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Citations for Thermo Fisher :
7051 - 7100 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The RT-cDNA was generated using the Invitrogen™ SuperScript™ II Reverse Transcriptase (ThermoFisher), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and converted to cDNA using a using a SuperScript IV RT kit (Invitrogen, Cat. #18090050). The tRNA library was PCR amplified for 10 cycles using Illumina multiplex and barcode PCR primers and Platinum SuperFi II DNA Polymerase (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... Samples were shifted to RT and treated with 1 µl RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher) and 0.5 µl Thermo Scientific Maxima H Minus Reverse Transcriptase ...
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bioRxiv - Developmental Biology 2020Quote: ... Reverse transcription was performed with the High-Capacity cDNA RT Kit (Thermo Fisher Scientific #4368814). Quantitative PCR (qPCR ...
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bioRxiv - Immunology 2019Quote: ... First strand reverse transcription was performed using SuperScript III RT (200U/μl, Thermo Fisher Scientific). To the 10μl annealed sample ...
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bioRxiv - Immunology 2019Quote: ... mRNA was reverse transcribed (RT) using the TaqMan Reverse Transcription Reagents kit (Life Technologies; N8080234) with oligo (dT ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and synthesized cDNA using a RevertAid RT Reverse Transcription Kit (ThermoFisher Scientific, Waltham, MA, USA). We performed qRT-PCR using 20 ng cDNA per reaction with commercially available primer probe sets (Table 1) ...
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bioRxiv - Physiology 2019Quote: cDNA synthesis for mouse FST was performed by reverse transcriptase in a RT-qPCR (Invitrogen) mixed with mRNAs isolated from the ovary tissues of C57BL/6J mouse ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... RNA was reverse transcribed (RT) using Moloney murine leukemia virus (MMLV) reverse transcriptase (#28025013, Ambion) and a mixture of anchored oligo-dT and random decamers (IDT) ...
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bioRxiv - Microbiology 2020Quote: ... reverse transcription (RT) was performed using High Capacity RNA to cDNA kit (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... The reverse transcription (RT) Master Mix contained 2.2uL of 5X Maximus H-Reaction Buffer (ThermoFisher), 1.65uL of 50% PEG8000 (Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... RT mixes were prepared using the superscript IV reverse transcriptase kit (Life Technologies, CA, USA) and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... Nuclei were stained for 10 min at RT with Hoechst 3334 (H3570, Thermo Fisher Scientific) diluted 1:10,000 in PBS ...
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bioRxiv - Microbiology 2021Quote: ... and subjected to RT-qPCR reaction using the TaqMan Fast Advanced Master mix (Applied Biosystems) or SYBR Green/ROX qPCR Master Mix kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... RT was performed for 1 hour at 55°C with SuperScript III (Thermo Scientific, 18080051) using 5 μl of RNA-RT primers mix in 10 μl final volume (100 U Superscript III ...
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bioRxiv - Pathology 2020Quote: ... The RT-qPCR reaction used TaqPath 1-Step Multiplex Master Mix (Cat.# A28527, Thermo Fisher) along with 2019-nCoV RUO Kit (Cat.# 10006713 ...
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bioRxiv - Microbiology 2021Quote: ... The total RNA was then reverse transcribed (RT) using Maxima Reverse Transcriptase kit (Thermo Scientific) and ReadyMade Random Hexamers (Integrated DNA technologies [IDT]) ...
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miRNA activity contributes to accurate RNA splicing in C. elegans intestine and body muscle tissues.bioRxiv - Molecular Biology 2019Quote: ... the cDNA was synthesized from each sample using SuperScript III RT (Life Technologies, cat 18080093) according to the manufacturers protocol ...
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bioRxiv - Pathology 2019Quote: ... RT-qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, cat. # A25742) and the StepOnePlus using the relative standard curve method ...
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bioRxiv - Genetics 2020Quote: ... 500 ng of total RNA were used in a RT reaction with Superscript III (Invitrogen) using a mix of the following oligonucleotides ...
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bioRxiv - Genetics 2020Quote: ... during 10 min at RT and then mounted with anti-fading mounting medium (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using 1x Maxima SYBR Green/ROX qPCR Master Mix (Thermo Fisher) on the StepOnePlus with primers for Cyclophilin A ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR reactions were performed using TaqMan® Fast Virus 1-Step Master Mix (ThermoFisher) and 5 µl of isolated RNA as a template to detect a 132 bp sequence in the ORF1b/NSP14 ...
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bioRxiv - Physiology 2021Quote: ... and RT-qPCR was performed using the Power SYBR Green Master Mix Kit (Thermo Fisher). Expression of GAPDH was used to standardize the samples ...
