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Citations for Thermo Fisher :
7901 - 7950 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Organ of Corti explants were isolated from C57BL/6 mice at postnatal day 4 in cold Leibovitz’s L-15 medium (cat. # 21083027, Gibco) and held in place by two glass fibers glued to the bottom of a Petri dish ...
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bioRxiv - Cell Biology 2023Quote: Interleukin-6 levels in supernatants were determined using commercially available human IL-6 ELISA kits according to the manufacturer’s protocol (Invitrogen). A detailed description of the procedure are reported in Supporting methods ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6) at 40°C with vigorous shaking at 1400rpm for 30 minutes using a thermal mixer (Thermo Scientific). The eluted DNA and previous input samples were then amplified using the PCR Primer Mix 3 for Illumina and the VAHTS HiFi Amplification Mix 2 (ND607 ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted from samples containing 6 OD600 units of mid-log phase cells using Trizol (Invitrogen #15596026) and chloroform ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR (qPCR) was conducted on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, CA, USA). Relative mRNA levels were determined using the Comparative Ct (△△CT ...
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bioRxiv - Cell Biology 2024Quote: ... coverslips with the gel attached were transferred to a well of a 6-well plate with 1 ml of digestion solution (0.5% Triton X100, 1 mM EDTA pH 8 (AM9260G, Ambion), 50 mM Tris pH 8 (AM9855G ...
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bioRxiv - Cell Biology 2024Quote: ... An overnight incubation with specific antibodies (P16, IL-6 or phospho-H2AX (S139) and cTNT (Invitrogen, #MA-5-12960)) was followed by 2 hours of incubation with secondary antibody conjugated with Alexa Fluor 488 ...
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bioRxiv - Plant Biology 2024Quote: ... equal amounts of RNA were prepared in equal volumes (6 µL) using UltraPure DNase/RNase-free distilled water (Invitrogen). RNA samples were denatured at 95 °C for 3 min and placed on ice immediately to prevent the formation of secondary structures ...
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bioRxiv - Cancer Biology 2024Quote: ... typically 1.5 μg of plasmid was reverse transfected into 300,000 293T cells in a well of a 6-well plate using OptiMEM (Gibco) and FuGENE 6 (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a 6× His-tag was cloned into the BamHI and XbaI sites of the pcDNA 3.1 vector (#V79020, Invitrogen) containing the vascular endothelial growth factor receptor 1 (VEGFR ...
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bioRxiv - Microbiology 2024Quote: ... sg.ORF7a) and genomic RNA (NSP3 region; g.NSP3) (90) on a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). Relative mRNA expression was normalized to the levels of GAPDH and expressed relative to the values for control cells using the ΔΔCt method.
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bioRxiv - Molecular Biology 2024Quote: WT and TRBP−/− HeLa cells were plated at 4 x 106 cells / dish into a 9-cm dish and transfected with 6 µg/dish poly(I:C) using Lipofectamine 2000 (Invitrogen). At 0 ...
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bioRxiv - Molecular Biology 2024Quote: ... HEPA1-6 were lysed in RIPA buffer and loaded on a 4-15% gradient polyacrylamide gel (Thermo Fisher, NP0335). Protein transfer was carried out using the iBlot system (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... a 6-well was coated with 1 mL of a 1:200 solution of either Geltrex (Gibco™ A1413202) or Cultrex (Bio-Techne 3445-005-01 ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was incubated for 15 minutes at room temperature and then separated on a 6% DNA retardation gel in 0.5X TBE running buffer (ThermoFisher). The gel was imaged with a SapphireTM multimode imager in the Keck Biophysics facility at Northwestern University.
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bioRxiv - Physiology 2024Quote: ... Cells were grown overnight in glass slides and loaded with 10-6 mol.L-1 4,5-diaminofluorescein-diacetate (DAF-2DA; Molecular Probes) for 30 minutes ...
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bioRxiv - Pathology 2024Quote: ... IRB number: VGHUST105-G7-6-1) were cultivated in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... 2ml serum free DMEM or 2) 2 mL Cell Mask Orange (CMO: 2.5 µg/mL in serum free DMEM) (Thermofisher Scientific cat C10045) and both were incubated at 37° C for 10 minutes.
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bioRxiv - Neuroscience 2020Quote: ... Primary human dermal fibroblasts were collected from VWMD patients (2) or control family members (2) and maintained in DMEM/F12 (Life Technologies, 21331020), 10% FBS (Bovogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... Supernatant was harvested at the indicated timepoints and IL-2 levels in the supernatant were measured via IL-2 Human Instant ELISA kit (Thermo Fisher #BMS221INST). T-cell proliferation was also measured at the indicated timepoints using a BD FACSymphony Fortessa X-50.
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bioRxiv - Plant Biology 2020Quote: ... cinerea germlings exposed to different concentrations NCR044.1 was measured every 30 min for 2 h using the ROS indicator dye 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA, Invitrogen, Carlsbad, CA). ROS levels were quantified by measuring the fluorescence in a SpectraMax® M3 spectrophotometer microplate reader (exc ...
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bioRxiv - Cell Biology 2021Quote: ... cells were pulsed with 10µM 5-Ethynyl-2’deoxyuridine (EdU) for 2 hours following manufacturer’s instructions (Click-iT® EdU Alexa Fluor® Imaging Kit, Life Technologies). Cells were subsequently fixed and stained with Hoechst 33342 ...
