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Citations for Thermo Fisher :
7951 - 8000 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... qRT-PCR was performed using the TaqMan RNA-to-Ct 1-Step Kit and TaqMan gene expression assays (Thermo Fisher Scientific). PCR amplifications were performed in triplicate or quadruplicates (n = 4-5 animals per genotype ...
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bioRxiv - Physiology 2022Quote: ... Complementary DNA (cDNA) was prepared from 1 ug of total RNA using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) and the resulting cDNA was diluted 1:50 before use ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of extracted RNA was converted to cDNA using Superscript IV First-strand synthesis kit (ThermoFisher Scientific, catalog number 18091050). 1 μl of prepared cDNA (equal to 50 ng of template RNA ...
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bioRxiv - Microbiology 2022Quote: ... DNA extractions were diluted 1:10 and DNA concentration was measured using a Qubit dsDNA HS Assay Kit (Invitrogen, Waltham, USA). Depending on the concentration ...
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An SNF2 helicase-like protein links mitotic transcription termination to sister chromatid resolutionbioRxiv - Cell Biology 2022Quote: ... ICRF-193 (280 μM); mRNA for RNAi resistant lds (1 mg/ml; prepared using mMESSAGE mMACHINE T3 transcription kit (Thermo Fisher), using Lds ORF subcloned in pRNA vector ...
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bioRxiv - Physiology 2024Quote: ... Armenian hamster anti-mouse MXRA8-Alexa Fluor 647 (1:200, clone 9G2.D6, conjugated using the Alexa Fluor™ 647 Antibody Labeling Kit by Invitrogen), rat anti-mouse CD19-Spark NIR 685 (clone 6D5 ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized using a maximum of 1 μg of total RNA with the High-Capacity cDNA Reverse Transcription Kit (Cat# 4368814, Applied Biosystems). Quantitative RT-PCR was performed using the Taqman Fast Universal PCR Master Mix (Cat# 4352042 ...
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bioRxiv - Systems Biology 2024Quote: ... and 1 µg RNA starting material was reverse-transcribed with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Germany). Gene expression was analyzed with predesigned QuantiTect primer assays (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of RNA was reverse transcribed using the High-Capacity RNA-to-cDNA™ kit (Thermo Fisher Scientific, Cat# 4387406) following the protocol instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA concentration was determined using Equalbit 1 × dsDNA HS Assay Kit (Vazyme, Nanjing, China) and a Qubit 3.0 Fluorometer (Life Technologies, Singapore) before submission for whole-genome sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... Synthesis of complementary DNA was performed using 1–2 μg total RNA and the High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Quantitative real-time PCR reactions were performed using SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2024Quote: qRT-PCR was performed using the Power SYBR Green 1-step kit and the 7500 Fast Real-Time PCR System (Applied Biosystems). Relative expression was calculated using the comparative Ct method with Actin B (ACTB ...
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bioRxiv - Bioengineering 2024Quote: Cell viability was assessed following shear stress application by incubating each sample in a solution containing 2 µM calcein AM and 4 µM ethidium homodimer-1 in PBS (Live/Dead Viability/Cytotoxicity Kit; Thermo Fisher) for 30 minutes at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were synthesized from 1 µg of total RNAs using the RevertAid H Minus First Strand cDNA Synthesis Kit (Fisher Scientific) with oligo(dT ...
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bioRxiv - Genomics 2024Quote: ... Gene expression was measured using qPCR with reagents from the Power SYBR Green RNA-to-Ct 1-step kit (Applied Biosystems), 50ng RNA per reaction ...
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bioRxiv - Genetics 2024Quote: ... 1 μg was used as template for dsRNA synthesis using the MEGAscript T7 transcription kit (Thermo Fisher Scientific Inc., Waltham, MA). The dsRNA was purified using the Direct-zol RNA miniprep kit (Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted from PANC-1 cells using the PureLinkTM Genomic DNA Mini Kit (Invitrogen, Carlsbad, CA; catalog no. K1820-01).
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bioRxiv - Immunology 2024Quote: Cytokines were quantified from 1 ml right lung homogenate using the ProcartaPlex Mouse 12-Plex Mix&Match kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: cDNAs were generated from 1 μg off RNA isolated using the SuperScript II Reverse Transcriptase kit for RT-qPCR (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: IL-1β ELISA was performed according to manufactures instructions using the Invitrogen IL-1 beta Mouse Uncoated ELISA Kit (Invitrogen, #88701388) with the exception that half-volume ELISA plates were used.
