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Citations for Thermo Fisher :
6951 - 7000 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were carried out in ProFlex PCR system (Applied biosystems, Thermo Fisher Scientific, USA). All the PCR amplifications were performed in a reaction volume of 20 μL PCR mix containing 2 μL DNA (50 ng/μL)/ 5 μL cDNA (20 ng/μL ...
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α-synuclein expression in response to bacterial ligands and metabolites in gut enteroendocrine cellsbioRxiv - Neuroscience 2023Quote: ... Quantitative real-time PCR was conducted with a StepOne Real-Time PCR system (Applied Biosystems) using PowerUp™ SYBR™ Green Master Mix and gene specific (BLASTN ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR analysis was carried out using the SYBR Green PCR Master Mix (Applied Biosystems), with 0.3 μM primers ...
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bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed on an ABI PRISM 7500 real-time PCR system (Applied Biosystems) to obtain melt curves ...
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bioRxiv - Developmental Biology 2023Quote: ... TaqMan-based quantitative PCR was performed using the StepOnePlus Real-Time PCR System (Applied Biosystems). All samples were normalized to the expression level of bActin or RN18s ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR was performed using 1X Power SYBR-Green PCR Master Mix (ThermoFisher), with validated gene-specific primers (Qiagen ...
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bioRxiv - Bioengineering 2023Quote: ... qRT-PCR plates were run using the QuantStudio 5 Real-Time PCR system (Applied Biosystems) with a total reaction volume of 20 µL ...
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bioRxiv - Cell Biology 2023Quote: ... Quantitative PCR was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems; #4367659) in a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Real-time PCR was performed by the StepOnePlus/QuantStudio3 Real-Time PCR System (Applied Biosystems) using the TaqMan Gene Expression Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... These PCR amplicons were cleaned with a ChargeSwitch-Pro PCR cleanup kit (Invitrogen, Cat #CS32050). Amplicons were sonicated to ∼200 bp and sequencing libraries were prepared by the UNC high-throughput sequencing facility (HTSF ...
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bioRxiv - Microbiology 2023Quote: ... The PCR reaction was performed using a 7500 Fast Real-Time PCR System (ThermoFisher Scientific) at 95°C for 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... PCR fragments were cloned into the pJET 1.2 vector (CloneJet PCR Cloning Kit, Thermo Scientific) following the manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... qRT-PCR was performed on QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) with 384-well platform ...
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bioRxiv - Systems Biology 2023Quote: ... The PCR product was first purified using the GeneJET PCR Purification Kit (Thermo Fisher Scientific) to concentrate the DNA ...
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed on the StepOnePlus Real-Time PCR System (Applied Biosystems/Thermo Fisher) using the Fast SYBR Green Master Mix (Applied Biosystems/Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: The resulting PCR product was purified using GeneJET PCR Purification Kit (Thermo Fisher Scientific, USA) according to the manufacturer’s manual and directly cloned in the pJET1.2/blunt cloning vector to generate GFP-pJET plasmid (Table1).
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bioRxiv - Cancer Biology 2023Quote: ... The regions of interest were amplified by PCR using DreamTaq Green PCR Master Mix (ThermoFisher) according to the manufacturer’s recommendations (see Table S1 for primer sequences) ...
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bioRxiv - Developmental Biology 2023Quote: ... qRT-PCR was performed on the StepOnePlus Real-Time PCR System (Applied Biosystems/Thermo Fisher) using the Fast SYBR Green Master Mix (Applied Biosystems/Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... Quantitative PCR was performed in a QuantStudio 7 Flex Real-Time PCR System (ThermoFisher, MA). The sequences of qPCR primers are listed in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR was performed using the QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific), and the Ct values of Zcchc3 were normalized to the mean values obtained using ACTB as a housekeeping gene (ΔΔCt method).
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... PCR runs were performed using a QuantStudioTM 3 Real-Time PCR System (Thermo Fisher Scientific). The following primers were used ...
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bioRxiv - Cancer Biology 2022Quote: ... The quantitative PCR was performed by the miRCURY LNA SYBR Green PCR KiT (Applied Biosystems) and analyzed by CFX ConnectTM Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... The PCR products were then purified with the GeneJET PCR Purification Kit (Thermo fisher scientific) and diluted to 30 ng/uL ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR were performed using Quant studio 6 flex real time PCR from Life Technologies.