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bioRxiv - Molecular Biology 2022Quote: 3 μl of purified ribosome sample was applied to Quantifoil 300 mesh R2/2 grids with an ultrathin 2 nm carbon layer and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) at 15 °C ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
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bioRxiv - Biophysics 2022Quote: ... The sample was diluted to 0.5 mg/ml and directly applied to freshly glow-discharged 2/2 Cu Quantifoil grids in a Vitrobot Mark IV (Thermo Fisher Scientific) at 4° C and 100% humidity ...
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bioRxiv - Microbiology 2020Quote: ... Tryptic peptides (2 μg) were loaded onto a C18 trap (75 um x 2 cm; Acclaim PepMap 100, P/N 164946; Thermo Fisher Scientific) at a flow rate of 2 ul/min of solvent A (0.1% FA and 3% acetonitrile in LC-MS grade water) ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...
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bioRxiv - Neuroscience 2020Quote: ... sequences were amplified from plasmids derived from ser-2(2)p::FRT::YFP,andclonedupstreamof FRT::STOP::FRT::GCaMP6s to generate WY016 using Gateway (Thermo Fisher Scientific). To generate PSAB1206 ...
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bioRxiv - Cancer Biology 2022Quote: ... was diluted in T cell medium (RPMI 1640 + 10%FBS + L-glutamine (2 mM) + penicillin (100 U/ml + streptomycin (100 μg/ml) + 55 μM 2-Mercaptoethanol (Thermo Fisher, #21985023)) ...
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bioRxiv - Molecular Biology 2019Quote: Digested peptides were dissolved in 40 μL of 0.1% formic acid containing 2% (v/v) acetonitrile and 2 μL were injected into an Easy-nLC 1000 system (Thermo Fisher Scientific). Peptides were separated on an Acclaim PepMap™ RSLC column (15 cm × 50 μm inner diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Neuroscience 2019Quote: ... 12mm circular glass coverslips containing cells were incubated at room temperature for 20 min with the Ca2+-sensitive dye Fura-2-AM (Invitrogen, 2 μM), dissolved in standard extracellular HEPES-buffered HBSS (known hereafter as extracellular imaging buffer ...
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bioRxiv - Microbiology 2021Quote: ... incubated in primary antibody (anti-SARS-CoV-2 Spike, which detects the S2 subunit of SARS-CoV-2 S (Invitrogen, PA1-41165), anti-GAPDH (proteintech ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cell Biology 2020Quote: ... 2% BSA and 2 mM EDTA at 1 x 106 cells per 100 μl: anti-ITGA7 APC 1:500 (Invitrogen MA5-23555) and anti-SCA-1 FITC 1:50 (BD Pharmingen 557405) ...
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bioRxiv - Immunology 2020Quote: ... for 2 days, then passaged 1:2 and cultured overnight in R10 medium (1X Roswell Park Memorial Institute (RPMI, (Life Technologies SAS), 10% fetal calf serum ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Cell Biology 2021Quote: Amnion and choriodecidua explants (2 cm2) were placed in six-well plates with 2 ml of DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: Amnion and choriodecidua explants (2 cm2) were placed in six-well plates with 2 ml of DMEM/F12 (Gibco, Thermo Fisher Scientific) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Genetics 2020Quote: ... A single gRNA and single strand HDR oligo were co-transfected along with the gRNA (1-2 μg of each plasmid) into 1-2 × 106 WT ESCs using Lipofectamine 2000 (Invitrogen #1168-019) in a single well of a 6 well plate ...
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bioRxiv - Immunology 2022Quote: ... and rested for 30 minutes at room temperature in a buffer consisting of 2% FBS in 1X PBS (2% FACS buffer) with 50nM dasatinib (Thermo Fisher Scientific). Cells were washed once with 2% FACS buffer with 50nM dasatinib and incubated with the Gag386-394GW9 and Nef103-111RM9 tetramers for 45 minutes at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Individual DGs were removed from the sting complex using clean forceps and pooled according to treatment group in sterilized 2 mL FastPrep tubes containing six 2 mm zirconia beads and 250 µL Trizol (Invitrogen, Carlsbad, CA). Individual DGs were pooled to obtain sufficient amounts of RNA for downstream analyses ...
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bioRxiv - Neuroscience 2022Quote: ... where (+T) is LNA] was diluted to a final concentration of 2 ng/µl in hybridization buffer [2× SSC (Thermo Fisher Scientific), 0.1 mg/ml yeast tRNA (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2022Quote: ... embryos at the desired developmental stage were fixed for one hour using MAD fixative (2 parts methanol [Thermo Fisher Scientific, Waltham, MA], 2 parts acetone [Thermo Fisher Scientific,
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Biophysics 2024Quote: ... Cell detachment for cryopreservation or experiments was achieved through 30 minutes of incubation with 5 mM EDTA in PBS without Ca+2 and Mg+2 (PBS-/-) followed by TrypLE Express dissociation agent (Gibco, 12605-010) for five minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...
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bioRxiv - Microbiology 2023Quote: The purified PCR products were cloned into pCR-XL-2-TOPO™ vectors using the TOPO™ XL-2 Complete PCR Cloning Kit (Invitrogen). After incubation ...