Labshake search
Citations for Thermo Fisher :
8051 - 8100 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: ... Elution was performed in nuclease-free water and 1 μL was used for measuring the concentration with the Qubit™ dsDNA High Sensitivity Assay Kit (ThermoFisher Scientific), while the size was assessed with a High Sensitivity DNA chip on an 2100 Bioanalyzer.
-
bioRxiv - Developmental Biology 2020Quote: ... 1% Sodium Citrate pH6.0 (30 minutes at room temperature): Proteinase K (1/25 of stock solution from the Click-iT™ Plus TUNEL assay kit - Molecular Probes #C10617 for 30 minutes at room temperature) ...
-
bioRxiv - Immunology 2021Quote: ... were obtained using the respective negative selection kit (Miltenyi, Bergisch Gladbach, Germany) and were labeled with 1 μM of proliferation dye eFluor450 (ThermoFisher, Waltham, Massachusetts). Potential suppressive T cell populations were obtained from spleens of infected C57BL/6 mice at day 6 post-infection and sorted using a FACS Aria II (BD Bioscience ...
-
bioRxiv - Cancer Biology 2021Quote: ... Living cell numbers and metabolic activity were measured with an MTT assay or labeled for 1 h using the LIVE/DEAD® kit (ThermoFisher Scientific) containing 2 μM calcein and 4 μM EthD-1 (Ethidium homodimer-1) ...
-
ST6GAL1-mediated heterogeneity in sialic acid levels potentiates invasion of breast cancer epitheliabioRxiv - Cancer Biology 2020Quote: ... 1 μg of total RNA was reverse transcribed using Verso™ cDNA synthesis kit as per manufacturer’s protocol (Thermo Scientific, AB-1453). Real time PCR was performed with 1:2 diluted cDNA using SYBR green detection system (Thermo Fischer Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... DNA quality was assessed on a 1% agarose gel and quantified using the Quant-IT dsDNA Broad Range Kit (Thermo Fisher Scientific). Genomic DNA was sheared using a Covaris LE220 focused-ultrasonicator to a 450 bp mean insert size.
-
bioRxiv - Neuroscience 2019Quote: RNA was isolated from either the periventricular white matter or the adjacent gray matter of 5 ALSP patients and 5 control patients (see Supplemntal Table 1) using Trizol and cDNA was prepared using a Super Script III First Strand Synthesis kit (Invitrogen, Carlsbad, CA). Real time PCR was performed using SYBR Green in an Eppendorf Realplex II thermocycler ...
-
bioRxiv - Physiology 2021Quote: The concentrations of IL-1β in tissue extracts of ovaries and livers were determined using an IL-1 beta Pro-form Mouse Uncoated ELISA kit (88-8014-22; Thermo Fisher Scientific) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Similar amounts of RNA were reverse transcribed and amplified using the TaqmanTM RNA-to-CTTM 1-Step kit (Applied Biosystems, Waltham, MA). The following Taqman probes were used for HEK293 or U20S-derived RNA ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Colony PCR products were versified on 1% agarose gels stained with RedSafe™ (ChemBio) and then purified with GeneJET PCR purification kit (Thermo Scientific) and sequenced by SourceBioscience.
-
bioRxiv - Plant Biology 2020Quote: ... DNA-free total RNA (1 µg) was used to synthetize cDNA with the ThermoScript™ RT-PCR System kit (Invitrogen, Carlsbad, CA) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... viral RNA isolation was carried out from all samples using QIAmp Viral RNA Mini Kit followed by qPCR (Taqpath™ 1-step Master Mix, ThermoFisher Scientific). The libraries were prepared for WGS using ACTIC amplicon based sequencing kits from Qiagen as per manufacturer recommended protocol ...
-
bioRxiv - Microbiology 2021Quote: ... The digested product was then electrophoresed on 1% (w/v) agarose gel and purified from the gel slice using PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific). The purified product was subcloned into pACYC177 plasmid at DraIII-XhoI sites ...
-
bioRxiv - Microbiology 2021Quote: ... whereas the DNA-removing efficacy was evaluated using the Qubit 1× dsDNA HS assay kit (Thermo Fisher Scientific, Waltham, MA, United States).
