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Citations for Thermo Fisher :
7851 - 7900 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 3 µg of each plasmid was mixed with 6 µl of Plus reagent and 7.5 µl of Lipofectamine LTX (Invitrogen) reagent ...
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bioRxiv - Microbiology 2024Quote: ... and 6 µg of psPAX2 plasmids into 293T cells grown in a 10-cm dish using Lipofectamine 2000 (Invitrogen). The cell culture supernatant was collected 48 h after transfection and used to transduce parental HeLa cells in the presence of polybrene (8 µg/mL) ...
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bioRxiv - Immunology 2023Quote: ... The paraffin blocks were sliced into 5 to 6 μm sections using a Microm HM 325 microtome (Thermo Scientific), and the sections were stained with Hematoxylin and Eosin (H&E) ...
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bioRxiv - Cell Biology 2023Quote: ... coverslips with the gel stuck to it were transferred to a 6-well plate with 1 ml of digestion solution (0.5 % Triton X100, 1 mM EDTA, pH 8 (AM9260G, Ambion), 50 mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/mL)-coated 6-well plates in hECSR medium (Human Endothelial SFM, Thermo Fisher, cat# 11111-044; B-27 Supplement (50×), Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... were seeded in a 6-well plate at a cell density of 200,000/cm2 in Dulbecco’s Modified Eagle Media (DMEM, Thermo Fisher) supplemented with 15% fetal bovine serum (Gibco ...
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bioRxiv - Immunology 2023Quote: ... and rested for ∼6 hours in R15 (RPMI 1640 medium supplemented with 15% FBS, 1% antibiotic-antimycotic [Thermo Fisher Scientific] ...
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bioRxiv - Immunology 2022Quote: ... All samples were prepared in triplicate and run on a QuantStudio 6/7 Flex Real-Time PCR System (ThermoFisher). Primer sequences for designated HHV-6 marker (U31 ...
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bioRxiv - Microbiology 2023Quote: ... live/dead staining was carried out using 6 μM syto 9 and 30 μM propidium iodide (BacLight, Molecular Probes). Biofilms were imaged using a Zeiss LSM 700 compact confocal laser scanning microscope using the 40x objective and appropriate fluorescence settings (syto 9=488 nm laser ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa were prepared in 6-well plates 24 hours before the transfection with 4.5.105 cells/well in RPMI medium (Gibco-Invitrogen) supplemented with 10% fetal calf serum (Gibco-Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... and plaque-derived sEVs (n=6) were mixed with NuPAGE lithium dodecyl sulfate sample buffer (NP0007, Thermo Fisher Scientific) at a 4:1 ratio and NuPAGE sample reducing agent (NP0009 ...
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bioRxiv - Cell Biology 2023Quote: Cultured N2A control and Fmr1 CRISPR/Cas9 KO cells were seeded in 6-well plates overnight and then transfected using 7.5 µl of Lipofectamine 3000 (Invitrogen) and 5 µl of P3000 with the 1 µg of pFlareA-Mapt exon4 splicing reporter ...
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bioRxiv - Cell Biology 2023Quote: ... The cells from each embryo were then plated onto a 6-cm culture dish coated with 0.1% gelatin and cultured in DMEM (Hyclone) supplemented with 10% FBS (GIBCO), 1% GlutaMAX (GIBCO) ...
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bioRxiv - Cancer Biology 2023Quote: ... normalized to the expression of GAPDH on a QuantStudio 6 and 7 Pro real-time PCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-23,36-39 (Supplementary Methods Table 2 ...
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-59 (Supplementary Methods Table 2 ...
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bioRxiv - Bioengineering 2023Quote: ... Following 6 days of expansion the filtered cell culture supernatant was applied to a 5ml Ni-NTA column (ThermoFisher) prewashed with 100ml washing solution (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Thermo Scientific’s SYBR green Real-time PCR master mix was used to perform qRT-PCR (Quantstudio 6, ThermoFisher Scientific) in triplicates to determine the fusion gene expression levels using PRCC-TFE3 fusion gene primers (FP:5’-CCAAGCCAAAGAAGAGGAAA-3’ and RP:5’- CGAGTGTGGTGGACAGGTACT-3’).
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bioRxiv - Molecular Biology 2023Quote: ... TNF-α and IL-6 in cell culture supernatants were assayed using standard ELISA kits (Invitrogen or R&D) in accordance with the protocol set out by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant containing secreted pro-HlyA protein was collected by centrifugation (Thermo Scientific, Sorvall RC 6+, SLA-3000 (Rotor), 10,000 × g for 30 min at 4°C ...
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bioRxiv - Genomics 2023Quote: ... and 2µL of primers at 3µM in a total reaction volume of 10µL on qPCR was realized on a QuantStudio 6 Flex Real-Time PCR system (ThermoFisher). Each sample was tested in duplicate ...
