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Citations for Thermo Fisher :
8401 - 8450 of 10000+ citations for EnzyFluo ERK Phosphorylation Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... DNA quality and quantity were analyzed using a NanoDrop1000 spectrophotometer and a Qubit double- stranded BR assay kit on a Qubit fluorometer (Thermo Fisher, Germany).
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bioRxiv - Plant Biology 2023Quote: ... The quality and concentration of the extracted RNA were evaluated using a BioSpec-nano (Shimadzu, Japan) and Qubit Quantification Platform RNA BR Assay Kit (Invitrogen, United States). The extracted RNA was sent to Macrogen (Korea ...
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bioRxiv - Biochemistry 2023Quote: ... HM and IF pooled EV fractions were assayed for total protein according to manufacturer’s instructions (Micro BCA protein assay kit, Thermofisher Scientific, Brisbane, Australia) and a volume equivalent to 20 µg total protein was aliquoted for western blot (WB ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were performed on a 100-μl scale in a 96-well plate according to the “Amplex™ Red Cholesterol Assay Kit” protocol (ThermoFisher Scientific). No cholesterol esterase was added ...
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bioRxiv - Neuroscience 2022Quote: Total protein content in the PBS-soluble (clarified 10% brain homogenate) and detergent-soluble fractions was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) following the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... samples were centrifuged at 3000g for 10 minutes and the DNA concentration was measured by Qubit dsDNA HS assay kit (Life Technologies, Q32851). Briefly ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The PCR products were purified with AMPure XP beads and then were validated using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) for DNA concentration ...
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bioRxiv - Cell Biology 2022Quote: ... MSC-EVs were not lysed in RIPA buffer and protein content was determined via the Micro BCA™ Protein Assay Kit (ThermoFIsher, 23235).
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bioRxiv - Cancer Biology 2023Quote: ... spun at 13,000 rpm for 30 minutes at 4°C and supernatant protein concentration determined using the BCA assay kit (#23225; Thermo Fisher Scientific). 30-50 μg aliquots of lysate proteins were resolved on sodium dodecyl sulfate-7.5% polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Genetics 2022Quote: ... A protein concentration of the tissue lysates was determined with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) and 3-10 µg of the total protein lysate/homogenate used per assay well in duplicates and repeated in three independent runs ...
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bioRxiv - Developmental Biology 2023Quote: OPP staining was performed using a Click-iT Plus OPP Alexa Fluor 488 Protein Synthesis Assay Kit (ThermoFisher Scientific; Cat. No. C10456). Mouse embryos were first cultured in vitro from E1.5 to E2.5+7h in KSOM+AA as described above ...
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bioRxiv - Genomics 2023Quote: ... at a 1.6:1 volumetric bead-to-DNA ratio and quantification using the dsDNA High Sensitivity Assay kit on a Qubit® fluorometer 3.0 (Thermo Fisher Scientific), samples were diluted to 0.5 nM in a total of 24 μl and used as input for library preparation following the PCR barcoding (96 ...
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bioRxiv - Genomics 2023Quote: All PCR products were assessed by gel electrophoresis and quantified using the Qubit™ dsDNA HS Assay kit (Cat. #Q32854; Thermo-Fisher Scientific). Primers were synthesized by Integrated DNA Technologies® and diluted to 10uM in 0.1x TE buffer ...
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bioRxiv - Genomics 2023Quote: ... The DNA purity of samples was evaluated with a NanoDrop spectrophotometer and the concentrations were quantified using Qubit dsDNA HS Assay Kit with Qubit™ 4.0 (Invitrogen, CA, USA).
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bioRxiv - Genomics 2023Quote: ... Library quality control was performed by measuring concentration with Qubit® dsDNA HS Assay Kit (Thermo Fisher Scientific Inc., Cat. No. Q32854) and fragment size distribution with the Agilent® High Sensitivity DNA Kit (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... DNA concentration (minimum of 80 ng/µL) was measured using Qubit DNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA). For PacBio sequencing ...
