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Citations for Thermo Fisher :
7101 - 7150 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: RT-qPCR of isolated total RNA was performed using TaqMan miRNA assay reagent (Life Technologies). ΔΔCT value was calculated using Sn2841 and U18 snoRNA as endogenous controls.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... slides were stained with Phalloidin-AF647 for 1 h at RT (1:2000, Invitrogen, #A22283) and mounted with Vectashield Antifade mounting media with DAPI (Vector Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... The RT enzymes tested included Maxima H minus (TFS, EP0751) and SuperScript IV (Invitrogen, 18090050). In all cases ...
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bioRxiv - Microbiology 2023Quote: ... 4% PFA was used for 15 min at RT to fix the cells (Thermo Fisher). As a quenching solution we used 300 mM glycine (Serva ...
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bioRxiv - Immunology 2023Quote: ... Gene expression was quantified by RT-qPCR using available Taqman gene expression assays (Thermo Fisher). Expression of each gene was normalized to 18S and analyzed as fold change over mock samples.
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bioRxiv - Neuroscience 2022Quote: ... for 10min at RT and mounted in microscope slides with ProLong Gold antifade (P36930, Invitrogen) or Mowiol (sigma #81381 ...
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bioRxiv - Molecular Biology 2022Quote: RT-qPCR experiment on EBs was performed using TaqMan Gene Expression Master Mix (Life Technologies) in LightCycler 96 instrument (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were counterstained by Hoechst 33342 (Thermo Fisher Scientific, 2 μg/mL, 5 min, RT), and slides were mounted in ProLong Gold antifade mountant (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... and a RT Maxima H Minus cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific, Dardilly, France) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Expression of Cbln1 was quantified using RT-qPCR with Power SYBR Green Master Mix (ThermoFisher) and a Bio-Rad CFX 384 Real-Time System ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was conducted using 500 ng template on a StepOne Plus device (Applied Biosystems). For the analysis ...
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bioRxiv - Pathology 2023Quote: ... Gene expression was measured via RT-qPCR using PowerUp SYBR Green Master Mix (Applied Biosystems) on the QuantStudio3 Real-time PCR System (Applied Biosystems by Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... Reverse transcription was performed using the RevertAid RT Reverse Transcription Kit (Thermo Fisher Scientific – K1622). qRT-PCR was performed with the SYBR Green JumpStart Taq Ready Mix (Sigma – S4438 ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was produced via reverse transcription (RT) of the resulting preparations by SuperscriptIII™ (Invitrogen), primed by random hexamers ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA from the RT reaction was purified using DynaBeads MyOne Silane Beads (Thermo Fisher Scientific) and amplified for 12 cycles using Amplification mix and primers provided in the Single Cell 3’ reagents module 1 (10X Genomics).
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using Fast SYBR Green master mix (Thermo Fisher scientific, Waltham, MA) in a 7500 Fast Real-time PCR system (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... an aliquot of the RT reaction was analyzed with PowerUp SYBR Green (Applied Biosystems, #A25777) a QuantStudio™ 6 Flex real-time PCR system (Applied Biosystems) ...
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bioRxiv - Bioengineering 2023Quote: ... The extracted RNA was then reverse transcribed using RevertAid RT Kit (Thermo Fisher, Cat # K1691) with random hexamer primers at 25°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was done using the PowerUP SYBR Green mastermix (Applied Biosystems, catalogue no. A25742) as recommended by the provider for a 10 μL reaction volume and 2 μL diluted cDNA (1:10 dilution) ...
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bioRxiv - Genetics 2023Quote: ... Complementary DNA was generated from 1 μg of total RNA with Superscript II RT (Invitrogen) and random hexamer primers (Promega) ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription (RT) was performed with Verso cDNA Synthesis Kit (Thermo Scientific #AB-1453/A) as per the manufacturer’s instructions using 100ng of total cellular RNA ...
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bioRxiv - Neuroscience 2023Quote: ... they were probed for 1h at RT with the fluorescent secondary antibodies (1:1000: ThermoFisher). Cell nuclei were stained with Hoechst 33342 (10 μg/mL ...
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bioRxiv - Genomics 2023Quote: ... for 2 h at RT followed by nuclear counterstaining with Hoechst 33342 (Invitrogen, 1:8000) for 30 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and reverse transcribed using Maxima First Strand cDNA Synthesis Kit for RT-qPCR (Thermo Scientific). qPCR was performed using Power-up SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... reverse transcription was carried out using RevertAid RT Reverse Transcription Kit (Thermo Fisher CN: K1691) and run on SimpliAmp Thermal Cycler (Thermo Fisher CN ...
