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Citations for Thermo Fisher :
8351 - 8400 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: RNA-seq was performed at the DNA Core at the CCHMC facility with 10 ng – 150 ng of total RNA used after quantification by Qubit RNA HS assay kit (Cat #Q32852; Invitrogen, Waltham, MA). Based RNA integrity value above 7 determined by the spectrofluorometric measurement RNA samples was poly-A selected and reverse transcribed using Illumina’s TruSeq stranded mRNA library preparation kit (Cat# 20020595 ...
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bioRxiv - Microbiology 2024Quote: ... RNA concentrations were quantified using the Qubit 2.0 Fluorometer and the RNA High Sensitivity Assay Kit (RNA HS, Thermo Fisher, Berlin, Germany).
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bioRxiv - Microbiology 2024Quote: ... the concentration and integrity (RIN) of isolated RNA were determined using the Quant-iT™ RiboGreen™ RNA Assay Kit (Thermo Fisher) and an RNA Standard Sensitivity Kit (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... the DNA samples obtained were quantified using the Qubit dsDNA BR Assay kit and Qubit 4.0 fluorometer (Life Technologies Corporation, Eugene, OR). As described by Bari et al ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified proteins was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, United States) as described previously (Choi et al. ...
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bioRxiv - Microbiology 2024Quote: ... Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA, USA) before being used for in vitro acetylation.
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bioRxiv - Molecular Biology 2024Quote: ... after which then genomic DNA was isolated for detection of gene editing efficiency using the GeneArt genomic cleavage assay kit (Invitrogen, catalog #A24372). Hep3Bcas9 cells had high cleavage efficiency and thus was chosen for generating knockout cell lines using CRISPR ...
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bioRxiv - Immunology 2024Quote: ... The concentration of each sample was determined using a Qubit fluorometer and the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32851). To prepare the final libraries ...
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bioRxiv - Developmental Biology 2024Quote: ... Generated cDNA and both types of libraries were evaluated for quantity and quality using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32851) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA was quantified by a Qubit® DNA Assay Kit in Qubit® 2.0 Fluorometer (Catalog No. Q32866, Thermo Fisher Scientific). A total amount of 0.6 µg genomic DNA per sample was used as input material for the DNA sample preparation ...
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bioRxiv - Cell Biology 2024Quote: ... and processed to generate cDNA using 10X Genomics v2 chemistry according to the Chromium Single Cell 3’ Reagents kits v2 user guide.32 After undergoing quantification and quality control of cDNA libraries using the Qubit dsDNA HS Assay kit (Life Technologies Q32851), high-sensitivity DNA chips (Agilent 5067-4626) ...
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bioRxiv - Cell Biology 2023Quote: ... was added as a supplement to the lysis buffer and the protein concentration was measured using a Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... RIN) of the extract were assessed by a fluorometric measurement (Quant-iT RNA Assay Kit, Broad Range from ThermoFisher Scientific, Massachusetts, USA) on a Spark reader (Tecan ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA quality and concentration were determined by MOPS-formaldehyde denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: Protein synthesis was assessed using the Click-iT® L-homopropargylglycine (HPG) Alexa Fluor 488 Protein Synthesis Assay Kit (Molecular Probes, USA), which measures newly synthesized proteins through quantification of the fluorescent HPG signal ...
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bioRxiv - Immunology 2024Quote: DENV-2 and ZIKV viral RNA for real-time PCR assay was extracted from 200uL of virus isolate (previously tittered by focus reduction neutralization assay) and from acute serum samples using a MagMax Viral/Pathogen Nucleic Acid Isolation Kit (Applied Biosystems, MA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Extracted DNA was quantified using a dsDNA broad-range assay kit (Catalogue number: Q32850) on the Qubit 3 fluorimeter (ThermoFisher Scientific UK) following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... DNA concentration and DNA fragment length were assessed using the Qubit dsDNA BR Assay kit on the Qubit Fluorometer (Thermo Fisher Scientific) and the Genomic DNA Screen Tape on the Agilent 4150 TapeStation system (Agilent Technologies) ...
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bioRxiv - Immunology 2024Quote: ... To measure the protein content of the lysate a Qubit 4 fluorometer was used using the Qubit protein assay kit (Thermo Fisher Scientific). Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: Lung homogenates were prepared as described above for TCID50 assays and cytokines were measured using a ProcartaPlex Luminex kit (Thermo Fisher Scientific) on a MAGPIX Instrument (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... All samples were quantified and normalized using a protein assay kit known as bicinchoninic acid (Thermo Fisher Scientific, Waltham, MA, United States). Following a 10-minute heat treatment at 95 °C ...
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bioRxiv - Bioengineering 2024Quote: ... All the OCR and ECAR values were normalized by DNA content measured for each well using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Dublin, Ireland) as previously described.[167]
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bioRxiv - Cancer Biology 2024Quote: Protein translation was detected with the “Click-iT™ Plus OPP Alexa Fluor™ 488 Proteinsynthese-Assay-Kit” (Thermo Fisher Scientific, C10456) according to manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The DCF quantification was normalized on the corresponding protein amount that was quantified using the BCAprotein assay kit (ThermoFisher Scientific, Stockholm, Sweden).