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bioRxiv - Plant Biology 2023Quote: High molecular weight genomic DNA was extracted from young leaves of greenhouse-grown plants using the CTAB method,34 and concentration determined by Qubit dsDNA HS assay kit (Invitrogen, Waltham, MA). For each of the samples ...
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bioRxiv - Immunology 2023Quote: ... Click Chemistry labelling was performed according to Click-iT™ Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit (ThermoFisher, cat: C10458). The percentages of mitochondrial dependence ...
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Variation of wine preference amongst consumers is influenced by the composition of salivary proteinsbioRxiv - Biochemistry 2023Quote: ... protein concentration was standardised to 20 μg/mL according to the protein quantification results tested by the Micro BCA protein assay kit (Thermo Fisher Scientific). Reduction was carried out using 10 μL TCEP was added followed by incubation at 37 °C for 45 min followed by alkylation with 55 mM iodoacetamide ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenates were cleared by centrifugation at 13,000 x g at 4°C for 25 min and the protein concentration in the collected supernatant was measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). Protein extracts (20 µg of protein for each sample) ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations from PBS-soluble (soluble) and sarkosyl-soluble (insoluble) fractions were assessed using the BCA method (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific). Total protein lysates from PBS-soluble fractions and sarkosyl-soluble fractions (50µg ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were then centrifuged at 10,000 rpm for 10’ at 4°C and the supernatant was collected to determine protein concentration using Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Before loading ...
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bioRxiv - Plant Biology 2023Quote: ... The quality and quantity of the purified cDNA were determined using a Qubit 3 Fluorometer with a Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and an Agilent 2100 BioAnalyzer with a high-sensitivity DNA chip (Agilent Technologies) ...
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bioRxiv - Genomics 2023Quote: ... and DNA concentration was measured using a Qubit® DNA Assay Kit in a Qubit® 4.0 Fluorometer (Life Technologies, CA, USA). DNA integrity was checked by pulsed field gel electrophoresis (PFGE) ...
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bioRxiv - Immunology 2023Quote: ... a Luminex assay using the ProcartaPlex™ Mouse Cytokine & Chemokine Panel 1 36-plex kit (#EPXR360-26092-901, ThermoFisher Scientific, Waltham, MA) was performed according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... including the quantification of EV single particles and EV size diameter by the Flow Nanoanalyser56 (nanoFCMTM) and/or by measuring the total sterol amount by Amplex™ Red Cholesterol Assay Kit (ThermoFisher, A12216), following manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... Aliquots of SAGs were transferred to replica plates for DNA yield quantification using Quant-iT dsDNA Broad-Range (BR) Assay Kit (Thermo Fisher Scientific) prior to library preparation ...
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bioRxiv - Microbiology 2023Quote: ... Then each sample was diluted in a 1:10 ratio and measured by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentrations from PBS-soluble (soluble) and sarkosyl-soluble (insoluble) fractions were both assessed using the BCA method (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific). Total protein lysates (50μg ...
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bioRxiv - Cell Biology 2023Quote: ... were treated with 10 µM EdU for 6 hours before dissociation and staining using the Click-It EdU Alexa Fluor 488 Flow Cytometry Assay kit (ThermoFisher, Cat# C10425).
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bioRxiv - Developmental Biology 2023Quote: ... Extracted DNA and RNA were quantified using a Qubit dsDNA BR (Broad Range) Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA, #Q32853) and a High Sensitivity RNA assay kit (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... ratios using the Dual Luciferase Reporter Gene Assay Kit (RG027, Beyotime, Shanghai, China) with a Multimode Reader (Varioskan LUX, Thermo Fisher, Finland). Five biological replications were measured for each sample.
