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Citations for Thermo Fisher :
8001 - 8050 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Synthesis of cDNA was performed on 1 µg of DNAse-free RNA using the SuperScript VILO cDNA Synthesis Kit (Invitrogen, Thermo Fisher). Power SYBR Green PCR Master Mix (Invitrogen ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... 1 µg of total RNA was depleted of ribosomal-RNA transcripts using MICROBExpress Bacterial mRNA Enrichment Kits (Thermo-Fisher Scientific, USA). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... and cDNA samples were generated from 1 µg of total RNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems). The RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2023Quote: ... Single-stranded cDNA was synthesized from 1 μg of total RNA using the High Capacity RNA-to-cDNA Kit (Applied biosystems) after depleting genomic DNA ...
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bioRxiv - Microbiology 2023Quote: ... The extracted RNA was resolved in 50 μl pure water and measured by RT-qPCR using Power SYBR Green RNA-to-Ct 1-step Kit (Applied Biosystems). The pgRNA expression level was normalized against the expression level of GAPDH as a housekeeping gene and calculated by the ΔΔCT method ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed using 1 μg of DNase-treated RNA (TURBO DNA-free Kit) with TaqMan Reverse Transcription Reagents (Applied Biosystems). Real-time PCR was performed using SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... DNase treatment was given to the extracted RNA for 15 mins at RT using DNase-1 kit (cat no.18068-015, Gibco/Thermofisher scientific) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... 1 µg of eluted RNA was treated with 2 U of DNase using the Invitrogen Purelink DNase kit (ThermoFisher, Waltham, USA) and incubated at 37°C for 30 minutes followed by inactivation at 62°C for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... cDNA synthesis was performed using approximately 1 µg RNA was taken using the Revert Aid H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and quantitative real-time PCR were conducted using TaqMan RNA-to-Ct 1-step Kit and 7500 Fast Real-Time PCR System (both from Applied Biosystems) with the indicated primer and probe sets (Table S2) ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... 1 µg of RNA per sample was used for cDNA synthesis with the RevertAid H Minus First Strand Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... whilst for each mRNA sample 1 µg of total RNA was reverse transcribed using the High Capacity RT cDNA Kit (ThermoFisher Scientific). qRT-PCR analysis of miRNAs used the miRCURY LNA SYBR green kit (Qiagen ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplified products were treated with 0.4U/µl DpnI for 1 h at 37°C prior to their purification with the GeneJET PCR Purification Kit (Thermo Scientific). Blunt-end ligation was carried out with 100 ng of DNA ligated in 20 µl reactions with 40U/µl T4 DNA ligase ...
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bioRxiv - Microbiology 2024Quote: ... The dry pooled sample was resuspended in 1 ml of 5% formic acid and was desalted using the EasyPep Maxi Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Calcein AM and Ethidium homodimer-1 from the LIVE/DEAD® Viability/Cytotoxicity Kit for mammalian cells were applied following manufacturer instructions (Invitrogen). For a reference control ...
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bioRxiv - Neuroscience 2024Quote: ... mRNA-Seq library concentrations (mean of 5.36 ± 2.37 ng μl−1) were measured with the Qubit dsDNA HS Assay Kit (Invitrogen; Cat. No. Q32851) on a Qubit 2.0 Fluorometer ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated RNA (1 μg per sample) was used to program cDNA synthesis (SuperScript™ III One-Step RT kit, 12574-018 Invitrogen). Expression of pluripotency markers and determination of tri-lineage differentiation potential was evaluated using the TaqMan hPSC Scorecard™ Panel (A15876 ...
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bioRxiv - Microbiology 2024Quote: ... Real-time RT-PCR for ToBRFV was performed based on the protocol of the International Seed Health Initiative for Vegetable Seeds using the CaTa28 and CSP1352 primers and probes (ISF, 2020, EPPO, 2022) with two modifications: the TaqMan RNA-to-CT 1-step Kit (Applied Biosystems) was used instead of the Ultraplex 1-step Toughmix (Quantabio ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription reaction was conducted to obtain cDNA using 1 μg RNA and Maxima FirstStrand cDNA Synthesis kit (Thermo Fisher Scientific). Gene expression levels were quantified by performing quantitative real-time PCR using TaqMan Gene Expression Assays (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: 10 µl was immediately removed and diluted 1:10 into buffer containing dyes from the Invitrogen Live/Dead Sperm viability kit (Thermo-Fisher Scientific) to assess baseline sperm count and viability using a Nexcelom Cellometer (Nexcelom Bioscience ...