-
bioRxiv - Microbiology 2020Quote: ... followed by click chemistry using EdC-labelled HSV-1 DNA and the Click-iT EdU Alexa Flour 555 Imaging Kit (ThermoFisher Scientific, C10638) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Two hundred microliters of 2 μM calcein AM/4 μM ethidium homodimer-1 in PBS (Live/Dead® Viability Kit, Invitrogen, USA) were added to the wells and plates incubated for 30 min in the dark ...
-
bioRxiv - Cancer Biology 2020Quote: ... cDNA synthesis was performed on 1 µg of RNA with the high-capacity cDNA reverse transcription kit (ThermoFisher Scientific, cat no: 4374966) and Applied Biosystems Veriti 96-well thermocycler.
-
bioRxiv - Cell Biology 2021Quote: ... HSC (Lin−Sca-1+c-Kit+CD150+CD48−) from wildtype C57BL/6 mice were sorted into 16-well chamber slides (Thermo Fisher Scientific) coated with RetroNectin (Takara Bio) ...
-
bioRxiv - Cell Biology 2020Quote: ... Figure 2 –figure supplement 1) and by analyzing endotoxin levels with Pierce Chromogenic Endotoxin Quant Kit according to the manufacturer’s guidelines (Thermofisher, Waltham, MA, USA) using TECAN Infinite M200 PRO (Männedorf ...
-
bioRxiv - Cell Biology 2020Quote: ... The extracted DNA (1 ng) was used for quantitative PCR with the TaqMan Universal PCR Master Mix kit (Thermo Fisher Scientific Incorporated) on a CFX connect real-time system (Bio-Rad Laboratories ...
-
bioRxiv - Cell Biology 2021Quote: ... polyadenylated mRNA was isolated from 1-3 µg of high-quality total RNA using the Dynabeads mRNA DIRECT Micro kit (Thermo Fisher Scientific). The mRNA was subsequently fragmented and used for cDNA synthesis and library preparation using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina ...
-
bioRxiv - Cell Biology 2021Quote: ... neurons were harvested for immunoblotting or incubated with calcein-AM and ethidium homodimer-1 (LIVE/DEAD™ Viability/Cytotoxicity Kit, Thermofisher, L3224), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Total RNA (1 μg) was reverse transcribed with random hexamers using Hight-Capacity cDNA Reverse Transcription Kit with Rnase Inhibitor (Applied Biosystems, ThermoFisher Scientific) following the manufacturer’s protocol ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... cDNA was synthesized from 1 μg of total RNA using a High Capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA) in a final volume of 20 μL ...
-
bioRxiv - Neuroscience 2022Quote: RNA was isolated from the gray matter of 5 CRL patients and 5 control patients (see Supplemental Table 1) using Trizol and cDNA was prepared using a Super Script III First Strand Synthesis kit (Invitrogen, Carlsbad, CA). Real time PCR was performed using the PrimePCR CSF3 assay qHsaCED0043218 from BIO-RAD ...
-
bioRxiv - Biochemistry 2022Quote: ... 20 μg of the plasmid was transfected into 20 mL of Expi293F cells at 3 × 106 cells mL-1 using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... The amplified fragments were joined by overlap extension PCR and cloned into the EcoRI-XhoI sites of pCAG-1 using the GeneArt Seamless Cloning and Assembly Kit (Thermo Fisher Scientific), to yield pCAG-2HA-NRXN1β(+S4)-GPI ...
-
bioRxiv - Molecular Biology 2020Quote: ... The RNA fragments were synthesized using 1 μg of PCR-generated fragments using the TranscriptAid T7 High Yield Transcription Kit (Thermo Fisher Scientific). Upon DNase I treatment for 30 min (Thermo Fisher Scientific) ...
-
bioRxiv - Plant Biology 2021Quote: cDNA was synthesized from 1 µg of purified RNA of each samples using cDNA high yield synthesis kit (SuperScript IV VILO, Thermo Fisher Scientific). Quantitative PCR was performed with following primers and SYBR green kit (PowerSYBP Green PCR Master Mix ...
-
bioRxiv - Cell Biology 2021Quote: Purified Tq-Ca-FLITS was 4x diluted in calcium buffers containing 0 or 39 µM free calcium of the Calcium Calibration Buffer Kit #1 (C3008MP, Thermo Fisher Scientific). The absorbance spectra were measured before and > 5 min after addition of 1 M NaOH ...
-
bioRxiv - Cancer Biology 2020Quote: ... RT-PCR was carried out with 1 μg RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster city, CA), according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... were pated at 1×104 per well in NB/B27+ with iMg growth factors (B-27 Plus Neuronal system kit (Life technologies A3653401) + 25ng/mL M-CSF (peprotech 300-25 ...