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bioRxiv - Immunology 2023Quote: ... The RT step was performed using the SuperScript® III First-Strand Synthesis System (Invitrogen). FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Genomics 2022Quote: ... on Day 12 of reprogramming and reverse transcribed using the Maxima RT kit (ThermoFisher K1672). 20ng of reverse transcribed RNA was mixed with TaqMan™ Gene Expression Master Mix (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by RT-qPCR using Power SYBR Green Master Mix (Thermo Fisher Scientific, Cat# 4368577) and primers in final concentration of 0.2μM on an ABI 7900HT fast real-time PCR system ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR reaction were performed using the TaqMan® Fast Advanced Master mix (Applied Biosystems). IL-10 mRNA levels were determinated using gene specific primers (Hs00961622_m1 ...
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bioRxiv - Cell Biology 2023Quote: ... incubated at RT for 45 min on a shaker and washed 3x with PBS (Gibco) 10 min each ...
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bioRxiv - Cell Biology 2024Quote: ... Transcript levels were analysed by RT-qPCR using TaqMan Gene Expression AssaysTM (Thermo Fisher Scientific). This was done by using cDNA from 10 ng total RNA with 2x FastStart Universal Probe Master ROX (Roche ...
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bioRxiv - Microbiology 2024Quote: ... RNA extracted from swabs underwent RT-qPCR analysis (TaqPath COVID-19 CE-IVD Kit, ThermoFisher) to confirm SARS-CoV-2 infection and assess the presence of S-gene target failure (SGTF) ...
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bioRxiv - Cell Biology 2024Quote: ... the complementary DNA (cDNA) was synthesized by Prime Script RT Master Mix (Thermo Fisher Scientific), and a Taqman Master mix kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... based on retention time (RT) acquired from Compound Discoverer 3.2 and Xcalibur (Thermo Fisher Scientific), and compared with RT of the standards ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed in technical quadruplicate with TaqMan Fast Advanced Master Mix (Applied Biosystems) and TaqMan probes on an Applied Biosystems StepOnePlus ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated for 1 hr at RT in the dark in secondary antibodies (Invitrogen). For EdU incorporation ...
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bioRxiv - Neuroscience 2024Quote: ... The RT-qPCR was then performed with the TaqMan fast advanced master mix (4444557, ThermoFisher) or the Sensitive RT HS-PCR Mix (2017-2000 ...
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bioRxiv - Developmental Biology 2024Quote: ... for 5 minutes at RT before mounting the stained tissue with mounting medium (Invitrogen, P36961). Table S3 provides detailed information on the primary Abs used ...
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bioRxiv - Molecular Biology 2024Quote: ... For RT-qPCR After removing genomic DNA contamination by treatment with amplification grade DnaseI (Invitrogen), 2 µg of RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-qPCR was performed using PowerUp SYBR Green Master Mix (Thermofisher Scientific, Switzerland, cat. A25742) and primers with specific sequences as mentioned in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... Indicated genes were analyzed by RT-qPCR using Fast SYBR Green Master Mix (ThermoFisher; 4385612). The sequences of the primers utilized for each gene are listed in the Supplementary Table 5.
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were further used to synthesize cDNA with MMLV-RT enzyme (Invitrogen) and oligo-dT as primer following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... reverse transcription (RT) was performed using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) with random primers according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using SYBR Select Master Mix for CFX (Applied Biosystems, Cat. 4472942) using the Bio-Rad CFX Connect Real-Time System ...
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bioRxiv - Immunology 2024Quote: ... and resuspended in 10 µL of RT mix [1× First Strand buffer (Thermo Fisher Scientific), 1 mM dNTP ...
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bioRxiv - Cell Biology 2020Quote: Quantitative PCR (qPCR) was performed on a QuantStudio 7 PCR system (Applied Biosystem) with Power SYBR® Green PCR Master Mix (Life Technologies, Carlsbad, CA, USA) using the following program ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting complementary (c)DNA was used for quantitative real-time PCR (qRT-PCR) using SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... Quantitative real-time PCR analysis was conducted on an ABI 7500 Real-Time PCR system using PowerSYBR® Green PCR Master Mix (Thermo Fisher Scientific, Waltham, USA). Primer-specific annealing temperatures were pre-evaluated prior to the study to optimize PCR conditions ...