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Citations for Thermo Fisher :
7001 - 7050 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR (qPCR) was performed on a StepOne Plus Real Time PCR machine (Applied Biosystems). cDNA samples were analysed using power SYBR green mastermix (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... it was cloned into the pCR Blunt vector with a ZeroBlunt PCR cloning kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative PCR (qPCR) was carried out on a StepOnePlusTM Real Time PCR System (Thermo Scientific) using the PowerUPTM SYBRTM Green Master Mix (Applied Biosystems #A25741) ...
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bioRxiv - Neuroscience 2024Quote: ... quantitative PCR was carried using the StepOne Real-Time PCR System (Applied Biosystems, Tokyo, Japan) with the Lenti-X qRT-PCR Titration Kit (Takara Bio Inc. ...
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bioRxiv - Microbiology 2024Quote: ... Real-time PCR was performed using the SYBR Green PCR Master Mix (ThermoFisher Scientific, 4364346) and the primers described in Table 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative reverse-transcription PCR was performed using QS6 PCR System (Applied Biosystems, Carlsbad, CA, USA) using SYBR Green Master Mix (Thermofisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Real-time PCR was performed in StepOnePlus™ Real-Time PCR Systems (Applied Biosystems, USA). The PCR conditions were 5 min at 95 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative real-time PCR (qPCR) was performed with SYBR Green PCR Master Mix (Applied Biosystems) using the BioRad CFX Opus 384 qPCR system utilizing primers targeting genes of interest (Supplementary Table 2) ...
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bioRxiv - Microbiology 2024Quote: ... The quantitative Real-Time PCR was performed on StepOne Real-Time PCR Systems (Thermo Scientific) using SsoFast EvaGreen Supermix (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time quantitative PCR was conducted with Power SYBR Green PCR Master Mix (Applied Biosystems) using the StepOne real-time PCR system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... qRT-PCR was performed on the StepOne Plus real-time PCR system (Applied Biosystems, USA) using Fast SYBR Green Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... PCR was performed with Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific). Primers used to genotype RanBP9-TurnX mice were ...
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bioRxiv - Cell Biology 2024Quote: Quantitative real-time PCR was performed on a Viia7 real-time PCR machine (Thermo Fisher). Gene expression was normalized to RPL13 ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was carried out with the Superscript III RT first strand synthesis kit (Invitrogen) following DNase elimination of genomic DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated for 2–3 min at RT in trypsin (0.05% v/v; Gibco 25300-054), and tapped to release ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibody incubations were for 1 hour at RT using fluorescently tagged antibodies from Invitrogen. All antibody dilutions were made in a solution of 1 mg/ml BSA in 0.25% Tween 20 in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was then reverse-transcribed from the total RNA using MultiScribe RT kit (Thermo Fisher). Real-time PCR was performed on Applied Biosystems StepOne Plus machine ...
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bioRxiv - Immunology 2021Quote: ... Sections were treated for 2 h at RT with secondary goat antibodies (1/400, Invitrogen): anti-rabbit-Al488 ...
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bioRxiv - Immunology 2020Quote: ... Sections were placed in RT stabilization buffer of Hank’s balanced salt solution (HBSS; Gibco 14175) containing 2 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcription buffer (see RTS method above) and Superscript III (1U/µl final) (Thermo Scientific) were performed at 75°C for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: Reverse transcription (RT) of RNA was done using SuperScript III reverse transcriptase (Thermo Fisher Scientific). The protocol recommended for the enzyme was used in either 10 or 20 μl reactions ...
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bioRxiv - Neuroscience 2021Quote: RT-QuIC reactions were performed in white 384-well plates with a clear bottom (Nunc). Recombinant α-synuclein (rPeptide ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated at RT with Alexa488-conjugated anti-mouse IgG antibody (Life Technologies, A28175) at 1:1,000 dilution for 30 minutes and counterstained with DAPI (2 μg/mL) ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by secondary staining for one hour at RT (1:250, Alexa-488, Invitrogen, A11008). Images were taken using a LSM 510 confocal microscope (Carl Zeiss ...
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bioRxiv - Molecular Biology 2021Quote: Reverse transcription (RT) was performed using the SuperScript IV reverse transcriptase Kit (Thermo Fisher Scientific) with 1µg of total RNA ...