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bioRxiv - Cancer Biology 2023Quote: ... PCR 2 was performed using Phusion High-Fidelity PCR Master Mix (Thermo Scientific, MA, USA). Samples were multiplexed by adding an additional sample barcode during the second round of PCR ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... PCR fragments were purified using the GeneJET PCR Purification Kit (Thermo Fisher Scientific; Vilnius, Lithuania) and concentrations were measured by NanoDrop 2000 spectrophotometer ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR was performed using SYBR Green PCR Master Mix (Thermo Scientific, Waltham, MA). Relative gene expression was calculated using a 2-ΔΔCt method by normalization with GAPDH ...
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bioRxiv - Cancer Biology 2023Quote: Samples for quantitative PCR were prepared with 1X SYBR Green PCR Master Mix (Applied Biosystems) and various primers (primer sequences are listed in Supplementary file 2) ...
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bioRxiv - Immunology 2023Quote: ... and qRT-PCR was performed using Taqman 2x universal PCR master mix (Thermofisher; Cat# 4318157) with appropriate primer sets ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR was performed using Power SYBR Green PCR Master Mix (ThermoFisher Scientific, Cat# 4367659) on an Applied Biosystems AB7500 real time PCR instrument ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT-PCR was performed using QuantStudio™ 3 real-time PCR system (Applied Biosystems, USA) and PowerUp SYBR Green Master Mix of Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time PCR was performed using Taqman Universal PCR Master Mix (Thermo Fisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: PCR products were purified using an ExoSAP-IT PCR product cleanup reagent (Thermo Fisher Scientific). DNA sequencing reactions were performed using a BigDye v3.1 cycle sequencing kit (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... qRT-PCR was performed on QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific) with 384-well platform ...
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bioRxiv - Biophysics 2024Quote: ... Real time PCR were carried out on the 7500 Real-Time PCR System (Thermo Fisher), using pre-PCR step of 20 s at 95 °C ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... Oligo pools were PCR-amplified using Phusion Flash High-Fidelity PCR Master Mix (ThermoFisher Scientific) according to the manufacturers protocol with 1 ng/μL sgRNA template DNA ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed using the Power SYBR Green PCR Master Mix (Applied Biosystems, #4367659) with 10 ng of cDNA and 250 nM final primer concentration on a QuantStudio3 qPCR machine (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... qRT-PCR reaction was measured and processed using StepOne PCR system and software (Applied Biosystems). Relative quantification of gene expression was calculated to housekeeping gene GAPDH ...
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bioRxiv - Neuroscience 2023Quote: ... and by Repeat-Primed PCR using 2X Phusion Flash High-Fidelity PCR Mastermix (Thermo Fisher). End-point PCR was achieved by amplifying across the repeat region where the presence of a single band at the expected size indicated a non-expanded Wild-Type locus ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR was performed on a QuantStudio 6 Real-Time PCR System (Thermo Fisher). Primers used in Real time-qPCR experiments are listed in Supplementary Table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... The real-time quantitative PCR was performed on 7500 real-time PCR system (Applied Biosystems). The primers used for qPCR ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR reaction was carried out on QuantStudio 3 Real-Time PCR System (Thermo Fisher, A28132) and thermal cycling conditions included 50°C for 2 min and an initial denaturation step of 95°C for 10 min and 40 cycles at 95°C for 15 s and 60°C for 60 s ...
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bioRxiv - Immunology 2023Quote: ... Quantitative PCR was performed in a QuantStudio 7 Flex Real-Time PCR System (ThermoFisher, MA). The sequences of qPCR primers are listed in Table S6 ...
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bioRxiv - Genetics 2024Quote: ... Quantitative real-time PCR was performed with the 7500 Real-Time PCR System (Applied Biosystems). The mRNA level was normalized to GAPDH and expressed as fold change ...
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bioRxiv - Plant Biology 2023Quote: ... PCR was performed using the Phire Plant Direct PCR Master Mix kit (Thermo Fisher Scientific). The rest of the seedling was preserved in 4% paraformaldehyde for microscopy ...
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bioRxiv - Physiology 2023Quote: ... Quantitative PCR reactions were performed using the StepOnePlus real-time PCR cycler (Thermo-Fisher Scientific) and relative gene expression was calculated by normalisation to ACTB gene expression (2-ΔCt) ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR was performed with Scientific QuantStudio 5 and 7 Real-Time PCR System (Thermo Fisher) using the DyNAmo Color Flash SYBR Green Mix (Thermo Fisher) ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed on the StepOne Plus real-time PCR system (Applied Biosystems, USA) using Fast SYBR Green Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Quantitative real-time PCR was performed on a Viia7 real-time PCR machine (Thermo Fisher). Gene expression was normalized to RPL13 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantitative PCR was carried out with the Sybr Green PCR Master Mix from Applied Biosystems on an ABI PRISM 7900HT instrument ...