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Citations for Thermo Fisher :
7801 - 7850 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the cells were seeded at low and high density in 6-or 12-well plates coated with 0,2% type 1 Collagen (Gibco) and grown in PM for 24 hours ...
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bioRxiv - Genetics 2021Quote: ... and Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) plus GlutaMax (Thermo Fisher), supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... NALM-6 cells were cultured in RPMI media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco). MCF7 cells were cultured in DMEM media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... Intact 12 day-old seedlings were transferred in 8 mL of 1% liquid MS media (4.4 g/L MS salts mixture, 10 g/L D-sucrose) in 6 well-plates (Nunc) and allowed to acclimate for 48 hours with mild agitation (130 rpm).
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bioRxiv - Microbiology 2021Quote: ... MDCK cells were grown to 90-95% confluence in 6-well tissue culture plates in DMEM (ThermoFisher Scientific/Gibco) containing 10% FBS (ThermoFisher Scientific/Gibco ...
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bioRxiv - Microbiology 2020Quote: 4 μl of purified spores (6×107 spores/ml) were mixed with 40 μl of NucBlue (Invitrogen, catalog #R37605) and incubated at 25°C for 40 min ...
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bioRxiv - Microbiology 2021Quote: Bone marrow derived macrophages (BMDMs) were generated from 6-8 week old BALB/c mice in RPMI-1640 (Gibco) containing 10% foetal calf serum ...
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bioRxiv - Molecular Biology 2021Quote: ... 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 6% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco, 15240062). T cell lines were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Microbiology 2021Quote: ... were used to amplify cellular RNA and viral RNA by QuantStudio 6 Flex Real-Time PCR Systems (Applied Biosystems). The relative expression levels of SARS-CoV-2 N gene was calculated using the standard curve method and normalized to 18S ribosomal RNA as an internal control.
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bioRxiv - Immunology 2020Quote: 250 μL K92 peptide at 1 mg/mL was reduced with 6 μL of 0.5 M Dithiothreitol (Thermo Fisher) for 40 minutes at 37 °C and alkylated with addition of 18 μL of 2-Iodoacetamide (Thermo Fisher) ...
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bioRxiv - Genetics 2021Quote: ... The samples were either frozen (n = 6) or stored in the RNAlater reagent (Thermo Fisher Scientific, Waltham, MA, USA) (n = 4).
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bioRxiv - Genomics 2020Quote: ... standards (3 x 10μl) and samples (6 x ∼18μl) were loaded on a 4-12% Bis-Tris Nu-PAGE (Life Technologies) gel for electrophoresis ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Immunology 2022Quote: ... The presence of SARS-CoV-2 RNA in these samples was evaluated by one-step RT-qPCR in a final volume of 12.5LμL per reaction in 384-wells PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Molecular Biology 2022Quote: HeLa cells were grown to 80% confluency in 6-well plates and transfected with 300 ng pcDNA3.1-FLAG-RBM39 constructs using Lipofectamine2000 (Invitrogen). On the following day ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa RAD54KO cells were transfected with 3μg of RAD54-GFP or eGFP plasmid per well in a 6-well plate using Lipofectamine LTX PLUS (Invitrogen). Stable cell lines were generated by selecting with neomycin (800μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.5×105 4T1 cells were co-cultured in 6 wells plates using Transwell plates (0.4 μm, Nunc, ThermoFisher Scientific) with Gr1+CD11b+ sorted cells (top ...
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bioRxiv - Cancer Biology 2022Quote: ... 1.5×105 4T1 cells were co-cultured in 6 wells plates using Transwell plates (0.4 μm, Nunc, ThermoFisher Scientific) with Gr1+CD11b+ sorted cells (top ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were incubated at 37°C for 30 min and loaded onto 6% DNA retardation gels (Thermo Fisher Scientific). DNA was detected by staining the gel with GelRed (VWR) ...
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bioRxiv - Cancer Biology 2022Quote: Total cellular RNA was extracted post-treatment in HT-29 cells (1.5×105/well) plated in 6-well plates (Nunc) at indicated time-points ...
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bioRxiv - Biophysics 2022Quote: ... thoracic and lumbar DRGs were harvested from 4 to 6 week old MrprD-GFP mice and transferred to Hank’s buffered saline solution (HBSS) (Invitrogen). Ganglia were treated with collagenase (2 mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: Modified hESCs were cultured and propagated on Matrigel-coated 6-well culture dishes in Stem Flex medium (Thermo Scientific) supplemented with 100 U/ml Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSC at day 6-7 after passaging were treated with a 1:1 mixture of TrypLE Select (Life Technologies) and 0.5 mM EDTA/phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... 60 °C for 1 min) X 49 cycles on the QuantStudio 6 flex Real-time PCR system (Applied Biosystems), using the TaqMan RNA-to-CT 1-step kit (Applied Biosystems).
