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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Treated cells were lysed with CLB1 buffer (Zeptosens, Bayer) and quantified using a Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific 23227). Samples were diluted (100% ...
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bioRxiv - Cell Biology 2019Quote: ... cells were seeded into multi-well plates and pulse labelled with 10μM EdU for 45 minutes (Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit, Thermo Fisher Scientific, C10424). At hourly intervals for 24-32 hours a single well was harvested using TrypLE cell dissociation enzyme (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA quantity measurement of isolated DNA samples took place using a Qubit fluorometer with double stranded DNA (dsDNA) High Sensitivity Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2019Quote: Proteins were collected 48 hours post-transfection and their concentrations were measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Western blots were performed as described previously (Lee et al ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilization and Click-IT reaction were performed using the Click-iT Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (Fisher Scientific, C10458), according to the manufactureŕs protocol ...
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bioRxiv - Genomics 2019Quote: ... A combined library of equal amounts of DNA from each isolate was created based on quantifying DNA concentration using Quant-iT dsDNA Assay Kit (Thermo Fisher Scientific). As Quant-iT quantifies total DNA ...
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bioRxiv - Microbiology 2019Quote: ... Each library was quantified using the Agilent Tapestation and the Qubit™ DNA BR assay kit for Qubit 3.0® Fluorometer (Life technologies). The indexed libraries were pooled and diluted to 1.5pM for paired end sequencing (2× 81 cycles ...
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bioRxiv - Genetics 2020Quote: Total peroxidase activity (POX) was measured with the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit according to manufacturer’s instructions (Invitrogen, Carlsbad, CA). Pulverized leaves were homogenized in 20mM sodium phosphate buffer (pH 6.5 ...
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bioRxiv - Genomics 2021Quote: ... Elution was performed in nuclease-free water and 1 μL was used for measuring the concentration with the Qubit™ dsDNA High Sensitivity Assay Kit (ThermoFisher Scientific), while the size was assessed with a High Sensitivity DNA chip on an 2100 Bioanalyzer.
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bioRxiv - Genetics 2020Quote: ... The concentrations of DNA from blood and saliva samples were determined using the fluorescence-based Quant iT dsDNA Board-Range assay kit (cat # Q-33130, Life Technologies, CA). Fluorescence was measured with a Tecan Infinite M 200 Pro multimode microplate reader (Tecan ...
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bioRxiv - Developmental Biology 2020Quote: ... 1% Sodium Citrate pH6.0 (30 minutes at room temperature): Proteinase K (1/25 of stock solution from the Click-iT™ Plus TUNEL assay kit - Molecular Probes #C10617 for 30 minutes at room temperature) ...
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bioRxiv - Developmental Biology 2020Quote: ... These cells were fixed with 1% PFA overnight and OPP signal was detected using Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit (Invitrogen) as the manufacturer′s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and assessed the quality and quantity using Tecan Infinite® 200 NanoQuant and Quant-iT PicoGreen® dsDNA assay kit (ThermoFisher Scientific). We determined the sex of fish following Peichel et al ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Quality control of the RNA was performed on agarose gels and the Qubit RNA HS or BR Assay kit (Invitrogen, Carlsbad, CA) for quantification ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein extracts were diluted in PBS and quantified in duplicate using a Micro BCA Protein Assay Kit (ThermoFisher Scientific, cat no: 23235). Bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: Annexin V apoptosis and dead cell detection assay was performed with the Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit (Invitrogen, cat# V13241) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The concentration of the final pool was measured with the Qubit™ dsDNA HS Assay kit (Thermo Fisher Scientific, Waltham, MA, USA). The final pool was processed to be sequenced on the Miseq and NextSeq platforms (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted using a modified phenol-chloroform method (11, 75) and quantified with the dsDNA High Sensitivity Assay kit (Thermo Fisher Scientific) on a Qubit 3.0 Fluorometer ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% SDS and protease and phosphatase inhibitors) and stored at −20°C before sonication and quantification by a Pierce BCA Protein Assay Kit (Thermofisher Scientific, 23225). Protein lysates were heated for 5 min at 95°C in loading buffer (1% SDS ...
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bioRxiv - Bioengineering 2020Quote: ... Intensity quantification was performed using ImageJ software and the obtained values were corrected by culture media background (without having been in contact with cells) and total protein content measured using the Pierce™ BCA Protein Assay Kit (Thermo Scientific). Values were further normalized by internal negative and positive controls from the antibody assay ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined following centrifugation of the lysate at 16,000 x g at 4 °C for 20 minutes using the using a Pierce™ Coomassie (Bradford) Protein Assay Kit (23200, Thermo Scientific) with bovine serum albumin as the standard.
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bioRxiv - Cell Biology 2021Quote: ... Lysates were centrifuged at 14000 rpm for 15 minutes at 4°C and protein concentration determined with a Pierce™ BCA Protein Assay Kit (Life Technologies). High capacity neutravidin beads (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Living cell numbers and metabolic activity were measured with an MTT assay or labeled for 1 h using the LIVE/DEAD® kit (ThermoFisher Scientific) containing 2 μM calcein and 4 μM EthD-1 (Ethidium homodimer-1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear membrane and debris were pelleted by centrifugation and protein concentration of the cleared lysate was determined with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). 2μg of the anti-BAF155 antibody (ab172638 ...
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bioRxiv - Cancer Biology 2020Quote: The relative release of glutamate from cells into the extracellular medium was detected using an Amplex Red glutamate release assay kit (Thermo Fisher Scientific). Glutamate release was first normalized to the total cell number determined with the CCK8 kit at the end of the experiment ...