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bioRxiv - Genomics 2023Quote: ... The concentration of DNA for each of the three pools was measured via the Qubit dsDNA HS Assay kit (ThermoFisher, Cat: Q33230). Libraries were prepared for each pool ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected after centrifugation (12,000 x g, 20 min) and total protein levels were measured using Pierce™ BCA Protein Assay Kits (Thermo Scientific #23227). Proteins were denatured by boiling with Laemmli buffer (Bio-Rad #1610747 ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with O-propargyl-puromycin (OPP) using Click-iT® Plus OPP Alexa Fluor® 488 Protein Synthesis Assay Kit (Invitrogen) according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2023Quote: ... then protein concentration was determined using the Pierce™ BCA Protein Assay Kit as per manufacturer’s instructions (cat:23225, Thermo Scientific™). Samples were then processed using the S-trap protocol as per the manufacturer’s instructions54 ...
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bioRxiv - Cell Biology 2023Quote: ... protein concentrations were measured with the Pierce BCA protein assay kit coupled with the bovine serum albumin standards (23225, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 μl of the 12 mM MTT stock solution from the Vybrant® MTT cell proliferation assay kit (#V13154, Thermo Fisher Scientific), and vehicle or 200 μM DEM at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Salts were removed from the supernatant and collected in new reaction microtubes using self-packed C18 STAGE-tips.Peptide concentrations were finally determined with the Micro BCA™ Protein Assay Kit (Thermo Fisher Scientific). 200ng of each sample were analyzed by a LC-MS/MS system consisting in a nanoElute system (Bruker Daltonics GmbH ...
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bioRxiv - Cell Biology 2023Quote: ... EdU labeling was performed using the manufacturer’s protocol for the Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen, catalog no. C10420). EdU incorporation was measured by flow cytometry using the Attune flow analyzer ...
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bioRxiv - Biochemistry 2023Quote: Protein concentration in venom and antivenom samples was measured using Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Luciferase assays were performed on aliquots of cleared media harvested from transfected cells using the Pierce Gaussia and Cypridina luciferase flash assay kits (catalog numbers 16159 and 16169 respectively; Thermo Fisher Scientific) and a GloMax 20/20 luminometer (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... The binding affinity of mRNA at two pH values was measured using the Quant-it Ribogreen Assay Kit (Thermo Fisher Scientific, USA).
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bioRxiv - Biochemistry 2023Quote: ... Insoluble fraction was removed by 4°C centrifugation at 12000xg for 10 minutes and protein concentration in the soluble fraction was measured by PierceTM BCA protein assay kit (Thermo Fisher Scientific). Protein concentration was adjusted to 1-2 mg/mL for LDBIA treatment.
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bioRxiv - Biophysics 2023Quote: In vitro cytotoxicity of the complexes was evaluated using U-2 OS cells and an LDH assay kit.42 Cells were counted with a Countess II Automated Cell Counter (ThermoFisher Scientific, USA) and seeded in 96-well plates at a density of 10,000 cells per well in 100 μL of full DMEM ...
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bioRxiv - Genomics 2023Quote: ... SYBR Green assay: PCR reactions were prepared using the Power SYBR Green RNA-to-CT 1 step kit (Applied Biosystems, Cat# 4391112). Reactions contained a 2× Power SYBR Green RT-PCR Mix ...
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bioRxiv - Neuroscience 2023Quote: ... the protein concentrations of synaptosome samples resuspended in HEPES buffer were tested by the Pierce BCA Protein Assay Kit (Thermo Fisher #23225) following the manufacturer’s instructions and then diluted to 1 µg/µL ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA was eluted in 60 µL Elution Buffer and quantified using the Qubit fluorometer and the Qubit dsDNA HS Assay Kit (Invitrogen, Catalog #Q33231) following the recommended protocol.
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bioRxiv - Cell Biology 2024Quote: ... purity and length of the total genomic DNA was assessed using a Thermo Fisher Scientific Qubit 4.0 fluorometer with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854), a DeNovix DS-11 FX spectrophotometer and an Agilent FEMTO Pulse System with a Genomic DNA 165 kb Kit (Agilent ...