-
bioRxiv - Immunology 2020Quote: Supernatants and cell pellets were collected from the interaction between IGRP-GFP CD8+ T-cells and βlox5 cells at a 10:1 ratio and were evaluated using a granzyme B ELISA kit (BMS2027; ThermoFisher Scientific, USA.). The standard curve was produced to determine the granzyme B concentration ...
-
bioRxiv - Immunology 2021Quote: ... IL-1-Ra plasma levels were determined of 20 patients positive and 12 patients negative for IL-1-Ra-Abs with a commercially available ELISA kit (Invitrogen/ThermoFisher #BMS2080) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... First-strand cDNA was synthesized from 1 μg total RNA using the SuperScript III Reverse Transcriptase enzyme kit (Invitrogen, Carlsbad, CA, USA). Samples were labeled for microarray analysis using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp WT Labeling kit (Agilent Technologies ...
-
bioRxiv - Immunology 2022Quote: ... were transfected with plasmids carrying J08 and 02M04 antibody heavy and the light chains with a 1:2 ratio using ExpiFectamineTM293 Transfection Kit (Thermo Fisher Scientific) as recommended by the manufacturer ...
-
bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
-
bioRxiv - Microbiology 2019Quote: ... All of the cornea cells and a portion of the spleen cells were each incubated at 1:1000 of Live/Dead Fixable Aqua Dead Cell Stain Kit (Thermo Fisher Scientific) in PBS in the dark at RT for 30 minutes ...
-
Lateral organ diversification in plants mediated by the ALOG protein family of transcription factorsbioRxiv - Plant Biology 2019Quote: Gemmalings were incubated in 1/2 B5 medium containing 10uM EdU (Click-iT Edu Alexa Fluor 488 imaging kit; Thermo Fisher, USA) for 3h ...
-
bioRxiv - Zoology 2020Quote: ... The cells were transiently transfected with ∼1 μg of vector using Lipofectamine 3000 Reagent kit according to the manufacturer’s recommendations (Thermo Fisher Scientific, America) when the density became 60%–70% in a 6-well cell culture plate ...
-
bioRxiv - Neuroscience 2019Quote: ... Free-floating sections were initially washed 2×10min in PBS (7.4 pH) on an agitator before being transferred into Avidin solution (1:4000 in 0.3% PBS-T; Life Technologies NeuroTrace BDA Kit) overnight at 4°C on an agitator ...
-
bioRxiv - Microbiology 2019Quote: ... Table 1) and the sgRNAs were synthesized in vitro according to the specifications of the GeneArt Precision sgRNA Synthesis Kit (ThermoFisher Scientific, USA).
-
bioRxiv - Immunology 2019Quote: ... Reverse transcription of 1 µg RNA into cDNA was done using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA, USA). RT-PCR was performed on the LightCycler® 480 instrument using the LightCycler® 480 SYBR Green I Master mix (Roche Diagnostics ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... cDNA was synthesized from 1 μg total RNA in 20 μl reactions using High Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). After synthesis ...
-
bioRxiv - Molecular Biology 2020Quote: ... using the high sensitivity Next Generation S equencing kit 1-6000 (DNF-474) and the concentrations were measured on a Qubit Fluorometric Quantification system (Thermo Fisher Scientific), both according to manufaturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... RNA probes synthesized by T7-mediated transcription were purified by phenol-chloroform extraction and 1 mg RNA labelled with Alexa-546 using the ULYSIS Nucleic Acid Labeling Kit (Thermo Fisher (42). Nuclei were counterstained with Hoechst.
-
bioRxiv - Cancer Biology 2019Quote: ... on 1 μg of total RNA for each condition using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Saint Aubin, France) with random hexamer pdN6 primers.
-
bioRxiv - Bioengineering 2020Quote: ... RNA was quantified using spectrophotometry and 1 μg of RNA of each sample was reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific), following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Wild-type and CrPV-DcDV RNA was prepared by in vitro transcription of 1 µg linearized plasmid using the mMESSAGE mMACHINE T7 transcription kit (Invitrogen, Carlsbad, California) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells were treated for 1 hour with mock (untreated cells) or PNDD1 200 nM before fractionation with Subcellular Protein Fractionation Kit (#78840, Thermo Fisher Scientific) according to the manufacturer’s protocol ...