Imaging Alexa Flour™ 647 (Thermo Fisher Scientific). Larvae were treated with EdU at a final concentration of 10 μM in FSW and let to grow for 2 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were treated with 10 µM EdU using a Click-iT EdU Alexa Fluor 488 flow Cytometry Assay Kit (Thermo Fisher, C10425) and processed as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: We quantified the obtained DNA using the Quant-iT™ dsDNA BR Assay Kit (product nr. Q33130; Thermo Fisher Scientific, Waltham, USA) in the Spark® 10M multimode microplate reader (Tecan Group Ltd ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA concentrations were estimated using a fluorimeter and fluorescent DNA-binding dye (Qubit™ dsDNA BR Assay Kit; Thermo Fisher Scientific, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were stored at - 80°C and protein concentrations were further determined using the Pierce™ BCA Protein Assay Kit (23225, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... The concentration of QuantSeq libraries mRNA-seq library was quantified using the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific cat. no. Q32851) on a Qubit 2.0 Fluorometer ...
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bioRxiv - Molecular Biology 2021Quote: ... Removal of residual DNA was confirmed by DNA-specific quantification using a Quant-IT dsDNA Broad Range Assay Kit (Thermo Fisher Scientific). For cDNA synthesis ...
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bioRxiv - Neuroscience 2021Quote: ... The flow-through for each sample was collected and protein was quantified using the Pierce detergent compatible Bradford assay kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: The concentration of RNA in the lysates were measured using the Quant-iT RNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and Infinite M1000 PRO (TECAN ...
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bioRxiv - Molecular Biology 2021Quote: ... the P2 fraction was brought up in 400uL of PBS and protein concentrations were measured using the QuBit Protein Assay Kit (Thermo Fisher, Q33211). Samples were precleared with 15mg of DEPC treated (to inactivate RNAse ...
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bioRxiv - Immunology 2022Quote: ... The quantity of amplicons was measured with the Qubit 4.0 fluorometer using the 1X dsDNA HS Assay Kit (Thermo Fisher Scientific, USA) and the sequencing libraries were prepared using the Nextera DNA Flex Prep kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... The DNA was stored at 4°C overnight to facilitate DNA homogenization and then quantified using a Qubit dsDNA HS Assay Kit (Molecular Probes/Life Technologies). The labeled DNA was stained with an intercalating dye and left to stand at room temperature for at least 2 hrs before loading onto a Bionano Chip.
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bioRxiv - Microbiology 2021Quote: ... The protein concentration in the cell free extracts was determined with a BCA assay kit (Pierce, Thermo Scientific, Rockford, IL, United States). Enzyme assays were prepared as follows ...
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bioRxiv - Genomics 2020Quote: ... RNA specific fluorescent dye is used to quantify extracted RNA using the Qubit RNA XR assay kit and the Qubit Flourometer system (ThermoFisher, MA, USA). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... permeabilised in ice-cold 100% methanol for 10 minutes at -20°C and labelled using an Alexa Fluor™ 647 HCS assay kit (ThermoFisher Scientific). Cells were blocked in 5% normal goat serum and 2% BSA for 1 hour ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were pulse labeled for 2 hours with 10 µM EdU and stained using the Click-iT EdU Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... The signal was normalized to the amount of protein in the sample as determined by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to manufacturer instructions.
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bioRxiv - Physiology 2019Quote: ... Data was normalized for the amount of adipose tissue macrophages by determining the concentration of DNA per well (Quant-iT dsDNA Assay Kit high sensitivity, Thermo Fisher Scientific) and normalized for gWAT explants to the weight per explant.
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 10,000 x g for 30 min at 4 °C before supernatants were collected and protein content was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Colorimetric protein assays were conducted using commercial human IL-8 ELISA and mouse IL-6 ELISA kits (Invitrogen; 88-8086, 88-7064) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: The DNA from the above step was quantified using a Qubit DNA high sensitivity assay kit and Qubit 2.0 machine (Thermo Fisher Scientific, UK). The DNA concentration in each well was normalised to the lowest concentration sample ...
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bioRxiv - Developmental Biology 2020Quote: ... gRNAs were visualized in a 2% agarose stained by ethidium bromide to check for RNA integrity and quantify using Qubit™ RNA BR Assay Kit (ThermoFisher, Q10210). gRNAs targeting tbxta and tyr CDS in zebrafish and rx3 in medaka were in vitro transcribed from PCR containing a pool of different gRNA primers at equal concentration ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We quantified the protein content in the clarified lysates of the damselflies using Pierce™ BCA protein assay kit (Thermo Fisher Scientific). We used the Bovine Serum Albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was isolated using RNA-Solv® Reagent and reverse-transcribed with TaqMan MicroRNA Reverse Transcription Kit and TaqMan MicroRNA Assays for each miRNA (Applied Biosystems). Real-time PCR was performed in a total volume of 20 µL on a Step One Plus real-time PCR system (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2019Quote: ... The newly generated RNA transcripts were separated from the streptavidin magnetic beads and their quantity measured using the Qubit RNA HS Assay Kit (Thermo Fisher, Q32852).
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bioRxiv - Physiology 2020Quote: ... Protein pellets from each aliquot per sample were pooled and the total protein content were measured using BCA protein Assay kit (ThermoFisher Scientific, Australia). The protein disulphide bonds were reduced with 10 mM dithiothreitol for 30 min at 60 °C and the cysteine residues were alkylated with 20 mM iodoacetamide for 30 min at 37 °C in the dark ...
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bioRxiv - Cell Biology 2019Quote: ... aliquots of cell suspensions were used to check for equal cell numbers by fluorescent staining (CyQuant Cell Proliferation Assay Kit; Molecular Probes, #C7026). In the setting 2 ...
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bioRxiv - Biochemistry 2019Quote: ... Taqman MicroRNA Reverse Transcription Kit and Taqman Universal Master Mix II with the TaqMan MicroRNA Assay (Applied Biosystems, Foster City, CA, USA) were to test miR-216a-5p expression ...