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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: Single cells were stained with 4 μM Ethidium homodimer-1 and 2 μM Calcium AM (ThermoFisher LIVE/DEAD Viability Kit L3224) in the dark at room temperature for 20 min ...
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bioRxiv - Cancer Biology 2020Quote: M28 and BPH-1 cells were surface biotin-labeled with EZ-Link Sulfo-NHS-LC-Biotinylation Kit (Pierce/Thermo Fisher Scientific) and lysed with cell lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with EdU at a final concentration of 1 μM for 30 min before staining was carried out as detailed in Click-iT EdU Imaging Kits (Life Technologies). For co-localisation staining ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was homogenized in TRI-reagent using a Qiagen TissueLyser and 1 μg of RNA was reverse transcribed using High-Capacity cDNA Reverse Transcription kit (Life Technologies) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized from total RNA (1 μg) with random hexamers using a High Capacity cDNA reverse transcription kit (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: A total of 1 µg RNA was used for cDNA synthesis using a RevertAid First Strand cDNA synthesis kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Total RNAs were extracted using Trizol reagent and 1 μg of RNA was reverse-transcribed with the RevertAid H Minus First Strand cDNA Synthesis kit (Life Technologies). qPCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... Total RNA (1 µg) was reverse transcribed to cDNA using High Capacity cDNA Reverse Transcription Kits with random primers (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Estimation of viral RNA copy number in patients’ samples was performed using real-time RT-PCR (TaqMan RNA to-Ct 1-Step Kit; Applied Biosystems) with primers and probes ...
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bioRxiv - Plant Biology 2021Quote: ... RNA quality was examined on a 1% agarose gel and RNA concentration was quantified using the Qubit RNA HS assay kit (Invitrogen, USA). 2ug of total RNA was used to construct stranded RNA-seq libraries using the Illumina TruSeq stranded total RNA LT sample prep kit (RS-122-2401 and RS-122-2402) ...
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bioRxiv - Genomics 2020Quote: ... After priming the C1 array and loading of the cell mix we added a Calcein AM/ Ethidium homodimer-1 staining mix (LIVE/DEAD kit, Life Technologies). Both protocols follow the manufacturer guide to perform the cell mix loading ...
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bioRxiv - Immunology 2021Quote: ... 2 µl of the extracted RNA from WSN-infected or uninfected mouse lungs were analyzed by quantitative real time PCR (RT-qPCR) using the TaqMan RNA-to-Ct 1-Step Kit (Applied Biosystems). Ct values for the gene of interest and the housekeeping gene Gapdh (glyceraldehyde-3-phosphate dehydrogenase ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Cancer Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized using total RNA (1 μg per reaction) with SuperScript Vilo cDNA synthesis kit (Thermo Fisher Scientific) as per manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from DNAse-treated total RNA (1 μg) with reverse transcriptase and oligo-dT (High Capacity cDNA reverse transcription kit, Applied Biosystems). RT-qPCR was performed in optical 96-well plates using SYBR® green in a Light Cycler 480 (Roche) ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from DNAse-treated total RNA (1 µg) with reverse transcriptase and oligo-dT (High Capacity cDNA reverse transcription kit, Applied Biosystems). RT-qPCR was performed in optical 96-well plates using SYBR® green in a Light Cycler 480 (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized using 1 μg of total RNA with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Cat# 4368814) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Nuclei were sorted directly into tubes containing 3:1 by volume of the Extraction Buffer from PicoPure RNA Isolation kit (ThermoFisher Scientific). RNA was then extracted from the sorted nuclei using the PicoPure kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Viral RNA accumulation was estimated by real-time quantitative PCR (RT-qPCR) using the Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems) and following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: Digested PCR products and digested vector backbone were checked on a 1% agarose gel and purified using a GeneJET gel extraction kit (Thermo Fisher) or purified directly from the mix without running it on the gel using a GeneJET PCR purification kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... Complementary DNA (cDNA) was synthesized using 1 μg of input RNA and the High Capacity RNA-to-cDNA Kit (Thermo Fisher). Quantitative PCR was performed by standard protocol using diluted cDNA ...
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bioRxiv - Microbiology 2019Quote: ... RNA was converted to complementary DNA (cDNA) using 1 μg of input RNA in the High Capcity RNA-to-cDNA kit (Thermo Fisher). Quantitative PCR was performed using the standard protocol for SYBR Green reagents (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Complementary DNA (cDNA) was then synthetized from 1 μg of total RNA using the Revert Aid First Strand cDNA Synthesis Kit (Thermo Scientific), employing random hexamer primers ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesized from 1 μg of total RNA using Super Script III First Strand cDNA Synthesis Kit (Invitrogen, Carlsbad, CA) and random hexamer ...
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bioRxiv - Genetics 2020Quote: ... sequencing of each transcript was performed using the Big DyeR Terminator V1-1 Cycle Sequencing Kit (Life technologies, Carlsbad, CA, USA) on ABI Prism 3130xl capillary DNA Sequencer.