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bioRxiv - Physiology 2024Quote: ... Diluted spermatozoa were mixed by briefly vortexing and 10 µl of the spermatozoa sample was immediately diluted 1:10 into buffer containing dyes from the Invitrogen Live/Dead Sperm viability kit (Thermo-Fisher Scientific) to assess baseline sperm count and viability as for Array 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative reverse-transcriptase PCR was performed using Power SYBR® Green RNA-to-CT™ 1-Step Kit from ThermoFisher (4389986). All data were normalized to ACT1 transcript levels and are presented as ΔCt values ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from the cell homogenates using the Qiagen RNeasy Maxi kit followed by a 1 hour Turbo DNase treatment (Thermo Fisher) with 5 uL of DNase in 1475 uL total volume for 1 hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized from 1 µg of total RNA using high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA). A 1:10 diluted cDNA was used to detect P ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with LIVE/DEADTM Fixable Near-IR Dead Cell Stain Kit at a 1:1500 dilution (ThermoFisher, Cat#L34975) in FACS buffer (1% BSA in 1X PBS ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized utilizing 1 μg of each condition’s total isolated RNA via a High-Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, 4368814). A 1:10 dilution of cDNA was used to detect P ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg of total RNA was subjected to reverse transcription using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA). For quantitative real-time polymerase chain reaction (qRT-PCR) ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were co-transfected at a 1:2 heavy to light chain ratio into Expi293F cells using the Expifectamine 293 Expression Kit (Thermo Fisher), and antibodies were purified with protein A agarose (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR cloning kits from Thermo Scientific (TOPO TA Cloning Kit 450030) and New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... GeneJET gel extraction kit and plasmid miniprep kit (Thermo Fisher Scientific) were used for DNA extraction ...
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bioRxiv - Genetics 2021Quote: A commercially available kit (Click-iT EdU Cell Proliferation Kit, ThermoFisher) was used to label proliferating cells with thymidine analog 5-ethynyl-2’- deoxyuridine (EdU) ...
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bioRxiv - Microbiology 2021Quote: ... The commercially available kit (ThermoFisher, CyQUANT™ LDH Cytotoxicity Assay Kit) was used according to manufacturer protocol ...
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bioRxiv - Pathology 2023Quote: ... A magnetic bead sorting kit (CELLection Pan Mouse IgG Kit, Invitrogen) was used according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μg of total RNA was reverse transcribed using Verso™ cDNA synthesis kit as per manufacturer’s protocol (Thermo Scientific, AB-1453). Real-time PCR was performed with 1:2 diluted cDNA using SYBR green detection system (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2020Quote: ... Standard beads with a diameter of 1 µm (Flow cytometry Sub-Micron Size Reference Kit, Invitrogen, Life Technologies Corp., Carlsbad, CA, USA) were used for sizing calibration ...
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bioRxiv - Cancer Biology 2021Quote: ... and a 1:200 dilution of Molecular Probes Fixable Live/Dead Near-IR Dead Cell Stain Kit (Thermo Fisher Scientific, MA, USA). FACS was performed to isolate Calcein Blue-positive and L/D NearIR-negative cells into a precooled 1.5ml Eppendorf tube containing 750µL collection buffer consisting of Gibco RPMI 1640 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... A total of 1 μg RNA was reversely transcribed in a 20 μl reaction mixture using high-capacity cDNA reverse transcription kit (Applied Biosystems, #4368814) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1 µg of RNA was used for single strand cDNA synthesis with the SuperScript VILO cDNA Synthesis kit (Invitrogen, Thermo Fisher Scientific). Quantitative PCR was done using the TaqMan System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... 1 µg of RNA was used for single strand cDNA synthesis with the SuperScript VILO cDNA Synthesis kit (Invitrogen, Thermo Fisher Scientific). Quantitative PCR was done using the TaqMan System (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated and purified from cultured mouse TPCs (passage 1) with Trizol followed by complementary DNA synthesis with RevertAid™ H Minus kit (#K1632 Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Total RNA (1 μg) from each sample was used for reverse transcription with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Life Technologies). Real-time PCR was performed in ABI Step One Plus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... Dead cells were removed using Live/Dead Fixable Near-IR or Yellow Dead Cell Stain Kit (Invitrogen, L34976 or L34959, 1:500) in each panel ...
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bioRxiv - Genetics 2021Quote: ... The gDNA sample integrity was controlled on 1% agarose gel and quantified on Nanodrop and Qubit using the Qubit™ dsDNA BR Assay Kit (Invitrogen™). 2 × 2 µg of gDNA were digested in parallel with 50 units of DpnII (NEB #R0543L ...
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bioRxiv - Microbiology 2019Quote: ... using 100 ng ribosome RNA-depleted and barcoded t-RNA (SOLiD™ RNA Barcoding Kit, Module 1-16 (Life Technologies, PN 4427046) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... was added into 1 x 105 cells in 12 μl of R buffer in Neon® transfection system 10 μL Kit (Invitrogen, #MPK1096) and the cell suspension applied to Neon® transfection system with 10 Neon tips ...
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bioRxiv - Cell Biology 2022Quote: iPSCs were pulsed with 20 μM EdU for 1 hour using the Click-iT EdU Alexa Fluor 647 Flow kit (Thermo Fisher C10424). iPSC-CMs were pulsed with 10 μM EdU for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in a ratio of 3:1 and total RNA was extracted with Ribopure Yeast Kit (Ambion, Life Technologies) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Cancer Biology 2019Quote: ... 25 ng of RNA was used as starting material per sample and qPCR was performed using the Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) on a CFX Connect Real-Time light cycler (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... Reverse transcription and PCR amplification were performed using TaqMan RNA-to-CT 1-Step Kit and the StepOnePlus™ real-time PCR instrument (Applied Biosystems). The following probes were used in this study ...