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Citations for Thermo Fisher :
6801 - 6850 of 10000+ citations for 5 Nitrofluorescein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 5 μL DNA and 6.5 μL nuclease-free water and 12.5 μL PCR MasterMix (Thermo Fisher Scientific). The conditions for each PCR reaction were ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg of total RNA was treated with DNase (DNA-free Kit for DNase Treatment & Removal; Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... followed by RT-PCR reaction using SYBR Green PCR Master Kit in QuantStudio 5 Systems (Applied Biosystems). Gene expression was normalized to GAPDH using the comparative CT method.
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bioRxiv - Biochemistry 2021Quote: ... The qPCR reaction was performed using QuantStudio 5 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) using the following program:
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bioRxiv - Biochemistry 2021Quote: ... The qPCR reaction was performed using QuantStudio 5 Real-Time PCR System (Applied Biosystems, Thermo Fisher Scientific) using the following program:
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bioRxiv - Cancer Biology 2019Quote: ... organoids were incubated for 20 minutes with 500 ml culture medium containing 5 µM calcein-green (Invitrogen). For the quantification of the organoid size and count ...
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bioRxiv - Cell Biology 2021Quote: ... and data were analyzed on a QuantStudio 5 Real Time PCR machine (Applied Biosystems, Foster City, CA). ΔCT values were normalized to housekeeping gene RPL13a.
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bioRxiv - Cell Biology 2021Quote: ... grown to 60% confluency at 37°C and 5%CO2 in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Plant Biology 2021Quote: ... followed by cell wall and DNA counterstaining using mPS-PI and Hoechst solution (5 μg/ml; Invitrogen) or SCRI Renaissance staining SR2200 ...
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bioRxiv - Biochemistry 2021Quote: ... PAs were loaded with X-Rhod-1 AM (5 μM, Thermo Fisher Scientific Inc., Waltham, MA, USA) for 30 minutes at 30°C ...
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bioRxiv - Biochemistry 2021Quote: ... staining overnight with isolectin GS-IB4 Alexa Fluor™ 488 conjugate (5 μg/mL; I21411, Life Technologies), anti-TAK1 (1:100 ...
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bioRxiv - Biochemistry 2020Quote: ... 5 U SUPERase•In™ RNase Inhibitor and 50 U SuperScript II RT (ThermoFisher Scientific, cat. 18064014). Reactions were incubated at 25°C for 10 min to allow partial primer extension ...
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bioRxiv - Biochemistry 2020Quote: ... After 5 washes with PBS cells were incubated with AlexaFluor555-conjugated anti-mouse secondary antibodies (Thermo Fisher) (1:500 dilution in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions mixes were first incubated on ice for 5 min before adding 1 µl BMOE (Thermo Fisher) to a final concentration of 0.64 mM ...
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bioRxiv - Cell Biology 2021Quote: Cells were spun down at 300g for 5 min and resuspended with Schneider’s Drosophila Medium (Gibco, #21720001) without serum supplementation at 60×104 cells/mL ...
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bioRxiv - Cell Biology 2021Quote: ... DAPI staining was done for 5 minutes followed by mounting with Prolong gold antifade mountant (Invitrogen, P36930).
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bioRxiv - Cell Biology 2021Quote: ... Sections were washed 3-5 times with TBSTX buffer and then mounted on Superfrost slides (Fisher Scientific) with Slow fade Diamond mounting solution (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Exon 4 and Exon 5 were amplified (Table M1) and cloned using pJET1.2 cloning kit (Thermo Fisher) before sequencing.
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bioRxiv - Cell Biology 2021Quote: ... Mouse ENKD1 shRNA (nucleotides targeting 403-423 bp, 5′-CGCTCACCCAAGTATGACAAT-3′) were cloned into pLKO.1 (Invitrogen).
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bioRxiv - Plant Biology 2020Quote: ... 1 mM EDTA and 5 mM DTT) with the addition of 1μl of Protease Inhibitor (Thermo Fisher) and 1 μl of RNAse inhibitor (RiboLock) ...
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bioRxiv - Plant Biology 2020Quote: ... nucleic acid staining was performed by vaccum-infiltrating a 5 μM of Sytox Orange (Thermo Fisher; S11368) solution ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg of purified RNA was treated by DNase from a DNA-free DNA removal kit (Ambion). 125 ng of treated RNA was used to synthesize cDNA by a qScript Felex cDNA synthesis kit (Quanta Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg of purified RNA was treated by DNase from a DNA-free DNA removal kit (Ambion). Reverse transcription and qRT-PCR were performed as described above ...
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bioRxiv - Microbiology 2020Quote: ... and BSR T7/5 cells (RRID:CVCL_RW96; (34)) were propagated in Dulbecco’s modified Eagle’s medium (ThermoFisher Scientific, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Microbiology 2020Quote: ... late schizonts of prMA-GFP were stained with DRAQ5 DNA stain (Thermo Fisher, 5 µM, 30 minutes). The culture was resuspended in PBS for imaging ...
