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Citations for Thermo Fisher :
7151 - 7200 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by quantitative PCR on an ABI Prism 7500 real-time PCR system using Power SYBR Green PCR Master Mix (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and primers amplifying a fragment of DHX37 transcript (forward 5’-CCCGATATCGAGAAAGCCTGG-3’ ...
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bioRxiv - Microbiology 2019Quote: Quantitative reverse transcription-PCR (qRT-PCR) analysis of the metabolically active community was achieved with the StepOnePlus Real-Time PCR system (Applied Biosystems, Foster City, CA USA). Amplification reactions were in ...
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bioRxiv - Microbiology 2021Quote: Quantitative Real-Time PCR (qRT-PCR) reactions were run on ABI Step One Plus TM Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). The housekeeping gene 23S rRNA was used as an internal control to which the expression levels of the tested genes were normalized ...
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bioRxiv - Developmental Biology 2024Quote: ... and qRT-PCR was conducted on a Bio-Rad CFX96 real-time PCR machine using SYBR Green PCR Master Mix (Thermo Fisher Scientific, catalog no. 4309155), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The quality of the RNA was determined using a Nanodrop spectrophotometer with A260/230 ≥1.6 and A260/A280 set at ≥1.8 (Thermo Scientific, Wilmington, DE). The cDNA was generated from mRNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... parasitemia was determined by flow cytometry and drug susceptibility assays were set up in black 96-well microtiter plates (Thermo Scientific) with 0.1% starting parasitemia and 2% hematocrit in a final volume of 200 µl of medium ...
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bioRxiv - Biochemistry 2022Quote: ... 100ml of Sf9 cells were set at 1.5 × 106 cells/ml and transfected using DNA:Cellfectin II (Thermo Fisher Scientific, Waltham MA) lipid complex ...
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bioRxiv - Molecular Biology 2020Quote: The generated peptides were acidified with TFA to a final concentration of 0.8% and analyzed by capillary liquid chromatography tandem mass spectrometry with an EASY-nLC 1000 using the two-column set-up (Thermo Scientific). The peptides were loaded with 0.1% formic acid ...
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bioRxiv - Immunology 2022Quote: ... and then stained with anti–Foxp3 at 23°C for 30 min using a Foxp3/Transcription transcription factor buffer set (Invitrogen, ThermoFisher Scientific). Samples were acquired with BD FACSCelesta (BD Biosciences) ...
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bioRxiv - Genomics 2020Quote: ... cells were washed twice in ice cold staining Buffer and fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (ThermoFisher) per manufacturer’s recommendation ...
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bioRxiv - Immunology 2021Quote: ... 17-7311-81) and TNF-α (eBioscience, 12-7321-81)) were stained before fixation/permeabi-lization (Intracellular Fixation & Permeabilization Buffer Set, ThermoFisher).
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bioRxiv - Neuroscience 2019Quote: ... Human IgG and IgM was quantified in blood serum (1:1000 dilution) and culture supernatants (DILUTION) using the Human IgG/IgM total ELISA Ready-SET-Go! Kits (Invitrogen/eBioscience). All ELISAs were performed as per manufacturer’s protocols ...
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bioRxiv - Neuroscience 2019Quote: ... DNA fingerprinting profile was performed on both lines by qPCR detection of a reference set of SNP panel using the TaqMan assays from Life Technologies. The genome-edited iPS cell line shows identical SNP profile with the corresponding iPS cell line used for gene targeting ...
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bioRxiv - Microbiology 2019Quote: ... All NS3 fragments with FLAG-tag that included AA mutations and NS3 cDNA vector were digested with enzyme sets KpnI and XbaI (Thermo Scientific). Following the digestions ...
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bioRxiv - Immunology 2019Quote: ... Cells were fixed and permeabilised for intracellular staining using the eBioscience Foxp3/ Transcription Factor Fixation/Permeabilisation Staining buffer set (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... GMCSF cDNA was amplified using a Taqman probe/primer set and normalized to GAPDH (catalog numbers Hs00929873_m1 and Hs02758991_g1, respectively, and Taqman Fast Advanced master mix, Life Technologies, Carlsbad, CA).
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bioRxiv - Biochemistry 2021Quote: ... a parallel reaction monitoring regime (PRM) was used to select for a set of previously selected peptides on an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher Scientific) operating in positive mode and running Tune version 3.3 ...
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bioRxiv - Immunology 2021Quote: ... samples were centrifuged again for 10 min at 14,000 x g, resuspended in permeabilization puffer (FoxP3 Transcription Factor Staining Buffer Set, eBioscience™) reconstituted with 10 % Normal Goat Serum (NGS, ThermoFisher) and stained with primary antibodies against Gephyrin (#ab136343 ...
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bioRxiv - Biochemistry 2020Quote: The samples were analyzed using an HPLC-ESI-MS/MS system consisting of a high performance liquid chromatograph (nanoAcquity, Waters) set in line with an electrospray ionization (ESI) Orbitrap mass spectrometer (LTQ Velos, ThermoFisher Scientific). A 100 μm id X 365 μm od fused silica capillary micro-column packed with 20 cm of 1.7 μm-diameter ...
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bioRxiv - Cancer Biology 2021Quote: 3D breast acinar cultures were set up in an 8-well chamber cover glass plates (Nunc Lab-Tek, Thermo Fisher Scientific) or 12-well plates (Eppendorf ...
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bioRxiv - Cancer Biology 2021Quote: 3D breast acinar cultures were set up in an 8-well chamber cover glass plates (Nunc Lab-Tek, Thermo Fisher Scientific) or 12-well plates (Eppendorf ...
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bioRxiv - Plant Biology 2021Quote: ... Reactions were set in technical duplicates or triplicates in MicroAmp® Fast Optical 48-well 0.1 mL reaction plates (Applied Biosystems, Cat ...