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bioRxiv - Microbiology 2021Quote: ... and TaqPath™ 1-Step RT-qPCR Master Mix (Applied Biosystems, Foster City, CA, USA). PCR cycler conditions were 2 min at 95 °C ...
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bioRxiv - Immunology 2021Quote: ... Levels of cDNA were quantified by RT-qPCR using Taqman Gene Expression Assay (Applied Biosystems). Differences in cDNA inputs were corrected by normalization to HPRT1 cDNA levels ...
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bioRxiv - Pathology 2021Quote: ... and 1 µg was reverse transcribed with RT Maxima First Strand kit (K1642, Thermo Scientific). The q-PCR was performed on cDNA using Light Cycler (Roche) ...
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bioRxiv - Developmental Biology 2020Quote: ... After blocking for 2h at RT in CAS-Block (1:10 in PBST; ThermoFisher, #008120) blocking reagent was replaced by antibody solution (diluted in CAS-Block ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were counterstained for 10 min at RT with the intercalating dye Hoechst 33342 (Invitrogen) diluted 1:1,000 in PBS ...
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bioRxiv - Genetics 2021Quote: RNA was reverse transcribed to cDNA using a RevertAid RT Reverse Transcription Kit (ThermoFisher Scientific). Primers 5’-CAAGGTGATCTCCAGCTTCA-3’ and 5’-ACCGTTCGTGTATGCATGTT-3’ were used to amplify CPR ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-qPCR reactions (20 μl) contained 12.5 μl SYBR Select Master Mix (Thermo Fisher Scientific) and 0.25 μM (primer sequences shown in Supplementary Table S3 ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 1 hour incubation at RT with antibodies anti-rabbit Alexa-Fluor 488 (Invitrogen) and 5 minutes staining at RT with 2ng/μl of DAPI (Merck ...
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bioRxiv - Immunology 2020Quote: ... overnight at RT and lysed in B-PER Bacterial Protein Extraction Reagent (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... the reverse transcription (RT) reaction was performed using 200 U SuperScript III reverse transcriptase (Invitrogen). After RT ...
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bioRxiv - Developmental Biology 2021Quote: ... Reverse transcription was performed using the RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific – K1622). qRT-PCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438 ...
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bioRxiv - Cell Biology 2021Quote: ... RNA captured on bead was elucidated using 10 µl of RT reaction mixture (Thermo Fisher) (1× Maxima H Buffer ...
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bioRxiv - Cancer Biology 2020Quote: RT of nuclear RNA was performed using the High Capacity cDNA Reverse Transcription Kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Zebrafish roraa gene was cloned from zebrafish mRNA using SuperScript III RT-kit (Invitrogen #18080044) and amplified with the following primers ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR mix was prepared using the PowerUp SYBR Green Master Mix (Thermo Fisher Scientific). qPCR was performed using the QuantStudio 7 Flex System ...
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bioRxiv - Developmental Biology 2022Quote: ... The total RNA (2 μg) was reverse transcribed using SuperScript II ribonuclease H-RT (Invitrogen) in 20 μl reactions following instructions.
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at RT with fluorescently labelled goat anti-mouse (1:10000, Invitrogen) in 5% milk in PBS-T ...
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bioRxiv - Biophysics 2022Quote: ... blocked for 30 min at RT with CAS-BlockTM Histochemical Reagent (008120, Thermo Fisher Scientific), and incubated for an hour at RT with primary antibody against H3K27ac (1:2,000 ...
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bioRxiv - Genomics 2022Quote: ... spun down and washed with 5x Maxima RT buffer (Thermo Fisher Scientific, Waltham, MA, USA). The beads were resuspended in 200 μl reverse transcription (RT ...
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bioRxiv - Genetics 2022Quote: ... After the addition and mixing of 1 μl SuperScript™ II RT(18080-044, INVITROGEN), RT was performed at 42°C for 50 minutes before heat inactivation at 70°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Viral presence was assessed through RT-qPCR (StepOnePlus Real-Time, Applied Biosystems, Foster City, CA) by preparing 20μL reactions containing 5x HOT FIREpol EvaGreen qPCR Mix Plus (ROX ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR of mRNAs were performed using SuperScript III Reverse Transcriptase (Cat. #18080-044, Invitrogen) and PowerUp SYBR® Green master mix (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes from RT samples to be compared were then amplified using Platinum Taq (Invitrogen) with 26 PCR cycles ...
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bioRxiv - Physiology 2022Quote: ... Reverse transcription (RT) was performed on 3 µg total RNA using MMLV reverse transcriptase (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR experiments were performed with three biological replicates using a QuantStudio7 cycler (Applied Biosystems). Each biological replicate sample was run in two technical replicates ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... slides were stained with Phalloidin-AF647 for 1 h at RT (1:2000, Invitrogen, #A22283) and mounted with Vectashield Antifade mounting media with DAPI (Vector Laboratories ...