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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Tissue sections were blocked for 30 min in a blocking buffer (containing 5% horse serum (Thermo Fisher Scientific) and 1% Triton X-100 in PBS) ...
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bioRxiv - Microbiology 2022Quote: ... the RACE adapter (see Table S2) was ligated to 1-5 µg RNA using T4 RNA ligase (Invitrogen) for 1 hour at 37 °C or 2 hours at 25 °C ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-Mouse IgG conjugated with Alexa Fluor™ Plus 488 (5 μg/mL final concentration, Invitrogen A32723), and 10 μM Hoechst 33342 for 120 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Infection was observed after 5 days by measuring GFP fluorescence with a Cellomics ArrayScan HCS reader (Thermo Scientific). The percentage of GFP+ cells was calculated using Cellomics Scan software with identical settings for all wells analysed in a single experiment ...
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bioRxiv - Molecular Biology 2022Quote: Cells obtained after enzymatic digestion of testis were resuspended in 5-10 ml FACS buffer (1x DPBS [Gibco] supplemented with 1% Pen-Strep ...
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bioRxiv - Cell Biology 2022Quote: ... The TAP was removed and replaced with 5 mL of Max Efficiency Transformation Reagent for Algae (Invitrogen, 100021485). This was repeated 2 times ...
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bioRxiv - Microbiology 2022Quote: ... and BSR T7/5 cells (RRID:CVCL_RW96) were grown in in Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2022Quote: Hela cells were seeded in 6-well plates with cover slips and grown to 50% confluency at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (12430047, Invitrogen) supplemented with 10% heat-inactivated FBS (A4766801 ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (FBS, Cytiva #SH30071.03) and 1X Antibiotic-Antimycotic (Gibco #15240-062). VeroE6/TMPRSS2 (JCRB #1819 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Sections were washed 3 times 5 minutes in PBS and mounted using ProLong Gold mouting media (Thermo Fisher) under glass cover slips.
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bioRxiv - Molecular Biology 2022Quote: ... pH 7.3) was added in a buffer exchange and 5 μM Fura-4F AM (catalog number F14175, Invitrogen) was added to the myofibers for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...
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bioRxiv - Developmental Biology 2022Quote: ... A total of 5 μg DNA was immunoprecipitated with preblocked protein A/G Dynabeads (Thermo Fisher Scientific, MA) complexed to 2.5 μg antibody (anti-CTCF ...
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bioRxiv - Cell Biology 2022Quote: ... Isolated BM cells were maintained at 37 °C and 5% CO2 in DMEM (4.5 g/L glucose, Gibco) supplemented with 10% heat- inactivated fetal calf serum (FCS) ...
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bioRxiv - Neuroscience 2023Quote: ... Capillaries were filled with 5-10 μL of dye solution of 10 μg/μL DiD (Thermo Fisher Scientific) in 100% ethanol and the tip was approached to the bristle socket with a micromanipulator ...
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bioRxiv - Cell Biology 2024Quote: ... Minced tissues were digested at 37°C in an enzymatic digest buffer containing 5 mg/mL Dispase (Invitrogen), 2 mg/mL DNase I (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% fetal bovine serum (FBS) (Cytiva, SH30396-03) and 5% penicillin-streptomycin (pen-strep) (Gibco, 15140163) or DMEM with 4.5 g/L D-glucose ...
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bioRxiv - Cell Biology 2024Quote: ... PI (propidium iodide) uptake was measured after 5 min incubation with 100 μg/mL diluted PI solution (ThermoFisher) in complete medium at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The probe shank was coated before recordings by dipping 10 times every 5 s in DiI (V22885, ThermoFisher) for post hoc histological assessment of electrode tracks ...
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bioRxiv - Molecular Biology 2023Quote: ... The mobile phase consisted of buffer A: 5 mM ammonium acetate in water (LC/MS grade, Thermo Scientific) and buffer B ...
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bioRxiv - Biophysics 2024Quote: HEK293 cells were maintained at 37 °C in 5% CO2 containing Dulbecco’s modified Eagle’s medium (DMEM, purchased from Thermofisher) supplemented with 10% fetal bovine serum and penicillinstreptomycin (0.2 mg/ml) ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 15 μL of protein sample was supplemented with 5 μL of 4X NuPAGE LDS Sample Buffer (Thermo Scientific). Samples were incubated at 95°C for 10 minutes before loading on a 4-12% SurePAGE™ Bis-Tris precast gels (Witec AG) ...
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bioRxiv - Biochemistry 2024Quote: ... Off-line fractionated samples (5 μl aliquot of 15 μl sample) were injected via an autosampler (Thermo Scientific) onto a 5 μl sample loop which was subsequently eluted onto an Acclaim PepMap 100 C18 HPLC column (75 μm x 50 cm ...
