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Citations for Thermo Fisher :
7751 - 7800 of 10000+ citations for 6 THIOPHEN 2 YL 1H INDAZOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Wild type OL61 glioma cells (5.0 x 105) were cultured in a 6-well plate in 1mL of media (DMEM, Thermofisher Cat# 12430054 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated in a 6 cm dish and 24 hours later plasmids were transfected with Lipofectamine 2000 (Invitrogen) based on manufacture’s protocol and then incubated for 24 hours before immunoprecipitation experiments.
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bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
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bioRxiv - Microbiology 2021Quote: Sub-confluent cells on coverslips in 6 well plates were transfected with 2μg of DNA using the Lipofectamine 3000 transfection system (Invitrogen). Eighteen to twenty-four hours post transfection cells were fixed with 4% PFA for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... MDCK cells were grown to 90-95% confluence in 6-well tissue culture plates in DMEM (ThermoFisher Scientific/Gibco) containing 10% FBS (ThermoFisher Scientific/Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... was dissolved under stirring in 15 mL of an alginate solution (10 mg/mL-1) formed in 0.1 M MES buffer at pH 6 (ThermoFisher). Sulfo-NHS (41 mg ...
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bioRxiv - Developmental Biology 2021Quote: ... A range of 1,000–11,000 cells per well was seeded into wells prefilled with 150 μL Essential 6™ (E6 medium) (Gibco, Carlsbad ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA/protein complexes were then electrophoreticed by 6% native polyacrylamide gel and transferred to nylon membrane (Thermo Fisher Scientific). RNA was cross-linked with a UV lamp at a distance of 0.5 cm from the membrane for 2 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5X105 cells were reverse transfected with 120 pmol of siRNAs in 6-well plates using Lipofectamine RNAiMAX (ThermoFisher Scientific). Cells were harvested for RNA extraction 72 hours after transfection ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were seeded into 6-well plates and transfection protocol was followed according to manufacturer’s instructions (Cat.#13778075, Invitrogen).
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Pathology 2022Quote: ... resulting from UA were determined via a membrane-permeant JC-1 dye (5, 50, 6, 60 -tetrachloro-1, 10,3,30 -tetraethylbenzimidazol-carbocyanine iodide, Molecular Probes). JC-1 dye accumulates in mitochondria in a potential-dependent manner ...
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bioRxiv - Molecular Biology 2022Quote: ... The size and integrity of the biotinylated RNA were checked on a 6% Urea gel (Thermo Scientific, Darmstadt, Germany). Immediately before use ...
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bioRxiv - Pathology 2022Quote: Pathogen-free MPT cells (BU-MPT immortalized cells) were plated in 6-well plates in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco) supplied with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and mrc2 was measured by qPCR (relative to 18s) on QuantStudio 6 Flex Real-time PCR system (Applied Biosystems).
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bioRxiv - Plant Biology 2019Quote: ... The RT-qPCR was conducted in triplicates on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with SYBR Green Master Mix (Vazyme Biotech) ...
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bioRxiv - Genetics 2019Quote: ... The chromatin with the antibodies was incubated with 6 μl of Dynabeads Protein G (Thermo Fisher Scientific, Cat# 10003D) for one hour ...
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bioRxiv - Systems Biology 2019Quote: ... The membrane inserts were placed into 6-well plates containing 1.5 ml maintenance medium consisted of F12/DMEM (Gibco) supplemented with N-2 Supplement (Gibco ...
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bioRxiv - Immunology 2019Quote: Serum IL-6 and IL-17A concentrations were assessed by ELISA according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2019Quote: ... HEK293T cells were seeded at a density of 0.5×106 per 6-well in low antibiotic growth media (DMEM with 10% FBS (Gibco), 0.1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: ... were prepared by growing for 6–12 h on CA then suspended in Dulbecco’s phosphate buffered saline (DPBS, Gibco) to an absorbance of 1.0 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Reactions were incubated for 60 min at room temperature and run on a 6% DNA Retardation gel (Invitrogen™) in TBE buffer ...
