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Citations for Thermo Fisher :
7651 - 7700 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation ...
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bioRxiv - Physiology 2023Quote: ... Sense and antisense sequences targeting daf-2 or aak-2 were amplified from N2 lysates and subcloned into donor vectors using Gateway Cloning Technology (Life Technologies). The final plasmids for the sense and antisense expression of daf-2 or aak-2 under the str-3 promoter were generated using Gateway Cloning Technology (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The culture dish was coated with 0.2% gelatin and the cells were cultured in endothelial growth medium (EGM)-2 complete media (#CC-3162, Lonza Clonetics, Fisher scientific) with EGM-2 SingleQuots supplement kit (CC-4176 ...
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bioRxiv - Microbiology 2023Quote: ... after which metal beads with DNA absorbed on their silica surface were recovered using the DynaMag™-2 Magnet tube holder (12321D-DynaMag-2 – Invitrogen) and washed twice with AMMLAV/E buffer (10 mM Tris pH 8 ...
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bioRxiv - Microbiology 2023Quote: 3 μL of 3 mg/mL purified Umb1 particle samples were loaded onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a Vitrobot Mark IV (ThermoFisher Scientific) with a blot force of 0 and 6 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Microbiology 2023Quote: ... The resulting peptic peptides were collected on a trap column cartridge (2 mm x 2 cm) that was filled with POROS 20 R2 reversed-phase resin (Thermo Scientific) and kept at 0.5 °C for 3 min ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Peptide mixtures were separated at a 400 nl/min flow rate using a 35 min ACN gradient in 0.5% acetic acid (5–38% ACN gradient for 23 min followed by 38-60% ACN gradient for 3 min and 60-95% ACN gradient for 2 min plus another 3 min at 95% CAN prior to 95%-5% gradient for 2 min and another 2 min at 5% ACN) and detected on an Q Exactive HF mass spectrometer (Thermo Scientific). Dynamic exclusion was enabled for 20 sec and singly charged species ...
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bioRxiv - Microbiology 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids72 prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5.5 sec blot time at 100% humidity and 22°C ...
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bioRxiv - Cell Biology 2024Quote: ... 11.2 and 20.2 µg/mL) concentrations as well as the following additional small molecule inhibitors were collected: puromycin (2 µg/mL; ThermoFisher, A1113803), rapamycin (9.1 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... an IonPac AS24 analytical column and IonPac AG24 guard column (2 x 250 mm and 2 x 50 mm, Thermo Scientific) were connected to a Dionex Ultimate 3000 HPLC system (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and 10 μg/mL Penicilin-Streptomicin) supplemented with 2 mM L-Glutamine (ref.07100, Stemcell) and 10 mM 2-Mercaptoethanol (ref.11528926, Thermo Fisher). The medium was partially changed every other day extremely slowly to prevent disturbing the EB formation ...
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bioRxiv - Immunology 2024Quote: ... 2.7 μl of the mixture was pipetted to Quantifoil R 2/2 gold grids with vitrobot Mark IV (Thermo Fisher Scientific) using parameters set to 4 °C with 95% humidity and vitrified with no wait time ...
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bioRxiv - Biochemistry 2021Quote: ... 1 × 106 HEK293T cells were plated in a 6-well dish with 1.5 mL of DMEM growth media (Gibco) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded at 50% confluence in 6-well plates containing round cover glasses (12CIR-1D; Thermo Fisher Scientific). After treatment ...
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bioRxiv - Microbiology 2020Quote: ... 0.02% NaN3 and applied to plasma-cleaned CF-400 1.2/1.3 grids before being blotted for 6 seconds in a Vitrobot Mark IV (Thermo Fisher) and plunge frozen into liquid ethane ...
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bioRxiv - Molecular Biology 2020Quote: ... snap-cooled and separated by capillary electrophoresis (CE) at 12kV for 25min using a POP-6 polymer (Thermo Scientific). “One-pot” U-insertion/U-deletion reactions were assembled by annealing of the U-insertion and U-deletion pre-mRNA/gRNA-hybrid RNAs in separate reaction vials ...
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bioRxiv - Cell Biology 2020Quote: ... cells seeded in a 6-well format (0.3×106 cells/2ml/well) were switched to a galactose medium (glucose-free DMEM (Gibco) supplemented with 10 mM D-galactose (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... Gene expression was analyzed by real-time qPCR using QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) or Biomark microfluidic system using a 48.48 Dynamic Array IFC for gene expression (Fluidigm ...
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bioRxiv - Cell Biology 2020Quote: ... ovaries from randomly cycling female mice (FVB/N, 6 weeks old) were collected and incubated in 0.25% Trypsin/PBS (Invitrogen) (37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... coated 6-well plates or glass coverslips (for smRNA FISH purposes) in Essential 8 Flex medium (Thermo Fisher Scientific) with E8 supplement (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... 2020) and reactions were carried out using a QuantStudio 6 and 7 Flex Real-Time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were incubated in a 10 mM sodium citrate buffer with 0.05 % Tween 20 (pH of 6) (Fisher Scientific) for 15 minutes at 99□ prior to fixation ...