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bioRxiv - Biophysics 2022Quote: The RT assay was performed with the EnzCheck Reverse Transcriptase Assay kit (Molecular Probes, Invitrogen), as described by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: The RT assay was performed with the EnzCheck Reverse Transcriptase Assay kit (Molecular Probes, Invitrogen), as described by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg total RNA was used for cDNA synthesis with Superscript III RT kit (Invitrogen). qPCR was performed on a Thermo QuantStudio 6 Flex Real-Time PCR machine ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated for 1 h at RT with Alexa-conjugated secondary antibodies (Molecular Probes). After two washes in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... After a 1 hour incubation at RT in PBS/10% Natural Goat Serum (NGS, Invitrogen)/1% Triton ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by RT-qPCR analysis with Power SYBR Green Master Mix (Thermo Fisher Scientific; 4368577) on an ABI 7900HT fast real-time PCR system ...
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bioRxiv - Plant Biology 2019Quote: ... Reverse transcription (RT) was performed using a RevertAid Reverse Transcription Kit (Thermo Scientific™, #K1691). For qRT-PCR on auxin induced genes ...
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bioRxiv - Microbiology 2019Quote: Reverse transcription (RT) reaction was performed using SuperScript IV Reverse Transcriptase kit (Invitrogen/ThermoFisher Scientific) with 11 µl of purified VLP nucleic acids sample and random hexamer oligonucleotides according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: Reverse transcription (RT) reaction was performed using SuperScript IV Reverse Transcriptase kit (Invitrogen/ThermoFisher Scientific) with 11 µl of purified VLP nucleic acids sample and random hexamer oligonucleotides according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... were dissected at room temperature (RT) in either Complete Schneider’s Media (Schneider’s Drosophila media [Thermofisher] with 15% Fetal Bovine Serum and 0.6X Pen-Strep solution ...
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bioRxiv - Genetics 2020Quote: ... and cDNA synthesis occurred on the bead using Maxima H-minus RT (Thermo Fisher Scientific) and the Template Switch Oligo ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by RT-qPCR analysis with Power SYBR Green Master Mix (Thermo Fisher Scientific; 4368577) on an ABI 7900HT fast real-time PCR system ...
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bioRxiv - Systems Biology 2020Quote: The template switch mix was prepared with 44 μL of 5x RT Buffer (Thermo Scientific), 44 μL of 20% Ficoll PM 400 (Sigma Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using the PowerUp™ SYBR™ Green Master Mix (Thermo Fisher) on a 7300 Real Time PCR System and 7300 System SDS RQ Study Software (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Thermo Scientific™ Verso™ 1-Step RT-qPCR Kits with low ROX (Thermo Scientific) was used for one step reverse transcription in an ABI 7500 qPCR thermocycler ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription (RT) was performed with the High-Capacity RNA to DNA kit (Applied Biosystems). Quantitative real-time PCR was performed on a Step-One Plus real-time system (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... DNaseI-digested RNA was reverse-transcribed to cDNA using the commercial RevertAid RT (ThermoFisher: K1621) according to the manufacturer’s specifications in a reaction volume of 20 µL ...
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bioRxiv - Physiology 2021Quote: ... cDNA was synthesized using 2 ug RNA with RevertAid RT Kit (Thermofisher, catalog number K1691). Maxima SYBR Green/ROX qPCR Master Mix (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... and reverse transcription was carried out using multiScribe High Capacity RT kits (Applied Biosystems, USA). All of these steps were carried out according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... centrifuged at 1000 rpm for 5 min at RT and resuspended in HBSS (Fisher Scientific) supplemented with 1% BSA (Carl Roth) ...
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bioRxiv - Neuroscience 2020Quote: ... RT was performed on 1 µg of RNA with the Superscript IV synthesis kit (Invitrogen). Quantitative PCR (qPCR ...
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bioRxiv - Developmental Biology 2021Quote: cDNA production was performed using the RevertAid H Minus RT cDNA Synthesis kit (ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... pellets were resuspended in 4 μL of Pre-RT buffer (RNase-free water (ThermoFisher 10977023), dNTPs (ThermoFisher R0194 ...
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bioRxiv - Molecular Biology 2020Quote: ... The ligated RNAs were subjected to RT by SuperScript III First-Strand Synthesis System (Invitrogen) with random hexamers fused with Illumina TruSeq adapters (5’-CAGACGTGTGCTCTTCCGATCTNNNNNN-3’) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were blocked for 1 hr at room temperature (RT) in BlockAid (Thermo Fisher Scientific) and incubated for 48 hr at 4°C with antibodies against phosphorylated S6 ribosomal protein (Ser240/244 ...