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bioRxiv - Immunology 2023Quote: ... Both assays were performed using the Taqman Fast Virus 1-Step Master Mix RT-qPCR kit (Applied Biosystems Inc., Carlsbad, CA) on an LC96 instrument (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized from either 1 μg (tissue) or 100 ng (organoid) of total RNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qPCR was performed using SYBR™ Green master mix (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Relative gene expression was determined using the TaqMan® RNA-to-CT™ 1-Step Kit (ThermoFisher Scientific, Waltham, MA, USA). Reactions were developed in a LightCycler® 96 (Roche ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 μg total RNA from each stimulated sample using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was generated from 1 µl of diluted RNA sample using SuperScript IV VILO Master Mix Kit with ezDNase enzyme (ThermoFisher, 11766050). Quantitative PCR was performed with TaqMan probes for target genes Hr (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... was performed on 200 ng to 1 µg DNase I-treated RNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative PCRs (qPCRs ...
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bioRxiv - Immunology 2023Quote: ... and 1 × 10^6 were processed using Click-iT EdU Pacific Blue Flow Cytometry Assay Kit (Thermo Fisher Scientific Cat. #C10418). The other 1 × 10^6 were subjected to anti-CD3 crosslinking and pERK staining as described below ...
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bioRxiv - Microbiology 2023Quote: ... followed by HRP-labeled anti-mouse IgG (1:50000 dilution) and quantified with Pierce™ BCA Protein Assay Kit (Life Technologies). GST-PfPP1c and PfPP1c were produced as previously described (Hollin et al. ...
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bioRxiv - Neuroscience 2023Quote: ... RNA concentration was measured using a nanodrop and 1 µg of RNA was converted to cDNA using the RevertAid First Strand cDNA Synthesis Kit (ThermoFisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... The azide group of ADA-DA was labelled (post-fixation) with 5 µg ml−1 Alexa Fluor 488 Alkyne using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen) according to the manufacturer instructions.
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bioRxiv - Physiology 2023Quote: ... 1 μg of RNA was converted to cDNA using the High-Capacity RNA to cDNA kit (Applied Biosystems, Foster City, CA). For qRT-PCR ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... 1 µg of total RNA was used for cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, CA). Quantitative analysis of target gene expression was performed on a 7500 Applied Biosystems real-time PCR system (Foster City ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pooled and dried to near completion under reduced pressure before resuspension in 1% formic acid and fractionation using a Pierce High pH Reversed Phase Peptide Fractionation Kit (ThermoFisher Scientific) with modified elution of 12 sequential fractions (10% ...
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bioRxiv - Cancer Biology 2023Quote: ... human NRP-1 (AD5-17F6; Milteny Biotec) pre-labeled with Alexa Fluor 647 using an Ab labeling kit (Molecular Probes, A20186). Images were acquired with an LSM 710 confocal microscope (Carl Zeiss ...
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bioRxiv - Biophysics 2023Quote: ... The amount of Pi released from actin was measured by absorbance at 360 nm every 20 seconds for 30 minutes to 1 hour using an EnzChek phosphate assay kit (Thermo Fisher) (63 ...
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bioRxiv - Genomics 2023Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Cancer Biology 2023Quote: ... qRT-PCR products were measured using Power SYBR Green RNA-to-Ct 1-step kit (Applied Biosystem, #4389986) and the 7500 Fast Real-Time PCR System (Applied Biosystems). Sequences for gene-specific qRT-PCR primers used are listed in the Supplementary Materials ...
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bioRxiv - Cell Biology 2023Quote: ... PCS-400-030), supplemented with Renal Epithelial Cell Growth Kit (ATCC, PCS-400-040) and 1% antibiotic/ antimycotic solution (Gibco, 15240062) and incubated at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA (1 μg) was reverse-transcribed into complementary cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA (1 μg) was reverse-transcribed in a 20 μL reaction using RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, #K1622). After reverse transcription at 42°C for 60 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Concentration was determined in a ND-1000 spectrometer (Nanodrop) and 1 µg of total RNA was converted to cDNA using the Omniscript Reverse Transcriptase RT Kit (Invitrogen, USA). Quantitative Real-Time PCR (qRT-PCR ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 1 µg of each total RNA was subjected to RT-PCR using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of isolated total RNA was reverse-transcribed to cDNA using the RevertAid First Strand cDNA synthesis kit (Life Technologies). qPCR was performed with 25 ng cDNA template using TaqMan Expression Master Mix (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... A total of 1 μg of RNA was reverse transcribed with random primers to cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). For qRT-PCR reaction ...
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bioRxiv - Physiology 2023Quote: ... A total of 1 μg per sample was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific, Ghent, Belgium) + Tris Buffer HCl pH 7,4 (1:1 ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR was carried out using SYBR Green Pro Taq HS qPRC Kit (Accurate Biology, China) with the QuantStudio 1 system (Applied Biosystems). To normalize total RNA quantity of different samples ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... cDNA were synthetized from 1 mg of total RNA using the Maxima™ Minus cDNA Synthesis Master Mix kit (ThermoFisher, #M1662) according to the manufacturer’s instructions ...