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bioRxiv - Molecular Biology 2021Quote: For siRNA transfection experiments: HEK293 cells were grown in 6-well plates and RNAimax transfection reagent (Invitrogen #13778-150) was used following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: Drosophila S2 cells from the Drosophila Genomics Resource Center (DGRC - stock#6) were cultured in Schneider’s medium (Thermo Scientific) supplemented with 10% Heat-Inactivated Fetal Bovine Serum (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: One hundred and fifty tergal gland segments (Segment 7) and control segments (Segment 6) of Dalotia males were dissected in EBSS (Earle’s Balanced Salt Solution; ThermoFisher) and transferred to into 150 µl ice-cold Schneider’s Drosophila medium and fetal bovine serum (SDM+FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Frozen sections were cut (6-10 μm) and spotted onto positively-charged slides (Superfrost Plus slides-Thermo Fisher Scientific) and stored at -20°C prior to use ...
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bioRxiv - Cell Biology 2021Quote: ... Wild type and Ex6KO cell lines were plated on a 6-well plastic plate (Nunclon Delta Surface,ThermoFisher Scientific). When confluent ...
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bioRxiv - Molecular Biology 2021Quote: ... Datasets were collected on an Applied Biosystems™ QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific). The cycling conditions were as follows ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 ng of end-labeled pNG1175 DNA was mixed with 1 µL streptavidin-functionalized paramagnetic beads (M-270 Dynabeads, diluted 1:6 in 1X PBS with 0.1% BSA; Invitrogen) in 10 µL PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA loading buffer was added and 10 mL of the reactions were electrophoresed on 6% DNA Retardation gels (Invitrogen) at 130 V for 60 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Vero-6 cells (ATCC CRL-1586) were maintained in DMEM medium (Gibco, 4.5 g/L glucose, w/o pyruvate) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Immunology 2021Quote: ... Targeted qPCR was performed using the QuantStudio 6 Real-Time PCR system using the following Taqman Primers (Applied Biosystems): BCL6 (Hs00153368_m1) ...
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bioRxiv - Molecular Biology 2021Quote: ... ESCs were transfected in a single well of a 6-well plate with 0.5μg Cas9 guide plasmid and 2μg targeting construct plasmid using Lipofectamine 3000 (Thermo Fisher Scientific) according to manufacturer’s guidelines ...
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bioRxiv - Zoology 2020Quote: Xela DS2 (passage 74) and Xela VS2 (passage 75) cells were seeded in 6-well plates (BioLite, Thermo Scientific) at cell densities of 300,000 cells/well in 1 mL of complete medium and allowed to adhere overnight at 26°C ...
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bioRxiv - Developmental Biology 2019Quote: HEK cells were grown in 6 well plates and transfected with 4µg of each DNA with lipofectamine 2000 (Invitrogen) for 6hrs according to manufacturers instructions.
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bioRxiv - Microbiology 2019Quote: 293T cells in 6-well tissue culture plates were transfected using Lipofectamine2000 according to manufacturer’s instruction (Thermo Fisher Scientific). For scaleup transient transfection of 293S GnTI- cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 6-12 µg anti-HA antibodies (i.e., 12CA5, University of Chicago Monoclonal Antibody Facility) or 6 µg anti-V5 antibodies (R960-25, Invitrogen) per reaction were first conjugated to protein A sepharose beads in a 50% slurry that was first washed in IPP150 ...
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bioRxiv - Cell Biology 2019Quote: ... and oligonucleotides targeting genes of interest (IDT) in triplicate and run on QuantStudio 6 Flex (Applied Biosystems Cat# 4485694) using protocols according to the mastermix manufacturer’s specifications ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were run on QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, part of Life Technologies Corporation, CA) according to the manufacturer’s recommendations using a melt protocol in 0.05-degree/sec increments from 25°C to 95 °C ...
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bioRxiv - Genetics 2019Quote: ... 2.4×104 cells were seeded per well of a 6-well cell culture plate containing 4ml of DMEM (Gibco) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Sigma#S3139 and Sigma#S9390, 300 mM KCl, Sigma#P9333, 5 mM MgCl2 × 6 H2O, Sigma#M2670, 0.001% Brij35 ThermoFisher, #28316 ...
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bioRxiv - Genetics 2019Quote: ... about 6 males and 10 female flies were flipped onto a new flask (Fisher Scientific, Cat. No.:AS-355) of food and allowed to mate at 25°C and lay eggs ...
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bioRxiv - Molecular Biology 2019Quote: ... or Tg(EF1α:JPXE1-E3) plasmids per well of a 6-well plate according to the Lipofectamine® 2000 (Invitrogen) manufacturer’s instructions while still in the stem cell state ...
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bioRxiv - Molecular Biology 2019Quote: ... or Tg(EF1α:JPXE1-E3) plasmids per well of a 6-well plate according to the Lipofectamine® 2000 (Invitrogen) manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... The cells were then seeded onto Matrigel-coated 6-well plates containing Essential 8™ Medium (ThermoFisher #A15169-01) with added Y-27632 for recovery following nucleofection ...
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bioRxiv - Cell Biology 2019Quote: ... dsRNA for knocking-down Par-6 or aPKC was synthesized with MEGAscript T7 Transcription Kit (Ambion, Thermo Fisher Scientific) according to the manufacurer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... dsRNA for knocking-down Par-6 or aPKC was synthesized with MEGAscript T7 Transcription Kit (Ambion, Thermo Fisher Scientific) according to the manufacurer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... 100×103 cells / well cultured in 6 well plates were transfected with 0.8 μg each plasmid DNA using Lipofectamin2000 following the manufacturer’s protocol (ThermoFisher Scientific) and analysed 48 hours later.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...