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bioRxiv - Bioengineering 2023Quote: ... and liquid agarose (2.5mL) was used to cover the bottom of a high-performance #1.5 6-well plate (Fisher Scientific). This process was repeated for all five wells ...
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bioRxiv - Pathology 2023Quote: Primary human lung fibroblasts (n = 6 for both control and IPF, all at passage 5) were harvested using 0.25% Trypsin-EDTA (Gibco) and centrifuged at 500 x g for 5 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293T cells were seeded in 6-well plates and transfected with 1µg of plasmid DNA per well using Lipofectamine 2000 (Invitrogen) as transfecting reagent ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Gene expression was determined by quantitative real-time PCR using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative gene expression was determined using ΔΔCT method ...
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bioRxiv - Neuroscience 2023Quote: ... Organ of Corti explants were isolated from C57BL/6 mice at postnatal day 4 in cold Leibovitz’s L-15 medium (cat. # 21083027, Gibco) and held in place by two glass fibers glued to the bottom of a Petri dish ...
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bioRxiv - Genetics 2023Quote: 3 independent total RNA extractions from 30 ovaries from 3-6-day-old RevI-H2i2 flies using Trizol (Invitrogen) were performed ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were seeded at a concentration of 150,000 cells per well of a Matrigel-coated 6-well plate in E8 medium (ThermoFisher) containing Rock-inhibitor (10 µM ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293A cells were initially plated at 750k cells/well in 6-well plates in Dulbecco’s modified eagle media (DMEM) supplemented with 10% fetal bovine serum (FBS) and GlutaMax (Gibco) with 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and 6 µg of PEI were diluted in 0.5 ml of Opti-MEM™ Reduced serum medium (Gibco™) per single well ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were assembled for 20 min at room temperature and separated on 6% DNA retardation gels (Thermo Fisher scientific) at 200 V for 1–2 h ...
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bioRxiv - Cell Biology 2022Quote: ... 1 µg FLAG-AGAL construct and 4.5 µg pOG44 was transfected into HFTs seeded onto a 6-well plate using Lipofectamine 2000 (Invitrogen). After 48 hours the transfected cells were selected with hygromycin (200 µg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... 8 days after transfection antibiotic selection was performed using 6 µg mL-1 blasticidin S (Thermo Fisher Scientific, A1113903) to identify cells which had undergone productive editing ...
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bioRxiv - Cell Biology 2023Quote: ... supplied with the primers for ICP27 and β-actin on a Quant Studio 6 Flex qPCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2022Quote: ... and an oxygen-scavenger system (60□mg ml−1 glucose oxidase, 6□mg□ml−1 β-ME (Gibco, 21985023)) were used for all STORM imaging experiments.
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bioRxiv - Developmental Biology 2023Quote: ... Images were collected manually or automatically with 4-6 µs dwell time using Maps 3.11 software (Thermo Fisher Scientific) (Burel et al. ...
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SHARPIN enhances ferroptosis in synovial sarcoma cells via NF-κB- and PRMT5-mediated PGC1α reductionbioRxiv - Cancer Biology 2023Quote: Yamato cell lines were seeded into 6-well plates and incubated with 5 µM BODIPY 581/591 C11 (Invitrogen) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and SEC using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The RT-qPCR was performed using the Promega® 2x Master Mix and the QuantStudio™ 6 (Applied Biosystems) for 40 cycles ...
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bioRxiv - Pathology 2023Quote: ... Cells were spun down at 350g for 6 minutes and were re-suspended with 4mL of 0.05% Trypsin (ThermoFisher) and incubated at 37°C for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Microbiology 2024Quote: ... The optical density at 600 nm (OD600nm) was measured with a Genesys 6 spectrophotometer (Thermo Fisher Scientific, United States). The preculture was next diluted into a baffled shake flask (250 ml ...
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bioRxiv - Genetics 2024Quote: ... 2 x 105 cells were seeded in 6-well plates and grown overnight in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco #11995073) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: Cells were collected from one well of a 6-well plate using 700 µL of TRIzol Reagent (Life Technologies) and RNA was extracted using Direct-zol RNA Miniprep (Zymo Research) ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: Total RNA from mating wild-type and EMA2 KO cells were isolated at 6 hpm using TRIzol reagent (Invitrogen). The RNA was treated with Turbo DNase (Ambion ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6) at 40°C with vigorous shaking at 1400rpm for 30 minutes using a thermal mixer (Thermo Scientific). The eluted DNA and previous input samples were then amplified using the PCR Primer Mix 3 for Illumina and the VAHTS HiFi Amplification Mix 2 (ND607 ...