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bioRxiv - Bioengineering 2021Quote: ... injected into a petri dish and incubated with 2 µM calcein AM and 4 µM EthD-1 (Live/Dead Viability/Cytotoxicity Kit, Life Technologies) in PBS++ for 30 minutes at 37 ° C ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized using all RNA available (or 1-5 ng) with the High-Capacity Reverse Transcription Kit with RNase Inhibitor (Life Technologies) (250 C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... pUC57-T7-vaultRNA1-1 plasmids were linearized and used for in vitro transcription of RNA using the MEGAshortscript kit (AM1354, Thermo Fisher) with 32P-αUTP (SRP-210 ...
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bioRxiv - Cancer Biology 2020Quote: ... normal peripheral blood mononuclear cells and AML patient samples was performed using Taqman RT-qPCR RNA-to-Ct 1-Step Kit (Applied Biosystems). GAPDH housekeeping gene was quantified using pre-developped TaqMan human GAPDH endogenous control (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was quantified and 1 μg was used as starting material for reverse transcription using the Superscript IV cDNA synthesis kit (Life Technologies) following manufacturer’s instruction ...
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bioRxiv - Bioengineering 2022Quote: ... About 1 μg of RNA was used to synthesize cDNA with a RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Scientific). CDNA was diluted 50 times before use in Real-Time quantitative PCR (RT-qPCR) ...
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bioRxiv - Biochemistry 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was executed using 50 ng RNA in Power SYBR Green RNA-to-Ct 1-step Kit (Applied Biosystems). A CFX384 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with cold 1×PBS and the chromatin-bound proteins were extracted using the subcellular fractionation kit (Thermo Fisher). Protein concentrations were measured by the BCA assay kit (Thermo Fisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Amplified products were treated with 0.4U/μl Dpnl for 1 h at 37°C prior to the PCR purification with the GeneJET PCR Purification Kit (Thermo Scientific). 100 ng of DNA products were blunt-end ligated in 20 μl reactions with 40U/μl T4 DNA ligase in 1× T4 DNA ligase buffer overnight at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was retrotranscribed and amplified in an QuantStudio 7 Flex thermocycler (Applied Biosystem) using Taqman RNA-to-Ct 1-Step Kit (Thermofisher, 4392653). The Taqman probes used were the following Jag1 (Mm00496902_m1) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg of RNA was retrotranscribed into cDNA using Maxima H minus cDNA synthesis Master Mix with dsDNase kit (ThermoFisher Scientific). RTqPCR was performed using PowerUp™ SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... The DNA concentration of 1 μL of each sample was determined using the Qubit dsDNA HS Assay kit (Fisher Scientific, #33231) in black ...
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bioRxiv - Plant Biology 2022Quote: ... 1 ug of DNase-free RNA was used to synthesize cDNA with a RevertAid H Minus kit (EPO452, Thermo Fisher Scientific). A qRT-PCR was performed with a CFX Connect Real Time PCR machine (BioRad ...
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bioRxiv - Neuroscience 2022Quote: ... syp-mCh and SSF-tagged opioid receptors using the lipofectamine method were incubated for 15 minutes with Alexa-647 conjugated anti-FLAG antibody (1/1000, M1 antibody from Sigma, Alexa Fluor 647 Protein Labeling Kit from Thermo Fisher) before neurons were washed 3 times with HBS and mounted on the microscope in HBS ...
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bioRxiv - Immunology 2022Quote: ... PCR products were run on a 1% agarose gel and fragments of the correct length were extracted with Thermo gel extraction and PCR cleanup kit (ThermoFisher Scientific). Gel-extracted products were digested with DpnI (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: qRT-PCR was performed using the Power SYBR® Green RNA-to-CT™ 1-Step Kit (Thermo Fisher Scientific #4389986) on a StepOnePlus™ Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... GeneRuler 1 kb Plus DNA ladder and Quant-iT™ PicoGreen™ dsDNA Assay Kit were from Thermo Scientific (Waltham, MA). Size #3 hydroxypropyl methylcellulose Quali-V-I capsules were from Qualicaps (Whitsett ...
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bioRxiv - Plant Biology 2022Quote: ... and seedlings sprayed with mock (DMSO) or 1 μM BAP for 60 min (Fig. 1B) using RNAqueous™ Total RNA Isolation Kit (ThermoFisher).
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bioRxiv - Microbiology 2022Quote: ... and sgE were performed using previously-described primers and probes [40–42] and the TaqMan RNA-to-Ct 1-Step Kit (Thermo Fisher). Assays were performed on a QuantStudio 7 Pro real-time PCR system (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... 1% penicillin-streptomycin and 1% non-essential amino acids) as described previously (37) and transduced using CytoTune™ –iPS 2.0 Sendai Reprogramming kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Total RNA (1 µg) was reverse transcribed to cDNA using High Capacity cDNA Reverse Transcription Kits with random primers (Applied Biosystems). qPCR was performed using an ABI Real-Time PCR 7500 system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... MERS-CoV and REBOV were diluted to 2×10-3 and confirmed by their respective monoplex one-step RT-PCR with specific primers using the Verso 1-step RT-PCR kit (Thermo Scientific). The reaction was set up in 10 μl final volume with 100 nM each of unlabelled forward and reverse primers ...
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bioRxiv - Neuroscience 2022Quote: Clathrin and caveolin-1 knockdown in polarized hCMEC/D3 cell monolayers was performed using siRNA kit containing RNAi mix (Invitrogen, CA) and reduced serum medium ...