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bioRxiv - Microbiology 2022Quote: ... or 6 µL of H2O (negative control) and run in a StepOne™ Real- Time PCR System (ThermoFisher scientific) with the following program ...
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bioRxiv - Molecular Biology 2022Quote: ... 6 replicates of each condition were treated with DMSO or 100 nM dTAG13 (Tocris Bioscience, Fisher Scientific 66-055) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... adult WT C57BL/6J mouse testes were dissected and incubated at 37 °C for 6 h in either Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12, Thermo Scientific) or compound 5 (100 μM ...
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bioRxiv - Neuroscience 2022Quote: ... of 0.5% low-melting agarose (FMC) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... Slides with 6 μm sections were mounted in ProLong Glass Antifade Mountant (Thermo Fisher Scientific, Sugar Land, TX, USA) and those with 35 μm sections in SlowFade Glass Soft-set Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were allowed to rest in fresh slicing solution at 4 °C for 30-45 minutes before being mounted on individual semiporous membrane inserts (2 slices per insert) placed in 6 well plates with 1.1mL of culture media [50% Minimal Essential Medium (MEM; Gibco), 25% heat-inactivated horse serum (Gibco) ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Quantitative RT– PCR was performed using the QuantStudio-6 and -7 Flex Real-Time PCR Systems (Thermo Fisher Scientific) with SYBR® Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Plant Biology 2023Quote: ... was employed for reporting successful amplification using a QuantStudio 6 Pro real-time PCR system (Applied Biosystems, Waltham MA) with specific primer pairs for each target NbBBL gene ...
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bioRxiv - Immunology 2022Quote: ... qRT-PCR was performed by a QuantStudio 6 Flex real-time PCR detection system (Applied Biosystems, Foster City, CA) with SYBR Premix Ex TaqTM II (Tli RNaseH Plus ...
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bioRxiv - Microbiology 2022Quote: Vero cells in 6-well plates were transfected with 1.5 μg/well pQF356 using Lipofectamine 2000 (Invitrogen, Carlsbad, CA). Then the cells were seeded in 100 mm plates in medium containing 800 μg/mL G418 to allow colonies to develop for two to three weeks ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Bioengineering 2022Quote: ... was buffered to a final concentration of 6 mg/mL at pH 7.4 with 250,000 cells/mL using 10X phosphate buffered saline (PBS) (Thermo Fisher) with phenol red ...
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bioRxiv - Developmental Biology 2022Quote: Lungs from experimental C57BL/6 mice were inflated with an enzyme mix consisting of the following: Leibovitz media (Invitrogen) 2mg/mL of collagenase type 1 and 2mg/mL of elastase (Worthington Biochemical) ...
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bioRxiv - Neuroscience 2024Quote: ... and CHIR 99021 (Cat# 72052) in sterile non-tissue culture treated 6-well plates (Thermo Fisher Scientific, Cat# 150239). NSCs were passaged using either accutase (Stem cell technologies ...
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bioRxiv - Pathology 2024Quote: ... Cells were subcultured at a 1:3 to 1:6 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Microbiology 2024Quote: ... The optical density at 600 nm (OD600nm) was measured with a Genesys 6 spectrophotometer (Thermo Fisher Scientific, United States). The preculture was next diluted into a baffled shake flask (250 ml ...
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bioRxiv - Plant Biology 2024Quote: ... 8 and 6 hours were then transferred to MS medium containing zeocin with Actinomycin D (Thermo Fisher, J60148.LB0) or zeocin with DMSO (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Cells were collected from one well of a 6-well plate using 700 µL of TRIzol Reagent (Life Technologies) and RNA was extracted using Direct-zol RNA Miniprep (Zymo Research) ...
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bioRxiv - Genetics 2024Quote: ... 2 x 105 cells were seeded in 6-well plates and grown overnight in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: Hepa1-6 (ATCC CRL-1830) cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) plus GlutaMAX (Thermo Fisher Scientific), supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2024Quote: ... and 6 µg of psPAX2 plasmids into 293T cells grown in a 10-cm dish using Lipofectamine 2000 (Invitrogen). The cell culture supernatant was collected 48 h after transfection and used to transduce parental HeLa cells in the presence of polybrene (8 µg/mL) ...
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bioRxiv - Immunology 2023Quote: ... and the reactions were measured using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). The relative fold change in gene expression compared to the 471_IgG1H_28z CAR-T cells is represented in the graph.
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bioRxiv - Immunology 2023Quote: ... and the reactions were measured using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific). The relative fold change in gene expression compared to the 471_IgG1H_28z CAR-T cells is represented in the graph.