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bioRxiv - Cancer Biology 2020Quote: Human cavin3 Stealth siRNAs (set of 3-HSS174185, 150811, 150809) and Human BRCA1 Stealth siRNAs (set of 3 - HSS101089, 186096, 186097) were purchased from Life Technologies Australia Pty Ltd ...
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bioRxiv - Cell Biology 2022Quote: The incubation reactions were set up in a microcentrifuge tube at room temperature before transferring to a Lab-Tek II chambered cover glass (Fisher Scientific) for imaging ...
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bioRxiv - Physiology 2022Quote: The branchiostegites were removed from the freshly killed crabs and the three posterior gill pairs were dissected and set aside in TRIzol reagent (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... were crossed with knockout strain in BY4741 background (MATa, his3Δ1; leu2Δ0; met15Δ0; ura3Δ0;gene::KANMX) (Yeast KO Library, Mat A complete set, Thermofisher Scientific cat no. 95401.H2) as described previously [50] ...
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bioRxiv - Developmental Biology 2021Quote: ... A target set of 20 short Alexa647 labelled oligo mouse Dux ViewRNA ISH probe (VB6-3223670-VC, type-6; ThermoFisher Scientific) was designed and used to hybridize to Dux RNA sequence ...
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bioRxiv - Neuroscience 2020Quote: ... phosphatase inhibitor cocktail sets II and III) (1-5 mg tissue per ml of lysis buffer) and homogenised using a Bead Mill (Fisher Scientific). Laemmli buffer (0.004% bromophenol blue ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Cancer Biology 2020Quote: ... Flow rate of nLC was set to 300 nL/min and coupled to Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher, USA) with 2.5 kV spray voltage and 275 °C of capillary temperature ...
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bioRxiv - Immunology 2022Quote: ... Intracellular staining was performed at room temperature using the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (ThermoFisher Scientific, Waltham, MA) as directed by the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... were detected after cell fixation and permeabilization with a Foxp3 Staining Buffer Set according to the manufacturer’s protocol (Thermo Fisher Scientific) using the following antibodies ...
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bioRxiv - Immunology 2020Quote: ... and then fixation and permeabilization using the eBioscience Foxp3/Transcription Factor Staining Buffer Set according to manufacturer’s instructions (Thermo Fisher Scientific). Antibodies used are listed in Table S3 ...
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bioRxiv - Cell Biology 2019Quote: ... followed by fixation in 2% PFA (panel 1 and 3) for 15 min or overnight fixation with eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (00-5523-00, Invitrogen) (panel 2) ...
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bioRxiv - Genomics 2020Quote: ... The instrument used was a Fourier transform near-infrared (FT-NIR) spectrometer set (Antaris™ II Model, Thermo Scientific Inc., USA), under the following operating conditions ...
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bioRxiv - Biochemistry 2021Quote: For the first set of experiments we used the Nunc™ MicroWell™ 96-Well Microplates (ThermoFisher Scientific, cat. n. 269620) with Nunc™ Microplate Lids (ThermoFisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Murine IFNγ and IL-2 cytokines were measured in supernatants according to the Murine IFNγ and IL-2 ELISA Ready-SET-GO!® ELISA kit (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Plasmids (Supplemental Data Set 1B) were constructed using classical cloning procedures and the GreenGate system (Lampropoulos et al., 2013) utilizing enzymes from Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... another aliquot for each TMT set was processed using the High-Select™ TiO2 phosphopeptide enrichment kit (both Thermo Fisher Scientific), according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... parasitemia was determined by flow cytometry and the drug susceptibility assays were set up in black 96-well microtiter plates (Thermo Scientific) with 0.1 % starting parasitemia and 2 % hematocrit in a final volume of 200 µl ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated overnight and IFNγ levels in the supernatant were detected using the human IFN-γ ELISA Ready-SET-Go! kit (Invitrogen) accorded to manufacturer’s instruction.
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bioRxiv - Developmental Biology 2021Quote: ... cells were then fixed and permeabilized using Invitrogen’s Transcription Factor Staining Buffer Set (Thermo Fisher Scientific; Cat. No.: 00-5523-00) according to manufacturer’s instructions and stained with Nur77 (12.14 ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed for 1 h at 4°C using a FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, for FOXP3 detection) or 15 min at room temperature using a 1.85% formaldehyde solution in PBS (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... and the sample incubated on ice while grid freezing commenced using a Vitrobot set at blot force −10 (Thermo Fisher Scientific). The samples were diluted 1:2 with cryo-EM buffer immediately before application of the protein sample to the grid ...
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bioRxiv - Immunology 2022Quote: BAL and NT samples were stained in 96-wells plates using the FoxP3 / Transcription factor staining buffer set (eBioscience, Thermo Fisher). Cells were stained with α-CD4-APC (02 ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were washed in FACS buffer and fixed and permeabilized with the Foxp3 / Transcription Factor Staining Buffer Set (00-5523-00, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed once with FACS buffer and subsequently fixed for 30 minutes with fixation/permeabilisation buffer of eBioscience Foxp3/transcription factor staining buffer set (ThermoFisher Scientific). They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4 ...
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bioRxiv - Physiology 2022Quote: ... The miRNA expression profiles were analyzed on paired samples using the TaqMan® Array rodent MicroRNA Card Set v3.0 (TLDA, Applied Biosystems) after pre-amplification steps ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time qPCR reactions were set up in triplicates with the Applied Biosystems Applied Biosystems SYBR green (Applied Biosystems; Cat#A25778) or TaqMan® Fast Advanced Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2024Quote: ... while the probe set for glula was designed using a custom script (Trivedi and Powell, unpublished) and ordered from Life Technologies, ThermoFisher ...