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bioRxiv - Cancer Biology 2019Quote: ... Levels of senescence in K562 clonal cell lines were measured using the CellEvent Senescence Green flow cytometry assay kit according to the instructions of the manufacturer (Thermo Fisher Scientific) except that cells were stained with fixable viability dye eFluor 450 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... The cell debris was removed by centrifugation and the protein concentration of the supernatants were determined using Pierce™ bicinchoninic acid (BCA) protein assay kit (Thermo Scientific) according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The 300 μl collected was aliquoted into an individual well in a 96-well microplate containing 100 μl each (3 wells total for a single well in the 24-well plate) and subjected to a CyQuant© Direct Proliferation Assay Kit (Thermo Fisher) per the manufacturers protocol ...
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bioRxiv - Microbiology 2021Quote: ... Concentrations of the amplified gene fragments were determined by a Quant-iT™ PicoGreen™ dsDNA Assay Kit (Invitrogen, Waltham, MA, USA) and used for standard curve ...
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bioRxiv - Microbiology 2021Quote: ... whereas the DNA-removing efficacy was evaluated using the Qubit 1× dsDNA HS assay kit (Thermo Fisher Scientific, Waltham, MA, United States).
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with 10 µM EdU for 3 h and analyzed using Click-iTTM EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific). Cells were recorded with a BDTM LSRII (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... Libraries were quantified using the IS7 and IS8 primers65 in a quantification assay using a DyNAmo SYBP Green qPCR Kit (Thermo Fisher Scientific) on the LightCycler 480 (Roche) ...
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bioRxiv - Microbiology 2021Quote: The release of Pi by pyrophosphatase activity of VP1 proteins in isolated yeast vesicles was measured using the PiPer ™ Phosphatase Assay Kit (P22061, ThermoFisher Scientific), according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... followed by collection of supernatants for quantification of total protein concentration using Microplate BCA Protein Assay Kit (Pierce™, Thermo Fisher Scientific). Equal amounts of protein were separated by SDS-PAGE using 4-15% SDS polyacrylamide gels (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: The enumeration of apoptotic cells was performed using the CellEventTM Caspase-3/7 Green Flow Cytometry Assay Kit (catalogue #: C10427; Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... The quantity of the sequencing-ready library was measured using Qubit dsDNA HS assay kit (Cat. No. Q32854, Invitrogen, Carlsbad, California, USA) and quality was checked by Agilent DNA HS kit (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA yield was measured right after IVT using the Qubit RNA Broad Range Assay kit with the Qubit Fluorometer (Thermo Fisher, UK) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... DNA quantification in the spent medium of the donor was performed through fluorescent labelling with Quant-iT reagent (Invitrogen Qubit dsDNA HS Assay Kit, Thermo Fisher Scientific). Fluorescence was measured with Infinite M200 PRO (TECAN ...
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bioRxiv - Bioengineering 2019Quote: ... The tissues were dissolved in RIPA buffer and the total protein concentration was measured with a Pierce BCA protein assay kit (Thermo Fisher Scientific). Proteins (15 μg each ...
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bioRxiv - Plant Biology 2021Quote: ... RNA concentration and quality were assessed by fluorometry and electrophoresis using a Qubit™ 4 and Qubit RNA HS Assay Kit (ThermoFisher Scientific) and a TapeStation (Agilent).
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bioRxiv - Plant Biology 2020Quote: ... RNA quality and concentration were assessed using a NanoDrop™ 1000 spectrophotometer and a Qubit 4 Fluorometer with an RNA HS Assay Kit (ThermoFisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Purified DNA from each sample was first quantified using a Qubit 3.0 fluorometer with a Qubit dsDNA Assay kit (Life Technologies, Carlsbad, CA). For the inhibition test ...
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bioRxiv - Molecular Biology 2020Quote: ... The biotinylated DNA fragments were applied to the gel-shift assay by using the LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, US) and Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was then carried out using the corresponding TaqMan™ Gene Expression Assay Kit that include exonspanning probes for genes of interest (ThermoFisher Scientific) (product numbers listed in Supplemental Table 5 ...
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bioRxiv - Biochemistry 2021Quote: ... and an equal amount of protein was used for labeling by Alexa Fluor 488 using the Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit (ThermoFisher). Labeled proteins were resolved on 12% SDS gel and visualized on a ChemiDoc Imager (BioRad) ...
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bioRxiv - Cancer Biology 2020Quote: Cell cycle analysis was performed using the Click-iT™ Plus EdU Alexa Fluor™ 647 Flow cytometry assay kit (Thermofisher Scientific). To obtain the cell cycle profile ...
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bioRxiv - Cell Biology 2020Quote: ... was solubilized with 0.1 N NaOH and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA). Cellular cholesterol content data was expressed as mg cholesterol/mg of protein ...
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bioRxiv - Cell Biology 2020Quote: ... Here after we followed the instructions provided by the manufacturer (PrimeFlow™ RNA assay kit, Cat no 88-18005-210, eBioscience/Thermo Fisher). In brief the samples were incubated with the first fixation buffer (PrimeFlow fixation buffer 1 ...
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bioRxiv - Biophysics 2020Quote: ... For more accurate characterization protein concentration was determined by the bicinchoninic acid method31 with the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). A method based on pyridine hemochrome spectra32 was used to determine the amount of heme ...
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bioRxiv - Cancer Biology 2019Quote: ... Click reaction was performed according to the manufacturer instructions (Click-iT™ Plus EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit; Invitrogen) and cells were analysed by flow-cytometry (BD FACSCanto II) ...