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Citations for Thermo Fisher :
8101 - 8150 of 10000+ citations for 5 NITROFURFURYL ALCOHOL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... FAM122AID and FAM67-120 and variants were resuspended in ice-cold lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor tablet [ThermoFisher]), lysed by high-pressure cell homogenization (Avestin Emulsiflex C3) ...
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bioRxiv - Cell Biology 2023Quote: ... The blotted membrane was immersed in block buffer [5% milk (AppliChem # A0830.1000) in 1XTBS (TBS, Thermo Fisher #28358)] for 2 hr at RT ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were passaged every 1-2 weeks by incubating organoids for 5-10 min in TrypLE Express (Gibco) at 37℃ and mechanical disruption to make organoids fall apart ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 mM EDTA) containing 250 mM NaCl and Halt Protease and Phosphatase Inhibitor Cocktail (78445, Thermo Fisher Scientific) for 30 min at 4°C with rotation ...
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bioRxiv - Immunology 2023Quote: ... at 95°C for 5 min and separated on a 4-12% Bis-Tris gel (NuPAGE, Thermo Scientific) for 60 min at 170V ...
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bioRxiv - Cell Biology 2023Quote: ... All slides were blocked with 5% (v/v) normal goat serum (NGS) (Cat. No. 016201, Thermo Fisher Scientific) in PBS at RT for 1□h and incubated with primary antibodies (PGR - Cell signaling Technology 8757S ...
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bioRxiv - Cell Biology 2023Quote: All cell lines were cultured at 37°C with 5% CO2 in DMEM/F12 media (ThermoFisher Scientific #31331028) supplemented with 10% Tetracyclin-free Fetal Bovine Serum (Dutscher #500101L) ...
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bioRxiv - Biochemistry 2023Quote: ... Off-line fractionated samples (5 μl aliquot of 15 μl sample) were injected via an autosampler (Thermo Scientific) onto a 5 μl sample loop which was subsequently eluted onto an Acclaim PepMap 100 C18 HPLC column (75 μm x 50 cm ...
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bioRxiv - Cancer Biology 2023Quote: ... CaSki cells were grown at 37°C and 5% CO2 in RPMI-1640 high glucose medium (Gibco, #11965092) supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... and the fluorescence signals were traced by the quantitative real-time PCR QuantStudio 5 (Thermo Fisher Scientific, USA) with λex = 495nm and λem = 520nm ...
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bioRxiv - Genetics 2023Quote: ... Quantitative RT-PCR reactions were carried out on a Real-time PCR machine (QuantStudio 5 by Applied Biosystems), according to a protocol from the Bimake SYBY Green Master Mix qPCR Kit (B21402) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 5% 2-mercaptoethanol] and was resolved by SDS-PAGE on 4% to 12% Bis-Tris gels (Invitrogen). Proteins were transferred to a polyvinylidene difluoride immobilon TM-P membrane (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... followed by two 5-min washing steps with washing buffer B (2X SSC and 10% formamide, Ambion; AM9342). In a humidified chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell experiments were performed in RPMI or DMEM media supplemented with 5% charcoal stripped FBS (cFBS) (Gibco) as a method of removing substantial amounts of lipophilic materials from the media [17 ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 µl of a 300 µM intermediate concentration of DAPI (Invitrogen by Thermo Fisher Scientific, Cat. no.: D1306) was added per mL of sample ...
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bioRxiv - Cancer Biology 2023Quote: The human melanoma A375 cell line (ATCC) was cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle’s medium (DMEM; Gibco) supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... cell pellets were resuspended in ice-cold lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor [ThermoFisher]) and lysed by high- pressure cell homogenization (Avestin Emulsiflex C3) ...
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bioRxiv - Biochemistry 2023Quote: Cell pellets expressing PP2Aa9-589 were resuspended in ice-cold lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor tablet [ThermoFisher]), lysed by high-pressure cell homogenization (Avestin Emulsiflex C3) ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets were resuspended in lysis buffer (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor [ThermoFisher]), lysed by high-pressure cell homogenization (Avestin C3-Emulsiflex) ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets expressing EGFP_Nanobody were resuspended in ice-cold lysis buffer (50 mM Tris pH 8.0, 500 mM NaCl, 5 mM Imidazole, 0.1% Triton X-100, EDTA-free protease inhibitor tablet [ThermoFisher]), lysed by high-pressure cell homogenization (Avestin Emulsiflex C3) ...
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bioRxiv - Immunology 2023Quote: ELISA plates (Microtitre) were coated with 50 µl/well of 5 µg/ml NeutrAvidin (Thermo Fisher Scientific, #31000) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was precipitated overnight at −20 °C with 0.025 V of 5 mg/mL glycogen (ThermoFisher Scientific), 0.1 V of 3.0 M NaOAc (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... 2 x 5 min with 0.05 M PB and mounted on Superfrost Plus (ThermoFisher Catalog# 12-550-15) microscope slides ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA was precipitated at −20 °C overnight with 0.025 V of 5 mg/mL glycogen (ThermoFisher Scientific), 0.1 V of 1.0 M NaOAc (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein eluates were reduced and alkylated in the same step with 5 mM TCEP (Thermo Fisher Scientific, PG82080) and 20 mM chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Microbiology 2023Quote: ... 1 ml of mid-exponential growth phase cells (OD600 of 0.6-0.8) was mixed with 5 µg/ml Nile red (Invitrogen) and 1 µg/ml Hoechst 33342 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... PCR was performed in 50 µL system including 10 µL 5× HF buffer (Thermo Fisher Scientific, Vilnius, Lithuania), 1 µL dNTPs (10 mM ...
