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Citations for Thermo Fisher :
8151 - 8200 of 10000+ citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Cell count was measured on days 1 and 7 using a using Quant-iT™PicoGreen™dsDNA Assay Kit (ThermoFisher Scientific). The scaffolds were washed for 5 minutes in PBS and then frozen at -80⍰C ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 µg total RNA from each sample was reverse-transcribed using RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific™) according to the kit instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was carried out with 1 μg of total RNA using random hexamer primers and the SuperScript IV reverse transcriptase kit (Thermo Fisher Scientific). Quantitative PCR was performed using Kapa SYBR Green on a Lightcycler 480 (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2 viral RNA was detected by reverse transcription and amplification using the TaqMan RNA-to-CT 1-Step Kit (Thermo Fisher Scientific), utilizing the primers and probe sequences for viral RNAs are as described before [76] ...
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bioRxiv - Molecular Biology 2024Quote: ... The hybridization signal from the biotin-labeled probe (listed in Supplementary Table 1) was detected using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... We assessed total RNA sample quality on a 1% agarose gel and quantified the RNA in each sample with a Qubit 2.0 High Sensitivity kit (ThermoFisher Scientific, Waltham, MA). We then pooled developmental stages (embryos and juveniles ...
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bioRxiv - Bioengineering 2024Quote: ... cDNA was synthesized from 1 µg of RNA by following the protocol of the high-capacity cDNA reverse transcription kit (Applied Biosystems; 4368814). Real time PCR was performed using PowerTrackTM SYBR green master mix (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... Capture antibodies (provided in Supplementary Table 1) were biotinylated using the EZ-Link micro Sulfo-NHS-biotinylation kit from (Thermo Fisher Scientific). The biotinylated antibodies were then diluted to 10 μg/mL in a 1 % BSA solution and added to the grids for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... cDNA was synthesized from 1 µg of RNA by following the protocol of the high-capacity cDNA reverse transcription kit (Applied Biosystems; 4368814). Real time PCR was performed using PowerTrackTM SYBR green master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... was expressed in Expi293 cell line (3X106 cells/ml, 200 ml in 1 L flask) using ExpiFectamine™ 293 Transfection Kit (Gibco, A14524). After 48 hours ...
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bioRxiv - Cell Biology 2023Quote: between 0.2·106-1·106 of VS derived cells were reprogrammed using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Thermo Fisher Scientific), a non-integrative cell reprogramming method holding the four Yamanaka factors (OCT4 ...
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bioRxiv - Bioengineering 2024Quote: PCR products were run on a 1% agarose gel and purified using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, cat.: FERK0692). Sanger sequencing of respective PCR samples was performed using the reverse primer ...
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bioRxiv - Neuroscience 2023Quote: ... 1 μg of RNA was used for reverse transcription using the High Capacity RNA-to-cDNA kit (Life Technologies, cat. no. 4387406), and cDNA samples were diluted with nuclease-free water at a 1:2 ratio ...
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bioRxiv - Molecular Biology 2022Quote: Equal amounts of RNA (0.5–1 μg) were reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific #4368813) with the random primers provided ...
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bioRxiv - Immunology 2022Quote: ... the tissue sections were washed twice for 2 minutes in wash buffer and then incubated with a 1:10 dilution of ELF97 phosphatase substrate (E6600, ELF™ immunohistochemistry kit, Invitrogen by Thermo Scientific) for 10-20 minutes ...
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bioRxiv - Genomics 2023Quote: ... The DNA integrity of the pool was controlled on 1% agarose gel and quantified on Nanodrop and Qubit using the Qubit™ dsDNA HS Assay Kit (Invitrogen™).
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bioRxiv - Molecular Biology 2023Quote: ... faf2 and tm6sf2 gene targets (see Table 1 for primers [26] and probes) as described in the manual of the manufacturer (Precision gRNA Synthesis Kit, Thermo Fisher Scientific). A 1:1 solution of gRNA and 500 µg/mL of Cas9 nuclease V3 (Integrated DNA Technology Iowa 52241 ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was tested using the ”LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells” that contained Ethidium Homodimer-1 and Calcein-AM stain (ThermoFisher ; L-3224) dyes ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg of total RNA was reverse transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, Cat 4368814). qPCR was performed in duplicates for each biological replicate in a 20 µl reaction ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed with 1% formaldehyde for 10 min and stained using the Click-IT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... pombe SY78 cells were mixed in an 8:1 ratio for each condition and RNA was isolated using the Ribopure Yeast Kit (Ambion Cat# AM1924). 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066) ...