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each lysate was measured using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). The 2′-3′-cGAMP content were measured by 2′-3′-cGAMP ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of each sample was determined by the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, CA, USA). Equal amounts of proteins (up to 20 μg ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C) and the protein content of the supernatant was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Reduction ...
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bioRxiv - Biophysics 2023Quote: ... The final concentration of the solution was confirmed to be 125 µM using the Pierce™ BCA Protein Assay Kit (23227, Thermo Fisher).
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified as above for the second PCR and final amplicon concentration measured using a Qubit Flex Fluorometer and Qubit dsDNA HS assay kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... We extracted and purified genomic DNA using the Qiagen DNA mini kit and quantified the amount of DNA with Quant-iT™ PicoGreen® Assay (Invitrogen). For samples with less than 50 ng DNA obtained ...
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bioRxiv - Cell Biology 2023Quote: ... and then collected for normalization of oxygen consumption to DNA content measured using a Quant-iT™ PicoGreen™ dsDNA Assay Kits (Invitrogen).
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bioRxiv - Microbiology 2022Quote: In vitro transcription of ssr7036 (consisting of the complete 5’ UTR and the first 100 nt of coding sequence) and asRNA1 substrates for RNase E assays was performed with the MEGAshortscript T7 transcription kit (Thermo Fisher Scientific). Suitable templates were PCR-amplified (primers P41 to P44 ...
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bioRxiv - Genetics 2023Quote: ... The barcoded short-read libraries were measured using a Qubit 2.0 with a Qubit dsDNA HS assay kit (Invitrogen, catalog no. Q32854) and the quality of the libraries was assessed on a Fragment analyzer (Agilent ...
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bioRxiv - Genomics 2023Quote: ... The DNA integrity of the pool was controlled on 1% agarose gel and quantified on Nanodrop and Qubit using the Qubit™ dsDNA HS Assay Kit (Invitrogen™).
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bioRxiv - Microbiology 2023Quote: ... was added to cultures to label new peptide synthesis before harvesting (Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit, Invitrogen)88 ...
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bioRxiv - Immunology 2023Quote: ... The resulting cDNA was evaluated for quantity and quality using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32851) and an Advanced Analytical Fragment Analyzer System using a Fragment Analyzer NGS Fragment Kit (Agilent ...
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bioRxiv - Immunology 2023Quote: ... they are displayed as area ratio and were normalized to protein content measured using Pierce TM BCA protein assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... DNA quantity was checked with fluorometry on the DS-11 FX instrument (DeNovix) using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and sizes were examined on a 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... the quantification of double-stranded DNA (dsDNA) was measured using a Quant-iT™ PicoGreen™ dsDNA assay kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled with 10 μM EdU for 30 min before harvesting and processed using the Click-iT® EdU flow cytometry assay kit (C10643) (Thermo Fisher). In brief ...
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bioRxiv - Microbiology 2023Quote: ... Twenty-five microliter of the slurry was centrifuged and the supernatant was subjected to protein concentration determination using Pierce TM BCA protein assay Kit (Thermo Fisher Scientific). The protein slurry was diluted to 1 ng/µl with 100 mM Tris pH 8 ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was harvested from cells according to manufacturer standards using the Quant-iT™ PicoGreen™ dsDNA Assay Kits (Thermofisher, Catalog # P11496). OCY454 cells were collected directly from scaffold or well plate using 1 mL of TE lysis buffer (10 mM Tris ...
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bioRxiv - Plant Biology 2023Quote: The RNA was measured with Qubit® RNA HS Assay Kits again and libraries were prepared with NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads (Invitrogen Life Technologies (Ambion Applied Biosystem) ...
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bioRxiv - Genetics 2023Quote: ... using respectively the salting out method or magnetic beads and were quantified using fluorimetry (Quant-iT DNA Assay kit, Broad Range, Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... The amount of affibody conjugated to the polymer was quantified by dissolving 2-3 mg of HA-Nor-Ox-Affibody in PBS at 2% w/v and determining the protein concentration of the solution using a Pierce 660 Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... fixed with 1% formaldehyde for 10 min and stained using the Click-IT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The membrane pellet was resuspended in the same buffer and the protein concentration was determined spectroscopically with the BCA protein assay kit (Thermo Fisher Scientific). The membrane fraction was supplemented with n-dodecyl-β-D-maltoside (DDM ...
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bioRxiv - Molecular Biology 2023Quote: The concentration of the samples was determined using the Quant-iT™ dsDNA Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA; Q33120) and the NanoDrop™ 3300 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentration of extracts was measured with the PicoGreen fluorophore (Quant-iT™ PicoGreen™ dsDNA Assay Kits, Thermo Fisher Scientific, USA). Bulk soil samples ...