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bioRxiv - Immunology 2023Quote: ... 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (Thermo Fisher Scientific, cat.: 34042) was added and the reaction was terminated after 5 min under running tap water ...
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bioRxiv - Microbiology 2023Quote: ... The native genomic locus of Bacillus cereus B4264 Shedu (NCBI accession NC_011725.1, bases 954720-956931) 5 was synthesized (GeneArt/Invitrogen) and cloned into pSG1 vector between AscI and NotI sites ...
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bioRxiv - Molecular Biology 2023Quote: The RNA of 500 µL iRBCs at 3-5% parasitaemia was isolated using 3 mL TRIzol reagent (Invitrogen) followed by phenol-chloroform phase separation ...
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bioRxiv - Bioengineering 2023Quote: ... Coverslips were counterstained with 20 nM Hoechst 33342 for 5 min in Cell Staining Buffer (Thermo Fisher Scientific) then mounted on slides with Vectashield Antifade Mounting Medium (Vector laboratories) ...
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bioRxiv - Physiology 2023Quote: ... with a 1:5 – 1:10 passaging rate onto cultureware coated with Geltrex (LDEV-Free, hESC-Qualified; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 4 g/L dextrose, 5.957 g/L HEPES, 0.05 g/L hypoxanthine, 5 g/L Albumax II, Invitrogen) supplemented with 200 mM L-glutamine ...
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bioRxiv - Genetics 2023Quote: ... 3-5 million cells or approximately 15 ug of DNA crosslinked with 2% PFA (Fisher Scientific F79-500) were used as input per reaction ...
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bioRxiv - Plant Biology 2023Quote: ... samples were centrifuged at 13,000 × g for 5 min and loaded into SDS-PAGE gels (Invitrogen, Cat. NP0323BOX). Separated proteins were transferred using transfer stacks (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... Nodule sections were stained in 1xPBS solution containing 5 µM SYTO13 dye (Thermo Fisher Scientific, Waltham, MA, USA) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked with 5% nonfat dry milk and immunoblotted with antibodies against pY397-FAK (Invitrogen 44-624G) or total FAK (Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies were diluted in 1xTBST + 5% milk and incubated with membranes for 45 minutes (1:100,000; Invitrogen). Membranes were then washed 3x in 1xTBST washes for 10 minutes ...
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bioRxiv - Genetics 2023Quote: ... 5 μl of PCR products were purified by ExoSAP-IT™ PCR Product Cleanup Reagent (Thermo Fisher Scientific) following manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA linker (5’-CACGACGCUCUUCCGAUCU-3’) was ligated to the polyA RNA with T4 RNA ligase (Thermo Scientific) to capture RNAs with a 5’ monophosphate (5’P) ...
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bioRxiv - Pathology 2023Quote: ... PrP-deficient RK13 cells (ATCC) were transfected with 5-10 µg of plasmid DNA using lipofectamine 3000 (Invitrogen). At 24 hours post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with DMNB-caged cAMP (4,5-Dimethoxy-2-Nitrobenzyl Adenosine 3’,5’-Cyclicmonophosphate, Molecular Probes, D1037) for at least 30 min prior to imaging at a final concentration of 1 mM ...
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bioRxiv - Genomics 2023Quote: ... a total of 5 mLs of Homogenization Buffer with Propidium Iodide (PI) (1.5 µM, Thermo Fisher cat. #P3566) and Calcien ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 5 min at 95°C and loaded onto a NuPAGE 4-12% Tris/Bis gel (ThermoFisher) with NuPAGE MOPS running buffer (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... in plating media (Neurobasal, Gibco; supplemented with 5% horse serum, Sigma; 2 mM GlutaMAX, Gibco; and 1xGS21, GlobalStem). 500,000 cortical neurons were plated per well of a 6 well plate pre-coated with PDL ...
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bioRxiv - Microbiology 2023Quote: ... Real time PCR assay was performed on Quant studio 5 real time PCR machine (Thermo Fisher Scientific, USA). Temperature profile employed is provided in Table S4.
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bioRxiv - Microbiology 2023Quote: Spinner-adapted L cells were grown in Joklik’s minimum essential medium (JMEM; U.S. Biological) supplemented to contain 5% fetal bovine serum (FBS; Gibco), 2 mM L-glutamine (Corning) ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated DNA was then enriched with 5 µL pre-washed Streptavidin C1 beads per sample (Life Technologies, 65001) in biotin binding buffer (5mM Tris-HCL pH 7.5 ...