Labshake search
Citations for Thermo Fisher :
7601 - 7650 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: ... A square glass window (2 mm x 2 mm) of No 1 cover glass (0.13 to 0.17 mm thick, Thermo Fisher Scientific, USA) was placed over the craniotomy and the edges were sealed with cyanoacrylate glue (3M ...
-
bioRxiv - Molecular Biology 2020Quote: ... RD18 and JR1 cells were cultured for 2-5 days in DMEM supplemented with 2% horse serum (Gibco, Carlsbad, CA, USA) at 90-95% confluency ...
-
bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% fetal bovine serum (FBS)) and 30IU/mL recombinant human IL-2 (rhIL-2; Thermo Fisher Scientific, Waltham, MA).
-
bioRxiv - Neuroscience 2019Quote: ... Muscles were extensively washed with PBS for at least 2 hours and then incubated for additional 2 hours with appropriate secondary antibodies (Life Technologies) supplemented with fluorescently labelled α-bungarotoxin (Life Technologies ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were plated at a density of 58,000 cells/cm2 on PDL-coated 12 mm coverslips and maintained in neurobasal media supplemented with 2% B27 and 2 mM Glutamax (Life Technologies) at 37°C and 5% CO2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Japan), 50 μg/mL streptomycin (Meiji Seika Pharma, Japan) and 0.1 mM 2-mercaptoethanol (2-ME; 21985-023, Thermo Fisher Scientific). For the maintenance of PKA-ESCs ...
-
bioRxiv - Biochemistry 2020Quote: ... Primary antibodies were removed by 3 washes with 2% BSA 1x PBS and incubated with 2 μg/ml anti-rabbit IgG Alexa Fluor 594 (Thermo Fisher) for 2 h in the dark at 4°C ...
-
bioRxiv - Genetics 2021Quote: ... and 1.5 µg of each of the sgRNAs plasmids (PX330-Nanog-sgRNA1/2 and PX330-Esrrb-sgRNA1/2) using 16.5 µl of lipofectamine 3000 and 22 µl of P3000 (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2020Quote: ... cell pellet of 1 x106 – 2 x106 PBMCs was resuspended in 2 ml erythroblast cell expansion media containing basal blood media Stempo34 SFM (Thermo Fisher) supplemented with 1x Stempo34 nutrient supplement (Thermo Fisher) ...
-
bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
-
bioRxiv - Immunology 2020Quote: T or B cells (2-4 × 107) obtained from 2 spleens of actin-GFP mice by negative MACS kits (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2019Quote: ... were cultured to a density between 2*105 and 2*106 cells per ml in complete media [RPMI 1640 with HEPES and GlutaMAX (Gibco #72400120), supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS) ...
-
bioRxiv - Cell Biology 2021Quote: ... The agarase-treated samples were then carefully poured into combing reservoirs containing 1.2 ml of MES solution supplemented with 2 mM Zn(O2CCH3)2 and either S1 nuclease (40 U/ml) or S1 nuclease dilution buffer (Thermo Fisher) and were incubated for 30 min at RT ...
-
bioRxiv - Microbiology 2022Quote: ... Filters were first cut into 2 mm by 2 mm pieces using scissors sterilized and treated with RNase AWAY surface decontaminant (Thermo Scientific). Filter pieces were transferred into MN Bead Tubes Type A (Macherey-Nagel ...
-
bioRxiv - Biochemistry 2022Quote: ... were added to Au-flat 2/2 (2 µm hole, 2 µm space) cryo-electron microscopy grid (Protochips, Morrisville, NC, USA) using a Vitrobot Mk IV (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
-
bioRxiv - Microbiology 2022Quote: ... and the resulting peptides were collected on a trap column (2 mm × 2 cm) with POROS 20 R2 material (Thermo Scientific) at 0.5°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The following day cells were rinsed with PBS and incubated for 2 hours with the appropriate Alexa Fluor-conjugated secondary antibodies (2 μg/mL, Thermo Fisher) and DAPI (1 μg/mL ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and washed four times with PBS (2 x quick, 2 x 5 min) prior to incubating with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
-
bioRxiv - Biochemistry 2024Quote: ... were applied onto freshly glow discharged R 2/2 UltrAuFoil grids prior to plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) with a blot force of 0 and 5 sec blot time at 100% humidity and 22°C ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 µL of 1.1 mg/mL or 0.5 mg/mL of complex were loaded onto freshly glow discharged R 2/2 UltrAuFoil grids84 prior to plunge freezing using a Vitrobot Mark IV (ThermoFisher Scientific) with a blot force of 0 and 6 sec blot time at 100% humidity and 22°C ...
-
bioRxiv - Cancer Biology 2024Quote: ... the SunTag plasmids and HMGB1-BFP (2 dishes) or Smad3-BFP plasmid (2 dishes) were simultaneously transfected with Lipo3000 transfection reagent (ThermoFisher Scientific). One dish of cells transfected with HMGB1-BFP ...
-
bioRxiv - Cancer Biology 2024Quote: ... Organoid proliferation used the thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) for 2 hrs using Click-iT EdU Alexa Fluor-555 Imaging Kit (Invitrogen, C10338). H&E and IHC slides were viewed by brightfield-fluorescent microscopy (Olympus BX60) ...