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bioRxiv - Biochemistry 2024Quote: ... the cells (5×105 cells per well) were incubated in six-well plates (Thermo Fisher Scientific, Nunclon Delta) for 2 h before stimulation with 100 ng/mL lipopolysaccharide (from E ...
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bioRxiv - Cancer Biology 2024Quote: ... then lysed with 1ml of polysomal buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1.25 mM MgCl2, 5 U/ml RNase inhibitor [Invitrogen] ...
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bioRxiv - Cancer Biology 2024Quote: ... Coverslips were washed 5 times in PBS and mounted with ProLong Gold Antifade Mountant with DAPI (Invitrogen, P36931) and imaged on the VS200 or an Echo Revolve microscope.
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bioRxiv - Cancer Biology 2024Quote: ... T cells were stimulated for 5 days with Dynabeads Human T-Activator CD3/CD28 (Life Technologies, Carlsbad, CA) at a bead-to-cell ratio of 3:1 ...
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bioRxiv - Biophysics 2024Quote: ... which is the same medium as above with 5 μg·ml-1 hygromycin B (Thermo Scientific Chemicals, J60681-MC) replacing the zeocin.
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Cell Biology 2024Quote: ... SEM images were acquired on a Helios 5 UX DualBeam FIB-SEM platform (Fisher Scientific, Brno, Czech Republic) using SEM imaging mode at 5 kV and 0.1 nA using TLD and ICD detectors to image secondary electron and backscatter electron signal ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with PBS and incubated 5 minutes with PBS containing 2 µg/mL Hoechst (33342, Fisher Scientific, UK) and washed in PBS and briefly in ddH2O then mounted on glass slides using ProLong GoldTM anti-fade glass mounting medium (P36980 ...
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bioRxiv - Microbiology 2024Quote: Human cervical carcinoma cell line HEp2 cells (ATCC® CCL-23) were cultured at 37°C with 5% atmospheric CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were subjected to a 5-minute wash with buffer (0.35% Triton-X, Thermo Fisher PRH5142, in PBS) and permeabilized with 0.5% Triton-X in PBS for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... Huh7 cells were cultured in the same incubator conditions (37 °C, 5% CO2) in low glucose DMEM (Gibco) media supplemented with 10% FBS (Gibco 26140095) ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T cells were cultured in a controlled humidified incubator at 37 °C and 5% CO2 in DMEM (Gibco) media supplemented with 10% FBS (Gibco 26140095) ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... or BA.5 (2 μg/mL) spike proteins were coated onto 96-well Maxisorp plates (ThermoFisher, Cat. #439454) in 50 μL of 50 mM Na2CO3 pH 9.6 overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Plant Biology 2024Quote: ... The sections were cut to a thickness of 5 μm using a Manual Rotary Microtome (Thermo Fisher Scientific). Sections were incubated with the primary anti-FtsZ2-1 antibody (1:3500 dilution) ...
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bioRxiv - Neuroscience 2024Quote: ... each animal was injected intraperitoneally with 37 mg/kg BW of 5-ethynyl-2′-deoxyuridine (EdU, ThermoFisher, A10044) at 48 hours after surgery ...
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bioRxiv - Molecular Biology 2024Quote: ... 5-30 µg of lysates were resolved on pre-cast 10% or 12% Bis-Tris NuPAGE gels (Invitrogen), transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Plant Biology 2024Quote: ... perforatum and 5 μg was transcribed into cDNA using the SuperScriptTM IV First-Strand Synthesis system (ThermoFisher, Invitrogen). Candidate gene ORFs were PCR-amplified (Table S17 ...
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bioRxiv - Plant Biology 2024Quote: ... perforatum and 5 μg was transcribed into cDNA using the SuperScriptTM IV First-Strand Synthesis system (ThermoFisher, Invitrogen). Candidate gene ORFs were PCR-amplified (Table S17 ...
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bioRxiv - Neuroscience 2023Quote: ... The reaction was stopped by adding 5 milliliters of HBSS lacking Ca2+and Mg2+ (Gibco, catalog no. 14175095). The dissociated neuron resuspension was then centrifuged for four minutes at 200 rcf ...
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bioRxiv - Neuroscience 2024Quote: ... with 10 ng cDNA input in triplicates on a QuantStudio 5 real-time PCR system (Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table S1 ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa and COS-7 cells were seeded onto dishes coated with 5 mg fibronectin (Life Technologies 33016-015) in diH2O ...
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bioRxiv - Developmental Biology 2024Quote: ... pelleted by centrifugation and resuspended in 5 ml DMEM + 10% FBS + Pen/Strep/Glutamine (Invitrogen Cat# 10378-016). Cells were plated to achieve the greatest density possible depending on the number of cells available ...
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bioRxiv - Immunology 2024Quote: ... Isolated BM cells were maintained at 37°C and 5% CO2 in DMEM (4.5 g/L glucose, Gibco) supplemented with 10% heat-inactivated fetal calf serum (FCS) ...