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bioRxiv - Molecular Biology 2023Quote: ... Up to 1 µg purified DNA was in vitro transcribed overnight at 37°C with the MAXIscript T7 Transcription Kit (Thermo Fisher Scientific). The synthesized sgRNAs were precipitated from the reaction using Ammonium acetate Stop Solution as described for the mMessage mMachine Kit protocol (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... construct was ordered from GeneArt Strings (Supplemental file 1) and cloned into pJET1.2 plasmid using the CloneJET PCR Cloning Kit (Thermo Fisher Scientific, K1231). To remove the DP75 and 3xFLAG-DP75 regions from the uORF constructs ...
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bioRxiv - Genomics 2023Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μg of RNA was then used to reverse transcribe cDNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, 4368813) following ...
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bioRxiv - Genomics 2022Quote: ... One-step reverse transcription and real-time PCR was performed with a Quantstudio 5 using Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) following the manufacturer protocol ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... PCR products were loaded onto a 1% agarose gel to check for the correct size and purified with the GeneJET PCR Purification kit (Thermo Fisher Scientific). The products were then used as template for in vitro dsRNA transcription using the MEGAscript RNAi Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA was dissolved in RNAse-free water and 1 µg estimated using Nanodrop was used to make 20 µl cDNA (Verso cDNA synthesis kit, Thermo Fisher Scientific). cDNA was amplified using the PowerUp SYBR Green master mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... RNA-seq libraries were prepared with 1 ng of total RNA using the Ion AmpliSeq Transcriptome Mouse Gene Expression kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Synthesis of cDNA was performed on 1 µg of DNAse-free RNA using the SuperScript VILO cDNA Synthesis Kit (Invitrogen, Thermo Fisher). Power SYBR Green PCR Master Mix (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The reverse transcription was carried out with 1 μg total RNA using the Maxima Hminus First Strand cDNA Synthesis Kit (Thermo Fisher, K1652). Quantitative PCR (qPCR ...
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bioRxiv - Immunology 2023Quote: ... a Luminex assay using the ProcartaPlex™ Mouse Cytokine & Chemokine Panel 1 36-plex kit (#EPXR360-26092-901, ThermoFisher Scientific, Waltham, MA) was performed according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... 0.5-1 µg of total RNA was isolated from human islets and reverse transcribed using an M-MLV RT kit (Invitrogen, MA, USA). TaqMan primers (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then treated with a 1:1000 dilution of BacMam enhancer solution from a BacMam Enhancer Kit (B10107; Thermo Fisher Scientific) for 1.5 hours ...
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bioRxiv - Microbiology 2023Quote: ... Then each sample was diluted in a 1:10 ratio and measured by the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 μg total RNA was reverse transcribed to generate cDNA using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, K1621). The cDNA was then used as templates and qPCR was performed using the SYBR Green qPCR Master Mix (Solomon Biotech ...
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bioRxiv - Molecular Biology 2023Quote: ... Each RNA sample (1 µg) was subjected to DNase treatment and cDNA synthesis using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of 1 µg RNA were reverse-transcribed with the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA). For relative quantification of mRNA ...
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bioRxiv - Microbiology 2023Quote: ... and JEV RNA was quantified using a Power SYBR green RNA-to-Ct 1-Step kit (Thermo Fisher Scientific, Waltham, MA, USA) and the AriaMx Real-Time PCR system (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... followed by click-chemistry using EdC-labeled HSV-1 DNA and the Click-iT EdU Alexa Flour 555 Imaging Kit (ThermoFisher Scientific, C10638) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNase treatment was given to the extracted RNA for 15 mins at RT using DNase-1 kit (cat no.18068-015, Gibco/Thermofisher scientific) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... SYBR Green assay: PCR reactions were prepared using the Power SYBR Green RNA-to-CT 1 step kit (Applied Biosystems, Cat# 4391112). Reactions contained a 2× Power SYBR Green RT-PCR Mix ...
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bioRxiv - Cell Biology 2024Quote: ... ∼1 μg RNA of each sample was used to generate cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, # 4368813) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 1 μg of RNA using the H Minus cDNA first strand synthesis kit (Thermo Scientific, Cat. No. K1652). Relative mRNA expression was determined by quantitative PCR using the PowerUpTM SYBR TM Green Master Mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of RNA was reverse transcribed into complementary DNA (cDNA) utilizing the high-capacity RNA to cDNA kit (Life Technologies #4368813). For qPCR ...
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bioRxiv - Biochemistry 2024Quote: ... Complementary DNA (cDNA) was reverse-transcribed from 1 µg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The constructs were used for in vitro transcription and translation using the 1-Step Human High-Yield Mini IVT Kit (Thermo Fisher; 88891) according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2024Quote: ... One-step reverse transcription and real-time PCR was performed with a Quantstudio 5 using Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) following the manufacturer protocol ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from 1 µg of total RNA using RevertAid First Strand cDNA Synthesis Kit and oligo (dT)18 primers (Thermo Fisher Scientific) and diluted 2 times prior to use ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA synthesis was performed using 1 µg of DNA-free RNA sample with RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...