-
bioRxiv - Neuroscience 2024Quote: ... NPCs were cultured in 2% B-27-supplemented Neurobasal CTS Media (plus 2 uM L-glutamine and 0.2% vol/vol penicillin–streptomycin; Thermo Fisher Scientific) plus 20 ng/ml BDNF ...
-
bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
-
bioRxiv - Immunology 2024Quote: ... Efferocytosis was quantified by incubating BMDMs with Jurkat T cells (MOI 2, 45 min) which had been labelled with calcein-acetoxymethyl (2 h, 37 °C, ThermoFisher Scientific) and exposed to UV light (305 nm ...
-
bioRxiv - Cell Biology 2024Quote: RNA was extracted from ∼2-5 mg of dechorionated 0–2 hr embryos per biological replicate using TRIzol Reagent (#15596026, ThermoFisher Scientific) and treated with1 μL TURBO Dnase (2 U/μL ...
-
bioRxiv - Immunology 2024Quote: ... Digestion was stopped by adding acetonitrile to 2% and trifluoroacetic acid to 0.3% and the beads were collected with DynaMag-2 Magnet (Thermo Fisher Scientific). Digested peptides were purified by loading the supernatant into C18 StageTips (58) ...
-
bioRxiv - Microbiology 2023Quote: ... One-tenth volume of cDNA (2 µl) was added to qPCR reactions containing target specific primers (Table 2) and SYBR green master mix (Applied Biosystems) as per manufacturer’s specification ...
-
bioRxiv - Microbiology 2022Quote: ... U-2 OS cells were transduced with lentiviral vector encoding shBmal1 and cells selected using 2 μg/ml of puromycin (Life Technologies).
-
bioRxiv - Microbiology 2023Quote: ... The resulting peptic peptides were collected on a trap column cartridge (2 mm x 2 cm) that was filled with POROS 20 R2 reversed-phase resin (Thermo Scientific) and kept at 0.5 °C for 3 min ...
-
bioRxiv - Genomics 2023Quote: ... were added into a fresh 2 mL microcentrifuge tube and washed with 1 mL of NPB using a DynaMag-2 magnet (#12321; ThermoFisher Scientific) for a total of three washes (1 min incubation/each) ...
-
bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 variants were identified by the targeted sequencing approach with the Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... sub-confluent HEK293T cells in 24-well plates were transfected with 500 ng of the psiCHECK-2 reporter plasmids mentioned above (G4Q27, G4M27, and native psiCHECK-2) using Lipofectamine 3000 (Invitrogen, ThermoFisher Scientific) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Genetics 2023Quote: ... RNA pellets were resuspended with 16 µL of nuclease-free H2O and treated by DNase (2 µL of DNase buffer and 2 µL of TURBO DNase; Thermo Fisher) at 37 °C for 1 h ...
-
bioRxiv - Immunology 2023Quote: ... The BALF cell pellet was treated with red cell lysis buffer and re-suspended in Hank’s Balanced Salt Solution (HBSS) supplemented with 2% fetal bovine serum (FBS) (2% FBS/HBSS; Gibco, CA, USA) for cell counting and flow cytometry analysis.
-
bioRxiv - Immunology 2023Quote: ... cells were stained at 37°C for 30 min with 2 µM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCF-DA; Thermo Fisher Scientific) or 1 µM MitoSOX (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2023Quote: ... First, cell detachment was attained using a cell dissociation solution (at 2:2:1 ratio, Cell Dissociation Buffer (Thermo Fisher Scientific): RPMI 1640 ...
-
bioRxiv - Genomics 2023Quote: ... total RNA from young livers were spiked with 2 µl of a 1:100 dilution of ERCC Ex-fold Mix 1 while that from old livers were spiked with 2 µl of 1:100 dilution of ERCC Ex-fold Mix 2 (Thermo Fisher). Exfold ERCC spike-ins are provided in two mixes ...
-
bioRxiv - Biochemistry 2023Quote: ... The peptic peptides thus generated were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies were incubated overnight at 4 °C (Table 2) or 2 hours at room temperature and then washed prior to Alexa fluorochrome-conjugated secondary antibodies (1:500, ThermoFisher Scientific) incubation ...
-
bioRxiv - Cell Biology 2023Quote: ... The culture dish was coated with 0.2% gelatin and the cells were cultured in endothelial growth medium (EGM)-2 complete media (#CC-3162, Lonza Clonetics, Fisher scientific) with EGM-2 SingleQuots supplement kit (CC-4176 ...
-
bioRxiv - Physiology 2023Quote: ... Sense and antisense sequences targeting daf-2 or aak-2 were amplified from N2 lysates and subcloned into donor vectors using Gateway Cloning Technology (Life Technologies). The final plasmids for the sense and antisense expression of daf-2 or aak-2 under the str-3 promoter were generated using Gateway Cloning Technology (Life Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... Digested ganglia were gently washed once in ‘basal medium’ and twice in ‘complete medium’ (Neurobasal A medium supplemented with 2% B27 [Invitrogen/Thermo Fisher], 2 ng/ml GDNF [Invitrogen/Thermo Fisher] and 10 ng/ml NT4 [PeproTech ...