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... human embryonic kidney 293 (HEK 293)(ATCC) cells were seeded in a 6-well plate at 0.3×106 cells/well in DMEM (ThermoFisher) supplemented with 10% FBS and 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2020Quote: ... whereby the transfected population is selected for removal of HXGPRT and replacement with the complementation allele in 6-thioxanthine selection medium [177 µg/mL of 6-thioxanthine (TRC, cat# T385800) in 4.5 g/liter D-glucose in GlutaMAX DMEM (Gibco), with 1% dialyzed FBS (Omega Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfection of HCT116 cells (0.6×106 cells/well in 6-well plate) with siRNA oligonucleotides was performed using Lipofectamine RNAiMAX (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... 6-well plate format) mock- or virus-infected (MOI of 0.01) were extracted using TRIzol reagent (Thermo Fisher Scientific). Superscript® II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were plated at a density of 6 ×105 cells/cm2 and cultured in Neurobasal media (Invitrogen, Carlsbad, CA) supplemented with B-27 (Invitrogen) ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Zoology 2021Quote: ... and the cDNA was used for qPCR analysis using the QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems) and SYBR Green detection system (TOYOBO ...
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bioRxiv - Immunology 2020Quote: ... male C57BL/6 recipient mice were first primed intraperitoneally (i.p.) with 50 mg ovalbumin (OVA) in alum (Thermo Scientific) at 2:1 v:v ratio in 100 μL total volume ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were incubated with transfection complexes for 5-6 hours and then replated onto #1.5 coverslips (Warner Instruments) coated with Collagen IV (Gibco). Cells were imaged in DMEM/10% FBS ...
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bioRxiv - Genetics 2021Quote: ... Cells were plated at 0.3-0.4 × 106 in 1.5 ml media in 6-well plates-24 hours before transfection with either rCro or lipofectamine 3000 (ThermoFisher) or Lipofectin (99-102 ...
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bioRxiv - Immunology 2020Quote: ... animals previously injected with vaccine or PBS where injected 24 hrs before sacrifice in the same footpad with 30 μL of 0.5 mM 5-and 6-carboxyfluorescein diacetate succinimidyl ester (CFSE) (Invitrogen). For assessing migration after 24 hrs ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... Cells were plated at a density of 0.6×106 cells in a 6-well culture dish containing DMEM medium (Gibco), and transient transfection was achieved after 48h when reaching 80% confluency in a 1:3 ratio DNA (μg) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time PCR was performed in a 384-well format on a QuantStudio 6-flex Instrument (Applied Biosystems) using PowerUp™ SYBR™ Green Master Mix (#A25780 ...
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bioRxiv - Developmental Biology 2020Quote: ... qPCR reactions were performed in duplicate on an Applied Biosystems Quantstudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) using 4 ng cDNA ...
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bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
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bioRxiv - Bioengineering 2020Quote: ... 2.5 μM of CellTracker™ Orange 5-(and-6)-(((4-chloromethyl)benzoyl)amino)tetramethyl-rhodamine CMTMR (Molecular Probes, C2927) was used to label iNeuron cells ...
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bioRxiv - Physiology 2021Quote: ... Cells were treated with dexamethasone (1μM) or vehicle (0.01% ethanol) and lysed 6 or 24 hours later by adding 0.5ml TRIzol (Invitrogen, ThermoFisher, UK) following removal of medium ...
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bioRxiv - Physiology 2021Quote: ... Cells were treated with dexamethasone (1μM) or vehicle (0.01% ethanol) and lysed 6 or 24 hours later by adding 0.5ml TRIzol (Invitrogen, ThermoFisher, UK) following removal of medium ...
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bioRxiv - Cancer Biology 2020Quote: ... Heat-induced antigen retrieval was performed by incubating slides in a solution of 10 mM sodium citrate pH 6 (MilliporeSigma; SX0445-20) with 0.05% (v/v) Tween20 (ThermoFisher) at 98C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... For siRNA experimentsHEK293T cells (1.5×105 cells/ml) were seeded in 6-well plates and transfected with 150nM siRNA using 4μl Lipofectamine 2000 (Invitrogen) and 184μl OptiMEM ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids expressing TSC22D4 alleles and Akt1 were transiently co-transfected to Hepa1-6 cells using Lipofectamine 2000 (Invitrogen, #11668019) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... newly formed clusters were transferred into Low-attachment 6-well plates (Cat. No. 07-200-601, Thermo Fisher Scientific) and cultured with RPMI 1640 plus GlutaMAX + 1% (v/v ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Cell Biology 2021Quote: ... For transfection, 375 ng of synthetic sgRNA (TrueGuide™, CRISPR932782_SGM) along with 6 μL Cas9 mRNA (Invitrogen™, A29378), and 18 μL of the MessengerMAX™ reagent (Invitrogen™ ...
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bioRxiv - Cell Biology 2019Quote: ... at a ratio of 1 μg eYFP-PCNA to 0.1 μg hygromycin marker to 6 μl FuGENE6 according to the manufacturer’s instructions except that we washed cells with Opti-MEM (Invitrogen), transfected cells in Opti-MEM ...