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bioRxiv - Systems Biology 2021Quote: ... Migrated LCs were extracted from epidermal explant sheets cultured in media (RPMI, Gibco, UK, 5%FBS, Invitrogen, UK ...
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bioRxiv - Systems Biology 2020Quote: ... 5 mg/mL aprotinin) and were attached via centrifugation on coverslips (thickness #1.5, Thermo Fisher Scientific, CB00110RAC20MNT0) previously coated with poly-L-lysine (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in Express-Five media (Life Technologies) and infected with 0,5% vol/vol of generation 2 (V1 ...
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bioRxiv - Microbiology 2021Quote: ... They were maintained at 37°C and 5% CO2 in high-glucose DMEM (11-965-092, Gibco), supplemented with 10% fetal bovine serum (Genclone ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml of the prepared cultures were mixed 1:50 with ionic strength adjuster (ISA, Thermo Scientific) and measurements were started immediately monitoring the electric potential in mV every 5 s for a total of 60 min at room temperature under constant stirring ...
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bioRxiv - Microbiology 2021Quote: ... penicillin/streptomycin/L-glutamine and 5 mg/ml Geneticin™ (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and grown at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... Medium was replaced with phenol-red-free RPMI supplemented with 5 μg/mL propidium iodide (P3566, Invitrogen). The plate was sealed with a clear ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was synthesized with the 5' RACE System for Rapid Amplification of cDNA Ends kit (Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Neuroscience 2019Quote: ... Cell nuclei were counterstained with 0.5 μg/ml DAPI for 5 min (Thermo Fisher Scientific, Rockford, IL). The following primary antibodies were used ...
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bioRxiv - Pathology 2021Quote: ... These cells were grown in DMEM/F12 (D6421) supplemented with 5% FBS (GIBCO/ThermoFisher, Grand Island, NY) and maintained at 37°C in 5% CO2 ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... for an additional 5 min and the cells were washed with ice-cold TBS (Thermo Fisher Scientific) three times ...
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bioRxiv - Molecular Biology 2020Quote: ... Acclaim PepMap 100 nanoViper C18 column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 100 Å) was used as pre-column for sample loading ...
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bioRxiv - Molecular Biology 2020Quote: ... while the EASY-Spray C18 column (Thermo Scientific, 15 cm×75 μm ID, 3 μm, 5 μm) was used as the analytical column ...
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bioRxiv - Molecular Biology 2021Quote: 0.63 mmole (1 equivalent) of the nucleoside-5’-monophosphate free acid (Santa Cruz Biotechnology or ACROS Organics) and 5 equivalents of 2-aminoimidazole hydrochloride (Combi-Blocks ...
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bioRxiv - Molecular Biology 2021Quote: ... L-glutamine (5 mg/ml) in the presence 4 μg/ml Blasticidin S HCl (A11139-03, Invitrogen) and 35 μg/ml Zeozin (R250-01 ...
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bioRxiv - Molecular Biology 2021Quote: ... After blocking with assay buffer (5% bovine serum albumin; Sigma–Aldrich; 0.05% Tween ThermoFisher Scientific, Pittsburgh, PA), samples were initially screened at 1:100 dilution (then titrated to optimal dilutions ...
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bioRxiv - Microbiology 2019Quote: ... 5 to 6 seeds were pipetted into 120 x 120 mm square petri dishes (Thermo Fisher Scientific) containing half-strength Murashige and Skoog salts (MS) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5’-\56-FAM\ CCT ACC TTA ACC TCC C-3’ with Minor Groove Binder (MGB; ThermoFisher Scientific). PCR was performed using 10X Master Mix to yield a final volume of 25 µl and final working concentrations of 16.6mM (NH4)2SO4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Biological duplicates (5 x 106 cells per replicate) were homogenized in 1 ml of TRIzol Reagent (Invitrogen) and purified using the mirVANA miRNA isolation kit (Ambion ...
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bioRxiv - Biochemistry 2020Quote: ... The ThT fluorescence signal was measured every 5 min using the Varioskan plate reader (Thermo Scientific, Inc.) or FLUOstar Omega plate reader (BMG Labtech ...
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bioRxiv - Cancer Biology 2020Quote: ... ANA-1 cells were incubated in serum-free media with CellTracker Red CMTPX Dye (5 μM, ThermoFisher) for at 37 °C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were maintained at 37°C with 5% CO2 and culture in F-12K media (Gibco, #21127), with 10% horse serum and 5% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2021Quote: gDNA was harvested from 5 million cells for WT and Cdc42-KOclones (Invitrogen Purelink Genomic DNA Kit). The purified gDNA was then used as a PCR template for primers that flank the CRISPR cut site ...
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bioRxiv - Biophysics 2020Quote: ... 5% CO2 atmosphere in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% Penicillin-Streptomycin (Pen-Strep ...